Neutrophil defensin 4 (DEFA4) (NM_001925) Human Untagged Clone

CAT#: SC118948

DEFA4 (untagged)-Human defensin, alpha 4, corticostatin (DEFA4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DEFA4
• Synonyms: DEF4; HNP-4; HP-4; HP4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118948 sequence for NM_001925 edited (data generated by NextGen Sequencing)
  ATGAGGATTATCGCCCTCCTCGCTGCTATTCTCTTGGTAGCCCTCCAGGTCCGGGCAGGC
  CCACTCCAGGCAAGAGGTGATGAGGCTCCAGGCCAGGAGCAGCGTGGGCCAGAAGACCAG
  GACATATCTATTTCCTTTGCATGGGATAAAAGCTCTGCTCTTCAGGTTTCAGGCTCAACA
  AGGGGCATGGTCTGCTCTTGCAGATTAGTATTCTGCCGGCGAACAGAACTTCGTGTTGGG
  AACTGCCTCATTGGCGGTGTGAGTTTCACATACTGCTGCACGCGTGTCGATTAA
  
  Clone variation with respect to NM_001925.1
  255 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001925 unedited
  TCAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCTGGAACACAGA
  CTGCTGTCTGCCCTCTCTGCTCGCCCTGCCTAGCTTGAGGATCTGTCACCCCAGCCATGA
  GGATTATCGCCCTCCTCGCTGCTATTCTCTTGGTAGCCCTCCAGGTCCGGGCAGGCCCAC
  TCCAGGCAAGAGGTGATGAGGCTCCAGGCCAGGAGCAGCGTGGGCCAGAAGACCAGGACA
  TATCTATTTCCTTTGCATGGGATAAAAGCTCTGCTCTTCAGGTTTCAGGCTCAACAAGGG
  GCATGGTCTGCTCTTGCAGATTAGTATTCTGCCGGCGAACAGAACTTCGTGTTGGGAACT
  GCCTCATTGGCGGTGTGAGTTTCACATACTGCTGCACGCGTGTCGATTAACATTCTGCTG
  TCCAAGAGAATGTCATGCTGGGAACGCCATCATCGGTGGTGTTAGCTTCACATGCTTCTG
  CAGCTGAGCTTGCAGAATAGAGAAAAATGAGCTCATAATTTGCTTTGAGAGCTACAGGAA
  ATGGTTGTTTCTCCTATACTTTGTCCTTAACATCTTTCTTGATCCTAAATATATATCTCG
  TAACAAGATGAANNANANAAAANAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCG
  CGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGC
  CTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAA
  GTTGCATCATTNTGTCTGACTAGGTGTCCTTCTATAATATATAGGGGTGAGGGGGTGGGG
  TATTGGGACCAAGGGGCCAGNTGGGAAAAACACCTGTAGGCCTTGCGGTTCTATGGGAAC
  CAGCTGGATGCAGTGCCCAATCTGGCTCATGCATCTCGCTCCTGGGTCAGCGATCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001925 unedited
  NGGGCNACNNNGCCCCCTTANTTTGGGCGATTTGNACCGGGCCGCATACTAGATCGAGTN
  NTTTTTTTTTTAGTTTTTTTTTTTTTTCATCTTGTTACGAGAAATATATTTAGGATCAAG
  AAAGATGTTAAGGACAAAGTATAGGGAAAAACAACCATTTCCTGTAGCTCTCAAAGCAAA
  TTATGAGCTCATTTTTCTCTATTCTGCAAGCTCAGCTGCAAAAGCATGTGAAGCTAACAC
  CACCGATGATGGCGTTCCCAGCATGACATTCTCTTGGACAGCAGAATGTTAATCGACACG
  CGTGCAGCAGTATGTGAAACTCACACCGCCAATGAGGCAGTTCCCAACACGAAGTTCTGT
  TCGCCGGCAGAATACTAATCTGCAAGAGCAGACCATGCCCCTTGTTGAGCCTGAAACCTG
  AAGAGCAGAGCTTTTATCCCATGCAAAGGAAATAGATATGTCCTGGTCTTCTGGCCCACG
  CTGCTCCTGGCCTGGAGCCTCATCACCTCTTGCCTGAAGTGGGCCTGCCCGCACCTGGAG
  GGCTACCAAGAGAATAGCAGCGAGGAGGGCGATTATCCTCATGGCTGGGTGACAGATCCT
  CAAGCTAGGCAGGCCGAGCAGAAAGGCCAAACCGCAGTCCTGTGTTCCAGCTCGTGCCCG
  AATCCCGGCCGGCCTATGTGAGTCGTATACAAAATTTTGACGGTCACTAACGAGCTTTGT
  TAAATAGACCTCCACCGTACCGCCTACCGCCATTTTGCGTAACGGGGCGGGGTAATACCA
  AATTTTGAAAGTCCCGTGATTTTGGTGCCAAACAACTCCCATGCCGAAATGGGGGGAAAC
  TGGAAACCCCGGGAGTAAACCGTTCCCCGCCCTGGGGGACTGCCAAACCGTTACCCGGGG
  AAAGCCAGATAAAACCTAAATTCCTGCCAAATGGAAACCCCTAAGCCATTTCTGGGAATA
  AGCCAGGGGCATTTACGGTTTGAGCAAAAGGGGGGCCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001925
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001925.1, NP_001916.1
• RefSeq Size: 542 bp
• RefSeq ORF: 294 bp
• Locus ID: 1669
• Cytogenetics: 8p23.1
• Domains: Defensin_propep, DEFSN, defensins
• Protein Families: Secreted Protein
• Gene Summary: 'Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from other genes of this family by an extra 83-base segment that is apparently the result of a recent duplication within the coding region. The protein encoded by this gene, defensin, alpha 4, is found in the neutrophils; it exhibits corticostatic activity and inhibits corticotropin stimulated corticosterone production. [provided by RefSeq, Oct 2014]'