EHHADH (NM_001966) Human Untagged Clone

CAT#: SC118906

EHHADH (untagged)-Human enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase (EHHADH), transcript variant 1


  "NM_001966" in other vectors (4)

Reconstitution Protocol

USD 1,460.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol EHHADH
Synonyms ECHD; FRTS3; L-PBE; LBFP; LBP; PBFE
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_001966 edited
GCGAATTCGGCACGAGACATGGCCGAGTATACGCGGCTGCACAACGCCTTGGCGCTAATC
CGCCTCCGAAACCCGCCGGTCAACGCGATCAGTACGACTTTACTCCGTGACATAAAAGAA
GGACTACAGAAAGCTGTAATAGACCATACAATAAAAGCCATTGTGATTTGTGGAGCAGAG
GGCAAATTTTCTGCAGGTGCTGATATTCGTGGCTTCAGTGCTCCTAGGACATTTGGCCTT
ACACTGGGACATGTAGTAGATGAAATACAGAGAAATGAGAAGCCCGTGGTGGCAGCAATC
CAAGGCATGGCTTTCGGAGGGGGACTAGAGCTGGCCCTGGGCTGTCACTATAGGATTGCC
CACGCAGAGGCTCAAGTTGGCTTACCAGAAGTTACACTGGGACTTCTCCCTGGTGCAAGA
GGAACCCAGCTTCTCCCCAGACTCACTGGAGTTCCTGCTGCACTTGACTTAATTACCTCA
GGAAGACGTATTTTAGCAGATGAAGCACTCAAGCTGGGCATTCTAGATAAAGTTGTAAAC
TCAGACCCGGTTGAAGAAGCAATCAGATTTGCTCAGAGAGTTTCAGATCAACCTCTAGAA
TCCCGTAGACTCTGCAACAAGCCAATTCAGAGCTTGCCCAACATGGACAGCATTTTTAGT
GAGGCCCTCTTGAAGATGCGGAGGCAGCACCCTGGGTGTCTTGCACAGGAGGCTTGTGTC
CGTGCAGTCCAGGCTGCTGTGCAGTATCCCTATGAAGTGGGCATCAAGAAGGAGGAGGAG
CTGTTTCTATATCTTTTGCAATCAGGGCAGGCTAGAGCCCTGCAATATGCTTTCTTCGCT
GAAAGGAAAGCAAATAAGTGGTCAACTCCCTCCGGAGCATCGTGGAAAACAGCATCAGCG
CGGCCTGTCTCCTCAGTTGGTGTTGTTGGCTTGGGAACAATGGGCCGAGGCATTGTCATT
TCTTTTGCAAGGGCCAGGATTCCTGTGATTGCTGTAGACTCGGACAAAAACCAGCTAGCA
ACTGCAAACAAGATGATAACCTCTGTCTTGGAAAAAGAAGCCTCCAAAATGCAACAGAGC
GGCCACCCTTGGTCAGGACCAAAACCCAGGTTAACTTCATCTGTGAAGGAGCTTGGTGGT
GTAGATTTAGTCATTGAAGCAGTATTTGAGGAAATGAGCCTGAAGAAGCAGGTCTTTGCT
GAACTCTCAGCTGTGTGCAAACCAGAAGCATTTTTGTGCACTAATACTTCAGCCCTGGAT
GTTGATGAGATTGCTTCTTCCACTGATCGTCCTCACTTGGTCATTGGCACCCACTTCTTT
TCGCCAGCTCATGTCATGAAGTTGTTAGAGGTTATTCCCAGCCAATACTCTTCCCCCACT
ACCATTGCCACTGTTATGAACTTATCAAAAAAGATTAAAAAGATTGGAGTCGTTGTAGGC
AACTGTTTTGGATTTGTGGGGAATCGAATGTTGAATCCTTACTACAATCAGGCATATTTC
TTGTTAGAAGAAGGCAGCAAACCAGAGGAGGTAGATCAGGTGCTGGAAGAGTTTGGTTTT
AAAATGGGACCTTTTAGAGTGTCTGATCTTGCTGGGTTGGATGTGGGCTGGAAATCTAGA
AAGGGGCAAGGTCTTACTGGACCTACATTGCTTCCAGGAACTCCTGCCCGAAAAAGGGGT
AATAGGAGGTACTGCCCAATTCCTGATGTGCTCTGTGAATTAGGACGATTTGGCCAGAAG
ACAGGTAAGGGTTGGTATCAATATGACAAGCCATTGGGTAGGATTCACAAACCTGATCCC
TGGCTTTCCAAATTCCTATCACGGTATAGAAAAACCCATCACATTGAACCACGTACCATT
AGCCAGGATGAGATCCTTGAACGCTGCTTATATTCACTTATCAATGAAGCATTCCGTATC
TTGGGAGAAGGGATAGCTGCTAGCCCAGAGCACATTGATGTTGTCTATTTACATGGATAT
GGATGGCCAAGGCACAAGGGCGGGCCCATGTTCTATGCTTCCACAGTTGGGTTGCCCACA
GTTCTAGAGAAATTGCAGAAATATTACAGGCAGAACCCTGATATTCCCCAACTGGAGCCA
AGTGACTATCTAAAAAAACTGGCTTCTCAGGGAAACCCTCCCCTGAAAGAATGGCAAAGC
TTGGCAGGCTCCCCTAGCAGTAAATTGTGATTCAGTCTTCCAGATTATGCCTCACATGCT
AGCATCAGGTAATGCTGACTGAATTTCAGTGAAATTAAATCAAAAATCCAAAGTAAGATT
GTTCTGAAATACAAAGCAAAATAAATAATCATTAGAATCTTCTGTGTAACGACTCTAATG
GTCAAATCTTTAGGAATGTGCTTCCTATGCCTCTGAATCTGTCCTTATCAGATAAATTCA
ATGCATGAACTTGTGTGAATATAATACCATAATAGCTAATGAAAGAGGCTCAAGCATAAG
TTGAGATTCTCAAATGCTTTTATCATTGGATAAATGTGTCATCAATTAATAAATGATAAA
TGCAGCTAAGT
>OriGene 5' read for NM_001966 unedited
AGCTCGNGATTTNGTAAACGCACTTCATATAGGGCGGACAAGCCATTCGGCACGAGAACA
TGGCCGAGTATACGCGGCTGCACAACGCCTTGGCGCTAATCCGCCTCCGAAACCCGCCGG
TCAACGCGATCAGTACGACTTTACTCCGTGACATAAAAGAAGGACTACAGAAAGCTGTAA
TAGACCATACAATAAAAGCCATTGTGATTTGTGGAGCATTTTTTAAATTTTCTGCAGGTG
CTGATATTCGTGGCTTCAGTGCTCCTAGGACATTTGGCCTTACACTGGGACATGTAGTAG
ATGAAATACAGAGAAATGAGAAGCCCGTGGTGGCAGCAATCCAAGGCATGGCTTTCGGAG
GGGGACTAGAGCTGGCCCTGGGCTGTCACTATAGGATTGCCCACGCAGAGGCTCAAGTTG
GCTTACCAGAAGTTACACTGGGACTTCTCCCTGGTGCAAGAGGAACCCAGCTTCTCCCCA
GACTCACTGGAGTTCCTGCTGCACTTGACTTAATTACCTCAGGAAGACGTATTTTAGCAG
ATGAAGCACTCAAGCTGGGCATTCTAGATAAAGTTGTAAACTCAGACCCGGTTGAAGAAG
CAATCAGATTTGCTCAGAGAGTTTCAGATCAACCTCTAGAATCCCGTAGACTCTGCAACA
AGCCAATTCAGAGCTTGCCCAACATGGACAGCATTTTTAGTGAGGCCCTCTTGAAGATGC
GGAGGCAGCACCCTGNGTGTCTTGCACAGGAGGCTTGTGTCCGTGCAGTCCAGGCTGCTG
TGCAGTATCCCTATGAAGTGGGCATNCAGAANGGAGAGGAGCTGTTTCTATATCTTTTGC
AATCAGGGCAGGCTAGAGCCCTGCCATATGCTTTTCTTCGCTGAAAAGGAAAGCAAATAG
TGGTCAACTTCCTCCGGAGCATCGTGGAA
>OriGene 3' read for NM_001966 unedited
TCCCCNNNCCAAAATTTTNCCCTNNNGCCCTCCCCNCCCCGGACTCTAGCCCGNGGCCCG
CCATNCTACATNCGGTTTTTTTTTTTTTTTTTTTTACTACTTTCCAGCAATCATTTTTAT
TGATGTCTTTAATTTCACAAACCAAATTATATAAAATTAAGTAGTACACATTTCAAACAT
AATAATTGTGCTCTGTACAATTCAAGGCACACTCGGATTCTTCGTTGGTTTTTTGCCCTG
CTTTTTAACAACGGTAAAACTTATACTCCCATTCACAGATTCTTCCCATCACGAACTTTA
ATCATTCCTATTCCGGCCTCACACGGTGAATACATTCGCGAAAGCTCATTTACACCACAA
TGAAGGATACCATCTTCAGACTTATTTATTCACTAACAACTTTATTGACATATATTTTAC
ATACCCTATCATTCACCCACTTAAAATACTTTCCTCTATGCCCTTTAACATATTGCCACG
CGACTACCACCACACTCCCTTTATACCATTCCCCCGACCCATAAATAAATCTTGCCCCTA
CAGATGAATTTCCCCCCCCCCCTTCATCCCTCCCAATTTTTCCCCCACCCCACCCCATGC
CCCGTAAGTTTCCCTGACTTCACCGCCTTCCCCTTTGTTCCCCCTCCTCCACCCAATCCA
CTGCCCTCACCTACTCTCCCTCCCCTCATACTTCCCCCCCCCCCCCCCCCCCTTCTCTCC
ACCCCTTGTTTCTCCCCTTCTTCTCCCCCCCTCTCTCTTCCTCCTCCTCCGCTACCCCAA
CTCTTCCACCCCCCCCCGTTTCCAAAAACCATTTTTCACCCCCTCACCACCTTCCGCCCC
CCCTCCCTCTTAGTCCCTTAACACCTATTCTCCCCCTCCTCCACCCGCATTTAAATTATT
ATTCGCCCCCACCCCCCCACATTTTTCATCTCCTTCACCCCACTTAACAACAATCTCACA
CATTTCTCACC
Restriction Sites NotI-NotI     
ACCN NM_001966
Insert Size 3520 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001966.2, NP_001957.2
RefSeq Size 3821 bp
RefSeq ORF 2172 bp
Locus ID 1962
Cytogenetics 3q27.2
Domains ECH, 3HCDH, 3HCDH_N
Protein Pathways beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, PPAR signaling pathway, Propanoate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation
Gene Summary 'The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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