Granzyme K (GZMK) (NM_002104) Human Untagged Clone

CAT#: SC118834

GZMK (untagged)-Human granzyme K (granzyme 3, tryptase II) (GZMK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GZMK
• Synonyms: TRYP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118834 sequence for NM_002104 edited (data generated by NextGen Sequencing)
  ATGACTAAGTTTTCTTCCTTTTCTCTGTTTTTCCTAATAGTTGGGGCTTATATGACTCAT
  GTGTGTTTCAATATGGAAATTATTGGAGGGAAAGAAGTGTCACCTCATTCCAGGCCATTT
  ATGGCCTCCATCCAGTATGGCGGACATCACGTTTGTGGAGGTGTTCTGATTGATCCACAG
  TGGGTGCTGACAGCAGCCCACTGCCAATATCGGTTTACCAAAGGCCAGTCTCCCACTGTG
  GTTTTAGGCGCACACTCTCTCTCAAAGAATGAGGCCTCCAAACAAACACTGGAGATCAAA
  AAATTTATACCATTCTCAAGAGTTACATCAGATCCTCAATCAAATGATATCATGCTGGTT
  AAGCTTCAAACAGCCGCAAAACTCAATAAACATGTCAAGATGCTCCACATAAGATCCAAA
  ACCTCTCTTAGATCTGGAACCAAATGCAAGGTTACTGGCTGGGGAGCCACCGATCCAGAT
  TCATTAAGACCTTCTGACACCCTGCGAGAAGTCACTGTTACTGTCCTAAGTCGAAAACTT
  TGCAACAGCCAAAGTTACTACAACGGCGACCCTTTTATCACCAAAGACATGGTCTGTGCA
  GGAGATGCCAAAGGCCAGAAGGATTCCTGTAAGGGTGACTCAGGGGGCCCCTTGATCTGT
  AAAGGTGTCTTCCACGCTATAGTCTCTGGAGGTCATGAATGTGGTGTTGCCACAAAGCCT
  GGAATCTACACCCTGTTAACCAAGAAATACCAGACTTGGATCAAAAGCAACCTTGTCCCG
  CCTCATACAAATTAA
  
  Clone variation with respect to NM_002104.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002104 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGGATCAACACATTTCA
  TCTGGGCTTCTTAAATCTAAATCTTTAAAATGACTAAGTTTTCTTCCTTTTCTCTGTTTT
  TCCTAATAGTTGGGGCTTATATGACTCATGTGTGTTTCAATATGGAAATTATTGGAGGGA
  AAGAAGTGTCACCTCATTCCAGGCCATTTATGGCCTCCATCCAGTATGGCGGACATCACG
  TTTGTGGAGGTGTTCTGATTGATCCACAGTGGGTGCTGACAGCAGCCCACTGCCAATATC
  GGTTTACCAAAGGCCAGTCTCCCACTGTGGTTTTAGGCGCACACTCTCTCTCAAAGAATG
  AGGCCTCCAAACAAACACTGGAGATCAAAAAATTTATACCATTCTCAAGAGTTACATCAG
  ATCCTCAATCAAATGATATCATGCTGGTTAAGCTTCAAACAGCCGCAAAACTCAATAAAC
  ATGTCAAGATGCTCCACATAAGATCCAAAACCTCTCTTAGATCTGGAACCAAATGCAAGG
  TTACTGGCTGGGGAGCCACCGATCCAGATTCATTAAGACCTTCTGACACCCTGCGAGAAG
  TCACTGTTACTGTCCTAAGTCGAAAACTTTGCAACAGCCAAAGTTACTACAACGGGCGAC
  CCTTTTATCACCAAAGACATGGNTCTGTGCAGGAGATGCCAAAGGCCAGAGGGATTCCTG
  TANGGGTGACTCAGGGGGCCCCTTGATCTGTAAAGGTGTCTTCCCGCTATAGTCTCTGGA
  GTCATGAATGTGGTGTTGCCACAAGCCTGGAATCTACACCCTGTTACCAAGAATACCAGA
  CTTGGATCAAAGCACCTTTGTCCGNCTCTACAAATAAGTACAATAATTTATTGGAGCACT
  TGCTCTTTTTCTATAGCTCCAGGTAAAGTGGGTTAGTAAGCAACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002104 unedited
  CCGCAATCTAGTGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTGGAAAGGCCTCTAAATTTTTTTTAAAATAAAACCCCCCTGG
  GGGAAAATACCCCCGGGGTTCCAGGGAAAAAAAACCTGGTTCCCTTATAAAAAGGCTTAC
  AAGGGCCAAAATGGGCCCCCTTTGGGCCCTGGTTTTCTTACCCCCAACTTTTACCTGGGG
  GCCTTTTTGGAAAAAAAAAACCAGGGCCTCCCAAAAAATTTTTTGGAACCTAATTTGGAT
  GAAGGGGGACAAGGGTGCTTTTGATCCAAAGCTGGGTTTTCTTGGGTAACAGGGGGGAAA
  TTCCAAGCTTTGGGGGAACCCCCCATTTATGACCCCCAAAAACTTTAGCGGGGAAAACAC
  CTTTACAGATAAAGGGGCCCCCTGGGTCCCCCTTACAGGAATCCCTTTGGGCCTTGGGTT
  TTTCTGGACAAAACCTGGTTTTGTGGGAAAAAGGGGCCCCCGTGGATTAACCTTGGCTGG
  TGCCAAATTTTTACTCTTTGGCAGTAACCCTGGCTTCTCTCGGGGGGCAAAAGGTTTTTA
  AGAATTTGGATCGGGGGTCCCCCCCCAGTACCCTTGCTTTGGTCCCAAATTAAAAAAGGT
  CCGCCCTCTCGGGGGCCCCCGGCTGCTCTTTGGCTCTCCGGCGCGTCATCTCTCCCCCCC
  CCTCTTCTCTTGGGGCCCCGTCCTCTCTGGGCACGCGCTATTCTCTGCCCCACGTTGGTT
  GGGGGGCCCTTCTCTGCGAAAAGGCCGCCCTACCCCTCGGGGATCGGCCCCGGCCTTCCT
  TTCGCGGGCCCCCTGTCCCGCCCCCCGGCTGTTTTCTCCCTCTCTATTCCCTCTCCCCCC
  GCTTCTGCCCCCGGGCCCGGGTGGGTCTTGTCTCTTTCCTTTGCTTCCTACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002104
• Insert Size: 1220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002104.2, NP_002095.1
• RefSeq Size: 1074 bp
• RefSeq ORF: 795 bp
• Locus ID: 3003
• Cytogenetics: 5q11.2
• Domains: Tryp_SPc
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Gene Summary: 'This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]'