Lumican (LUM) (NM_002345) Human Untagged Clone

CAT#: SC118686

LUM (untagged)-Human lumican (LUM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LUM
• Synonyms: LDC; SLRR2D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118686 sequence for NM_002345 edited (data generated by NextGen Sequencing)
  ATGAGTCTAAGTGCATTTACTCTCTTCCTGGCATTGATTGGTGGTACCAGTGGCCAGTAC
  TATGATTATGATTTTCCCCTATCAATTTATGGGCAATCATCACCAAACTGTGCACCAGAA
  TGTAACTGCCCTGAAAGCTACCCAAGTGCCATGTACTGTGATGAGCTGAAATTGAAAAGT
  GTACCAATGGTGCCTCCTGGAATCAAGTATCTTTACCTTAGGAATAACCAGATTGACCAT
  ATTGATGAAAAGGCCTTTGAGAATGTAACTGATCTGCAGTGGCTCATTCTAGATCACAAC
  CTTCTAGAAAACTCCAAGATAAAAGGGAGAGTTTTCTCTAAATTGAAACAACTGAAGAAG
  CTGCATATAAACCACAACAACCTGACAGAGTCTGTGGGCCCACTTCCCAAATCTCTGGAG
  GATCTGCAGCTTACTCATAACAAGATCACAAAGCTGGGCTCTTTTGAAGGATTGGTAAAC
  CTGACCTTCATCCATCTCCAGCACAATCGGCTGAAAGAGGATGCTGTTTCAGCTGCTTTT
  AAAGGTCTTAAATCACTCGAATACCTTGACTTGAGCTTCAATCAGATAGCCAGACTGCCT
  TCTGGTCTCCCTGTCTCTCTTCTAACTCTCTACTTAGACAACAATAAGATCAGCAACATC
  CCTGATGAGTATTTCAAGCGTTTTAATGCATTGCAGTATCTGCGTTTATCTCACAACGAA
  CTGGCTGATAGTGGAATACCTGGAAATTCTTTCAATGTGTCATCCCTGGTTGAGCTGGAT
  CTGTCCTATAACAAGCTTAAAAACATACCAACTGTCAATGAAAACCTTGAAAACTATTAC
  CTGGAGGTCAATCAACTTGAGAAGTTTGACATAAAGAGCTTCTGCAAGATCCTGGGGCCA
  TTATCCTACTCCAAGATCAAGCATTTGCGTTTGGATGGCAATCGCATCTCAGAAACCAGT
  CTTCCACCGGATATGTATGAATGTCTACGTGTTGCTAACGAAGTCACTCTTAATTAA
  
  Clone variation with respect to NM_002345.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002345 unedited
  GTATTTGTAAACGACTACTATAGGCGGCCGCGCAATTCGCACGAGGGTTCACAGCATATA
  TTGGTGGATTCTTGTCCATAGTGCATCTGCTTTAAGAATTAACGAAAGCAGTGTCAAGAC
  AGTAAGGATTCAAACCATTTGCCAAAAATGAGTCTAAGTGCATTTACTCTCTTCCTGGCA
  TTGATTGGTGGTACCAGTGGCCAGTACTATGATTATGATTTTCCCCTATCAATTTATGGG
  CAATCATCACCAAACTGTGCACCAGAATGTAACTGCCCTGAAAGCTACCCAAGTGCCATG
  TACTGTGATGAGCTGAAATTGAAAAGTGTACCAATGGTGCCTCCTGGAATCAAGTATCTT
  TACCTTAGGAATAACCAGATTGACCATATTGATGAAAAGGCCTTTGAGAATGTAACTGAT
  CTGCAGTGGCTCATTCTAGATCACAACCTTCTAGAAAACTCCAAGATAAAAGGGAGAGTT
  TTCTCTAAATTGAAACAACTGAAGAAGCTGCATATAAACCACAACAACCTGACAGAGTCT
  GTGGGCCCACTTCCCAAATCTCTGGAGGATCTGCAGCTTACTCATAACAAGATCACAAAG
  CCTGNGCTCTTTCTGAAGGATTGGTAAACCTGACCTTCATCCATCTCCAGCACAATCCGC
  TGAAAGAGGATGCTGTTTCACCTGCTCTTAAAAGTCTTAAATCACTCGAATACCTTGACT
  TGAGCCTCAATCAGATAGCCAGACTGCCTTTTGGTCTCCCTGCCTCTTTTTTAACTCTCT
  ACTTAGACCACAATAAGATCAGCACATCCCTGAAGAGTATTTCAAGCGTTTTAATGCATT
  GCAGGATTCTGCGTTATCTTACAACCAACTGGCTGATAATGGAATACCTGGAAATCTTTC
  AAGGGCCTCCCTGCTGAACTGAACTGTCCTAAACAAGCTATAAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002345 unedited
  ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTGCCCTTTGCTATGGTTTATTTTGCTAGTAGCTTATTAAACATAACATGCCAATA
  ATCAAAGAGAAACCTACCTGACTTAAAGTGAAAAATAATTCTAAAAAAGTTTCACTAGGG
  AAGTATGCAAATTCTTATTCTAAAAATACTTCTTTAAGTGCCTGAAGCTTCATGTATTTT
  GCAATATTCTTGGCCTCAATATCTACCACCTATTTTTTAACCCGACCAATTTGAATGGAT
  CAAGATAATTTGGGGCCAGAAAGTAACATCCCTATGTATTTAATCCAAGCTTTGAGGAAC
  CTTAAGATTTAAGGGTTATAAAACTTGGCTGGTTTCCATGCCACCAGTAAAAGGGTTTCG
  CCATCATTTGCCCGTAGAAATAAAAAAACACTAAATTTACCAATAAAGCACTTGAGTTCG
  AAGGCCATTCCCGGATATGTGGGCCGTCCTGGGACGAAAAAAGCCCGCCCTTCATCTTTT
  CTTTAAAAAAAAAAAAAGGTTACAAAACATTTCCCTTAAATTTTAAAATCCATGGGAAGT
  AATCAACCCGCCATAACAATTGGGTCCCATGAAAACCGCCCCACCGAAAACCCTCCCCCC
  TACATTTTGGTCCCAGCACCCCATTTTTAATAAGAAGGGCCTCCCTCTGCACCACCCTAA
  CATTTCTTCTTATCCGGGGGAAAACCGGTTTTGCAAACCATCGCCTTCCCAACCAATGCT
  TTGTCTGGGGGAGGATAAGGCCCCGCGATCTTGCAAAACCTCTTTTGGACAACTTCCTAA
  CTGATGACCCTCCAGCAAACTTCCACGGGCTCCTTGCACCCTGGCCACCTACAACCTCCC
  CTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002345
• Insert Size: 1970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002345.3, NP_002336.1
• RefSeq Size: 2116 bp
• RefSeq ORF: 1017 bp
• Locus ID: 4060
• Cytogenetics: 12q21.33
• Domains: LRRNT, LRR, LRR_TYP, LRR_SD22, LRR_BAC
• Protein Families: Secreted Protein
• Gene Summary: 'This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]'