IRF1 (NM_002198) Human Untagged Clone

CAT#: SC118744

IRF1 (untagged)-Human interferon regulatory factor 1 (IRF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRF1
• Synonyms: IRF-1; MAR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002198, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCATCACTCGGATGCGCATGAGACCCTGGCTAGAGATGCAGATTAATTCCAACCAAATCCCGGGGC
  TCATCTGGATTAATAAAGAGGAGATGATCTTCCAGATCCCATGGAAGCATGCTGCCAAGCATGGCTGGGA
  CATCAACAAGGATGCCTGTTTGTTCCGGAGCTGGGCCATTCACACAGGCCGATACAAAGCAGGGGAAAAG
  GAGCCAGATCCCAAGACGTGGAAGGCCAACTTTCGCTGTGCCATGAACTCCCTGCCAGATATCGAGGAGG
  TGAAAGACCAGAGCAGGAACAAGGGCAGCTCAGCTGTGCGAGTGTACCGGATGCTTCCACCTCTCACCAA
  GAACCAGAGAAAAGAAAGAAAGTCGAAGTCCAGCCGAGATGCTAAGAGCAAGGCCAAGAGGAAGTCATGT
  GGGGATTCCAGCCCTGATACCTTCTCTGATGGACTCAGCAGCTCCACTCTGCCTGATGACCACAGCAGCT
  ACACAGTTCCAGGCTACATGCAGGACTTGGAGGTGGAGCAGGCCCTGACTCCAGCACTGTCGCCATGTGC
  TGTCAGCAGCACTCTCCCCGACTGGCACATCCCAGTGGAAGTTGTGCCGGACAGCACCAGTGATCTGTAC
  AACTTCCAGGTGTCACCCATGCCCTCCACCTCTGAAGCTACAACAGATGAGGATGAGGAAGGGAAATTAC
  CTGAGGACATCATGAAGCTCTTGGAGCAGTCGGAGTGGCAGCCAACAAACGTGGATGGGAAGGGGTACCT
  ACTCAATGAACCTGGAGTCCAGCCCACCTCTGTCTATGGAGACTTTAGCTGTAAGGAGGAGCCAGAAATT
  GACAGCCCAGGGGGGGATATTGGGCTGAGTCTACAGCGTGTCTTCACAGATCTGAAGAACATGGATGCCA
  CCTGGCTGGACAGCCTGCTGACCCCAGTCCGGTTGCCCTCCATCCAGGCCATTCCCTGTGCACCGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002198 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTAGTCGAGGCA
  AGACGTGCGCCCGAGCCCCGCCGAACCGAGGCCACCCGGAGCCGTGCCCAGTCCACGCCG
  GCCGTGCCCGGCGGCCTTAAGAACCCGGCAACCTCTGCCTTCTTCCCTCTTCCACTCGGA
  GTCGCGCTTCGCGCGCCCTCACTGCAGCCCCTGCGTCGCCGGGACCCTCGCGCGCGACCG
  CCGAATCGCTCCTGCAGCAGAGCCAACATGCCCATCACTCGGATGCGCATGAGACCCTGG
  CTAGAGATGCAGATTAATTCCAACCAAATCCCGGGGCTCATCTGGATTAATAAAGAGGAG
  ATGATCTTCCAGATCCCATGGAAGCATGCTGCCAAGCATGGCTGGGACATCAACAAGGAT
  GCCTGTTTGTTCCGGAGCTGGGCCATTCACACAGGCCGATACAAAGCAGGGGAAAAGGAG
  CCAGATCCCAAGACGTGGAAGGCCAACTTTCGCTGTGCCATGAACTCCCTGCCAGATATC
  GAGGAGGTGAAAGACCAGAGCAGGAACAAGGGCAGCTCAGCTGTGCGAGTGTACCGGATG
  CTTCCACCTCTCACCAAGAACCAGAGAAAAGAAAGAAGGTTCGAAGTCCAGCCGAGATGC
  TAAGAGCAAGGCCAGNNAGGAAGTCATGTGGGGGGATTCCAGCCCTGATACCTTCTCTGA
  TGGACTCAGCAGCTCCACTCTGCCTGATGACCACAGCACCTACACAGTTCCAGGCTCATG
  CAGACTTTGGGAGGGGGGAACAGGCCCTGACTCACCTGGNNNCCCCGGGTGCTGTCACAG
  CACTCTCCCCGACTGCACATCCAAANGGGAAAGGTGGCGCGGACAGACCCCGGGATCTGT
  CAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002198 unedited
  GGCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTGGGGCCCGGGCTGGAGT
  GCAATGGCATGTTCTCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGCGATTCTCTTG
  CCTCAGCCTCCTGAGTAGCTGGGATTACAAGCACCCGCAACCACGCCCAGCTAATTTTTG
  CATTTTTAGTAAAAATGGAGTTTCACCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATC
  CACCCATCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCGCCTGTCCGA
  AAGGATTTTATAACAGATGCATGTCCCTGTTCACCCCAAAGTCAAGTTCAAGCGGGATGC
  CAATAATAAGTCCATCAGTGTTTCCATAAAAAGTAAAATCATCTACAAGGCAAATTTCTC
  TCTAAAAAGGCCACACCCTTATAAACAATTTACTTTTTTATAAAAAGACAAATGTACATA
  TTTACACACGTCCTTATGTGTTCACACATGACTTAAAGGCTAAATGACATTTACAATTTT
  AAGTTATACCAAGGCGCCTCACACTTACCTCCCTGGGCCCTGTTGAAGGCCTGCTGGGGC
  TTGAAGGGGCGGTGGGCCCCTAGCCACGCCCTGTTTAAATAAACGCAAAGGCCAAAATCT
  CGGTGGAAAAGTTAATGCTCACCCCTGATCAAAATGTGGCTTGATCCCACCAAAAATTCC
  AATTGTTTTTCCCACTCCCCCCCCCATCAGGAGCCCAGGACACCCCTCTGAGGCTGAGGA
  ACCCTGCCCAGAAGAACCCCTTTTCCTCAATATACCGCGTTTGGAACACACCCCCGGCTA
  TTTTAAATGGGGTTATTTTTTGCAACCAAATATATATCGCCATTTTTTTACCGCATACCC
  ACCTGGATCGTGCCTTACACCCTGCGCCCCCGCCCGATCCCAGTCACATCCTTTTCNTTA
  TACCCCTTCACACCGGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002198
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002198.1, NP_002189.1
• RefSeq Size: 2035 bp
• RefSeq ORF: 978 bp
• Locus ID: 3659
• Cytogenetics: 5q31.1
• Domains: IRF
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses. The encoded protein activates the transcription of genes involved in the body's response to viruses and bacteria, playing a role in cell proliferation, apoptosis, the immune response, and DNA damage response. This protein represses the transcription of several other genes. As a tumor suppressor, it both suppresses tumor cell growth and stimulates an immune response against tumor cells. Defects in this gene have been associated with gastric cancer, myelogenous leukemia, and lung cancer. [provided by RefSeq, Aug 2017]'