Endothelin 1 (EDN1) (NM_001955) Human Untagged Clone

CAT#: SC118900

EDN1 (untagged)-Human endothelin 1 (EDN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDN1
• Synonyms: ARCND3; ET1; HDLCQ7; PPET1; QME
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118900 sequence for NM_001955 edited (data generated by NextGen Sequencing)
  ATGGATTATTTGCTCATGATTTTCTCTCTGCTGTTTGTGGCTTGCCAAGGAGCTCCAGAA
  ACAGCAGTCTTAGGCGCTGAGCTCAGCGCGGTGGGTGAGAACGGCGGGGAGAAACCCACT
  CCCAGTCCACCCTGGCGGCTCCGCCGGTCCAAGCGCTGCTCCTGCTCGTCCCTGATGGAT
  AAAGAGTGTGTCTACTTCTGCCACCTGGACATCATTTGGGTCAACACTCCCGAGCACGTT
  GTTCCGTATGGACTTGGAAGCCCTAGGTCCAAGAGAGCCTTGGAGAATTTACTTCCCACA
  AAGGCAACAGACCGTGAGAATAGATGCCAATGTGCTAGCCAAAAAGACAAGAAGTGCTGG
  AATTTTTGCCAAGCAGGAAAAGAACTCAGGGCTGAAGACATTATGGAGAAAGACTGGAAT
  AATCATAAGAAAGGAAAAGACTGTTCCAAGCTTGGGAAAAAGTGTATTTATCAGCAGTTA
  GTGAGAGGAAGAAAAATCAGAAGAAGTTCAGAGGAACACCTAAGACAAACCAGGTCGGAG
  ACCATGAGAAACAGCGTCAAATCATCTTTTCATGATCCCAAGCTGAAAGGCAAGCCCTCC
  AGAGAGCGTTATGTGACCCACAACCGAGCACATTGGTGA
  
  Clone variation with respect to NM_001955.4
  318 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001955 unedited
  CTCCGTAACCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
  AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC
  CGCGAATTCGGCACGAGGCATCTGCGCCAGGCGAACGGGTCCTGCGCCTCCTGCAGTCCC
  AGCTCTCCACCGCCGCGTGCGCCTGCAGACGCTCCGCTCGCTGCCTTCTCTCCTGGCAGG
  CGCTGCCTTTTCTCCCCGTTAAAGGGCACTTGGGCTGAAGGATCGCTTTGAGATCTGAGG
  AACCCGCAGCGCTTTGAGGGACCTGAAGCTGTTTTTCTTCGTTTTCCTTTGGGTTCAGTT
  TGAACGGGAGGTTTTTGATCCCTTTTTTTCAGAATGGATTATTTGCTCATGATTTTCTCT
  CTGCTGTTTGTGGCTTGCCAAGGAGCTCCAGAAACAGCAGTCTTAGGCGCTGAGCTCAGC
  GCGGTGGGTGAGAACGGCGGGGAGAAACCCACTCCCAGTCCACCCTGGCGGCTCCGCCGG
  TCCAAGCGCTGCTCCTGCTCGTCCCTGATGGATAAAGAGTGTGTCTACTTCTGCCACCTG
  GACATCATTTGGGTCAACACTCCCGAGCACGTTGTTCCGTATGGACTTGGAAGCCCTAGG
  TCCAAGAGAGCCTTGGAGAATTTACTTCCCACANAGGCAACAGACCGTGAGAATAGATGC
  CAATGTGCTAGCCAAAAGACAAGAAGTGCTGGAATTTTTGCCAAGCAGGAAAAGAACTCA
  GGGCTGAAGACATTATGGAGAAAGACTGGANNNTATCATAGANAGGAANAGACTGTTCCC
  AGCTTGNGAAAAAGTGTATNTATCAGCAGTTAGTGAGAGGAAGAAAATCAGAAGAAAGTC
  AGAGGACCACCTAGACAAACAGGTCGNAGACATGAGAACAGCGTCAATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001955 unedited
  TGGAGGAAGGGGCTCACAGGGCATGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGAC
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTTACTTGCATGTTTGTC
  TTTTGAGTGTGTTTTCAATTTGTGCATTCCTTAAAAAATCTTTGTGTGGACTTTGGATTT
  TCTCCCTGAAATGTGCCAGGCGCCTGATTCAAACACAAACACTCCCTTAAGACCTTCGTC
  AAAAACTCCACCCCTGTGTGGAATCTCCTTCCTCTCTCTCTCTCCGGAGAGATGCCACCC
  GAATTCAAATGTGACTGTGTGTTTCTGCTGAGAGGTCCATTGTCCTCCCCAGATGAAAGA
  AGAGACCAAAGCACTTACCACTGATGGAAGCCAGTGAATATGGTTGGGGGAACTCCTTAA
  CCTTTCTTGGAATGTTTTGAACGAGGACGCTGGTCCTTTGCCAGTCAGGAACCAGCAGAG
  GATGCTCCTGCTCTGATCCCAGCCAGGGTGGAGAGTGCAGATTCGGCCACAGGGCTCTCC
  GTGGAGGCTATGGCTTCAGACAGGCCCCGAAGGTCTGTCACCAATGTGCTCGGTTGTGGG
  TCACATAACGCTCTCTGGAGGGCTTGCCTTTCAGCTTGAGATCATGAAAAGATGATTTGA
  CGCTGTTTCTCATGGTCTCCGACCTGGTTTGTCTTAGGTGTTCCTCTGAACTTCTTCTGA
  TTTTTCTTCCTCTCACTAACTGCTGATAAATACACTTTTTCCCAAGCTTGGAACAGTCTT
  TTCCTTTCATATGATTATTCCAGTCTTTCTCCATATAGTCTCAGCCCTGATTTCTTTCCT
  GCTTGGCAAAAATCCANCCCTTCTGTCTTTTTGGCTACACATTGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001955
• Insert Size: 1550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001955.2, NP_001946.2
• RefSeq Size: 1334 bp
• RefSeq ORF: 639 bp
• Locus ID: 1906
• Cytogenetics: 6p24.1
• Domains: END
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Melanogenesis
• Gene Summary: 'This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).