Cytokeratin 8 (KRT8) (NM_002273) Human Untagged Clone

CAT#: SC118727

KRT8 (untagged)-Human keratin 8 (KRT8)


  "NM_002273" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol KRT8
Synonyms CARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118727 sequence for NM_002273 edited (data generated by NextGen Sequencing)
ATGTCCATCAGGGTGACCCAGAAGTCCTACAAGGTGTCCACCTCTGGCCCCCGGGCCTTC
AGCAGCCGCTCCTACACGAGTGGGCCCGGTTCCCGCATCAGCTCCTCGAGCTTCTCCCGA
GTGGGCAGCAGCAACTTTCGCGGTGGCCTGGGCGGCGGCTATGGTGGGGCCAGCGGCATG
GGAGGCATCACCGCAGTTACGGTCAACCAGAGCCTGCTGAGCCCCCTTGTCCTGGAGGTG
GACCCCAACATCCAGGCCGTGCGCACCCAGGAGAAGGAGCAGATCAAGACCCTCAACAAC
AAGTTTGCCTCCTTCATAGACAAGGTACGGTTCCTGGAGCAGCAGAACAAGATGCTGGAG
ACCAAGTGGAGCCTCCTGCAGCAGCAGAAGACGGCTCGAAGCAACATGGACAACATGTTC
GAGAGCTACATCAACAACCTTAGGCGGCAGCTGGAGACTCTGGGCCAGGAGAAGCTGAAG
CTGGAGGCGGAGCTTGGCAACATGCAGGGGCTGGTGGAGGACTTCAAGAACAAGTATGAG
GATGAGATCAATAAGCGTACAGAGATGGAGAACGAATTTGTCCTCATCAAGAAGGATGTG
GATGAAGCTTACATGAACAAGGTAGAGCTGGAGTCTCGCCTGGAAGGGCTGACCGACGAG
ATCAACTTCCTCAGGCAGCTGTATGAAGAGGAGATCCGGGAGCTGCAGTCCCAGATCTCG
GACACATCTGTGGTGCTGTCCATGGACAACAGCCGCTCCCTGGACATGGACAGCATCATT
GCTGAGGTCAAGGCACAGTACGAGGATATTGCCAACCGCAGCCGGGCTGAGGCTGAGAGC
ATGTACCAGATCAAGTATGAGGAGCTGCAGAGCCTGGCTGGGAAGCACGGGGATGACCTG
CGGCGCACAAAGACTGAGATCTCTGAGATGAACCGGAACATCAGCCGGCTCCAGGCTGAG
ATTGAGGGCCTCAAAGGCCAGAGGGCTTCCCTGGAGGCCGCCATTGCAGATGCCGAGCAG
CGTGGAGAGCTGGCCATTAAGGATGCCAACGCCAAGTTGTCCGAGCTGGAGGCCGCCCTG
CAGCGGGCCAAGCAGGACATGGCGCGGCAGCTGCGTGAGTACCAGGAGCTGATGAACGTC
AAGCTGGCCCTGGACATCGAGATCGCCACCTACAGGAAGCTGCTGGAGGGCGAGGAGAGC
CGGCTGGAGTCTGGGATGCAGAACATGAGTATTCATACGAAGACCACCAGCGGCTATGCA
GGTGGTCTGAGCTCGGCCTATGGGGGCCTCACAAGCCCCGGCCTCAGCTACAGCCTGGGC
TCCAGCTTTGGCTCTGGCGCGGGCTCCAGCTCCTTCAGCCGCACCAGCTCCTCCAGGGCC
GTGGTTGTGAAGAAGATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTC
CTGCCCAAGTGA

Clone variation with respect to NM_002273.3
681 a=>g
>OriGene 5' read for NM_002273 unedited
ACGGTCACGATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTTCTC
CGCTCCTTCTAGGATCTCCGCCTGGTTCGGCCCGCCTGCCTCCACTCCTGCCTCCACCAT
GTCCATCAGGGTGACCCAGAAGTCCTACAAGGTGTCCACCTCTGGCCCCCGGGCCTTCAG
CAGCCGCTCCTACACGAGTGGGCCCGGTTCCCGCATCAGCTCCTCGAGCTTCTCCCGAGT
GGGCAGCAGCAACTTTCGCGGTGGCCTGGGCGGCGGCTATGGTGGGGCCAGCGGCATGGG
AGGCATCACCGCAGTTACGGTCAACCAGAGCCTGCTGAGCCCCCTTGTCCTGGAGGTGGA
CCCCAACATCCAGGCCGTGCGCACCCAGGAGAAGGAGCAGATCAAGACCCTCAACAACAA
GTTTGCCTCCTTCATAGACAAGGTACGGTTCCTGGAGCAGCAGAACAAGATGCTGGAGAC
CAAGTGGAGCCTCCTGCAGCAGCAGAAGACGGCTCGAAGCAACATGGACAACATGTTCGA
GAGCTACATCAACAACCTTAGGCGGCAGCTGGAGACTCTGGGCCAGGAGAAGCTGAAGCT
GGAGGCGGAGCTTGGCAACATGCAGGGGCTGGTGGAGGACTTCAAGAACAAGTATGAGGA
TGAGATCAATAAGCGTACAGAGATGGAGAACGAATTTGNCCTCATCAAGAAAGATGTGGA
TGAAGCCTACATGAACAAGTAANAGCTGGAGTCTCGCCTGGAAGGCTGACCGACGAGATC
ACTTCCTCCAGCAGCTGTATGAAGAAGAGATCCGGGNAGCTGCAGTCCCAGATCTCGGCA
CATCTGTGGTGCTGTCCATGGACACAGCCGTTCCCTGGCATGGACAGCTCATTGCTTAGG
TAAGCCCAA
>OriGene 3' read for NM_002273 unedited
AGAGTCGAGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTCGACATTGGCAGAGCTAGCTGA
GGTTTTATTTTGGACCAAAAAAAAAAAGCAATTGAATTGTTTTGTACCTGGAGGCATGGG
CAAGGGGGGTCCCCAGGTAGTAAACTCCCCAGGTGGGCTGAGGGCTAAGGCTGAACCTCA
GGTGGGTCTCCTGTTCCCAGTGCTACCCTGCATAGCGGCCTCCTTCCCAGGCTCTGGGGC
AACGCAAGAGGGGTAGGCTGGGAGGGGCTGCCCCAACTGTTCACTTGGGCAGGACGTCAG
AGGACTCAGACACCAGCTTCCCATCACGTGTCTCGATCTTCTTTACAACCACGGCCCTGG
AGGAACTTGTGCGGCTGAAAGAGCTGGACCCCCCGCCAAAGCCAAAGCTGGACCCCAAGC
TGTACCTGAAGCCCGGGCTTTTGAGGCCCCCATAGGCCGAGCTTAGACCACCTGCTTAGC
CCGTGGGGGCATCCACTCTAAAACCTCTGCACCTGCCAACACACTACCAGCGAGCTCCTA
ACACCGACACACCTCCATTTAGGCCGCCTTTTTTTCTTCCCCGCCCCATTTCCCCTTCCC
CCTCTCGCTCCCCTCGTCTCCGCCTTCTCCCCCTTACCATCTCCCCCGTCCCCTCACCCC
GTCTTCCCCCCCCCCGCTTCCCCTCCTTCTTTTTTCCCCTCTTCTCTCTCATTTACCACA
CTCGTTTTCATTCCAATCTTCCGCCGACCACATATGCCAACCCTCATCTACACATTCCCC
TACCGCCCGTCCATGCCCATCTCCACCCCCTCGTCGCCTCCCCTCCTCTTCCCCCTTTCA
CATTCATGTCTTCTCCCGCTCGCGTCATTTCTCCCATTCTATCGCCTTCTCCCACCCCCT
CTATCTTTCCTTTACCCTTGTTTCCTCCCCTCTCTCTGTTTCCGTTTTCTGCCCCTCCTC
CCATACCGCGCTCGCGCTTTGCCGTCGCATATTCCACTGTTTCCCTCCTCCTCCCCATAC
TCTTATACTCCCCCTCACCCTCCTCCT
Restriction Sites NotI-NotI     
ACCN NM_002273
Insert Size 1880 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002273.2, NP_002264.1
RefSeq Size 1788 bp
RefSeq ORF 1452 bp
Locus ID 3856
Cytogenetics 12q13.13
Domains filament
Protein Families Druggable Genome
Gene Summary 'This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]'
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation in a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform 2.

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