mu Crystallin (CRYM) (NM_001888) Human Untagged Clone

CAT#: SC118966

CRYM (untagged)-Human crystallin, mu (CRYM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRYM
• Synonyms: DFNA40; THBP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118966 sequence for NM_001888 edited (data generated by NextGen Sequencing)
  ATGAGCCGGGTACCAGCGTTCCTGAGCGCGGCCGAGGTGGAGGAACACCTCCGCAGCTCC
  AGCCTCCTCATCCCGCCTCTAGAGACGGCCCTGGCCAACTTCTCCAGCGGTCCCGAAGGA
  GGGGTCATGCAGCCCGTGCGCACCGTGGTGCCGGTGACCAAGCACAGGGGCTACCTGGGG
  GTCATGCCCGCCTACAGTGCTGCAGAGGATGCACTGACCACCAAGTTGGTCACCTTCTAC
  GAGGACCGCGGCATCACCTCGGTCGTCCCTTCCCACCAGGCTACTGTGCTACTCTTTGAG
  CCCAGCAATGGCACCCTGCTGGCGGTCATGGATGGAAATGTCATAACTGCAAAGAGAACA
  GCTGCAGTTTCTGCCATTGCCACCAAGTTTCTGAAACCTCCCAGCAGTGAAGTGCTGTGC
  ATCCTTGGGGCTGGGGTCCAGGCCTACAGCCATTATGAGATCTTCACAGAGCAGTTCTCC
  TTTAAGGAGGTGAGGATATGGAACCGCACCAAAGAAAATGCAGAGAAGTTTGCAGACACA
  GTGCAAGGAGAGGTACGGGTCTGTTCTTCGGTCCAGGAGGCTGTGGCAGGTGCAGATGTG
  ATCATCACAGTCACCCTGGCAACAGAGCCCATTTTGTTTGGTGAATGGGTGAAGCCAGGG
  GCTCACATCAATGCTGTTGGAGCCAGCAGACCTGACTGGAGAGAACTGGATGATGAGCTC
  ATGAAAGAAGCTGTGCTGTACGTGGATTCCCAGGAGGCTGCCCTGAAGGAGTCTGGAGAT
  GTCCTGCTGTCAGGGGCCGAGATCTTTGCTGAGCTGGGAGAAGTGATTAAGGGAGTGAAA
  CCAGCCCACTGTGAGAAGACCACCGTGTTCAAGTCTTTGGGAATGGCAGTGGAAGACACA
  GTTGCAGCCAAACTCATCTATGATTCCTGGTCATCTGGTAAATAA
  
  Clone variation with respect to NM_001888.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001888 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGAGATGAGCCGGGTAC
  CAGCGTTCCTGAGCGCGGCCGAGGTGGAGGAACACCTCCGCAGCTCCAGCCTCCTCATCC
  CGCCTCTAGAGACGGCCCTGGCCAACTTCTCCAGCGGTCCCGAAGGAGGGGTCATGCAGC
  CCGTGCGCACCGTGGTGCCGGTGACCAAGCACAGGGGCTACCTGGGGGTCATGCCCGCCT
  ACAGTGCTGCAGAGGATGCACTGACCACCAAGTTGGTCACCTTCTACGAGGACCGCGGCA
  TCACCTCGGTCGTCCCTTCCCACCAGGCTACTGTGCTACTCTTTGAGCCCAGCAATGGCA
  CCCTGCTGGCGGTCATGGATGGAAATGTCATAACTGCAAAGAGAACAGCTGCAGTTTCTG
  CCATTGCCACCAAGTTTCTGAAACCTCCCAGCAGTGAAGTGCTGTGCATCCTTGGGGCTG
  GGGTCCAGGCCTACAGCCATTATGAGATCTTCACAGAGCAGTTCTCCTTTAAGGAGGTGA
  GGATATGGAACCGCACCAAAGAAAATGCAGAGAAGTTTGCAGACACAGTGCAAGGAGAGG
  TACGGGTCTGTTCTTCGGTCCAGGAGGCTGTGGCAGGTGCAGATGTGATCATCACAGTCA
  CCCTGNCAACAGAGCCCATTTTGTTTGGTGAATGGGTGAAGCCAGGGGCTCACATCAATG
  CTGTTGGAGCCAGCAGACCTGACTGGAGAGAACTGGATGATGAGCTCATGAAAGAAGCTG
  TGCTGTACGTGGATTCCCAGNAGGCTGCCCTGAAAGAGTCTGGAGATGTCCTGCTGTCAG
  GNGCCGAGATCTTTGCTGAGCTGGGAGAAGTGATTAAGGGAGTGAAACCAGCCCACTGTG
  AGAAGACCACGTGNTNCAAGTCTTTGGAATGGCAGTGAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001888 unedited
  ATGTACCGCGGCACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTAACAATTGGA
  ATGTGCTTTATTTCAGGGAAAATATAAAGGGAAATGAATGCTATTATAACTTGGTAGAAC
  AGAAGAAATGGCTACCTAGCTTTGCTTTCCAACTACAAACATAAATGAGGATCTCAGCAT
  TTAAGGTAAAACATGATAAGCACAAAAGGAGAGTTCACTGGGGACTGGACTCCCTCATTT
  ACTCTAGAAATTATGAGAACCAGCAGCAATATTCCTCAAGCATCCATCTCAACATCAAGT
  TCCTTTGTTTTATTTACCAGATGACCAGGAATCATAGATGAGTTTGGCTGCAACTGTGTC
  TTCCACTGCCATTCCCAAAGACTTGAACACGGTGGTCTTCTCACAGTGGGCTGGTTTCAC
  TCCCTTAATCACTTCTCCCAGCTCAGCAAAGATCTCGGCCCCTGACAGCAGGACATCTCC
  AGACTCCTTCAGGGCAGCCTCCTGGGAATCCACGTACAGCACAGCTTCTTTCATGAGCTC
  ATCATCCAGTTCTCTCCAGTCAGGTCTGCTGGCTCCAACAGCATTGATGTGAGCCCCTGG
  CTTCACCCATTCACCAAACAAAATGGGCTCTGTTGCCAGGGTGACTGTGATGATCACATC
  TGCACCTGCCACAGCCTCCTGGACCGAAGACAGACCCGTACCTCTCCTTGCACTGTGTCT
  GCAAACTTCTCTGCATTTTCTTTGGTGCCGGTTCATATCCTCACCTCCTTAAGGAAAACT
  GCTCTGGAAAACCTCATATGGCTGGGAGCCCGGACCCCACCCCAAGATGACAGCACTTCA
  CTGCTGGGAGGTTCAAACCTGGGGGCATGGCAAAAACTGCACTGTCCTCTTGCATTAATA
  CATTTCTCCTTGACGCCACAGGGGGCCTGGCTGGGCTCAAAGTACCCATACCCGGGGGAA
  GGCAACCCGGTGATCCGGGTCCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001888
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001888.2, NP_001879.1
• RefSeq Size: 1303 bp
• RefSeq ORF: 945 bp
• Locus ID: 1428
• Cytogenetics: 16p12.2
• Domains: ODC_Mu_crystall
• Gene Summary: 'Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]'