Neutrophil Elastase (ELANE) (NM_001972) Human Untagged Clone

CAT#: SC118911

ELANE (untagged)-Human elastase, neutrophil expressed (ELANE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELANE
• Synonyms: ELA2; GE; HLE; HNE; NE; PMN-E; SCN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118911 sequence for NM_001972 edited (data generated by NextGen Sequencing)
  ATGACCCTCGGCCGCCGACTCGCGTGTCTTTTCCTCGCCTGTGTCCTGCCGGCCTTGCTG
  CTGGGGGGCACCGCGCTGGCCTCGGAGATTGTGGGGGGCCGGCGAGCGCGGCCCCACGCG
  TGGCCCTTCATGGTGTCCCTGCAGCTGCGCGGAGGCCACTTCTGCGGCGCCACCCTGATT
  GCGCCCAACTTCGTCATGTCGGCCGCGCACTGCGTGGCGAATGTAAACGTCCGCGCGGTG
  CGGGTGGTCCTGGGAGCCCATAACCTCTCGCGGCGGGAGCCCACCCGGCAGGTGTTCGCC
  GTGCAGCGCATCTTCGAAAACGGCTACGACCCCGTAAACTTGCTCAACGACATCGTGATT
  CTCCAGCTCAACGGGTCGGCCACCATCAACGCCAACGTGCAGGTGGCCCAGCTGCCGGCT
  CAGGGACGCCGCCTGGGCAACGGGGTGCAGTGCCTGGCCATGGGCTGGGGCCTTCTGGGC
  AGGAACCGTGGGATCGCCAGCGTCCTGCAGGAGCTCAACGTGACGGTGGTGACGTCCCTC
  TGCCGTCGCAGCAACGTCTGCACTCTCGTGAGGGGCCGGCAGGCCGGCGTCTGTTTCGGG
  GACTCCGGCAGCCCCTTGGTCTGCAACGGGCTAATCCACGGAATTGCCTCCTTCGTCCGG
  GGAGGCTGCGCCTCAGGGCTCTACCCCGATGCCTTTGCCCCGGTGGCACAGTTTGTAAAC
  TGGATCGACTCTATCATCCAACGCTCCGAGGACAACCCCTGTCCCCACCCCCGGGACCCG
  GACCCGGCCAGCAGGACCCACTGA
  
  Clone variation with respect to NM_001972.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001972 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGGGCGGGCACGGAGG
  GGCAGAGACCCCGGAGCCCCAGCCCCACCATGACCCTCGGCCGCCGACTCGCGTGTCTTT
  TCCTCGCCTGTGTCCTGCCGGCCTTGCTGCTGGGGGGCACCGCGCTGGCCTCGGAGATTG
  TGGGGGGCCGGCGAGCGCGGCCCCACGCGTGGCCCTTCATGGTGTCCCTGCAGCTGCGCG
  GAGGCCACTTCTGCGGCGCCACCCTGATTGCGCCCAACTTCGTCATGTCGGCCGCGCACT
  GCGTGGCGAATGTAAACGTCCGCGCGGTGCGGGTGGTCCTGGGAGCCCATAACCTCTCGC
  GGCGGGAGCCCACCCGGCAGGTGTTCGCCGTGCAGCGCATCTTCGAAAACGGCTACGACC
  CCGTAAACTTGCTCAACGACATCGTGATTCTCCAGCTCAACGGGTCGGCCACCATCAACG
  CCAACGTGCAGGTGGCCCAGCTGCCGGCTCAGGGACGCCGCCTGGGCAACGGNGTGCAGT
  GCCTGGCCATGGGCTGNNGCCTTTCTGGCAGGAACCGTGGGATCNGCAGCGTCCTGCAGG
  AGCTCAACGTGACGGTGGTGACGTCCCTCTGCCGTCGCAGCAACGTCTGCACTCTCGTGA
  GGGGCCCNGCAGCCCGCCGTCTGNTTCGGGGACTCCNGCAGCCCCTGGGTCTGNCACGGG
  CTAATCCACGGAANTGGCTCCTTCGTCCGGGGGAGGCTGCGCCTCCAGGCCTCTACCCCG
  ATGCCCTTGCCCCCGTGGNCACACGTTGTAAACTGGATCGACTTCTATATCCAACGCTCC
  GGAGACAACCCCTGTCCCCACCCCCGGAACCCGGACCCGGCCAAAGGACCACTTGAAAGG
  TCTGCCCGGGTACCTAAGTTGGCCCAACCCACCTCTCAAGATTGGACCATAACATTTTGG
  TTTGTAAAAAAAAAAAAAAAAAAACATCATCCTTAATGGGGCCGGGCTTAATTGTTCCTG
  ACATACCCGGGGGGACCTTGGACTCTCCAGGGCTCCGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001972 unedited
  GGCCCGGGGGGNNNNCNNNNNNNTTTANNNNNNNCGGTNTTACTCTGANCCGCGGNCCGC
  ATACNAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTACAAAACTAAAAAGTTTATTGT
  GCCAGATGCTGGAGAGTGTGGGTGTGGGCAGCTGAGGTGACCCGGGCAGCCCTTCTCAGT
  GGGTCCTGCTGGCCGGGTCCGGGTCCCGGGGGTGGGGACAGGGGTTGTCCTCGGAGCGTT
  GGATGATAGAGTCGATCCAGTTTACAAACTGTGCCACCGGGGCAAAGGCATCGGGGTAGA
  GCCCTGAGGCGCAGCCTCCCCGGACGAAGGAGGCAATTCCGTGGATTAGCCCGTTGCAGA
  CCAAGGGGCTGCCGGAGTCCCCGAAACAGACGCCGGCCTGCCGGCCCCTCACGAGAGTGC
  AGACGTTGCTTGCGACGGCAGAGGGACGTTACCACCGTCACGTTGAGCTCCTGCAGGAAC
  CTGGCGAACCCACGGTTCCTGCCCAAAAGCCCCCACCCATGGGCCCGGCCCTTGCCCCCC
  TTGCCCAAGGCGGGGTCCCTTAACCGTACATTTGGGCCCCCCTGCACCTTTGGGGTTTGA
  AGGGGGCCCCACCCCTTCTCATTTCTGAAAAAAAAATAATTTTTTTAAAAAATTTCGGGG
  GGGCTAACCCTTTTTAAAAAATCCCTTTGTCGGGAAAAAACCCCCCGCGGTGGGCCCCCC
  CCCCCAGAAGGGTTTGGTGGTTAAAAAAAACACCCCCCCCTCGTGGGGGGGTTTTTATTT
  CTTTCCCCCCGAGGGGCGGGCGGCCAAAAAAAAAAAAATAGGGCACCCCCAACGGGGGGG
  GTGGCCCCAAATATAACGTTTTCCCCTCCCTCTTTTGTGGCGGCACCACAAAATATAGCA
  CCCTCCCCGTGGGGGCGCTTCTCCCTTCTCCCCCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001972
• Insert Size: 1050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001972.2, NP_001963.1
• RefSeq Size: 938 bp
• RefSeq ORF: 804 bp
• Locus ID: 1991
• Cytogenetics: 19p13.3
• Domains: Tryp_SPc
• Protein Families: Protease, Transmembrane
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: 'Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]'