ENO3 (NM_001976) Human Untagged Clone

CAT#: SC118914

ENO3 (untagged)-Human enolase 3 (beta, muscle) (ENO3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENO3
• Synonyms: GSD13; MSE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118914 sequence for NM_001976 edited (data generated by NextGen Sequencing)
  ATGGCCATGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAGGGGCAACCCCACGGTG
  GAGGTGGACCTGCACACGGCCAAGGGCCGATTCCGAGCAGCTGTGCCCAGTGGGGCTTCC
  ACGGGTATCTATGAGGCTCTGGAACTAAGAGACGGAGACAAAGGCCGCTACCTGGGGAAA
  GGAGTCCTGAAGGCTGTGGAGAACATCAACAGTACTCTGGGCCCTGCTCTGCTGCAAAAG
  AAACTAAGCGTTGCGGATCAAGAAAAAGTTGACAAATTTATGATTGAGCTAGATGGGACC
  GAGAATAAGTCCAAGTTTGGGGCCAATGCCATCCTGGGCGTGTCCTTGGCCGTGTGTAAG
  GCGGGAGCAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCAGATCTCGCTGGGAAC
  CCTGACCTCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGGGGGCTCCCATGCTGGA
  AACAAGCTGGCCATGCAGGAGTTCATGATTCTGCCTGTGGGAGCCAGCTCCTTCAAGGAA
  GCCATGCGCATTGGCGCCGAGGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTAT
  GGGAAGGATGCCACCAATGTGGGTGATGAAGGTGGCTTCGCACCCAACATCCTGGAGAAC
  AATGAGGCCCTGGAGCTGCTGAAGACGGCCATCCAGGCGGCTGGTTACCCAGACAAGGTG
  GTGATCGGCATGGATGTGGCAGCATCTGAGTTCTATCGCAATGGGAAGTACGATCTTGAC
  TTCAAGTCGCCTGATGATCCCGCACGGCACATCACTGGGGAGAAGCTCGGAGAGCTGTAT
  AAGAGCTTTATCAAGAACTATCCTGTGGTCTCCATCGAAGACCCCTTTGACCAGGATGAC
  TGGGCCACTTGGACCTCCTTCCTCTCGGGGGTGAACATCCAGATTGTGGGGGATGACTTG
  ACAGTCACCAACCCCAAGAGGATTGCCCAGGCCGTTGAGAAGAAGGCCTGCAACTGTCTG
  CTGCTGAAGGTCAACCAGATCGGCTCGGTGACCGAATCGATCCAGGCGTGCAAACTGGCT
  CAGTCTAATGGCTGGGGGGTGATGGTGAGCCACCGCTCTGGGGAGACTGAGGACACATTC
  ATTGCTGACCTTGTGGTGGGGCTCTGCACAGGACAGATCAAGACTGGCGCCCCCTGCCGC
  TCGGAGCGTCTGGCCAAATACAACCAACTCATGAGGATCGAGGAGGCTCTTGGGGACAAG
  GCAATCTTTGCTGGACGCAAGTTCCGTAACCCGAAGGCCAAGTGA
  
  Clone variation with respect to NM_001976.4
  212 a=>g;254 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001976 unedited
  TGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCTACACTCACTCACAC
  CTCCTGTCCTGCAGCCATGGCCATGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAG
  GGGCAACCCCACGGTGGAGGTGGACCTGCACACGGCCAAGGGCCGATTCCGAGCAGCTGT
  GCCCAGTGGGGCTTCCACGGGTATCTATGAGGCTCTGGAACTAAGAGACGGAGACAAAGG
  CCGCTACCTGGGGAAAGGAGTCCTGAAGGCTGTGGAGAACATCAACAGTACTCTGGGCCC
  TGCTCTGCTGCAAAAGAAACTAAGCGTTGCGGATCAAGAAAAAGTTGACAAATTTATGAT
  TGAGCTAGATGGGACCGAGAATAAGTCCAAGTTTGGGGCCAATGCCATCCTGGGCGTGTC
  CTTGGCCGTGTGTAAGGCGGGAGCAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGC
  AGATCTCGCTGGGAACCCTGACCTCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGG
  GGGCTCCCATGCTGGAAACAAGCTGGCCATGCAGGAGTTCATGATTCTGCCTGTGGGAGC
  CAGCTCCTTCAAGGAAGCCATGCGCATTGGCGCCGAGGTCTACCACCACCTCAAGGGGGT
  CATCAAGGCCAAGTATGGGAAGGATGCCACCAATGTGGGTGATGAAGGTGGCTTCGCACC
  CAACATCCTGGAGAACATGAGGCCCTGNAGCTGCTGAAGACGGCCATCCAGGCGGCTGGT
  TACCCAGACAAGGTGGTGATNCGCATGGGATGTGCAGCATCTGAGTTCTATCGCAATGGG
  AGTACGATCTTGACTTCAGTCGCCTGATGATCCCGCACGCACATACTGGGGAGAGCTCGG
  AGACTGNATAGAGCTTATCAGAACATNCTGTGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001976 unedited
  CTGGCCCAGTGTTNTATTTCAGGAAGCAGTCTGGNAGNACTGGGTCTGTCCAGTGGAGTC
  CTGGAGCCTCCAGCTTCTCACTTGGCCTTCGGGTTACGGAACTTGCGTCCAGCAAAGATT
  GCCTTGTCCCCAAGAGCCTCCTCGATCCTCATGAGTTGGTTGTATTTGGCCAGACGCTCC
  GAGCGGCAGGGGGCGCCAGTCTTGATCTGTCCTGTGCAGAGCCCCACCACAAGGTCAGCA
  ATGAATGTGTCCTCAGTCTCCCCAGAGCGGTGGCTCACCATCACCCCCCAGCCATTAGAC
  TGAGCCAGTTTGCACGCCTGGATCGATTCGGTCACCGAGCCGATCTGGTTGACCTTCAGC
  AGCAGACAGTTGCAGGCCTTCTTCTCAACGGCCTGGGCAATCCTCTTGGGGTTGGTGACT
  GTCAAGTCATCCCCCACAATCTGGATGTTCACCCCCGAGAGGAAGGAGGTCCAAGTGGCC
  CAGTCATCCTGGTCAAAGGGGTCTTCGATGGAGACCACANGATAGTTCTTGATAAAGCTC
  TTATACAGCTCTCCGAGCTTCTCCCCAGTGATGTGCCGTGCGGGATCATCAGGCGACTTG
  AAGTCAAGATCGTACTTNCCATTGCGATAGAACTCAGATGCTGCCACATCCATGCCGATC
  ACCACCTTGTCTGGGTAACCAGCCGCCTGGATGGCCGTCTCTAACAGCTCCAAGGCCTAA
  TTGGTTTCCAAGATGCTGGGGGCAAAACCCCCTTAATACCCACATTGGGGGCATCCCTCC
  CTACCTCGCCCTTGAGACCCCCCTGAGGGGGGGGAGTACCTTCGGGCCAATGCGAATGGG
  CTTCTTGCAAGACCTGCGCTCCACGGCGCAACCATCACCCCCGCTTGCCCGCTTGGCCCC
  AACTGGCCCCCCCTCTACACCAATGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001976
• Insert Size: 1560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001976.2, NP_001967.1
• RefSeq Size: 1494 bp
• RefSeq ORF: 1305 bp
• Locus ID: 2027
• Cytogenetics: 17p13.2
• Domains: enolase
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
• Gene Summary: 'This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same protein.