GAPDH (NM_002046) Human Untagged Clone

CAT#: SC118869

GAPDH (untagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GAPDH
• Synonyms: G3PD; GAPD; HEL-S-162eP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002046 edited
  ATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTCACCAGG
  GCTGCTTTTAACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTGACCTC
  AACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCACCGTC
  AAGGCTGAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGGAGCGA
  GATCCCTCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCACTGGC
  GTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGGTCATC
  ATCTCTGCCCCCTCTGCTGACGCCCCCATGTTCGTCATGGGTGTGAACCATGAGAAGTAT
  GACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAGCACCCCTG
  GCCAAGGTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCC
  ATCACTGCCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGC
  GGGGCTCTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAAGGCTGTGGGCAAGGTC
  ATCCCTGAGCTGAACGGGAAGCTCACTGGCATGGCCTTCCGTGTCCCCACTGCCAACGTG
  TCAGTGGTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGAAG
  GTGGTGAAGCAGGCGTCGGAGGGCCCCCTCAAGGGCATCCTGGGCTACACTGAGCACCAG
  GTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTCCACCTTCGACGCTGGGGCTGGC
  ATTGCCCTCAACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAACGAATTTGGCTAC
  AGCAACAGGGTGGTGGACCTCATGGCCCACATGGCCTCCAAGGAGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002046 unedited
  ACGAGGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTGAN
  ACACCATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTCA
  CCAGGGCTGCTTTTAACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTG
  ACCTCAACTACATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCA
  CCGTCAAGGCTGAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGG
  AGCGAGATCCCTCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCA
  CTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGG
  TCATCATCTCTGCCCCCTCTGCTGACGCCCCCATGTTCGTCATGGGTGTGAACCATGAGA
  AGTATGACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAACAC
  CCCTGGCCAAGGGTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTC
  CATGCCATCACTGCCACCCAAAANACTGTGGGATGGCCCCCTNCGGGAAACTGTGGGCGT
  GATGGCCCGNGGGGCTCTNCANAACATTATNCCTGCCTNTACTGGGCGCTGCCAAGGCTG
  GGGGGCAAGGTCATTCCTTGAACTGAACGGGAANCTCACTGGCATGGGCCTTTCGTGTCC
  CCCTGGCAACGTGTTAGGGGGGGGACCTGACCTGCCGTTAAAAAAANCTGCCAAATTGAT
  GACCTCAAAAAGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002046 unedited
  TTGGTATGGCAACTTCCAGGNCCAGGTANAGCACTGGGGNAGGGTCACAGGNATGCCACC
  CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTT
  TTTTTTTTTGGTTGAGCACAGGGTACTTTATTGATGGTACATGACAAGGTGCGGCTCCCT
  AGGCCCCTCCCCTCTTCAAGGGGTCTACATGGAAACTGTGAGGAGGGGAGATTCAGTGTG
  GTGGGGGACTGAGTGTGGCAGGGACTCCCCAGCAGTGAGGGTCTCTCTCTTCCTCTTGTG
  CTCTTGCTGGGGCTGGTGGTCCAGGGGTCTTACTCCTTGGAGGCCATGTGGGCCATGAGG
  TCCACCACCCTGTTGCTGTAGCCAAATTCGTTGTCATACCAGGAAATGAGCTTGACAAAG
  TGGTCGTTGAGGGCAATGCCAGCCCCAGCGTCNAAGGTGGAGGAGTGGGTGTCGCTGTTG
  AAGTCAGAGGAGACCACCTGGTGCTCAGTGTANCCCAGGATGCCCTTGAGGGGGCCCTCC
  GACGCCTGCTTCACCACCTTCTTGATGTCATCATATTTGGCAGGTTTTTCTAGACGGCAG
  GTCAGGTCCACCACTGACACGTTGGCAGTGGGGACACGGAAGGCCATGCCAGTGAGCTTC
  CCGTTCAGCTCAGGGATGACCTTGCCCACAGCCTTGGCAGCGCCAGTAAAGGCAGGGATG
  ATGTTCTGGAGACCCCGCNGNCATCACGCACAGTTTCCCGGAGGGGGCATCCACAGTCTT
  CTGGGTGCANNGATGGCATGGACCTGTGTCATGATTCCTTCACGATACCCAAAGTGCATG
  GATGACCTGGNCAGGGTGCTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002046
• Insert Size: 1290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002046.2, NP_002037.2
• RefSeq Size: 1283 bp
• RefSeq ORF: 1008 bp
• Locus ID: 2597
• Cytogenetics: 12p13.31
• Domains: gpdh
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).