FHIT (NM_002012) Human Untagged Clone

CAT#: SC118885

FHIT (untagged)-Human fragile histidine triad gene (FHIT), transcript variant 1


  "NM_002012" in other vectors (5)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol FHIT
Synonyms AP3Aase; FRA3B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118885 sequence for NM_002012 edited (data generated by NextGen Sequencing)
ATGTCTTTCAGATTTGGCCAACATCTCATCAAGCCCTCTGTAGTGTTTCTCAAAACAGAA
CTGTCCTTCGCTCTTGTGAATAGGAAACCTGTGGTACCAGGACATGTCCTTGTGTGCCCG
CTGCGGCCAGTGGAGCGCTTCCATGACCTGCGTCCTGATGAAGTGGCCGATTTGTTTCAG
ACGACCCAGAGAGTCGGGACAGTGGTGGAAAAACATTTCCATGGGACCTCTCTCACCTTT
TCCATGCAGGATGGCCCCGAAGCCGGACAGACTGTGAAGCACGTTCACGTCCACGTTCTT
CCCAGGAAGGCTGGAGACTTTCACAGGAATGACAGCATCTATGAGGAGCTCCAGAAACAT
GACAAGGAGGACTTTCCTGCCTCTTGGAGATCAGAGGAGGAAATGGCAGCAGAAGCCGCA
GCTCTGCGGGTCTACTTTCAGTGA

Clone variation with respect to NM_002012.2
6 g=>t;294 t=>c
>OriGene 5' read for NM_002012 unedited
NGGGTTCAAAATTTGNTATACGACTCACTATAGGGCGGCCGCGNAATCGGCACGAGGCTC
TGTCCGGTCACAGGACTTTTTGCCCTCTGTTCCCGGGTCCCTCAGGCGGCCACCCAGTGG
GCACACTCCCAGGCGGCGCTCCGGCCCCGCGCTCCCTCCCTCTGCCTTTCATTCCCAGCT
GTCAACATCCTGGAAGCTTTGAAGCTCAGGAAAGAAGAGAAATCCACTGAGAACAGTCTG
TAAAGGTCCGTAGTGCTATCTACATCCAGACGGTGGAAGGGAGAGAAAGAGAAAGAAGGT
ATCCTAGGAATACCTGCCTGCTTAGACCCTCTATAAAAGCTCTGTGCATCCTGCCACTGA
GGACTCCGAAGAGGTAGCAGTCTTCTGAAAGACTTCAACTGTGAGGACATGTCTTTCAGA
TTTGGCCAACATCTCATCAAGCCCTCTGTAGTGTTTCTCAAAACAGAACTGTCCTTCGCT
CTTGTGAATAGGAAACCTGTGGTACCAGGACATGTCCTTGTGTGCCCGCTGCGGCCAGTG
GAGCGCTTCCATGACCTGCGTCCTGATGAAGTGGCCGATTTGTTTCAGACGACCCAGAGA
GTCGGGACAGTGGTGGAAAAACATTTCCATGGGACCTCTCTCACCTTTTCCATGCAGGAT
GGCCCCGAAGCCGGACAGACTGTGAAGCACGTTCACGTCCACGTTCTTCCCAGGAAGGCT
GGAGACTTTCACAGGAATGACAGCATCTATGAGGAGCTCCAGAAACATGACAGGNAGGAC
TTTCCTGCCTCTTGGAGATCAGAGGAGGAAATGGCAGCAGAAGCCGCAGCTCTGCGGGTC
TACTTTCAGTGACACAGGTAAAGACTGCATTTCGTCATTACCCAGAACCCTCCCGTGGCC
TCAGGAAAGAGG
>OriGene 3' read for NM_002012 unedited
TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TGCAGCAGAAAAGGAAGTTTAATCATAAGGCTGCCGAATAAGGAGACAGGGGGAAACCTC
AAATCTGCCTGTCTGAGCCGTTTAGGTCTAGGTATTTTAAGGGAGTTGGAGTGACCGAGG
TGGGGGATCACTGGTTGAAAAATACAGGATGGTGAGATGAAAAAACTGCATTTTCATGCT
GATTCAGTTCCTCTTGGGGAAAGGCGGGGGGGCGGTCTTCAAACTGGTTGGCAATAGCTC
TTTTGCTGGAATTCAGGATCTGAAAAACATCTTAAGCCATTTTTAAACAAACAAAGGCCT
TATGATTCTAACATCAAAAATCTTCTCTGTAGGAACAATGAGGAGGCAAATGGTTAGCAT
CTGGTGCTCTGGGACTTTTGGTTACCAAGAAGTGGGCCAAAGTGCAGCCTGATTAATGCT
TAATTACAACTATATTTCTGTCCAGAACCCAGCATCTAATTCTTGTTAACCCTGTGAGGA
TGGTTTCAATAGAATGTTTCCATTGTCCTTAAAGGGAGGTATAGTTAGTCCCATTATACT
CCAGGTAATGATGACAGTCTTGGCAAAATCTTAGGACCCTAAGTTGTTTGCCACCTCACA
ACCTATATTATCCGTCCTAGTCTCAGTTACCANCATCTGACTGCTGGGAAGACTGAGGGT
GATTTGTTTGGTCTGTGCACAAAACCTTTAAAGAGAATACACATGTTAGTTAACTTAAAA
ATCCACAGGACTGTGGGCATAGTTTGTGTAACAAGTTAGGGAAATATCTTTCCTGGTAGA
TGTACAGGGAAGTCATTTACCTGGAAAACAAGAGGGGTACGCCTTCAGCA
Restriction Sites NotI-NotI     
ACCN NM_002012
Insert Size 2700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002012.1, NP_002003.1
RefSeq Size 1095 bp
RefSeq ORF 444 bp
Locus ID 2272
Cytogenetics 3p14.2
Domains HIT
Protein Pathways Non-small cell lung cancer, Purine metabolism, Small cell lung cancer
Gene Summary 'The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1-4 all encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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