EZH1 (NM_001991) Human Untagged Clone

CAT#: SC118923

EZH1 (untagged)-Human enhancer of zeste homolog 1 (Drosophila) (EZH1)


  "NM_001991" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol EZH1
Synonyms KMT6B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001991, the custom clone sequence may differ by one or more nucleotides


ATGGAAATACCAAATCCCCCTACCTCCAAATGTATCACTTACTGGAAAAGAAAAGTGAAATCTGAATACA
TGCGACTTCGACAACTTAAACGGCTTCAGGCAAATATGGGTGCAAAGGCTTTGTATGTGGCAAATTTTGC
AAAGGTTCAAGAAAAAACCCAGATCCTCAATGAAGAATGGAAGAAGCTTCGTGTCCAACCTGTTCAGTCA
ATGAAGCCTGTGAGTGGACACCCTTTTCTCAAAAAGTGTACCATAGAGAGCATTTTCCCGGGATTTGCAA
GCCAACATATGTTAATGAGGTCACTGAACACAGTTGCATTGGTTCCCATCATGTATTCCTGGTCCCCTCT
CCAACAGAACTTTATGGTAGAAGATGAGACGGTTTTGTGCAATATTCCCTACATGGGAGATGAAGTGAAA
GAAGAAGATGAGACTTTTATTGAGGAGCTGATCAATAACTATGATGGGAAAGTCCATGGTGAAGAAGAGA
TGATCCCTGGATCCGTTCTGATTAGTGATGCTGTTTTTCTGGAGTTGGTCGATGCCCTGAATCAGTACTC
AGATGAGGAGGAGGAAGGGCACAATGACACCTCAGATGGAAAGCAGGATGACAGCAAAGAAGATCTGCCA
GTAACAAGAAAGAGAAAGCGACATGCTATTGAAGGCAACAAAAAGAGTTCCAAGAAACAGTTCCCAAATG
ACATGATCTTCAGTGCAATTGCCTCAATGTTCCCTGAGAATGGTGTCCCAGATGACATGAAGGAGAGGTA
TCGAGAACTAACAGAGATGTCAGACCCCAATGCACTTCCCCCTCAGTGCACACCCAACATCGATGGCCCC
AATGCCAAGTCTGTGCAGCGGGAGCAATCTCTGCACTCCTTCCACACACTTTTTTGCCGGCGCTGCTTTA
AATACGACTGCTTCCTTCACCCTTTTCATGCCACCCCTAATGTATATAAACGCAAGAATAAAGAAATCAA
GATTGAACCAGAACCATGTGGCACAGACTGCTTCCTTTTGCTGGAAGGAGCAAAGGAGTATGCCATGCTC
CACAACCCCCGCTCCAAGTGCTCTGGTCGTCGCCGGAGAAGGCACCACATAGTCAGTGCTTCCTGCTCCA
ATGCCTCAGCCTCTGCTGTGGCTGAGACTAAAGAAGGAGACAGTGACAGGGACACAGGCAATGACTGGGC
CTCCAGTTCTTCAGAGGCTAACTCTCGCTGTCAGACTCCCACAAAACAGAAGGCTAGTCCAGCCCCACCT
CAACTCTGCGTAGTGGAAGCACCCTCGGAGCCTGTGGAATGGACTGGGGCTGAAGAATCTCTTTTTCGAG
TCTTCCATGGCACCTACTTCAACAACTTCTGTTCAATAGCCAGGCTTCTGGGGACCAAGACGTGCAAGCA
GGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTGAAGCTGCCAACAGATGAGCTCATGAACCCCTCA
CAGAAGAAGAAAAGAAAGCACAGATTGTGGGCTGCACACTGCAGGAAGATTCAGCTGAAGAAAGATAACT
CTTCCACACAAGTGTACAACTACCAACCCTGCGACCACCCAGACCGCCCCTGTGACAGCACCTGCCCCTG
CATCATGACTCAGAATTTCTGTGAGAAGTTCTGCCAGTGCAACCCAGACTGTCAGAATCGTTTCCCTGGC
TGTCGCTGTAAGACCCAGTGCAATACCAAGCAATGTCCTTGCTATCTGGCAGTGCGAGAATGTGACCCTG
ACCTGTGTCTCACCTGTGGGGCCTCAGAGCACTGGGACTGCAAGGTGGTTTCCTGTAAAAACTGCAGCAT
CCAGCGTGGACTTAAGAAGCACCTGCTGCTGGCCCCCTCTGATGTGGCCGGATGGGGCACCTTCATAAAG
GAGTCTGTGCAGAAGAACGAATTCATTTCTGAATACTGTGGTGAGCTCATCTCTCAGGATGAGGCTGATC
GACGCGGAAAGGTCTATGACAAATACATGTCCAGCTTCCTCTTCAACCTCAATAATGATTTTGTAGTGGA
TGCTACTCGGAAAGGAAACAAAATTCGATTTGCAAATCATTCAGTGAATCCCAACTGTTATGCCAAAGTG
GTCATGGTGAATGGAGACCATCGGATTGGGATCTTTGCCAAGAGGGCAATTCAAGCTGGCGAAGAGCTCT
TCTTTGATTACAGGTACAGCCAAGCTGATGCTCTCAAGTACGTGGGGATCGAGAGGGAGACCGACGTCCT
TTAG


Restriction Sites NotI-NotI     
ACCN NM_001991
Insert Size 4470 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001991.3, NP_001982.2
RefSeq Size 4654 bp
RefSeq ORF 2244 bp
Locus ID 2145
Cytogenetics 17q21.2
Domains myb_DNA-binding, SET
Protein Families Druggable Genome, Transcription Factors
Gene Summary 'EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.