Manic Fringe (MFNG) (NM_002405) Human Untagged Clone

CAT#: SC118671

MFNG (untagged)-Human MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (MFNG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MFNG
• Synonyms: beta-1,3-N-acetylglucosaminyltransferase manic fringe; manic fringe homolog; MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; OTTHUMP00000043697; OTTHUMP00000043698; OTTHUMP00000043700
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002405 edited
  GAATTCGGCACGAGGGCCAACCAATGCAGTGCCGGCTCCCGCGGGGCCTGGCTGGAGCCC
  TCCTCACCCTCCTGTGCATGGGGCTCCTGTGTCTGCGGTACCACTTGAACCTGTCCCCGC
  AGCGGGTACAAGGGACCCCCGAGCTGAGCCAGCCGAACCCGGGGCCCCCTAAGCTACAGC
  TACACGATGTCTTCATTGCAGTGAAGACGACCCGGGCTTTCCACCGCTTGCGCCTGGAGC
  TGCTGCTTGACACGTGGGTTTCCAGGACCAGGGAACAGACATTTGTCTTCACCGACAGCC
  CAGACAAAGGCCTCCAGGAGAGACTGGGGTCCCACCTTGTGGTCACCAACTGCTCCGCGG
  AACACAGCCACCCAGCTCTGTCCTGCAAGATGGCTGCTGAGTTCGACACCTTCTTGGCCA
  GTGGGCTTAGGTGGTTCTGCCATGTGGACGATGACAACTATGTGAACCCAAGGGCGCTGC
  TGCAGCTTCTGAGAGCCTTCCCGCTGGCCCGCGACGTCTATGTGGGAAGGCCCAGCCTGA
  ACCGGCCCATCCATGCCTCAGAGCCACAGCCCCACAACCGCACGAGGCTGGTACAGTTCT
  GGTTTGCCACTGGGGGTGCTGGCTTCTGCATCAATCGCAAACTGGCTTTGAAGATGGCTC
  CGTGGGCCAGTGGCTCCCGTTTCATGGACACATCTGCTCTCATCCGGCTGCCTGATGACT
  GCACCATGGGCTATATCATTGAGTGCAAGCTGGGCGGCCGCCTGCAGCCCAGCCCCCTCT
  TTCACTCCCACCTGGAGACCCTGCAGCTGCTGAGGACTGCACAGCTCCCAGAACAGGTCA
  CCCTCAGCTACGGTGTCTTTGAGGGGAAACTCAACGTCATTAAGCTACAGGGCCCCTTCT
  CCCCGGAGGAGGACCCCTCCAGATTTCGCTCCCTCCATTGTCTGCTCTATCCAGATACAC
  CCTGGTGTCCCCAGCTGGGTGCCCGATGAATCCTGAACTGCTGGGCAAAGGTTGGGCAGA
  GACTTCTGGGTGTGCCTTGGCTCCCAAGGTGGCACTGTGGGTCCCTGGCAAGTGTCTTGT
  GATAGGCAGTCCCTGGCAGGGCCTTCGGGTGGTTGGCAAGCCCAGGATCTGAGTGGCAAT
  TGGCACTGAAGGCACCCCAGGCCCCTGGGAGGTGAGTTAGACAGCCCAGGGGACCAGGTG
  GACCAGGTGGTGGCCAGAGAGGCTCCAGGGGCTAGACTCCCTCAGGAGGCTGAATTGAAA
  AAGGGCAGGGGGCACTTGAGCTGGGCTGGGGCTCAGGGGTCCTAACCCTTTAGGCAGTGA
  CATGGCCTCTGGGTGGGGTCTGGCCGTTGGCCCTGGCTAATGTCTCTCAGTCATTCCCCT
  GGGGCTCAAGCGCTGGGCCGCCCACTCCTGCCTCCCTCATCTGTGTCCCGAGTTCCTGAA
  GGGACATGGGTGGAATGATGGCAGAATCCAGGGTCTGCAGCACCTGCTGTTGTTGCCAAC
  CAGTCTCCCAAAGCTCCTTGCTCCCCACCCCTTGCGAACAGGACCAGATTTTGTTTGGAG
  CCTCAGCATGCCGGGGCCCAGATGATGGAGCATAACGGGTCCCAGCCAATTGTGATGATC
  CTTTTTGCTCATTTCCCAGCCTTTCTTGCTGTTAGGGGCTACCATGGGACCAGCTCTGGC
  CAGAGGGAACTAAGCAAATCCAATAGAGATGTTTCTGGGGAAGGTTTTGCAGCCCACTCC
  CCATCTTCCTGCTATAAATGTGGGTGTGATGGCTGGATCTGGGGCAGCCACCTTGCTACC
  ATGAAGGAAAGGCCAAGACAATCATCCACAGCTATTCCCTCCAGCATCTGGTTCTGTACA
  AAAATTAAATGCTTATTTGTTTAAGTCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002405 unedited
  TGTGCAAATTTTGTNATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCCAA
  CCAATGCAGTGCCGGCTCCCGCGGGGCCTGGCTGGAGCCCTCCTCACCCTCCTGTGCATG
  GGGCTCCTGTGTCTGCGGTACCACTTGAACCTGTCCCCGCAGCGGGTACAAGGGACCCCC
  GAGCTGAGCCAGCCGAACCCGGGGCCCCCTAAGCTACAGCTACACGATGTCTTCATTGCA
  GTGAAGACGACCCGGGCTTTCCACCGCTTGCGCCTGGAGCTGCTGCTTGACACGTGGGTT
  TCCAGGACCAGGGAACAGACATTTGTCTTCACCGACAGCCCAGACAAAGGCCTCCAGGAG
  AGACTGGGGTCCCACCTTGTGGTCACCAACTGCTCCGCGGAACACAGCCACCCAGCTCTG
  TCCTGCAAGATGGCTGCTGAGTTCGACACCTTCTTGGCCAGTGGGCTTAGGTGGTTCTGC
  CATGTGGACGATGACAACTATGTGAACCCAAGGGCGCTGCTGCAGCTTCTGAGAGCCTTC
  CCGCTGGCCCGCGACGTCTATGTGGGAAGGCCCAGCCTGAACCGGCCCATCCATGCCTCA
  GAGCCACAGCCCCACAACCGCACGAGGCTGGTACAGTTCTGGTTTGCCACTGGGGGTGCT
  GGCTTCTGCATCAATCGCAAACTGGCTTTGAAGATGGCTCCGTGGGCCAGTGGCTCCCGT
  TTCATGGACACATCTGCTCTCATCCGGCTGCCTGATGACTGCACCATGGGCTATATCATT
  GAGTGCAAGCTGGGCGGCCGCCTGCAGCCCAGNCCCCTCTTTCACTCCCACCCTGGAGAC
  CCTGCAGCTGCTGAAGACTGCACAGCTCCCAGAACAGGTCACCCTCAGCTACGGTGTCCT
  TTGAGGGGAACCTCCACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002405 unedited
  TGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGACTTAAACAAAT
  AAGCATTTAATTTTTGTACAGAACCAGATGCTGGAGGGAATAGCTGTGGATGATTGTCTT
  GGCCTTTCCTTCATGGTAGCAAGGTGGCTGCCCCAGATCCAGCCATCACACCCACATTTA
  TAGCAGGAAGATGGGGAGTGGGCTGCAAAACCTTCCCCAGAAACATCTCTATTGGATTTG
  CTTAGTTCCCTCTGGCCAGAGCTGGTCCCATGGTAGCCCCTAACAGCAAGAAAGGCTGGG
  AAATGAGCAAAAAGGATCATCACAATTGGCTGGGACCCGTTATGCTCCATCATCTGGGCC
  CCGGCATGCTGAGGCTCCAAACAAAATCTGGTCCTGTTCGCAAGGGGTGGGGAGCAAGGA
  GCTTTGGGAGACTGGTTGGCAACAACAGCAGGTGCTGCAGACCCTGGATTCTGCCATCAT
  TCCACCCATGTCCCTTCAGGAACTNCGGACACANATGANGGAGGCAGGAGTGGGCGGCCC
  ANCGCTTGAGCCCCCAGGGAATGACTGAGAGACATTAGCCAGGGCCAACGGCCAGACCCC
  ACCCCAGAGCCATGTCACTGCCTAAAGGGTAGGACCCCTGAGCCCCAGCCCAGCTCAAGT
  GCCCCTGCCCTTTTTCAATCAGCCTCCCTGAGGAGTCTAGCCCCTGGAGCTCTCTGGCCA
  CCACCTGGTCCACCTGTCCCCTGGGCTGNCTACTTACCTCCCAGGGGCCTGGGTTGCCTC
  AGTGCCATTGCCACTCAAACCTGGGCTTGCCACCACCCAAAGCCCTGGCAGNACTGNCTA
  TACAGNAACCTGNCAGGGACCCAAAGGCACCTGGGNAGCCAGGCCACCCAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002405
• Insert Size: 1820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002405.2, NP_002396.2
• RefSeq Size: 2040 bp
• RefSeq ORF: 966 bp
• Locus ID: 4242
• Cytogenetics: 22q13.1
• Domains: Fringe
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• Gene Summary: 'This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the LFNG (GeneID: 3955) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene may control Notch signaling in claudin-low breast cancer. [provided by RefSeq, May 2018]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).