Lactoferrin (LTF) (NM_002343) Human Untagged Clone

CAT#: SC118685

LTF (untagged)-Human lactotransferrin (LTF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LTF
• Synonyms: GIG12; HEL110; HLF2; LF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118685 sequence for NM_002343 edited (data generated by NextGen Sequencing)
  ATGAAACTTGTCTTCCTCGTCCTGCTGTTCCTCGGGGCCCTCGGACTGTGTCTGGCTGGC
  CGTAGGNNGAGTGTTCAGTGGTGCACCGTATCCCAACCCGAGGCCACAAAATGCTTCCAA
  TGGCAAAGGAATATGAGAAGAGTGCGTGGCCCTCCTGTCAGCTGCATAAAGAGAGACTCC
  CCCATCCAGTGTATCCAGGCCATTGCGGAAAACAGGGCCGATGCTGTGACCCTTGATGGT
  GGTTTCATATACGAGGCAGGCCTGGCCCCCTACAAACTGCGACCTGTAGCGGCGGAAGTC
  TACGGGACCGAAAGACAGCCACGAACTCACTATTATGCCGTGGCTGTGGTGAAGAAGGGC
  GGCAGCTTTCAGCTGAACGAACTGCAAGGTCTGAAGTCCTGCCACACAGGCCTTCGCAGG
  ACCGCTGGATGGAATGTCCCTATAGGGACACTTCGTCCATTCTTGAATTGGACGGGTCCA
  CCTGAGCCCATTGAGGCAGCTGTGGCCAGGTTCTTCTCAGCCAGCTGTGTTCCCGGTGCA
  GATAAAGGACAGTTCCCCAACCTGTGTCGCCTGTGTGCGGGGACAGGGGAAAACAAATGT
  GCCTTCTCCTCCCAGGAACCGTACTTCAGCTACTCTGGTGCCTTCAAGTGTCTGAGAGAC
  GGGGCTGGAGACGTGGCTTTTATCAGAGAGAGCACAGTGTTTGAGGACCTGTCAGACGAG
  GCTGAAAGGGACGAGTATGAGTTACTCTGCCCAGACAACACTCGGAAGCCAGTGGACAAG
  TTCAAAGACTGCCATCTGGCCCGGGTCCCTTCTCATGCCGTTGTGGCACGAAGTGTGAAT
  GGCAAGGAGGATGCCATCTGGAATCTTCTCCGCCAGGCACAGGAAAAGTTTGGAAAGGAC
  AAGTCACCGAAATTCCAGCTCTTTGGCTCCCCTAGTGGGCAGAAAGATCTGCTGTTCAAG
  GACTCTGCCATTGGGTTTTCGAGGGTGCCCCCGAGGATAGATTCTGGGCTGTACCTTGGC
  TCCGGCTACTTCACTGCCATCCAGAACTTGAGGAAAAGTGAGGAGGAAGTGGCTGCCCGG
  CGTGCGCGGGTCGTGTGGTGTGCGGTGGGCGAGCAGGAGCTGCGCAAGTGTAACCAGTGG
  AGTGGCTTGAGCGAAGGCAGCGTGACCTGCTCCTCGGCCTCCACCACAGAGGACTGCATC
  GCCCTGGTGCTGAAAGGAGAAGCTGATGCCATGAGTTTGGATGGAGGATATGTGTACACT
  GCAGGCAAATGTGGTTTGGTGCCTGTCCTGGCAGAGAACTACAAATCCCAACAAAGCAGT
  GACCCTGATCCTAACTGTGTGGATAGACCTGTGGAAGGATATCTTGCTGTGGCGGTGGTT
  AGGAGATCAGACACTAGCCTTACCTGGAACTCTGTGAAAGGCAAGAAGTCCTGCCACACC
  GCCGTGGACAGGACTGCAGGCTGGAATATCCCCATGGGCCTGCTCTTCAACCAGACGGGC
  TCCTGCAAATTTGATGAATATTTCAGTCAAAGCTGTGCCCCTGGGTCTGACCCGAGATCT
  AATCTCTGTGCTCTGTGTATTGGCGACGAGCAGGGTGAGAATAAGTGCGTGCCCAACAGC
  AATGAGAGATACTACGGCTACACTGGGGCTTTCCGGTGCCTGGCTGAGAATGCTGGAGAC
  GTTGCATTTGTGAAAGATGTCACTGTCTTGCAGAACACTGATGGAAATAACAATGAGGCA
  TGGGCTAAGGATTTGAAGCTGGCAGACTTTGCGCTGCTGTGCCTCGATGGCAAACGGAAG
  CCTGTGACTGAGGCTAGAAGCTGCCATCTTGCCATGGCCCCGAATCATGCCGTGGTGTCT
  CGGATGGATAAGGTGGAACGCCTGAAACAGGTGCTGCTCCACCAACAGGCTAAATTTGGG
  AGAAATGGATCTGACTGCCCGGACAAGTTTTGCTTATTCCAGTCTGAAACCAAAAACCTT
  CTGTTCAATGACAACACTGAGTGTCTGGCCAGACTCCATGGCAAAACAACATATGAAAAA
  TATTTGGGACCACAGTATGTCGCAGGCATTACTAATCTGAAAAAGTGCTCAACCTCCCCC
  CTCCTGGAAGCCTGTGAATTCCTCAGGAAGTAA
  
  Clone variation with respect to NM_002343.3
  67 a=>n;68 g=>n;85 g=>a;140 a=>g;1623 c=>t;1894 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002343 unedited
  GTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGAGCAAACCCA
  CCTGCCCTAACTGGCTCCTAGGCACCTTCAAGTCATCTGCTGAAGAAGATAGCAGTCTCA
  CAGGTCAAGGCGATCTTCAAGTAAAGACCCTCTGCTCTGTGTCCTGCCCTCTAGAAGGCA
  CTGAGACCAGAGCTGGGACAGGGCTCAGGGGGCTGCGACTCCTAGGGGCTTGCAGACTAG
  TGGGAGAGAAAGAACATCGCAGCAGCCAGGCAGAACCAGGACAGGTGAGGTGCAGGCTGG
  CTTTCCTCTCGCAGCGCGGTGTGGAGTCCTGTCCTGCCTCAGGGCTTTTCGGAGCCTGGA
  TCCTCAAGGAACAAGTAGACCTGGCCGCGGGGAGTGGGGAGGGAAGGGGTGTCTATTGGG
  CAACAGGGCGGGGCAAAGCCCTGAATAAAGGGGCGCAGGGCAGGCGCAAGTGGCAGAGCC
  TTCGTTTGCCAAGTCGCCTCCAGACCGCAGACATGAAACTTGTCTTCCTCGTCCTGCTGT
  TCCTCGGGGCCCTCGGACTGTGTCTGGCTGGCCGTAGGAGAAGGAGTGTTCAGTGGTGCA
  CCGTATCCCAACCCGAGGCCACAAAATGCTTCCAATGGCAAAGGAATATGAGAAGAGTGC
  GTGGCCCTCCTGTCAGCTGCATAAAGAGAGACTCCCCCATCCAGTGTATCCAGGCCATTG
  CGGAAAACAGGGCCGATGCTGTGACCCTTNGATGGTGGTTTCATATACGAAGCAGGCCTG
  GCCCCCTACAACTGCGACCTGTAGCGGCGGAAGTCTACGGGACCGAAAGACAGCCACGAA
  CTCACTATTATGCCCGTGGNCTGTGGTGAAGAAAGGCNGGCAGCTNTCAGCTGAACGAAC
  TNNGCAGTCTGAAGTCCTGCCACACAGCCTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002343 unedited
  ATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTATCAACAAAATTTCTCATTTTACTTTTTGCTAAAAC
  CACAGCAGGGAATTGTAAGCAAATGGATGGGCAATCCCCACCTTCAGCAGGGGAGGCCAA
  GGCCCCAACACACCTGGGGAAAAAAAGCTGGGGGCAGTGAATGGCTGAGGCTTTTTTGGG
  GAGCTGGGCCATCTTTTTCGGTTTTACTTCCTGAGGAATTCACAGGGCTTTTCCAGAGAG
  GGGGGAGGTTGAGCACTTTTTACAAATTAATAATGCCTGCGACATACTGTGGTGCCCAAA
  AATATTTTTTTATATAATTGGTNNGNCGCCNCCNNCCNCCCCCCCCNTNCCCCCTCCCCC
  CCCCCCCTCTCGTCCCTCGCTCTTCTCCCCCTTCATCTTCTATTCTACCCATTCGTCTTC
  TCCCCATCTCTTCCCCCATCCCTCTCCACCCCCCCTCTTCTTCCCCCCTCCCCCCCCCGC
  TTCTTTTTATCCCTCTTCCTCCCCCCGTATTCCTTTCCACCCCCCCCTTCCCCTCTCCCC
  CTCCTCCCCCTTTTTCCCCCCCTCCCCTCTTCCCTCTCCCCTTTTTTCCCCCCCCTTTTC
  TTCCCCCTTTCCCCCTTCCCCTTTCCTTCTTGTCCCCTCTTCTCTCTCCCGCCTTTCCTT
  CCTCCCTTCCCCATTCTCTTCCCCTCCCTTTCTTTTCCCCTTCCTCTCCTCCTTTCTTTT
  CCCCTTCCCCCTCTCTACCCTATTTCCCCCCCCATTTCTTTTCCCCCCCCTTTTTTATTT
  TCCTTCTCCCCTTCCTCCCCCCTTCCATTTCCTTCCATTCCCTCCCTCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002343
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002343.1, NP_002334.1
• RefSeq Size: 2619 bp
• RefSeq ORF: 2136 bp
• Locus ID: 4057
• Cytogenetics: 3p21.31
• Domains: TR_FER
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).