LOXL2 (NM_002318) Human Untagged Clone

CAT#: SC118675

LOXL2 (untagged)-Human lysyl oxidase-like 2 (LOXL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LOXL2
• Synonyms: LOR; LOR2; WS9-14
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118675 sequence for NM_002318 edited (data generated by NextGen Sequencing)
  ATGGAGAGGCCTCTGTGCTCCCACCTCTGCAGCTGCCTGGCTATGCTGGCCCTCCTGTCC
  CCCCTGAGCCTGGCACAGTATGACAGCTGGCCCCATTACCCCGAGTACTTCCAGCAACCG
  GCTCCTGAGTATCACCAGCCCCAGGCCCCCGCCAACGTGGCCAAGATTCAGCTGCGCCTG
  GCTGGGCAGAAGAGGAAGCACAGCGAGGGCCGGGTGGAGGTGTACTATGATGGCCAGTGG
  GGCACCGTGTGCGATGACGACTTCTCCATCCACGCTGCCCACGTCGTCTGCCGGGAGCTG
  GGCTATGTGGAGGCCAAGTCCTGGACTGCCAGCTCCTCCTACGGCAAGGGAGAAGGGCCC
  ATCTGGTTAGACAATCTCCACTGTACTGGCAACGAGGCGACCCTTGCAGCATGCACCTCC
  AATGGCTGGGGCGTCACTGACTGCAAGCACACGGAGGATGTCGGTGTGGTGTGCAGCGAC
  AAAAGGATTCCTGGGTTCAAATTTGACAATTCGTTGATCAACCAGATAGAGAACCTGAAT
  ATCCAGGTGGAGGACATTCGGATTCGAGCCATCCTCTCAACCTACCGCAAGCGCACCCCA
  GTGATGGAGGGCTACGTGGAGGTGAAGGAGGGCAAGACCTGGAAGCAGATCTGTGACAAG
  CACTGGACGGCCAAGAATTCCCGCGTGGTCTGCGGCATGTTTGGCTTCCCTGGGGAGAGG
  ACATACAATACCAAAGTGTACAAAATGTTTGCCTCACGGAGGAAGCAGCGCTACTGGCCA
  TTCTCCATGGACTGCACCGGCACAGAGGCCCACATCTCCAGCTGCAAGCTGGGCCCCCAG
  GTGTCACTGGACCCCATGAAGAATGTCACCTGCGAGAATGGGCTACCGGCCGTGGTGAGT
  TGTGTGCCTGGGCAGGTCTTCAGCCCTGACGGACCCTCAAGATTCCGGAAAGCGTACAAG
  CCAGAGCAACCCCTGGTGCGACTGAGAGGCGGTGCCTACATCGGGGAGGGCCGCGTGGAG
  GTGCTCAAAAATGGAGAATGGGGGACCGTCTGCGACGACAAGTGGGACCTGGTGTCGGCC
  AGTGTGGTCTGCAGAGAGCTGGGCTTTGGGAGTGCCAAAGAGGCAGTCACTGGCTCCCGA
  CTGGGGCAAGGGATCGGACCCATCCACCTCAACGAGATCCAGTGCACAGGCAATGAGAAG
  TCCATTATAGACTGCAAGTTCAATGCCGAGTCTCAGGGCTGCAACCACGAGGAGGATGCT
  GGTGTGAGATGCAACACCCCTGCCATGGGCTTGCAGAAGAAGCTGCGCCTGAACGGCGGC
  CGCAATCCCTACGAGGGCCGAGTGGAGGTGCTGGTGGAGAGAAACGGGTCCCTTGTGTGG
  GGGATGGTGTGTGGCCAAAACTGGGGCATCGTGGAGGCCATGGTGGTCTGCCGCCAGCTG
  GGCCTGGGATTCGCCAGCAACGCCTTCCAGGAGACCTGGTATTGGCACGGAGATGTCAAC
  AGCAACAAAGTGGTCATGAGTGGAGTGAAGTGCTCGGGAACGGAGCTGTCCCTGGCGCAC
  TGCCGCCACGACGGGGAGGACGTGGCCTGCCCCCAGGGCGGAGTGCAGTACGGGGCCGGA
  GTTGCCTGCTCAGAAACCGCCCCTGACCTGGTCCTCAATGCGGAGATGGTGCAGCAGACC
  ACCTACCTGGAGGACCGGCCCATGTTCATGCTGCAGTGTGCCATGGAGGAGAACTGCCTC
  TCGGCCTCAGCCGCGCAGACCGACCCCACCACGGGCTACCGCCGGCTCCTGCGCTTCTCC
  TCCCAGATCCACAACAATGGCCAGTCCGACTTCCGGCCCAAGAACGGCCGCCACGCGTGG
  ATCTGGCACGACTGTCACAGGCACTACCACAGCATGGAGGTGTTCACCCACTATGACCTG
  CTGAACCTCAATGGCACCAAGGTGGCAGAGGGCCACAAGGCCAGCTTCTGCTTGGAGGAC
  ACAGAATGTGAAGGAGACATCCAGAAGAATTACGAGTGTGCCAACTTCGGCGATCAGGGC
  ATCACCATGGGCTGCTGGGACATGTACCGCCATGACATCGACTGCCAGTGGGTTGACATC
  ACTGACGTGCCCCCTGGAGACTACCTGTTCCAGGTTGTTATTAACCCCAACTTCGAGGTT
  GCAGAATCCGATTACTCCAACAACATCATGAAATGCAGGAGCCGCTATGACGGCCACCGC
  ATCTGGATGTACAACTGCCACATAGGTGGTTCCTTCAGCGAAGAGACGGAAAAAAAGTTT
  GAGCACTTCAGCGGGCTCTTAAACAACCAGCTGTCCCCGCAGTAA
  
  Clone variation with respect to NM_002318.2
  306 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002318 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAAAGAGTAAA
  CGCGCGNACTCCGCGCGCGGCTACCTACGCTTGGTGCTTGCTTTCTCCAGCCATTGGAGA
  CCAGAGCCGCCCCCTCTGCTCGAGAAAGGGGCTCAGCGGCGGCGGAAGCGGAGGGGGACC
  ACCGTGGAGAGCGCGGTCCCAGCCCGGCCACTGCGGATCCCTGAAACCAAAAAGCTCCTG
  CTGCTTCTGTACCCCGCCTGTCCCTCCCAGCTGCGCAGGGCCCCTTCGTGGGATCATCAG
  CCCGAAGACAGGGATGGAGAGGCCTCTGTGCTCCCACCTCTGCAGCTGCCTGGCTATGCT
  GGCCCTCCTGTCCCCCCTGAGCCTGGCACAGTATGACAGCTGGCCCCATTACCCCGAGTA
  CTTCCAGCAACCGGCTCCTGAGTATCACCAGCCCCAGGCCCCCGCCAACGTGGCCAAGAT
  TCAGCTGCGCCTGGCTGGGCAGAAGAGGAAGCACAGCGAGGGCCGGGTGGAGGTGTACTA
  TGATGGCCAGTGGGGCACCGTGTGCGATGACCACTTCTCCATCCACGCTGCCCACGTCGT
  CTGCCGGGAGCTGGGCTATGTGGAGGCCAAGTCCTGGACTGCCAGCTCCTCCTACGGCAA
  GGGAGAAGGGGCCATCTGGTTAGACAATCTCCACTGTACTGGCAACGAGGCGACCCTTGC
  AGCATGCACCTTCAATGGCTGGGGCGTCACTGACTGCAGCACACGGAGGATTGTCGTGTG
  GTGTGCCACGACAAAAGGATTCCTGGGNTTAAATTTGCCATTTCGTTGACCACCCAGATA
  GAGACCTGGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002318 unedited
  CGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAATAAATCTTTCTTTTATTCCAAGT
  TTCAAGTATGTGTAAGTGTCTGTGATGACACATGGTGCTCAGTGAGCGCCCTGTCACCCA
  GGACCCTGGTTATAGCACCGTTGAATCAAAAACCCACAGAATTGGAACAACAGGAAGATG
  AGTCACTTACACAGTGGGTTGGTTTTTCCTTAAATTTGTACTTCCAAGGGCCCAGATGTC
  CAATATTAAATGCCAATAAATTTGTGCTTCAAAAGTCTGTGAAAATATAATAATAGTATC
  TTGAAAAACAAGGGGTGGGGGTTGTGGAGAGGGAAGCACCAAGTCTGAGCTCACCAAATC
  AGAAAACACGTGGCACCATTATCTATGTGACTATAATAGACTGGAGGGTTTCATTGGAAG
  AGAGGTCTAGATATTCAAGTTTGCTGAAAATGACCTAAGAGGAGGAGAAATGGGGTTCGG
  CCTGACCCTCTCTGGGGGGGCTGATGAGCCCGCATTTGTCCACGTGTCACTGGAGAAGAG
  CGCGGCTCCACTGTGTCTGTGGTGAGCTCGGTGGCTTGAATGGGACAAGCTGATGACAAC
  CTGTCTGTGGGCCTCATCCCGGTCAAGACTGGCTCTTGGTGCTGCTCCAGCAGCTCTGTG
  GACAAACCCCACCCCCCCAAGGCCTTCTCATGCCCCCAAGGGTCAGGTAGCAGCCCTCCA
  TGAATGATGGGAGGGTCCCTTTCCTCCTGAGCTTAACACAGCTGTAGGGGTCTGGACAGG
  TGGAGGCCAGGCCTGGTGAGGGCACGTGGCATTCGTTCAGACTCAGTTGGTGGNGGGGAA
  GTCCCATGGNATATGTGGTGCGGCCCCTGATACAAGATTGACCACCCAGGCTTCTTACTG
  AGAGACAGCTGGTTGTTAATAACCCGTGAAAGCTCAAACTTCTTTCGTTCTTGCTGAAGA
  CCCCTAGGGCAGTTGACTCAAATGGGGGCGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002318
• Insert Size: 3550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002318.2, NP_002309.1
• RefSeq Size: 3810 bp
• RefSeq ORF: 2325 bp
• Locus ID: 4017
• Cytogenetics: 8p21.3
• Domains: SR, Lysyl_oxidase
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]'