NUR77 (NR4A1) (NM_002135) Human Untagged Clone

CAT#: SC118806

NR4A1 (untagged)-Human nuclear receptor subfamily 4, group A, member 1 (NR4A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NR4A1
• Synonyms: GFRP1; HMR; N10; NAK-1; NGFIB; NP10; NUR77; TR3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002135, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCTGTATCCAAGCCCAATATGGGACACCAGCACCGAGTCCGGGACCCCGTGACCACCTGGCAAGCG
  ACCCCCTGACCCCTGAGTTCATCAAGCCCACCATGGACCTGGCCAGCCCCGAGGCAGCCCCCGCTGCCCC
  CACTGCCCTGCCCAGCTTCAGCACCTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCAG
  CTGCCAGGAACAGTCCAGCCATGCTCCTCAGCCTCCTCCTCGGCCTCCTCCACATCCTCGTCCTCAGCCA
  CCTCCCCTGCCTCTGCCTCCTTCAAGTTCGAGGACTTCCAGGTGTACGGCTGCTACCCCGGCCCCCTGAG
  CGGCCCAGTGGATGAGGCCCTGTCCTCCAGTGGCTCTGACTACTATGGCAGCCCCTGCTCGGCCCCGTCG
  CCCTCCACGCCCAGCTTCCAGCCGCCCCAGCTCTCTCCCTGGGATGGCTCCTTCGGCCACTTCTCGCCCA
  GCCAGACTTACGAAGGCCTGCGGGCATGGACAGAGCAGCTGCCCAAAGCCTCTGGGCCCCCACAGCCTCC
  AGCCTTCTTTTCCTTCAGTCCTCCCACCGGCCCCAGCCCCAGCCTGGCCCAGAGCCCCCTGAAGTTGTTC
  CCCTCACAGGCCACCCACCAGCTGGGGGAGGGAGAGAGCTATTCCATGCCTACGGCCTTCCCAGGTTTGG
  CACCCACTTCTCCACACCTTGAGGGCTCGGGGATACTGGATACACCCGTGACCTCAACCAAGGCCCGGAG
  CGGGGCCCCAGGTGGAAGTGAAGGCCGCTGTGCTGTGTGTGGGGACAACGCTTCATGCCAGCATTATGGT
  GTCCGCACATGTGAGGGCTGCAAGGGCTTCTTCAAGCGCACAGTGCAGAAAAACGCCAAGTACATCTGCC
  TGGCTAACAAGGACTGCCCTGTGGACAAGAGGCGGCGAAACCGCTGCCAGTTCTGCCGCTTCCAGAAGTG
  CCTGGCGGTGGGCATGGTGAAGGAAGTTGTCCGAACAGACAGCCTGAAGGGGCGGCGGGGCCGGCTACCT
  TCAAAACCCAAGCAGCCCCCAGATGCCTCCCCTGCCAATCTCCTCACTTCCCTGGTCCGTGCACACCTGG
  ACTCAGGGCCCAGCACTGCCAAACTGGACTACTCCAAGTTCCAGGAGCTGGTGCTGCCCCACTTTGGGAA
  GGAAGATGCTGGGGATGTACAGCAGTTCTACGACCTGCTCTCCGGTTCTCTGGAGGTCATCCGCAAGTGG
  GCGGAGAAGATCCCTGGCTTTGCTGAGCTGTCACCGGCTGACCAGGACCTGTTGCTGGAGTCGGCCTTCC
  TGGAGCTCTTCATCCTCCGCCTGGCGTACAGGTCTAAGCCAGGCGAGGGCAAGCTCATCTTCTGCTCAGG
  CCTGGTGCTACACCGGCTGCAGTGTGCCCGTGGCTTCGGGGACTGGATTGACAGTATCCTGGCCTTCTCA
  AGGTCCCTGCACAGCTTGCTTGTCGATGTCCCTGCCTTCGCCTGCCTCTCTGCCCTTGTCCTCATCACCG
  ACCGGCATGGGCTGCAGGAGCCGCGGCGGGTGGAGGAGCTGCAGAACCGCATCGCCAGCTGCCTGAAGGA
  GCACGTGGCAGCTGTGGCGGGCGAGCCCCAGCCAGCCAGCTGCCTGTCACGTCTGTTGGGCAAACTGCCC
  GAGCTGCGGACCCTGTGCACCCAGGGCCTGCAGCGCATCTTCTACCTCAAGCTGGAGGACTTGGTGCCCC
  CTCCACCCATCATTGACAAGATCTTCATGGACACGCTGCCCTTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002135 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAAGACGAAACTTGGGGGAGTGC
  ACAGAAGAACTTCGGGAGCGCACGCGGGACCAGGGACCAGGCTGAGACTCGGGGCGCCAG
  TCCGGGCAGGGGCAGCGGGAGCCGGCCGGAGATGCCCTGTATCCAAGCCCAATATGGGAC
  ACCAGCACCGAGTCCGGGACCCCGTGACCACCTGGCAAGCGACCCCCTGACCCCTGAGTT
  CATCAAGCCCACCATGGACCTGGCCAGCCCCGAGGCAGCCCCCGCTGCCCCCACTGCCCT
  GCCCAGCTTCAGCACCTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCA
  GCTGCCAGGAACAGTCCAGCCATGCTCCTCAGCCTCCTCCTCGGCCTCCTCCACATCCTC
  GTCCTCAGCCACCTCCCCTGCCTCTGCCTCCTTCAAGTTCGAGGACTTCCAGGTGTACGG
  CTGCTACCCCGGCCCCCTGAGCGGCCCAGTGGATGAGGCCCTGTCCTCCAGTGGCTCTGA
  CTACTATGGCAGCCCCTGCTCGGCCCCGTCGCCCTCCACGCCCAGCTTCCAGCCGCCCCA
  GCTCTCTCCCTGAGATGGCTCCTTCGGCCACTTCTCGCCCAGCCAGACTTACGAAAGCCT
  GCGGGCATGGACAGAGCAGCTGCCCAAAGCCTCTGGGCCCNCACAGCCTNCAGCCTTCTT
  TTNCTTCAGTCCTACCACCGGCNCCCAGCCCCAGTCTGGCCCANAGCCCCCCTGAAAGGT
  GTCCCCTCACAGGGCACCCACCAGCTGGGGGAGGGGAGAGAGCTATTNCATGCCTACGGC
  NCTTNNCAGTTTGGCACCCACTCTCCACACCTTGAGGGCTCGGGGATCTGGATACACCCG
  TGACCTCACCAAGGCCCGGANCGNGCCCCAGNTGNAANTGAAGCCCCTGTGCTGGTGTGG
  GACAACCTTCATGCACATTATGTGNCGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002135 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACATGGCTA
  TTTTTTATTCAATATATTAAATATATTAATCCAAAAAAGTCACATACTATAAATCCAGGA
  AAATACACAAATATAAATGTCAAAATCTGTCATTTGTCTTCTTCCTAAAGTGACAGTTTA
  TGTACATGTGGAAGGAGGGTAGGAAAAAAGAGCCAGCAGGAGGCTGGAAAGGCTGCCCAA
  AAAGGCGGGGCCCATCCCTTCCTCCCAGCCCCGCTCCCACCTAAAGCTCTTTCTTCCTGT
  ATTTACAGCAGCACTGGGCTTATTTACAGCACGGGGCTATGTGTTAAAAACGGTTTATCT
  TACAAAAAACATAAAGGCCGGGGCCAGGCTGGGGGGGATAAAACAAATCGTGGGGTCACC
  CCCATGAAGGCATCCCCCTCCCCAAGGGCTTGAGCTCCCTGCAAACCTCCTGGAACAGGT
  GAAAAGGTGGAGTCATTCTGCAGCTCAAGCCCAGGCTTGGGGGTGCTCTGGGGGTCCGTG
  GACTAAAGGCACATGGGGTGGCATATGAGAGTGCGCATGTGCACACGTGTTCCCAGGCAG
  GGGTCAGAAGGGCACCGTGCCCATGAAGATCTTGTCAATGATGGGTGGAGGGGGCCCCAG
  TCCTCCAACTTTGAGTAAATAAGCGCTGCACGCCCTGGTGCACAGGTTCCCCACCTCGGC
  CATTTGCCCACAAATGTTACAGCATTTGTCTGTTGGGCCTTTCCACCAATTTGCCACGGG
  TTCTTTAGCCACTGGCAATGCGGTTTTCACCTCTTCCCCGCCGCGGTTCTTAACCCATCC
  GCCGGGTAAGGACAAGCCCATAGTTTGGAAGCAGGAACCCCAACCACTGGTAGGGACTTG
  AGAAGCCAATCCGTTATACCCCCCCCAACCCCGGCCTCTACCCGGTTTTCCACCCCCGAC
  CAAAAAAATTTTCTCCCTCGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002135
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002135.3, NP_002126.2
• RefSeq Size: 2699 bp
• RefSeq ORF: 1797 bp
• Locus ID: 3164
• Cytogenetics: 12q13.13
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (1) differs in the 5' structure, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 4. The encoded isoform (1) has a shorter N-terminus compared to isoform 3. Variants 1 and 2 encode the same isoform.