FKBP52 (FKBP4) (NM_002014) Human Untagged Clone

CAT#: SC118889

FKBP4 (untagged)-Human FK506 binding protein 4, 59kDa (FKBP4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FKBP4
• Synonyms: FKBP51; FKBP52; FKBP59; HBI; Hsp56; p52; PPIase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118889 sequence for NM_002014 edited (data generated by NextGen Sequencing)
  ATGACAGCCGAGGAGATGAAGGCGACCGAGAGCGGGGCGCAGTCGGCGCCGCTGCCCATG
  GAGGGAGTGGACATCAGCCCCAAACAGGACGAAGGCGTGCTGAAGGTCATCAAGAGAGAG
  GGCACAGGTACAGAGATGCCCATGATTGGGGACCGAGTCTTTGTCCACTACACTGGCTGG
  CTATTAGATGGCACAAAGTTTGACTCCAGTCTGGATCGCAAGGACAAATTCTCCTTTGAC
  CTGGGAAAAGGGGAGGTCATCAAGGCTTGGGACATTGCCATAGCCACCATGAAGGTGGGG
  GAGGTGTGCCACATCACCTGCAAACCAGAATATGCCTACGGTTCAGCAGGCAGTCCTCCA
  AAGATTCCCCCCAATGCCACGCTTGTATTTGAGGTGGAGTTGTTTGAGTTTAAGGGAGAA
  GATCTGACGGAAGAGGAAGATGGCGGAATCATTCGCAGAATACAGACTCGCGGTGAAGGC
  TATGCTAAGCCCAATGAGGGTGCTATCGTGGAGGTTGCACTGGAAGGGTACTACAAGGAC
  AAGCTCTTTGACCAGCGGGAGCTCCGCTTTGAGATTGGCGAGGGGGAGAACCTGGATCTG
  CCTTATGGTCTGGAGAGGGCCATTCAGCGCATGGAGAAAGGAGAACATTCCATCGTGTAC
  CTCAAGCCCAGCTATGCTTTTGGCAGTGTTGGGAAGGAAAAGTTCCAAATCCCACCAAAT
  GCTGAGCTGAAATATGAATTACACCTCAAGAGTTTTGAAAAGGCCAAGGAGTCTTGGGAG
  ATGAATTCAGAAGAGAAGCTGGAACAGAGCACCATAGTGAAAGAGCGGGGCACTGTGTAC
  TTCAAGGAAGGTAAATACAAGCAAGCTTTACTACAGTATAAGAAGATCGTGTCTTGGCTG
  GAATATGAGTCTAGTTTTTCCAATGAGGAAGCACAGAAAGCACAGGCCCTTCGACTGGCC
  TCTCACCTCAACCTGGCCATGTGTCATCTGAAACTACAGGCCTTCTCTGCTGCCATTGAA
  AGCTGTAACAAGGCCCTAGAACTGGACAGCAACAACGAGAAGGGCCTCTTCCGCCGGGGA
  GAGGCCCACCTGGCCGTGAATGACTTTGAACTGGCACGGGCTGATTTCCAGAAGGTCCTG
  CAGCTCTACCCCAACAACAAAGCCGCCAAGACCCAGCTGGCTGTGTGCCAGCAGCGGATC
  CGAAGGCAGCTTGCCCGGGAGAAGAAGCTCTATGCCAATATGTTTGAGAGGCTGGCTGAG
  GAGGAGAACAAGGCCAAGGCAGAGGCTTCCTCAGGAGACCATCCCACTGACACAGAGATG
  AAGGAGGAGCAGAAGAGCAACACGGCAGGGAGCCAGTCTCAGGTGGAGACAGAAGCATAG
  
  Clone variation with respect to NM_002014.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002014 unedited
  GCTAGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCCGG
  ATAAACGGCGCGCCGCGCGGAGATGACAGCCGAGGAGATGAAGGCGACCGAGAGCGGGGC
  GCAGTCGGCGCCGCTGCCCATGGAGGGAGTGGACATCAGCCCCAAACAGGACGAAGGCGT
  GCTGAAGGTCATCAAGAGAGAGGGCACAGGTACAGAGATGCCCATGATTGGGGACCGAGT
  CTTTGTCCACTACACTGGCTGGCTATTAGATGGCACAAAGTTTGACTCCAGTCTGGATCG
  CAAGGACAAATTCTCCTTTGACCTGGGAAAAGGGGAGGTCATCAAGGCTTGGGACATTGC
  CATAGCCACCATGAAGGTGGGGGAGGTGTGCCACATCACCTGCAAACCAGAATATGCCTA
  CGGTTCAGCAGGCAGTCCTCCAAAGATTCCCCCCAATGCCACGCTTGTATTTGAGGTGGA
  GTTGTTTGAGTTTAAGGGAGAAGATCTGACGGAAGAGGAAGATGGCGGAATCATTCGCAG
  AATACAGACTCGCGGTGAAGGCTATGCTAAGCCCAATGAGGGTGCTATCGTGGAGGTTGC
  ACTGGAAGGGTACTACAAGGACAAGCTCTNTGACCAGCGGGAGCTCCGCTTTGAGATTGG
  CGAGGGGGAGAACCTGGATCTGCCTTATGGTCTGGAGAGGGCCATTCAGCGCATGGAGAA
  AGGGAGACATTCCATCGTGTACCTCAAGCCAGCTATGCTTTTGGCAGTNGTGGGAAGGAA
  AAGTTTCCAATCCCACCAATGCTGAGCTGAATATGAATTACACCTCAGAAGTTTGAAAAG
  CCCAGGAGTCTTGGGAGAATGATTCAGAAAGAAAGCTGGAACAGNACACCATAGTGAAAG
  ACGGGGCACTGTGTACTCCAGGGAAGGTAATACCAGCAAGCTTTACTANCAGTATAGAAA
  GATCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002014 unedited
  GACCGCGGCCGTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTTCCACTTTATTGCT
  CAAGCATGGAACTTGAGGCAAGCCTGGACCTTCAGCAACGTGCAGAGAAGAGGCTGAGGG
  GATTTTGGGACACAACCTGGCCGAGAGGGGAAACTGACAGGGGAAATACCAGAACAGGGA
  ACAGGGGGTGGTCACCATGACACCAGAAGACATCATCAGCCACACCGTCGGGGGTGAGGA
  AGGGAACAGAGAGAAGAAATGGCATAGCAGTGGTTTTTCCCATGTCCCTAGCACCCACAT
  GGAGGCCACATTTGGAAATGGACTGGGAGAGGGAAAGGATGAGGAAAACGGCTGCCCAGG
  AGTCTCCCTCTCCACCCTGGGTTCTTATCAGACCTCATCCCTACCACTTGCCTTTTCTAC
  CCTTAGAAAATGGACCTAACAGAGGGAGCAAAATAAATTAACATTCTGATGAATATATAT
  GGATGGACATATGTTCATGTGCAATGGAAGGAAGGGAAAAGGGGCTGACACCATCTAAAA
  TGATTCCCCAACCTAATTCCCCCACCCTAAACCTTTTCCCCCTTCCCCTTAAGCCCCCCT
  TCCCACCCGTATGGAAATAAAAGGGGTTCATTCCTCCAAATTTTTCAATTTTTTCAAAAA
  AACCAGGGCTGAACGGGGAATCTGGGGGCCAGCACCCCCCAAAATAGGCCTTTGGAAAGA
  GGTTTTCTTTTTTTCCCCCCCCTAAAAAGGTCCCCGCCCCGGAGCGCTCTTTCGGTCCCT
  CTTTTCCTTTACGGCCCCATGGTGTTTTCTTCTAGAAACTATCTTCTCCCCTCTTTTCTT
  CCCTCCACCCCCCCCCTCAAAAAATTGTGAAAAATCTTCTTCCTCCCACCCCATCTCTTC
  TTTCATTACGGCTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002014
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002014.2, NP_002005.1
• RefSeq Size: 2251 bp
• RefSeq ORF: 1380 bp
• Locus ID: 2288
• Cytogenetics: 12p13.33
• Domains: FKBP, TPR
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]'