Kir4.1 (KCNJ10) (NM_002241) Human Untagged Clone

CAT#: SC118741

KCNJ10 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ10
• Synonyms: BIRK-10; KCNJ13-PEN; KIR1.2; KIR4.1; SESAME
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118741 sequence for NM_002241 edited (data generated by NextGen Sequencing)
  ATGACGTCAGTTGCCAAGGTGTATTACAGTCAGACCACTCAGACAGAAAGCCGGCCCCTA
  ATGGGCCCAGGGATACGACGGCGGAGAGTCCTGACAAAAGATGGTCGCAGCAACGTGAGA
  ATGGAGCACATTGCCGACAAGCGCTTCCTCTACCTCAAGGACCTGTGGACAACCTTCATT
  GACATGCAGTGGCGCTACAAGCTTCTGCTCTTCTCTGCGACCTTTGCAGGCACATGGTTC
  CTCTTTGGCGTGGTGTGGTATCTGGTAGCTGTGGCACATGGGGACCTGCTGGAGCTGGAC
  CCCCCGGCCAACCACACCCCCTGTGTGGTACAGGTGCACACACTCACTGGAGCCTTCCTC
  TTCTCCCTTGAATCCCAAACCACCATTGGCTATGGCTTCCGCTACATCAGTGAGGAATGT
  CCACTGGCCATTGTGCTTCTTATTGCCCAGCTGGTGCTCACCACCATCCTGGAAATCTTC
  ATCACAGGTACCTTCCTGGCGAAGATTGCCCGGCCCAAGAAGCGGGCTGAGACCATTCGT
  TTCAGCCAGCATGCAGTTGTGGCCTCCCACAATGGCAAGCCCTGCCTCATGATCCGAGTT
  GCCAATATGCGCAAAAGCCTCCTCATTGGCTGCCAGGTGACAGGAAAACTGCTTCAGACC
  CACCAAACCAAGGAAGGGGAGAACATCCGGCTCAACCAGGTCAATGTGACTTTCCAAGTA
  GACACAGCCTCTGACAGCCCCTTCCTTATTCTACCCCTTACCTTCTATCATGTGGTAGAT
  GAGACCAGTCCCTTGAAAGATCTCCCTCTTCGCAGTGGTGAGGGTGACTTTGAGCTGGTG
  CTGATCCTAAGTGGGACAGTGGAGTCCACCAGTGCCACCTGTCAGGTGCGCACTTCCTAC
  CTGCCAGAGGAGATCCTTTGGGGCTACGAGTTCACACCTGCCATCTCACTGTCAGCCAGT
  GGTAAATACATAGCTGACTTTAGCCTTTTTGACCAAGTTGTGAAAGTGGCCTCTCCTAGT
  GGCCTCCGTGACAGCACTGTACGCTACGGAGACCCTGAAAAGCTCAAGTTGGAGGAGTCA
  TTAAGGGAGCAAGCTGAGAAGGAGGGCAGTGCCCTTAGTGTGCGCATCAGCAATGTCTGA
  
  Clone variation with respect to NM_002241.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002241 unedited
  ACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGGCACAGCTCTGCTCCTAACTCC
  TGCCCGCCCCGTCCGTCCATCTGTCCCGCTGCCCCGCGGCCCATCCAAGGGGCCACTCCA
  CCTCGGACCCAAGATGACGTCAGTTGCCAAGGTGTATTACAGTCAGACCACTCAGACAGA
  AAGCCGGCCCCTAATGGGCCCAGGGATACGACGGCGGAGAGTCCTGACAAAAGATGGTCG
  CAGCAACGTGAGAATGGAGCACATTGCCGACAAGCGCTTCCTCTACCTCAAGGACCTGTG
  GACAACCTTCATTGACATGCAGTGGCGCTACAAGCTTCTGCTCTTCTCTGCGACCTTTGC
  AGGCACATGGTTCCTCTTTGGCGTGGTGTGGTATCTGGTAACTGTGGCACATGGGGACCT
  GCTGGAGCTGGACCCCCCGGGCAACCACACCCCCTGTGTGGTACAGGTGCACACACTCAC
  TGGAGCCTTGCTCTTGTGCCTTGAATCCAAACCACCATTGGGTATGGGTTACGGTACATA
  AGTGAGGAATGTGCACTGGACATTGTGCTTGTTATTGAGCAGGTGGTGCTGGCCACCATG
  CTGAGAATCTAGATGCAAGATCCTTGCTGGGGAAAATTGACCGGACGAAAACGGGAGTGA
  AACCGTGGTTTGGAAGCGCGTGCGGAGTGGGGACTGTGGCAATGGGTGATGCGTGCGTGT
  GAAGCAGGTGATGTACTTGGCGGAGACCGGGCTAGGTTGTTGGGTTGATGGACNGATGAA
  CTATGGTGGTGCGTGGGCCAGGGGTGAGGGGTGGACCCTGTGCTGGAGCCGCGCGCTTGT
  GAGTGTGTGGGTGGCCCCCGCGGCGAGAGGGGCGGGCTTAAGACCAGGCCAGGAGGNNTG
  GTGGGGGGNAGCGGGACGGATATAAGCTCGTTACACAACTATGGATAAAAGAACGATATA
  AAAGATAAGACTAANATATTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002241 unedited
  GTACCGCGGCACGCATTTCTAGTAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTAGG
  AAACAAAAGGGGCTTTATTTTATTTTATTTATTTTAAAAAGGCGCTACAGTGGCAGGACG
  CTGCTGGGGATGGGGACACACAGTGTAAGTGGGAGATTTTCCAAGACTTCCCCTCCCTAT
  TGGCCTCCGAGTTCCTCTCTTCCTGGGCCTATCAGTAGCATAGGATTCAACAAAAGCCTT
  CATGAATGCCTCTTTATTTCTGAAAAGGAACTTCTACCCCTCCCTTTCCCATTTCCAGAC
  TTGAAATCCTTCATCAGCTATCTCACTTCCATGTTGAAACCAGGACCACTGATTGTAAGT
  CTGAAAATCTGGGTGTCCTACCCCATAACCAGGCCAAGATATCTGCCCCCAATGCTAAAG
  GCTCTGAATATTTCCTTTCTGGACATTCAATATATTTGGGCATATATACATCTATATACA
  TCCTTCTATATATATATTATCTCTGGTTCTTTCTTCTTAGATAAACCCTTCATATATGGC
  ATATCCTAATTTCCCAAGCCCTCCCACTCTTTGTTTGCGAGCCTAAGCAAGACTCAAGAG
  TTTCATTGATTTCACTAAGGTCCCCAGCATCATTGCTTTCTCATCTTTCTACCCCAAGAT
  TCTTCCCCATCACGTGTTTCCCCCCCTTCCGAAAACCCCCTTTTTAACACCGTGCCTACC
  GAAATTCCCCTTTTTAAACTGGGCCTCTACTCTGCTGATTTCCAAGCCCCAGTGGGTTGG
  GGGCCCATTTCTAACAAACCTGGCGGGTTCTAAGCCGGCCTATGGCTAAAGAAGATTAGG
  GCCAAGGGAAAAACATTGTCCAAAAACCCTTTAAGTAAGGAAGGGGGACTTGCCCCCGCG
  CAGGGCCTCGGGGAAAAAACCCATCCCCTTTCAGGACACGGGTTTAAGTGCCCCTTAACG
  AACCCGGGGAAACCGCAGGATCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002241
• Insert Size: 4600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002241.2, NP_002232.2
• RefSeq Size: 4515 bp
• RefSeq ORF: 1140 bp
• Locus ID: 3766
• Cytogenetics: 1q23.2
• Domains: IRK
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]'