Cytokeratin 15 (KRT15) (NM_002275) Human Untagged Clone

CAT#: SC118728

KRT15 (untagged)-Human keratin 15 (KRT15)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT15
• Synonyms: CK15; K1CO; K15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118728 sequence for NM_002275 edited (data generated by NextGen Sequencing)
  ATGACCACCACATTTCTGCAAACTTCTTCCTCCACCTTTGGGGGTGGCTCAACCCGAGGG
  GGTTCCCTCCTGGCTGGGGGAGGTGGCTTTGGTGGGGGGAGTCTCTCTGGGGGAGGTGGA
  AGCCGAAGTATCTCAGCTTCTTCTGCTAGGTTTGTCTCTTCAGGGTCAGGAGGAGGATAT
  GGGGGTGGCATGAGGGTCTGTGGCTTTGGTGGAGGGGCTGGTAGTGTTTTCGGTGGAGGC
  TTTGGAGGGGGCGTTGGTGGGGGTTTTGGTGGTGGCTTTGGTGGTGGCGATGGTGGTCTC
  CTCTCTGGCAATGAGAAAATTACCATGCAGAACCTCAATGACCGCCTGGCCTCCTACCTG
  GACAAGGTACGTGCCCTGGAGGAGGCCAATGCTGACCTGGAGGTGAAGATCCATGACTGG
  TACCAGAAGCAGACCCCAGCCAGCCCAGAATGCGACTACAGCCAATACTTCAAGANCATT
  GAAGAGCTCCGGGACAAGATCATGGCCACCACCATCGACAACTCCCGGGTCATCCTGGAG
  ATCGACAATGCCAGGCTGGCTGCGGACGACTTCAGGCTCAAGTATGAGAATGAGCTGGCC
  CTGCGCCAGGGCGTTGAGGCTGACATCAACGGCTTGCGCCGAGTCCTGGATGAGCTGACC
  CTGGCCAGGACTGACCTGGAGATGCAGATCGAGGGCCTGAATGAGGAGCTAGCCTACCTG
  AAGAAGAACCACGAAGAGGAGATGAAGGAGTTCAGCAGCCAGCTGGCCGGCCAGGTCAAT
  GTGGAGATGGACGCAGCACCGGGTGTGGACCTGACCCGTGTGCTGGCAGAGATGAGGGAG
  CAGTACGAGGCCATGGCGGAGAAGAACCGCCGGGATGTCGAGGCCTGGTTCTTCAGCAAG
  ACTGAGGAGCTGAACAAAGAGGTGGCCTCCAACACAGAAATGATCCAGACCAGCAAGACG
  GAGATCACAGACCTGAGACGCACGATGCAGGAGCTGGAGATCGAGCTGCAGTCCCAGCTC
  AGCATGAAAGCTGGGCTGGAGAACTCACTGGCCGAGACAGAGTGCCGCTATGCCACGCAG
  CTGCAGCAGATCCAGGGGCTCATTGGTGGCCTGGAGGCCCAGCTGAGTGAGCTCCGATGC
  GAGATGGAGGCTCAGAACCAGGAGTACAAGATGCTGCTTGACATAAAGACACGGCTGGAG
  CAGGAGATCGCTACTTACCGCAGCCTGCTCGAGGGCCAGGATGCCAAGATGGCTGGCATT
  GGCATCAGGGAAGCCTCTTCAGGAGGTGGTGGTAGCAGCAGCAATTTCCACATCAATGTA
  GAAGAGTCAGTGGATGGACAGGTGGTTTCTTCCCACAAGAGAGAAATCTAA
  
  Clone variation with respect to NM_002275.3
  439 a=>g;476 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002275 unedited
  CACCAGCTTGGGTTTGCTGAGAACTCACGGGCTCCAGCTACCTGGCCATGACCACCACAT
  TTCTGCAAACTTCTTCCTCCACCTTTGGGGGTGGCTCAACCCGAGGGGGTTCCCTCCTGG
  CTGGGGGAGGTGGCTTTGGTGGGGGGAGTCTCTCTGGGGGAGGTGGAAGCCGAAGTATCT
  CAGCTTCTTCTGCTAGGTTTGTCTCTTCAGGGTCAGGAGGAGGATATGGGGGTGGCATGA
  GGGTCTGTGGCTTTGGTGGAGGGGCTGGTAGTGTTTTCGGTGGAGGCTTTGGAGGGGGCG
  TTGGTGGGGGTTTTGGGTGGTGGCACGTGGTGGTGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002275 unedited
  GCCGCGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTATTTGCAAAAGG
  AAAAGTAATTCTTTATTACATGAACAGACACTGTACAAATGAGCTTGTTACACAATACAG
  CTGCATCTCCTTGCTCCAAAGAAGGTGGGGAGAGGCAGAGGGGGAATAGAGCGCATGCAA
  AGCCCTGAAATAAAAGACCGAGGGACCCTTTTCAGCTCTCTGAAGGCAGGGACTGGAGTT
  TGCATGTGCAGGCCCTCTGGCCAGTCCTCCACTTGGCCTGATGAGAGTGGGGAGTGGCAA
  GGGACGTTTCTCCTGCAATAGACACTTAGATTTCTCTCTTGTGGGAAGAAACCACCTGTC
  CATCCACTGACTCTTCTACATTGATGTGGAAATTGCTGCTGCTACCACCACCTCCTGAAG
  AGGCTTCCCTGATGCCAATGCCAGCCATCTTGGCATCCTGGCCCTCGAGCAGGCTGCGGT
  AAGTAGCGATCTCCTGCTCCAGCCGTGTCTTTATGTCAAGCAGCATCTTGTACTCCTGGT
  TCTGAGCCTCCATCTCGCATCGGAGCTCACTCAGCTGGGCCTCCAGGCCACCAATGAGCC
  CCTGGATCTGCTGCAGCTGCGTGGCATAGCGGCACTCTGTCTCGGCCAGTGAGTTCTCCA
  GCCCAGCTTTCATGCTGAGCTGGGACTGCAGCTCGATCTCCAGCTCCTGCATCGTGCGTC
  TCAAGTCTGTGGATCTCGTCTTGCTGGTCTGGATCATTTCTGGGTTGGAGCCACCTCTTT
  GTTANCTCCTCATCTTGCTGAAGAACAAGCCTNGCATCCCGGGCGTTCTTCTCCGCATTG
  GCTTGTACTGGTCCTTCTTTTTGGCAGCCACCGGTAAGGTCCCACCCGGGGGCTGGGTCC
  ATCTCCACATTGACCTGGCGGCCAACTGGTTGNTGAACCCTTCTCTTCCCTTTGGGGGGC
  TTTCTTAAGGGAGGCTAACCCCTTTTAGGGCCTTCAATTTCATTTCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002275
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002275.2, NP_002266.2
• RefSeq Size: 1709 bp
• RefSeq ORF: 1371 bp
• Locus ID: 3866
• Cytogenetics: 17q21.2
• Domains: filament
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008]'