MEF2C (NM_002397) Human Untagged Clone

CAT#: SC118667

MEF2C (untagged)-Human myocyte enhancer factor 2C (MEF2C), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEF2C
• Synonyms: C5DELq14.3; DEL5q14.3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002397, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGAGAAAAAAGATTCAGATTACGAGGATTATGGATGAACGTAACAGACAGGTGACATTTACAAAGA
  GGAAATTTGGGTTGATGAAGAAGGCTTATGAGCTGAGCGTGCTGTGTGACTGTGAGATTGCGCTGATCAT
  CTTCAACAGCACCAACAAGCTGTTCCAGTATGCCAGCACCGACATGGACAAAGTGCTTCTCAAGTACACG
  GAGTACAACGAGCCGCATGAGAGCCGGACAAACTCAGACATCGTGGAGACGTTGAGAAAGAAGGGCCTTA
  ATGGCTGTGACAGCCCAGACCCCGATGCGGACGATTCCGTAGGTCACAGCCCTGAGTCTGAGGACAAGTA
  CAGGAAAATTAACGAAGATATTGATCTAATGATCAGCAGGCAAAGATTGTGTGCTGTTCCACCTCCCAAC
  TTCGAGATGCCAGTCTCCATCCCAGTGTCCAGCCACAACAGTTTGGTGTACAGCAACCCTGTCAGCTCAC
  TGGGAAACCCCAACCTATTGCCACTGGCTCACCCTTCTCTGCAGAGGAATAGTATGTCTCCTGGTGTAAC
  ACATCGACCTCCAAGTGCAGGTAACACAGGTGGTCTGATGGGTGGAGACCTCACGTCTGGTGCAGGCACC
  AGTGCAGGGAACGGGTATGGCAATCCCCGAAACTCACCAGGTCTGCTGGTCTCACCTGGTAACTTGAACA
  AGAATATGCAAGCAAAATCTCCTCCCCCAATGAATTTAGGAATGAATAACCGTAAACCAGATCTCCGAGT
  TCTTATTCCACCAGGCAGCAAGAATACGATGCCATCAGTGTCTGAGGATGTCGACCTGCTTTTGAATCAA
  AGGATAAATAACTCCCAGTCGGCTCAGTCATTGGCTACCCCAGTGGTTTCCGTAGCAACTCCTACTTTAC
  CAGGACAAGGAATGGGAGGATATCCATCAGCCATTTCAACAACATATGGTACCGAGTACTCTCTGAGTAG
  TGCAGACCTGTCATCTCTGTCTGGGTTTAACACCGCCAGCGCTCTTCACCTTGGTTCAGTAACTGGCTGG
  CAACAGCAACACCTACATAACATGCCACCATCTGCCCTCAGTCAGTTGGGAGCTTGCACTAGCACTCATT
  TATCTCAGAGTTCAAATCTCTCCCTGCCTTCTACTCAAAGCCTCAACATCAAGTCAGAACCTGTTTCTCC
  TCCTAGAGACCGTACCACCACCCCTTCGAGATACCCACAACACACGCGCCACGAGGCGGGGAGATCTCCT
  GTTGACAGCTTGAGCAGCTGTAGCAGTTCGTACGACGGGAGCGACCGAGAGGATCACCGGAACGAATTCC
  ACTCCCCCATTGGACTCACCAGACCTTCGCCGGACGAAAGGGAAAGTCCCTCAGTCAAGCGCATGCGACT
  TTCTGAAGGATGGGCAACATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002397 unedited
  NGTCAGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGTTTGGAGG
  AACAGGAAAGAGAAAGAAAGGAAGGAAATAATACTAATTTCAGGGACGAGAGAGAGAAGA
  AAAACGGGGACTATGGGGAGAAAAAAGATTCAGATTACGAGGATTATGGATGAACGTAAC
  AAACAGGTGACATTTACAAAGAGGAAATTTGGGTTGATGAAGAAGGCTTATGAGCTGAGC
  GTGCTGTGTGACTGTGAGATTGCGCTGATCATCTTCAACAGCACCAACAAGCTGTTCCAG
  TATGCCAGCACCGACATGGACAAAGTGCTTCTCAAGTACACGGAGTACAACGAGCCGCAT
  GAGAGCCGGACAAACTCAGACATCGTGGAGACGTTGAGAAAGAAGGGCCTTAATGGCTGT
  GACAGCCCAGACCCCGATGCGGACGATTCCGTAGGTCACAGCCCTGAGTCTGAGGACAAG
  TACAGGAAAATTAACGAAGATATTGATCTAATGATCAGCAGGCAAAGATTGTGTGCTGTT
  CCACCTCCCAACTTCGAGATGCCAGTCTCCATCCCAGTGTCCAGCCACAACAGTTTGGTG
  TACAGCAACCCTGTCAGCTCACTGGGAAACCCCAACCTATTGCCACTGGCTCACCCTATT
  CTGCAGAGGAATAGTATGTCTCCTGGTGTAACACATCGACCTCCAAGTGCAGGTAACACA
  GGGTGGTCTGATGGGTGGAGACCTCACGTCTGGTGCAGGCACCAGTGCAGGGAACGGGTA
  TGGCAATTCCCGAATCTCACCAAGTCTGCTGGTCTCACCTGGTAACTGAACAAAATATGC
  AAGCACAATTTCTTCCCAATGATTTAGGAAGAATACCGTAACCAGTCTCCGAGTCTATAC
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002397 unedited
  TTTTAATATATAACACACATTNAGGNTCACNANAGGGNCCAAGGCTAGCATCCTTTATGG
  ATACAACCAAAGGTGCATTGTGGTCTAATTGTGGATTTCAGATCAAGTTAAGNAAAAATA
  TAGCCTAGTTAATAAGGTCTCTTGGCTTCTGCCACCCAGCGGCAGCCTTTTACCAAAAAT
  GGGNNNAGAAACACATAAGCAACTATGCAAGTAANANAAAGNNAAAAAAAAATGGATNNN
  NNNNNNNNNNAATGTNCTACNCNNNCCCNNNNTNNNNNNATNNNNNNANNNCACAGGTGG
  ATATTACTGGCTATATCTNCTTTGCTGGGTATGTCAATGCATATGATTGGAGATGAATCG
  ACAGATCTTCACATTCCAAGAGAAGGAAAATCTTTCCTAGACTGAAATATCTTTTAAACA
  ATATATATTGACTTTCTTATGGCACTCACTTATTAAAGGGTCTGAAAATAATCCCTTATA
  GACGAATATTANACAAGAGTCATTTTTTTTAATTAAAAAAAGGGACATAGAGAGGGTTCT
  TATGGGATTTCTTGAAATGGCTATTAAATCTCTTTATTTGAGAAAAAATAGATGACATAC
  ATGCTNTATGCANAAGTGACATTTGGGGGGTCCTGACACACCCGGGATTNGTCAACAGTC
  CTATATTGCTGACAAGATTGTCAAGGGTCANTTAAAAATATTTAAAACGGATGACAAAAC
  AACTATCCCTTTACCTGGCTTCTTTCTTGGCTAGCACCCAATCTTTTAAATTTTNTCTTC
  TGTGGGGAATTATAACCTTATTCACCCAAGTAAATTCCATATCACCTTGTTCCTAAGGCC
  ATTATTGCAGGAATTGGCCCCCAACTTAACTTTTTTTTTTGGGCATTTTTTAAAAACCTT
  TAAATTTTAGGTTGAAACACCCGATATTTTAAATTGGGCAGTTAGAATTGGCCTTCCGAA
  TCCAAAAGCTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002397
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002397.2, NP_002388.2
• RefSeq Size: 4077 bp
• RefSeq ORF: 1422 bp
• Locus ID: 4208
• Cytogenetics: 5q14.3
• Domains: MADS
• Protein Families: Transcription Factors
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe cognitive disability, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Variants 1, 6, and 9-11 all encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.