Alpha B Crystallin (CRYAB) (NM_001885) Human Untagged Clone

CAT#: SC118965

CRYAB (untagged)-Human crystallin, alpha B (CRYAB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRYAB
• Synonyms: CMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118965 sequence for NM_001885 edited (data generated by NextGen Sequencing)
  ATGGACATCGCCATCCACCACCCCTGGATCCGCCGCCCCTTCTTTCCTTTCCACTCCCCC
  AGCCGCCTCTTTGACCAGTTCTTCGGAGAGCACCTGTTGGAGTCTGATCTTTTCCCGACG
  TCTACTTCCCTGAGTCCCTTCTACCTTCGGCCACCCTCCTTCCTGCGGGCACCCAGCTGG
  TTTGACACTGGACTCTCAGAGATGCGCCTGGAGAAGGACAGGTTCTCTGTCAACCTGGAT
  GTGAAGCACTTCTCCCCAGAGGAACTCAAAGTTAAGGTGTTGGGAGATGTGATTGAGGTG
  CATGGAAAACATGAAGAGCGCCAGGATGAACATGGTTTCATCTCCAGGGAGTTCCACAGG
  AAATACCGGATCCCAGCTGATGTAGACCCTCTCACCATTACTTCATCCCTGTCATCTGAT
  GGGGTCCTCACTGTGAATGGACCAAGGAAACAGGTCTCTGGCCCTGAGCGCACCATTCCC
  ATCACCCGTGAAGAGAAGCCTGCTGTCACCGCAGCCCCCAAGAAATAG
  
  Clone variation with respect to NM_001885.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001885 unedited
  GTGCACAATTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGTTTCTTAGCT
  CAGTGAGTACTGGGTATGTGTCACATTGCCAAATCCCGGATCACAAGTCTCCATGAACTG
  CTGGTGAGCTAGGATAATAAAACCCCTGACATCACCATTCCAGAAGCTTCACAAGACTGC
  ATATATAAGGGGCTGGCTGTAGCTGCAGCTGAAGGAGCTGACCAGCCAGCTGACCCCTCA
  CACTCACCTAGCCACCATGGACATCGCCATCCACCACCCCTGGATCCGCCGCCCCTTCTT
  TCCTTTCCACTCCCCCAGCCGCCTCTTTGACCAGTTCTTCGGAGAGCACCTGTTGGAGTC
  TGATCTTTTCCCGACGTCTACTTCCCTGAGTCCCTTCTACCTTCGGCCACCCTCCTTCCT
  GCGGGCACCCAGCTGGTTTGACACTGGACTCTCAGAGATGCGCCTGGAGAAGGACAGGTT
  CTCTGTCAACCTGGATGTGAAGCACTTCTCCCCAGAGGAACTCAAAGTTAAGGTGTTGGG
  AGATGTGATTGAGGTGCATGGAAAACATGAAGAGCGCCAGGATGAACATGGTTTCATCTC
  CAGGGAGTTCCACAGGAAATACCGGATCCCAGCTGATGTAGACCCTCTCACCATTACTTC
  ATCCCTGTCATCTGATGGGGTCCTCACTGTGAATGGACCCAAGAAACAGGTCTCTGGCCC
  TGAGCGCACCATTCCCATCACCCGTGAAGAGAGCCTGCTGTCACCGCAGCCCCCAAGAAT
  AGATGCCCTTCCTCGATNGCCATTTTTAAACCAGAAAGTTCCCCCACAGTGAATGAAGTC
  CTGTGACTAATGCTGAAGCTTATTATGCTAAAGGCNGCCCCAATATCAAGCTATAAAAAT
  CCATCACAACAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001885 unedited
  CGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTGTTGCTGAATGATATTTT
  ATTAGCTTGATAATTTGGGCCTGCCCTTAGCATTAATAAGCTTCAGCACTAGTCACAAGA
  CTTTCATTCACTGGTGGGGAAACTTTCTTGTTTTAAAAAATGCAATTCAAGAAAGGGCAT
  CTATTTCTTGGGGGCTGCGGTGACAGCAGGCTTCTCTTCACGGGTGATGGGAATGGTGCG
  CTCAGGGCCAGAGACCTGTTTCCTTGGTCCATTCACAGTGAGGACCCCATCAGATGACAG
  GGATGAAGTAATGGTGAGAGGGTCTACATCAGCTGGGATCCGGTATTTCCTGTGGAACTC
  CCTGGAGATGAAACCATGTTCATCCTGGCGCTCTTCATGTTTTCCATGCACCTCAATCAC
  ATCTCCCAACACCTTAACTTTGAGTTCCTCTGGGGAGAAGTGCTTCACATCCAGGTTGAC
  AGAGAACCTGTCCTTCTCCAGGCGCATCTCTGAGAGTCCAGTGTCAAACCAGCTGGGTGC
  CCGCAGGAAGGAGGGTGGCCGAAGGTAGAAGGGACTCAGGGAAGTAGACGTCGGGAAAAG
  ATCAGACTCCAACAGGTGCTCTCCGAAGAACTGGTCAAAGAGGCGGCTGGGGGAGTGGAA
  AGGAAAGAAGGGGCGGCGGATCCAGGGGTGGTGGATGGCGATGTCCATGGTGGCTAGGTG
  AGTGTGAGGGGTCAGCTGGCTGGTCAGCTCCTTCAGCTGCAGCTACAGCCAGCCCCTTAT
  ATATGCAGTCTTGTGAAGCTTCTGGAATGGTGATGTCAGGGGTTTTATTATCCTAGCTCA
  CAGCAGTTCATGGAGACTTGTGATCCCNGGANNNTGCATGTGACACATACCAGTACTNAC
  TGAGCCTAGAAACCTCTGCCGNAATCGCGCCGNCCTATATGAGTCGATTACAAATCTGAC
  GNTACTAAACAGCTTGCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001885
• Insert Size: 980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001885.1, NP_001876.1
• RefSeq Size: 691 bp
• RefSeq ORF: 528 bp
• Locus ID: 1410
• Cytogenetics: 11q23.1
• Domains: HSP20, crystallin
• Gene Summary: 'Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2019]'