GNB1 (NM_002074) Human Untagged Clone

CAT#: SC118812

GNB1 (untagged)-Human guanine nucleotide binding protein (G protein), beta polypeptide 1 (GNB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GNB1
• Synonyms: MRD42
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002074 edited
  GAATTCGGCACGAGGCCGCGACGAGGGCGCTGAGACAAATTTACATGTATTGGAGACCAG
  ACCAGAAGCCCTTCTGAATTAAGATCTCACATTCTTGAAGGTGGCATTGAAGAGCACTAA
  GATCGGAAGATGAGTGAGCTTGACCAGTTACGGCAGGAGGCCGAGCAACTTAAGAACCAG
  ATTCGAGACGCCAGGAAAGCATGTGCAGATGCAACTCTCTCTCAGATCACAAACAACATC
  GACCCAGTGGGAAGAATCCAAATGCGCACGAGGAGGACACTGCGGGGGCACCTGGCCAAG
  ATCTACGCCATGCACTGGGGCACAGACTCCAGGCTTCTCGTCAGTGCCTCGCAGGATGGT
  AAACTTATCATCTGGGACAGCTACACCACCAACAAGGTCCACGCCATCCCTCTGCGCTCC
  TCCTGGGTCATGACCTGTGCATATGCCCCTTCTGGGAACTATGTGGCCTGCGGTGGCCTG
  GATAACATTTGCTCCATTTACAATCTGAAAACTCGTGAGGGGAACGTGCGCGTGAGTCGT
  GAGCTGGCAGGACACACAGGTTACCTGTCCTGCTGCCGATTCCTGGATGACAATCAGATC
  GTCACCAGCTCTGGAGACACCACGTGTGCCCTGTGGGACATCGAGACCGGCCAGCAGACG
  ACCACGTTTACCGGACACACTGGAGATGTCATGAGCCTTTCTCTTGCTCCTGACACCAGA
  CTGTTCGTCTCTGGTGCTTGTGATGCTTCAGCCAAACTCTGGGATGTGCGAGAAGGCATG
  TGCCGGCAGACCTTCACTGGCCACGAGTCTGACATCAATGCCATTTGCTTCTTTCCAAAT
  GGCAATGCATTTGCCACTGGCTCAGACGACGCCACCTGCAGGCTGTTTGACCTTCGTGCT
  GACCAGGAGCTCATGACTTACTCCCATGACAACATCATCTGCGGGATCACCTCTGTCTCC
  TTCTCCAAGAGCGGGCGCCTCCTCCTTGCTGGGTACGACGACTTCAACTGCAACGTCTGG
  GATGCACTCAAAGCCGACCGGGCAGGTGTCTTGGCTGGGCATGACAACCGCGTCAGCTGC
  CTGGGCGTGACTGACGATGGCATGGCTGTGGCGACAGGGTCCTGGGATAGCTTCCTCAAG
  ATCTGGAACTAACGCCAGTAGCATGTGGATGCCATGGAGACTGGAAGACCATTCCAACTT
  GGACGCGTTACCATGAGAGCATATCCTATCCAACCGTACTAACGTGGACACCCTACACCT
  CCCCTCAGAACTTCAAAAGGGCAAGATCTTTTTTCCTTCACTTATTGCTGAAACCAAGAG
  CACAATTCCCATTGAGAGAAAGATCTCTGTGCTGTAAACTAAAACAAATTGTGCATTCCT
  TCCGGGGCCATCGTCTTTGTTTTCTTTTTTGTCTTGAATGAATTTTAAAAGGAAATATAT
  AATAAAAATGTTAACCAGAAGGTAAACTTGAGTGTAATTGTCAGACAGACACACTTTTCC
  ACCAGTGTATTTGAATTTTAGACCAGTGACCCTGTTTTGTGGCATTCATGCAAAACATGC
  TGAGGGCTTTGTTCATCTGGTCATCGTGTCCAAATTTCAGTCATGTTTGTAGCAAGATTT
  TGGAAGCATTCATATTTCCTTTTTAAAATGTATTCCTTTGTGTTCAACAGTTAATCAAAA
  CCAGAGAGTCTAGGGCAGCCTCTCTGATGTTGTCAATGATGTAAATTCAGTCCCTGGTTT
  TTAATTTTCTGTCTGATGTCACAGATCATTGTTGCACACAAACGTGGCATAGAAAAGAAC
  ATGTTCAGAAGCCATGGGGCCAAGCACATGCGGGGACGGTCTCAAATGCGTGATCAGAGA
  ATCCTTCACCTTTGCTGAAAAGTGAGCTCAGATCCAGCACCATGTTCCTCCTGACCCATC
  CTGTCTATCTTCTCAGTTGAGTTTTTAATCTCACTTTGGGTTTCCTTGTGAAGTTGGAGG
  GAAGTTTATAATAGCCTAACACTACCCCACCCCCAACTAGGAGGAACCTCTGTTTTCAAG
  AGAGATGCCTGTCCTGTGCTTGGATAGTCAGTCAATTATTTGTGTATGAAACAATGTACA
  AATCAATGTTTTGAAAATAATGATCTCAGACTTTCTAAGTTAAATTTTAAAAATTTTGAT
  TGTTTGCCATATTGGGTGGGTTTACTCTTAGAATCGCATGCTGTAGAAATGCTCAAAAGT
  GCATATGGGACTCAGTCCTTAGGTGTTCTTTTTCTTTTAAGAAATAACCTCTTACAGTTG
  TAACCATTGCGGCTCTGTCCACTTCTCGTTGCTGCTCTGTGGCACATATCGGAAGCAGTA
  CAGCGCGCGGCTCTACACGCTTGGGTAGCGGGATAAGTCACTGTTTTCTTTATTTCTTTA
  AAAAAAAAAAAGTTCTGTTGCAAACGACTGCTGTTGGATTCTGAGGGTGGGGAGGGAGAG
  AGAGGGAGGGAGAGGGAGTGAAGAGCCTGCCCTCCTATATGGATTCTTCAGGGCCCTCCA
  CATCTGAGGTGGCTCATTCCCATCACACACAGATTGTCCTGGTGTTCATTTCAAGGCCAG
  TGTTCAGCAGCAGCGTTTGGAAAGCAGGTTCTGTGGGACCCCCCGCCCCGCCCCCCGCAC
  TCCTTCATAGCAGCAGTAGTGGCTTCTCCATCCTGTTTTCTGCAACATTCTATACAAAAC
  TGTGCTGTGACCTTGCGGTAGGCCTGGATCTGGCAAAGAGAATACAAATGAAACCCCTTC
  TTTCTCTTTCCGTCCAACAACTCTGTAGAGCTCTCTGCACCCTTACCCCTTTCCACCTTT
  TGTATTTAATTTTAAAGTCAGTGTACTGCAAGGAAGCTGGATGCAAGATAGATACTATAT
  TAAACTGTACTGTTATTTAAGATGTAATAAAGCAGTTTGACATGAAAAAAAAAAAAAAAA
  AACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002074 unedited
  ATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCCGCGACGAGGGCGCTGAGACA
  AATTTACATGTATTGGAGACCAGACCAGAAGCCCTTCTGAATTAAGATCTCACATTCTTG
  AAGGTGGCATTGAAGAGCACTAAGATCGGAAGATGAGTGAGCTTGACCAGTTACGGCAGG
  AGGCCGAGCAACTTAAGAACCAGATTCGAGACGCCAGGAAAGCATGTGCAGATGCAACTC
  TCTCTCAGATCACAAACAACATCGACCCAGTGGGAAGAATCCAAATGCGCACGAGGAGGA
  CACTGCGGGGGCACCTGGCCAAGATCTACGCCATGCACTGGGGCACAGACTCCAGGCTTC
  TCGTCAGTGCCTCGCAGGATGGTAAACTTATCATCTGGGACAGCTACACCACCAACAAGG
  TCCACGCCATCCCTCTGCGCTCCTCCTGGGTCATGACCTGTGCATATGCCCCTTCTGGGA
  ACTATGTGGCCTGCGGTGGCCTGNATAACATTTGCTCCATTTACAATCTGAAAACTCGTG
  AGGGGAACGTGCGCGTGAGTCGTGAGCTGGCAGGACACACAGGTTACCTGTCCTGCTGCC
  GATTCCTGGNATGACATCAGATCGTCACCAGCTCTGGAGACACCACGTGTGCCCTGTGGG
  ACATCGAGACCGGCCAGCAGACGACCACGTTTACCGGACACACTGGAGATGTCATGAGCC
  TTTCTCTTGCTCCTGACACCAGACTGTTCGTCTCTGGTGCTTGTGATGCTTCACCCAAAC
  TCTGGGATGTGCGAAAAGGCATGTGCCCGNCAAACCCTTACTGGCCACGAGTCTGACATC
  AATGCCATTTGCTCCTTTTCAATGGGCATGCATTTGCCACTGGCTAAAACAACGCACCTG
  CAGCTGTTTGACCTTGTGCTGACCAGAACCATGACTACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002074 unedited
  GGGTTTGACTCTGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTCA
  TGTCAAACTGCTTTATTACATCTTAAATAACAGTACAGTTTAATATAGTATCTATCTTGC
  ATCCAGCTTCCTTGCAGTACACTGACTTTAAAATTAAATACAAAAGGTGGAAAGGGGTAA
  GGGTGCAGAGAGCTCTACAGAGTTGTTGGACGGAAAGAGAAAGAAGGGGTTTCATTTGTA
  TTCTCTTTGCCAGATCCAGGCCTACCGCAAGGTCACAGCACAGTTTTGTATAGAATGTTG
  CAGAAAACAGGATGGAGAAGCCACTACTGCTGCTATGAAGGAGTGCGGGGGGCGGGGCGG
  GGGGTCCCACAGAACCTGCTTTCCAAACGCTGCTGCTGAACACTGGCCTTGAAATGAACA
  CCAGGACAATCTGTGTGTGATGGGAATGAGCCACCTCAGATGTGGAGGGCCCTGAAAATC
  CATATAGGAGGGCAGGCTCTTCACTCCCTTCTCCTCCCTCTCTCTCCCTCCCCACCCTCA
  AATTCAACAGGAGTCGTTTGCAACAGAACTTTTTTTTTTTTAAAGGAATCAAAAAAACCA
  GGACTTATTCCGGTACCCAAGCGTGTTAAGCCCCGCCCTGTACTTGTTTCCATTTGTCCC
  CAAAACAGTAACGAGAAGGGACCGAACCCCAATGGTTCCACTGTAAGAGTTTTTTTTTAA
  AGGAAAAAAACCCTAGGACTGAGCCCCATTGCCCTTTGACCTTTCTCAGCAGGCCATTTT
  AAAAAAAACCCCCCATTGTGCAAACATCAAATTTTAAATTACCTTAAAACTTCGAACTTT
  TTCTCAAAATTGATTGGCATTGGTAAACCCAAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002074
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002074.2, NP_002065.1
• RefSeq Size: 3147 bp
• RefSeq ORF: 1023 bp
• Locus ID: 2782
• Cytogenetics: 1p36.33
• Domains: WD40
• Protein Pathways: Chemokine signaling pathway, Taste transduction
• Gene Summary: 'Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform.