ENO3 (NM_001976) Human Untagged Clone

CAT#: SC118914

ENO3 (untagged)-Human enolase 3 (beta, muscle) (ENO3), transcript variant 1


  "NM_001976" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ENO3
Synonyms GSD13; MSE
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118914 sequence for NM_001976 edited (data generated by NextGen Sequencing)
ATGGCCATGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAGGGGCAACCCCACGGTG
GAGGTGGACCTGCACACGGCCAAGGGCCGATTCCGAGCAGCTGTGCCCAGTGGGGCTTCC
ACGGGTATCTATGAGGCTCTGGAACTAAGAGACGGAGACAAAGGCCGCTACCTGGGGAAA
GGAGTCCTGAAGGCTGTGGAGAACATCAACAGTACTCTGGGCCCTGCTCTGCTGCAAAAG
AAACTAAGCGTTGCGGATCAAGAAAAAGTTGACAAATTTATGATTGAGCTAGATGGGACC
GAGAATAAGTCCAAGTTTGGGGCCAATGCCATCCTGGGCGTGTCCTTGGCCGTGTGTAAG
GCGGGAGCAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCAGATCTCGCTGGGAAC
CCTGACCTCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGGGGGCTCCCATGCTGGA
AACAAGCTGGCCATGCAGGAGTTCATGATTCTGCCTGTGGGAGCCAGCTCCTTCAAGGAA
GCCATGCGCATTGGCGCCGAGGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTAT
GGGAAGGATGCCACCAATGTGGGTGATGAAGGTGGCTTCGCACCCAACATCCTGGAGAAC
AATGAGGCCCTGGAGCTGCTGAAGACGGCCATCCAGGCGGCTGGTTACCCAGACAAGGTG
GTGATCGGCATGGATGTGGCAGCATCTGAGTTCTATCGCAATGGGAAGTACGATCTTGAC
TTCAAGTCGCCTGATGATCCCGCACGGCACATCACTGGGGAGAAGCTCGGAGAGCTGTAT
AAGAGCTTTATCAAGAACTATCCTGTGGTCTCCATCGAAGACCCCTTTGACCAGGATGAC
TGGGCCACTTGGACCTCCTTCCTCTCGGGGGTGAACATCCAGATTGTGGGGGATGACTTG
ACAGTCACCAACCCCAAGAGGATTGCCCAGGCCGTTGAGAAGAAGGCCTGCAACTGTCTG
CTGCTGAAGGTCAACCAGATCGGCTCGGTGACCGAATCGATCCAGGCGTGCAAACTGGCT
CAGTCTAATGGCTGGGGGGTGATGGTGAGCCACCGCTCTGGGGAGACTGAGGACACATTC
ATTGCTGACCTTGTGGTGGGGCTCTGCACAGGACAGATCAAGACTGGCGCCCCCTGCCGC
TCGGAGCGTCTGGCCAAATACAACCAACTCATGAGGATCGAGGAGGCTCTTGGGGACAAG
GCAATCTTTGCTGGACGCAAGTTCCGTAACCCGAAGGCCAAGTGA

Clone variation with respect to NM_001976.4
212 a=>g;254 t=>c
>OriGene 5' read for NM_001976 unedited
TGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCTACACTCACTCACAC
CTCCTGTCCTGCAGCCATGGCCATGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAG
GGGCAACCCCACGGTGGAGGTGGACCTGCACACGGCCAAGGGCCGATTCCGAGCAGCTGT
GCCCAGTGGGGCTTCCACGGGTATCTATGAGGCTCTGGAACTAAGAGACGGAGACAAAGG
CCGCTACCTGGGGAAAGGAGTCCTGAAGGCTGTGGAGAACATCAACAGTACTCTGGGCCC
TGCTCTGCTGCAAAAGAAACTAAGCGTTGCGGATCAAGAAAAAGTTGACAAATTTATGAT
TGAGCTAGATGGGACCGAGAATAAGTCCAAGTTTGGGGCCAATGCCATCCTGGGCGTGTC
CTTGGCCGTGTGTAAGGCGGGAGCAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGC
AGATCTCGCTGGGAACCCTGACCTCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGG
GGGCTCCCATGCTGGAAACAAGCTGGCCATGCAGGAGTTCATGATTCTGCCTGTGGGAGC
CAGCTCCTTCAAGGAAGCCATGCGCATTGGCGCCGAGGTCTACCACCACCTCAAGGGGGT
CATCAAGGCCAAGTATGGGAAGGATGCCACCAATGTGGGTGATGAAGGTGGCTTCGCACC
CAACATCCTGGAGAACATGAGGCCCTGNAGCTGCTGAAGACGGCCATCCAGGCGGCTGGT
TACCCAGACAAGGTGGTGATNCGCATGGGATGTGCAGCATCTGAGTTCTATCGCAATGGG
AGTACGATCTTGACTTCAGTCGCCTGATGATCCCGCACGCACATACTGGGGAGAGCTCGG
AGACTGNATAGAGCTTATCAGAACATNCTGTGTC
>OriGene 3' read for NM_001976 unedited
CTGGCCCAGTGTTNTATTTCAGGAAGCAGTCTGGNAGNACTGGGTCTGTCCAGTGGAGTC
CTGGAGCCTCCAGCTTCTCACTTGGCCTTCGGGTTACGGAACTTGCGTCCAGCAAAGATT
GCCTTGTCCCCAAGAGCCTCCTCGATCCTCATGAGTTGGTTGTATTTGGCCAGACGCTCC
GAGCGGCAGGGGGCGCCAGTCTTGATCTGTCCTGTGCAGAGCCCCACCACAAGGTCAGCA
ATGAATGTGTCCTCAGTCTCCCCAGAGCGGTGGCTCACCATCACCCCCCAGCCATTAGAC
TGAGCCAGTTTGCACGCCTGGATCGATTCGGTCACCGAGCCGATCTGGTTGACCTTCAGC
AGCAGACAGTTGCAGGCCTTCTTCTCAACGGCCTGGGCAATCCTCTTGGGGTTGGTGACT
GTCAAGTCATCCCCCACAATCTGGATGTTCACCCCCGAGAGGAAGGAGGTCCAAGTGGCC
CAGTCATCCTGGTCAAAGGGGTCTTCGATGGAGACCACANGATAGTTCTTGATAAAGCTC
TTATACAGCTCTCCGAGCTTCTCCCCAGTGATGTGCCGTGCGGGATCATCAGGCGACTTG
AAGTCAAGATCGTACTTNCCATTGCGATAGAACTCAGATGCTGCCACATCCATGCCGATC
ACCACCTTGTCTGGGTAACCAGCCGCCTGGATGGCCGTCTCTAACAGCTCCAAGGCCTAA
TTGGTTTCCAAGATGCTGGGGGCAAAACCCCCTTAATACCCACATTGGGGGCATCCCTCC
CTACCTCGCCCTTGAGACCCCCCTGAGGGGGGGGAGTACCTTCGGGCCAATGCGAATGGG
CTTCTTGCAAGACCTGCGCTCCACGGCGCAACCATCACCCCCGCTTGCCCGCTTGGCCCC
AACTGGCCCCCCCTCTACACCAATGCC
Restriction Sites NotI-NotI     
ACCN NM_001976
Insert Size 1560 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001976.2, NP_001967.1
RefSeq Size 1494 bp
RefSeq ORF 1305 bp
Locus ID 2027
Cytogenetics 17p13.2
Domains enolase
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
Gene Summary 'This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same protein.

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