Lymphocyte Activation Gene 3 (LAG3) (NM_002286) Human Untagged Clone

CAT#: SC118732

LAG3 (untagged)-Human lymphocyte-activation gene 3 (LAG3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LAG3
• Synonyms: CD223
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002286 edited
  CTGCTCTCCGCCACGGCCCTGCTCTGTTCCCTGGGACACCCCCGCCCCCACCTCCTCAGG
  CTGCCTGATCTGCCCAGCTTTCCAGCTTTCCTCTGGATTCCGGCCTCTGGTCATCCCTCC
  CCACCCTCTCTCCAAGGCCCTCTCCTGGTCTCCCTTCTTCTAGAACCCCTTCCTCCACCT
  CCCTCTCTGCAGAACTTCTCCTTTACCCCCCACCCCCCACCACTGCCCCCTTTCCTTTTC
  TGACCTCCTTTTGGAGGGCTCAGCGCTGCCCAGAC:CATAG:GAGAGATGTGGGAGGCTC
  AGTTCCTGGGCTTGCTGTTTCTGCAGCCGCTTTGGGTGGCTCCAGTGAAGCCTCTCCAGC
  CAGGGGCTGAGGTCCCGGTGGTGTGGGCCCAGGAGGGGGCTCCTGCCCAGCTCCCCTGCA
  GCCCCACAATCCCCCTCCAGGATCTCAGCCTTCTGCGAAGAGCAGGGGTCACTTGGCAGC
  ATCAGCCAGACAGTGGCCCGCCCGCTGCCGCCCCCGGCCATCCCCTGGCCCCCGGCCCTC
  ACCCGGCGGCGCCCTCCTCCTGGGGGCCCAGGCCCCGCCGCTACACGGTGCTGAGCGTGG
  GTCCCGGAGGCCTGCGCAGCGGGAGGCTGCCCCTGCAGCCCCGCGTCCAGCTGGATGAGC
  GCGGCCGGCAGCGCGGGGACTTCTCGCTATGGCTGCGCCCAGCCCGGCGCGCGGACGCCG
  GCGAGTACCGCGCCGCGGTGCACCTCAGGGACCGCGCCCTCTCCTGCCGCCTCCGTCTGC
  GCCTGGGCCAGGCCTCGATGACTGCCAGCCCCCCAGGATCTCTCAGAGCCTCCGACTGGG
  TCATTTTGAACTGCTCCTTCAGCCGCCCTGACCGCCCAGCCTCTGTGCATTGGTTCCGGA
  ACCGGGGCCAGGGCCGAGTCCCTGTCCGGGAGTCCCCCCATCACCACTTAGCGGAAAGCT
  TCCTCTTCCTGCCCCAAGTCAGCCCCATGGACTCTGGGCCCTGGGGCTGCATCCTCACCT
  ACAGAGATGGCTTCAACGTCTCCATCATGTATAACCTCACTGTTCTGGGTCTGGAGCCCC
  CAACTCCCTTGACAGTGTACGCTGGAGCAGGTTCCAGGGTGGGGCTGCCCTGCCGCCTGC
  CTGCTGGTGTGGGGACCCGGTCTTTCCTCACTGCCAAGTGGACTCCTCCTGGGGGAGGCC
  CTGACCTCCTGGTGACTGGAGACAATGGCGACTTTACCCTTCGACTAGAGGATGTGAGCC
  AGGCCCAGGCTGGGACCTACACCTGCCATATCCATCTGCAGGAACAGCAGCTCAATGCCA
  CTGTCACATTGGCAATCATCACAGTGACTCCCAAATCCTTTGGGTCACCTGGATCCCTGG
  GGAAGCTGCTTTGTGAGGTGACTCCAGTATCTGGACAAGAACGCTTTGTGTGGAGCTCTC
  TGGACACCCCATCCCAGAGGAGTTTCTCAGGACCTTGGCTGGAGGCACAGGAGGCCCAGC
  TCCTTTCCCAGCCTTGGCAATGCCAGCTGTACCAGGGGGAGAGGCTTCTTGGAGCAGCAG
  TGTACTTCACAGAGCTGTCTAGCCCAGGTGCCCAACGCTCTGGGAGAGCCCCAGGTGCCC
  TCCCAGCAGGCCACCTCCTGCTGTTTCTCATCCTTGGTGTCCTTTCTCTGCTCCTTTTGG
  TGACTGGAGCCTTTGGCTTTCACCTTTGGAGAAGACAGTGGCGACCAAGACGATTTTCTG
  CCTTAGAGCAAGGGATTCACCCTCCGCAGGCTCAGAGCAAGATAGAGGAGCTGGAGCAAG
  AACCGGAGCCGGAGCCGGAGCCGGAACCGGAGCCCGAGCCCGAGCCCGAGCCGGAGCAGC
  TCTGACCTGGAGCTGAGGCAGCCAGCAGATCTCAGCAGCCCAGTCCAAATAAACTCCCTG
  TCAGCAGCAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002286 unedited
  AATTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCTCTCCGCC
  ACGGCCCTGCTCTGTTCCCTGGGACACCCCCGCCCCCACCTCCTCAGGCTGCCTGATCTG
  CCCAGCTTTCCAGCTTTCCTCTGGATTCCGGCCTCTGGTCATCCCTCCCCACCCTCTCTC
  CAAGGCCCTCTCCTGGTCTCCCTTCTTCTAGAACCCCTTCCTCCACCTCCCTCTCTGCAG
  AACTTCTCCTTTACCCCCCACCCCCCACCACTGCCCCCTTTCCTTTTCTGACCTCCTTTT
  GGAGGGCTCAGCGCTGCCCAGACCATAGGAGAGATGTGGGAGGCTCAGTTCCTGGGCTTG
  CTGTTTCTGCAGCCGCTTTGGGTGGCTCCAGTGAAGCCTCTCCAGCCAGGGGCTGAGGTC
  CCGGTGGTGTGGGCCCAGGAGGGGGCTCCTGCCCAGCTCCCCTGCAGCCCCACAATCCCC
  CTCCAGGATCTCAGCCTTCTGCGAAGAGCAGGGGTCACTTGGCAGCATCAGCCAGACAGT
  GGCCCGCCCGCTGCCGCCCCCGGCCATCCCCTGGCCCCCGGCCCTCACCCGGCGGCGCCC
  TCCTCCTGGGGGCCCAGGCCCCGCCGCTACACGGTGCTGAGCGTGGGTCCCGGAGGCCTG
  CGCAGNCGGAGGCTGCCCCTGCAGCCCCGCGTCCAGCTGGATGAGCCGCGCCCCGCAGCG
  CGGGACTTCTCGCTATGGCTGCGCCCAGCCCCGCGCGCGGACGCCNGCGAGTACCGCGCC
  CGCGTGCACCTNAGGNNACCGNGCCCTCTCCTGCCGNCTNCGTNTGCGCCTGGGGCCAGC
  CTCGATGACTGCCAGNCCCCCAGATCTCTCAGACCTCCGACTGGGTCATTNTGACTGCTC
  CTTAGCGCCTGACGCCCANCCTGTGCATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002286 unedited
  CCGCGGCCGCATTTTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTGCTGACAGGGAGTTT
  ATTTGGACTGGGCTGCTGAGATCTGCTGGCTGCCTCAGCTCCAGGTCAGAGCTGCTCCGG
  CTCGGGCTCGGGCTCGGGCTCCGGTTCCGGCTCCGGCTCCGGCTCCGGTTCTTGCTCCAG
  CTCCTCTATCTTGCTCTGAGCCTGCGGAGGGTGAATCCCTTGCTCTAAGGCAGAAAATCG
  TCTTGGTCGCCACTGTCTTCTCCAAAGGTGAAAGCCAAAGGCTCCAGTCACCAAAAGGAG
  CAGAGAAAGGACACCAAGGATGAGAAACAGCAGGAGGTGGCCTGCTGGGAGGGCACCTGG
  GGCTCTCCCAGAGCGTTGGGCACCTGGGCTAGACAGCTCTGTGAAGTACACTGCTGCTCC
  AAGAAGCCTCTCCCCCTGGTACAGCTGGCATTGCCAAGGCTGGGAAAGGAGCTGGGCCTC
  CTGTGCCTCCAGCCAAGGTCCTGAGAAACTCCTCTGGGATGGGGTGTCCAGAGAGCTCCA
  CACAAAGCGTTCTTGTCCAGATACTGGAGTCACCTCACAAAGCAGCTTCCCCAGGGATCC
  ANGTGACCCAAAGGATTTGGGAGTCACTGTGATGATTGCCAATGTGACAGTGGCACTGAG
  CTGCTGTTCCTGCAGATGGATATGGCACGCGTAAGTCCCACCCTGGGCCTGCCTCACATC
  CTCTAGTCGAAGGGTAAAGTCGCCATTGTCTTCATCACCAGGAGGTCACGGCCTCCCCCA
  GNAGGAGTCCCCTTGGCAGTGAAGAAAGACCTGGCTCCCCCCCCCAACAGGCAGCGGCAG
  GCCAACCCCACCCTGNACCCTGCTCACCGTACACTGNCCAGGGAGTTGGGGGCTCCAGAC
  CCCCACACGGGGTTTCCCTGAGGAAACCTGGAGCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002286
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002286.4, NP_002277.3
• RefSeq Size: 1991 bp
• RefSeq ORF: 1578 bp
• Locus ID: 3902
• Cytogenetics: 12p13.31
• Domains: ig, IG
• Protein Families: Transmembrane
• Gene Summary: 'Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]'