LOX (NM_002317) Human Untagged Clone

CAT#: SC118674

LOX (untagged)-Human lysyl oxidase (LOX), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LOX
• Synonyms: AAT10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118674 sequence for NM_002317 edited (data generated by NextGen Sequencing)
  ATGCGCTTCGCCTGGACCGTGCTCCTGCTCGGGCCTTTGCAGCTCTGCGCGCTAGTGCAC
  TGCGCCCCTCCCGCCGCCGGCCAACAGCAGCCCCCGCGCGAGCCGCCGGCGGCTCCGGGC
  GCCTGGCGCCAGCAGATCCAATGGGAGAACAACGGGCAGGTGTTCAGCTTGCTGAGCCTG
  GGCTCACAGTACCAGCCTCAGCGCCGCCGGGACCCGGGCGCCGCCGTCCCTGGTGCAGCC
  AACGCCTCCGCCCAGCAGCCCCGCACTCCGATCCTGCTGATCCGCGACAACCGCACCGCC
  GCGGCGCGAACGCGGACGGCCGGCTCATCTGGAGTCACCGCTGGCCGCCCCAGGCCCACC
  GCCCGTCACTGGTTCCAAGCTGGCTACTCGACATCTAGAGCCCGCGAAGCTGGCGCCTCG
  CGCGCGGAGAACCAGACAGCGCCGGGAGAAGTTCCTGCGCTCAGTAACCTGCGGCCGCCC
  AGCCGCGTGGACGGCATGGTGGGCGACGACCCTTACAACCCCTACAAGTACTCTGACGAC
  AACCCTTATTACAACTACTACGATACTTATGAAAGGCCCAGACCTGGGGGCAGGTACCGG
  CCCGGATACGGCACTGGCTACTTCCAGTACGGTCTCCCAGACCTGGTGGCCGACCCCTAC
  TACATCCAGGCGTCCACGTACGTGCAGAAGATGTCCATGTACAACCTGAGATGCGCGGCG
  GAGGAAAACTGTCTGGCCAGTACAGCATACAGGGCAGATGTCAGAGATTATGATCACAGG
  GTGCTGCTCAGATTTCCCCAAAGAGTGAAAAACCAAGGGACATCAGATTTCTTACCCAGC
  CGACCAAGATATTCCTGGGAATGGCACAGTTGTCATCAACATTACCACAGTATGGATGAG
  TTTAGCCACTATGACCTGCTTGATGCCAACACCCAGAGGAGAGTGGCTGAAGGCCACAAA
  GCAAGTTTCTGTCTTGAAGACACATCCTGTGACTATGGCTACCACAGGCGATTTGCATGT
  ACTGCACACACACAGGGATTGAGTCCTGGCTGTTATGATACCTATGGTGCAGACATAGAC
  TGCCAGTGGATTGATATTACAGATGTAAAACCTGGAAACTATATCCTAAAGGTCAGTGTA
  AACCCCAGCTACCTGGTTCCTGAATCTGACTATACCAACAATGTTGTGCGCTGTGACATT
  CGCTACACAGGACATCATGCGTATGCCTCAGGCTGCACAATTTCACCGTATTAG
  
  Clone variation with respect to NM_002317.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002317 unedited
  GTCAGATTTGTAAACGACTTCACTATAGGCGGCCCGCGAATTCGCACGAGGCAGACACTG
  CCCGCTCTCCGGGACTGCCGCGCCGCTCCCCGTTGCCTTCCAGGACTGAGAAAGGGGAAA
  GGGAAGGGTGCCACGTCCGAGCAGCCGCCTTGACTGGGGAAGGGTCTGAATCCCACCCTT
  GGCATTGCTTGGTGGAGACTGAGATACCCGTGCTCCGCTCGCCTCCTTGGTTGAAGATTT
  CTCCTTCCCTCACGTGATTTGAGCCCCGTTTTTATTTTCTGTGAGCCACGTCCTCCTCGA
  GCGGGGTCAATCTGGCAAAAGGAGTGATGCGCTTCGCCTGGACCGTGCTCCTGCTCGGGC
  CTTTGCAGCTCTGCGCGCTAGTGCACTGCGCCCCTCCCGCCGCCGGCCAACAGCAGCCCC
  CGCGCGAGCCGCCGGCGGCTCCGGGCGCCTGGCGCCAGCAGATCCAATGGGAGAACAACG
  GGCAGGTGTTCAGCTTGCTGAGCCTGNGCTCACAGTACCAGCCTCAGCGCCGCCGGGACC
  CGGGCGCCGCCGTCCCTGGTGCAGCCAACGCCTCCGCCCAGCAGCCCCGCACTCCGATCC
  TGCTGATCCGCGACAACCGCACCGCCGCGGCGCGAACGCGGACGGCCGGCTCATCTGGAG
  TCACCGCTGGCCGCCCCAGGCCCACCGCCCGTCACTGGTTCCAAGCTGCCTACTCGACAT
  CTAGAGCCCCGCGAAGCTGGCGCCTCGCGCGCCGGAGACCAGACAGCGCCCGGGAGAAGT
  TCCTGCGCTCAGTAACCCGCGGCCGCCCAACCGCGTGGACGGCATGTTGGGCCGACGACC
  CTTACAACCCCTACAAGTACTCTTGACGACAACCCTTTTATACCACTTACTACGATACTC
  ATTGAAAGGCCCCAACCTGGGGGCCAGTCCCCGGCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002317 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAAGATCAAATATGTAATTAC
  AGAATTGAAACACTGTGTTAATTCACAAAGTGATGTAAATAATAAACATTAAAAATTTCC
  CAAGTAATGATGACTTAAGCGTTCAAAATCCAGTTATGTGCTTTGTTATTGAAAACAGTC
  CAAACAAAAATTCTTTTGTTGTTTTCTGTTCTCTTTTTCAAAATACATAAATCCTACTGA
  AGTTAGTCTATTTTTTCCCACTTCAGAACACCAGGCACTGATTTATCCATTGGGAGTTTT
  GCTTTGCCTTCTAATACGGTGAAATTGTGCAGCCTGAGGCATACGCATGATGTCCTGTGT
  AGCGAATGTCACAGCGCACAACATTGTTGGTATAGTCAGATTCAGGAACCAGGTAGCTGG
  GGTTTACACTGACCTTTAGGATATAGTTTCCAGGTTTTACATCTGTAATATCAATCCACT
  GGCAGTCTATGTCTGCACCATAGGTATCATAACAGCCAGGACTCAATCCCTGTGTGTGTG
  CAGTACATGCAAATCGCCTGTGGTAGCCATAGTCACAGGATGTGTCTTCAAGACAGAAAC
  TTGCTCTGTGGCCTTCAGCCACTCTCCTCTGGGTGTTGGCATCAAGCAGGTCATAGTGGC
  TAAACTCATCCATACTGTGGTAATGCTGATGACAACTGTGCCATTCCCAGGAATATCTTG
  GTCGGCTGGGTAAGACATCTGATGTCCCTTGGTTTTTCACTCTTTGGGGAAATCTGAGCA
  GCACCCTGTGATCATAATCTCTGACATCTGGCCTGTATGCTGTACTGGCCAGACAAGTTT
  TCTCCGCCGCGCATCTCAAGTTGACATGGACATCTTCTGACGTACGTGAACGCCTGGTGT
  ATTAGGGGTCGCCCCCAGGCTGGGAAACCGCACTGGAAGTACCAGGCCGTATTCGGGCCG
  CACCTGCCCCCAGCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002317
• Insert Size: 1870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002317.3, NP_002308.2
• RefSeq Size: 1946 bp
• RefSeq ORF: 1254 bp
• Locus ID: 4015
• Cytogenetics: 5q23.1
• Domains: Lysyl_oxidase
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. In addition, defects in this gene have been linked with predisposition to thoracic aortic aneurysms and dissections. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.