GIRK1 (KCNJ3) (NM_002239) Human Untagged Clone

CAT#: SC118769

KCNJ3 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ3
• Synonyms: GIRK1; KGA; KIR3.1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118769 sequence for NM_002239 edited (data generated by NextGen Sequencing)
  ATGTCTGCACTCCGAAGGAAATTTGGGGACGATTATCAGGTAGTGACCACATCGTCCAGC
  GGCTCGGGCTTGCAGCCCCAGGGGCCAGGCCAGGACCCTCAGCAGCAGCTTGTGCCCAAG
  AAGAAGCGGCAGCGGTTCGTGGACAAGAACGGCCGGTGCAATGTACAGCACGGCAACCTG
  GGCAGCGAGACAAGCCGCTACCTCTCGGACCTCTTCACCACGCTGGTGGACCTCAAGTGG
  CGCTGGAACCTCTTCATCTTCATTCTCACCTACACCGTGGCCTGGCTTTTCATGGCGTCC
  ATGTGGTGGGTGATCGCCTACACTCGGGGCGACCTGAACAAAGCCCACGTCGGTAACTAC
  ACGCCTTGCGTGGCCAATGTCTATAACTTCCCTTCTGCCTTCCTCTTCTTCATCGAGACG
  GAGGCCACCATCGGCTATGGCTACCGATACATCACAGACAAGTGCCCCGAGGGCATCATC
  CTCTTCCTCTTCCAGTCCATCCTGGGCTCCATCGTGGACGCCTTCCTCATCGGCTGCATG
  TTCATCAAGATGTCCCAGCCCAAGAAGCGCGCCGAGACCCTCATGTTCAGCGAGCACGCG
  GTGATCTCCATGAGGGACGGAAAACTCACGCTTATGTTCCGGGTGGGCAACCTGCGCAAC
  AGCCACATGGTCTCCGCGCAGATTCGCTGCAAGCTGCTCAAATCTCGGCAGACACCTGAG
  GGTGAGTTCCTTCCCCTTGACCAACTTGAACTGGATGTAGGTTTTAGTACAGGGGCAGAT
  CAACTTTTTCTTGTGTCCCCCCTCACAATTTGCCACGTGATCGATGCCAAAAGCCCCTTT
  TATGACCTATCCCAGCGAAGCATGCAAACTGAACAGTTCGAGATTGTCGTCATCCTAGAA
  GGCATTGTGGAAACAACTGGGATGACTTGTCAAGCTCGAACATCATATACTGAAGATGAA
  GTTCTTTGGGGTCATCGTTTTTTTCCTGTAATTTCCTTAGAAGAGGGATTCTTTAAAGTT
  GATTACTCCCAGTTCCATGCAACATTTGAAGTCCCCACCCCACCTTACAGTGTGAAAGAG
  CAGGAGGAAATGCTTCTCATGTCGTCCCCTTTAATAGCACCAGCCATAACTAACAGCAAA
  GAAAGACATAATTCTGTGGAATGCTTAGATGGACTAGATGATATTACTACAAAACTACCA
  TCTAAGCTGCAGAAAATTACTGGAAGAGAAGACTTTCCCAAAAAACTCTTGAGGATGAGT
  TCTACAACTTCAGAAAAAGCCTACAGCTTGGGAGACTTGCCCATGAAACTTCAACGAATA
  AGTTCAGTTCCGGGCAACTCAGAAGAAAAACTGGTATCTAAAACCACCAAGATGTTATCT
  GATCCCATGAGCCAGTCTGTGGCTGATTTGCCACCAAAGCTTCAAAAGATGGCTGGAGGA
  GCAGCTAGGATGGAAGGGAACCTTCCAGCCAAATTAAGAAAAATGAACTCTGATCGCTTC
  ACATAA
  
  Clone variation with respect to NM_002239.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002239 unedited
  GCATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGTGACCCGGGATGC
  CGGTGGTGGGGAAAGAAAAGAGAGCCTCTGGAAGCTTGGAGGCAAAATTGCGCTTGGGTT
  CCTGTTCCTTGCATCCCTCCTGGCTTGAGTGCGGGAGAACACTTTTTAAAGACTCACCTT
  GGAAAGAAGGCCTCCGTCCCAGGGGAGAAGGAGAGGCGTCTGCAGGGGGCAGAGACCGCA
  GCTACCTGCCGGGTGCGCCCCCCACCCAGGAGCGCTCGCTTCGCCCCCTTTCCTCCCCCG
  CCCCCACCTCCTTATTGGTGCTAGTTTGCAGCGCCCAGCTCCTGCGCCTTCGCTTCGCGT
  TTGAATCTGGCTCGCCCCTTCGTATTATGTCTGCACTCCGAAGGAAATTTGGGGACGATT
  ATCAGGTAGTGACCACATCGTCCAGCGGCTCGGGCTTGCAGCCCCAGGGGCCAGGCCAGG
  ACCCTCAGCAGCAGCTTGTGCCCAAGAAGAAGCGGCAGCGGTTCGTGGACAAGAACGGCC
  GGTGCAATGTACAGCACGGCAACCTGGGCAGCGAGACAAGCCGCTACCTCTCGGACCTCT
  TCACCACGCTGGTGGACCTCAAGTGGCGCTGGAACCTCTTCATCTTCATTCTCACCTACA
  CCGTGGCCTGGCTTTTCATGGCGTCCATGTGGTGGGGTGATCGCCTACACTCGGNGCGAC
  CTGAACAAGCCCACGTCGGTAACTACACGCCTTGCGTGGCCAATGTCTATAACTTCCTTN
  CTGCCTTCCTCTTCTTCATCGAGACGGAGGCCACCATCGGCTATGGCTACCGATACATCA
  CAGACAAGTGCCCCCGAGGCATCATNCTCTTCCTCTTNCAGNCCATNCTGGGCTCCATCG
  GGGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002239 unedited
  CTCCCCACCCCCCCCACCCACCTTTTTCCCTCCTTACACACAATTTGACTTTGNACCGCG
  GNCCGCATACTAGNGATCGGTTTTTTTTTTTTTTTTTTGAAATGTTTCCAATTTACTCAG
  TTTGGAACAGGGACAATTAATTGTGTAACTTCACACAGATTCTGATATATTCAATATAAC
  TTGGCTACTGTCTATTAACATGATTCAGTGTTTGTTCAGAGCTCATCTTTGTATTAGATC
  ATATACAAAACAGATTTCCAGATTTTAATTAGTCTAGGAAAGGAATAATTCTCAAATTAA
  TCAACAATTATTTCTCCCCAGTACTAGCAACTTTTAAAAATCATAAGAAATGTTCAATGC
  TATACTGAGATCTGGTATGCTCTAAAATTTACAATTTAAACATGTAATGGTTATATTTTT
  GCAGGGCCATAGATAGCACAAACTTGTGTGATTCTCTCATATAAAATACAATTGAAAGCC
  TCCCAAGACCTTAAACAGCAGAAAACATTAAATCACATTTTAAAATGACAAAAATTTAAG
  GCTAAAATATTTGGTATTAATAATTAATGCCAGTAGCCTTCCTACTCTAATAAAGTAAAC
  ATGCACAAGAACTACAACTTTGAACAAACCTCACAATTTTTATCAGATATATATGCATGT
  ATGTATGACTGCGGATGTATGTGCATATATATATGCAAAAGGCCGGGCCACCTTACCCCC
  CCCCCCATCCCCCCTCCTTTTCATCCCCCTCGTCCTGCCACATTACTCCCCTCTGTCCCG
  GGGTTACTACCATTTCGTCAGAACGCTCCCCCTTGACGCTCCTATCTTTCACACTCTTAC
  AATTCCATTTTCATATTCCCTAACTTTCCCTGTCCCCTTTCTCTCCTCCCCGCCCCCCTG
  TCCTCATTCTCAAAGGCGTCACCTTCCCCCTTCATCCTCCTCCTCTACGTCCCACCCCTC
  GCCACCATCCTCAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002239
• Insert Size: 2870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002239.2, NP_002230.1
• RefSeq Size: 2890 bp
• RefSeq ORF: 1506 bp
• Locus ID: 3760
• Cytogenetics: 2q24.1
• Domains: IRK
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as GIRK1a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.