LOXL2 (NM_002318) Human Untagged Clone

CAT#: SC118675

LOXL2 (untagged)-Human lysyl oxidase-like 2 (LOXL2)


  "NM_002318" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol LOXL2
Synonyms LOR; LOR2; WS9-14
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118675 sequence for NM_002318 edited (data generated by NextGen Sequencing)
ATGGAGAGGCCTCTGTGCTCCCACCTCTGCAGCTGCCTGGCTATGCTGGCCCTCCTGTCC
CCCCTGAGCCTGGCACAGTATGACAGCTGGCCCCATTACCCCGAGTACTTCCAGCAACCG
GCTCCTGAGTATCACCAGCCCCAGGCCCCCGCCAACGTGGCCAAGATTCAGCTGCGCCTG
GCTGGGCAGAAGAGGAAGCACAGCGAGGGCCGGGTGGAGGTGTACTATGATGGCCAGTGG
GGCACCGTGTGCGATGACGACTTCTCCATCCACGCTGCCCACGTCGTCTGCCGGGAGCTG
GGCTATGTGGAGGCCAAGTCCTGGACTGCCAGCTCCTCCTACGGCAAGGGAGAAGGGCCC
ATCTGGTTAGACAATCTCCACTGTACTGGCAACGAGGCGACCCTTGCAGCATGCACCTCC
AATGGCTGGGGCGTCACTGACTGCAAGCACACGGAGGATGTCGGTGTGGTGTGCAGCGAC
AAAAGGATTCCTGGGTTCAAATTTGACAATTCGTTGATCAACCAGATAGAGAACCTGAAT
ATCCAGGTGGAGGACATTCGGATTCGAGCCATCCTCTCAACCTACCGCAAGCGCACCCCA
GTGATGGAGGGCTACGTGGAGGTGAAGGAGGGCAAGACCTGGAAGCAGATCTGTGACAAG
CACTGGACGGCCAAGAATTCCCGCGTGGTCTGCGGCATGTTTGGCTTCCCTGGGGAGAGG
ACATACAATACCAAAGTGTACAAAATGTTTGCCTCACGGAGGAAGCAGCGCTACTGGCCA
TTCTCCATGGACTGCACCGGCACAGAGGCCCACATCTCCAGCTGCAAGCTGGGCCCCCAG
GTGTCACTGGACCCCATGAAGAATGTCACCTGCGAGAATGGGCTACCGGCCGTGGTGAGT
TGTGTGCCTGGGCAGGTCTTCAGCCCTGACGGACCCTCAAGATTCCGGAAAGCGTACAAG
CCAGAGCAACCCCTGGTGCGACTGAGAGGCGGTGCCTACATCGGGGAGGGCCGCGTGGAG
GTGCTCAAAAATGGAGAATGGGGGACCGTCTGCGACGACAAGTGGGACCTGGTGTCGGCC
AGTGTGGTCTGCAGAGAGCTGGGCTTTGGGAGTGCCAAAGAGGCAGTCACTGGCTCCCGA
CTGGGGCAAGGGATCGGACCCATCCACCTCAACGAGATCCAGTGCACAGGCAATGAGAAG
TCCATTATAGACTGCAAGTTCAATGCCGAGTCTCAGGGCTGCAACCACGAGGAGGATGCT
GGTGTGAGATGCAACACCCCTGCCATGGGCTTGCAGAAGAAGCTGCGCCTGAACGGCGGC
CGCAATCCCTACGAGGGCCGAGTGGAGGTGCTGGTGGAGAGAAACGGGTCCCTTGTGTGG
GGGATGGTGTGTGGCCAAAACTGGGGCATCGTGGAGGCCATGGTGGTCTGCCGCCAGCTG
GGCCTGGGATTCGCCAGCAACGCCTTCCAGGAGACCTGGTATTGGCACGGAGATGTCAAC
AGCAACAAAGTGGTCATGAGTGGAGTGAAGTGCTCGGGAACGGAGCTGTCCCTGGCGCAC
TGCCGCCACGACGGGGAGGACGTGGCCTGCCCCCAGGGCGGAGTGCAGTACGGGGCCGGA
GTTGCCTGCTCAGAAACCGCCCCTGACCTGGTCCTCAATGCGGAGATGGTGCAGCAGACC
ACCTACCTGGAGGACCGGCCCATGTTCATGCTGCAGTGTGCCATGGAGGAGAACTGCCTC
TCGGCCTCAGCCGCGCAGACCGACCCCACCACGGGCTACCGCCGGCTCCTGCGCTTCTCC
TCCCAGATCCACAACAATGGCCAGTCCGACTTCCGGCCCAAGAACGGCCGCCACGCGTGG
ATCTGGCACGACTGTCACAGGCACTACCACAGCATGGAGGTGTTCACCCACTATGACCTG
CTGAACCTCAATGGCACCAAGGTGGCAGAGGGCCACAAGGCCAGCTTCTGCTTGGAGGAC
ACAGAATGTGAAGGAGACATCCAGAAGAATTACGAGTGTGCCAACTTCGGCGATCAGGGC
ATCACCATGGGCTGCTGGGACATGTACCGCCATGACATCGACTGCCAGTGGGTTGACATC
ACTGACGTGCCCCCTGGAGACTACCTGTTCCAGGTTGTTATTAACCCCAACTTCGAGGTT
GCAGAATCCGATTACTCCAACAACATCATGAAATGCAGGAGCCGCTATGACGGCCACCGC
ATCTGGATGTACAACTGCCACATAGGTGGTTCCTTCAGCGAAGAGACGGAAAAAAAGTTT
GAGCACTTCAGCGGGCTCTTAAACAACCAGCTGTCCCCGCAGTAA

Clone variation with respect to NM_002318.2
306 c=>t
>OriGene 5' read for NM_002318 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAAAGAGTAAA
CGCGCGNACTCCGCGCGCGGCTACCTACGCTTGGTGCTTGCTTTCTCCAGCCATTGGAGA
CCAGAGCCGCCCCCTCTGCTCGAGAAAGGGGCTCAGCGGCGGCGGAAGCGGAGGGGGACC
ACCGTGGAGAGCGCGGTCCCAGCCCGGCCACTGCGGATCCCTGAAACCAAAAAGCTCCTG
CTGCTTCTGTACCCCGCCTGTCCCTCCCAGCTGCGCAGGGCCCCTTCGTGGGATCATCAG
CCCGAAGACAGGGATGGAGAGGCCTCTGTGCTCCCACCTCTGCAGCTGCCTGGCTATGCT
GGCCCTCCTGTCCCCCCTGAGCCTGGCACAGTATGACAGCTGGCCCCATTACCCCGAGTA
CTTCCAGCAACCGGCTCCTGAGTATCACCAGCCCCAGGCCCCCGCCAACGTGGCCAAGAT
TCAGCTGCGCCTGGCTGGGCAGAAGAGGAAGCACAGCGAGGGCCGGGTGGAGGTGTACTA
TGATGGCCAGTGGGGCACCGTGTGCGATGACCACTTCTCCATCCACGCTGCCCACGTCGT
CTGCCGGGAGCTGGGCTATGTGGAGGCCAAGTCCTGGACTGCCAGCTCCTCCTACGGCAA
GGGAGAAGGGGCCATCTGGTTAGACAATCTCCACTGTACTGGCAACGAGGCGACCCTTGC
AGCATGCACCTTCAATGGCTGGGGCGTCACTGACTGCAGCACACGGAGGATTGTCGTGTG
GTGTGCCACGACAAAAGGATTCCTGGGNTTAAATTTGCCATTTCGTTGACCACCCAGATA
GAGACCTGGATT
>OriGene 3' read for NM_002318 unedited
CGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAATAAATCTTTCTTTTATTCCAAGT
TTCAAGTATGTGTAAGTGTCTGTGATGACACATGGTGCTCAGTGAGCGCCCTGTCACCCA
GGACCCTGGTTATAGCACCGTTGAATCAAAAACCCACAGAATTGGAACAACAGGAAGATG
AGTCACTTACACAGTGGGTTGGTTTTTCCTTAAATTTGTACTTCCAAGGGCCCAGATGTC
CAATATTAAATGCCAATAAATTTGTGCTTCAAAAGTCTGTGAAAATATAATAATAGTATC
TTGAAAAACAAGGGGTGGGGGTTGTGGAGAGGGAAGCACCAAGTCTGAGCTCACCAAATC
AGAAAACACGTGGCACCATTATCTATGTGACTATAATAGACTGGAGGGTTTCATTGGAAG
AGAGGTCTAGATATTCAAGTTTGCTGAAAATGACCTAAGAGGAGGAGAAATGGGGTTCGG
CCTGACCCTCTCTGGGGGGGCTGATGAGCCCGCATTTGTCCACGTGTCACTGGAGAAGAG
CGCGGCTCCACTGTGTCTGTGGTGAGCTCGGTGGCTTGAATGGGACAAGCTGATGACAAC
CTGTCTGTGGGCCTCATCCCGGTCAAGACTGGCTCTTGGTGCTGCTCCAGCAGCTCTGTG
GACAAACCCCACCCCCCCAAGGCCTTCTCATGCCCCCAAGGGTCAGGTAGCAGCCCTCCA
TGAATGATGGGAGGGTCCCTTTCCTCCTGAGCTTAACACAGCTGTAGGGGTCTGGACAGG
TGGAGGCCAGGCCTGGTGAGGGCACGTGGCATTCGTTCAGACTCAGTTGGTGGNGGGGAA
GTCCCATGGNATATGTGGTGCGGCCCCTGATACAAGATTGACCACCCAGGCTTCTTACTG
AGAGACAGCTGGTTGTTAATAACCCGTGAAAGCTCAAACTTCTTTCGTTCTTGCTGAAGA
CCCCTAGGGCAGTTGACTCAAATGGGGGCGCT
Restriction Sites NotI-NotI     
ACCN NM_002318
Insert Size 3550 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002318.2, NP_002309.1
RefSeq Size 3810 bp
RefSeq ORF 2325 bp
Locus ID 4017
Cytogenetics 8p21.3
Domains SR, Lysyl_oxidase
Protein Families Druggable Genome, Secreted Protein
Gene Summary 'This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]'

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