CCN2 (NM_001901) Human Untagged Clone

CAT#: SC118973

CTGF (untagged)-Human connective tissue growth factor (CTGF)


  "NM_001901" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CCN2
Synonyms CTGF; HCS24; IGFBP8; NOV2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118973 sequence for NM_001901 edited (data generated by NextGen Sequencing)
ATGACCGCCGCCAGTATGGGCCCCGTCCGCGTCGCCTTCGTGGTCCTCCTCGCCCTCTGC
AGCCGGCCGGCCGTCGGCCAGAACTGCAGCGGGCCGTGCCGGTGCCCGGACGAGCCGGCG
CCGCGCTGCCCGGCGGGCGTGAGCCTCGTGCTGGACGGCTGCGGCTGCTGCCGCGTCTGC
GCCAAGCAGCTGGGCGAGCTGTGCACCGAGCGCGACCCCTGCGACCCGCACAAGGGCCTC
TTCTGTGACTTCGGCTCCCCGGCCAACCGCAAGATCGGCGTGTGCACCGCCAAAGATGGT
GCTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCGGAGAGTCCTTCCAGAGCAGCTGC
AAGTACCAGTGCACGTGCCTGGACGGGGCGGTGGGCTGCATGCCCCTGTGCAGCATGGAC
GTTCGTCTGCCCAGCCCTGACTGCCCCTTCCCGAGGAGGGTCAAGCTGCCCGGGAAATGC
TGCGAGGAGTGGGTGTGTGACGAGCCCAAGGACCAAACCGTGGTTGGGCCTGCCCTCGCG
GCTTACCGACTGGAAGACACGTTTGGCCCAGACCCAACTATGATTAGAGCCAACTGCCTG
GTCCAGACCACAGAGTGGAGCGCCTGTTCCAAGACCTGTGGGATGGGCATCTCCACCCGG
GTTACCAATGACAACGCCTCCTGCAGGCTAGAGAAGCAGAGCCGCCTGTGCATGGTCAGG
CCTTGCGAAGCTGACCTGGAAGAGAACATTAAGAAGGGCAAAAAGTGCATCCGTACTCCC
AAAATCTCCAAGCCTATCAAGTTTGAGCTTTCTGGCTGCACCAGCATGAAGACATACCGA
GCTAAATTCTGTGGAGTATGTACCGACGGCCGATGCTGCACCCCCCACAGAACCACCACC
CTGCCGGTGGAGTTCAAGTGCCCTGACGGCGAGGTCATGAAGAAGAACATGATGTTCATC
AAGACCTGTGCCTGCCATTACAACTGTCCCGGAGACAATGACATCTTTGAATCGCTGTAC
TACAGGAAGATGTACGGAGACATGGCATGA

Clone variation with respect to NM_001901.2
>OriGene 5' read for NM_001901 unedited
GGGGGGGAGNNNNGTTNCTTCTCNNNNNNNGGGTCGAGAATTGTNATACCACTTATATAG
GCGGCCGCGNAATTCGCACCGAGGNAAAGNAGACAGCCAGTGCGACTCCACCCTCCAGCT
CGACGGCAGCCGCCCCGGCCGACAGCCCCGAGACGACAGCCCGGCGCGTCCCGGTCCCCA
CCTCCGACCACCGCCAGCGCTCCAGGCCCCGCCGCTCCCCGCTCGCCGCCACCGCGCCCT
CCGCTCCGCCCGCAGTGCCAACCATGACCGCCGCCAGTATGGGCCCCGTCCGCGTCGCCT
TCGTGGTCCTCCTCGCCCTCTGCAGCCGGCCGGCCGTCGGCCAGAACTGCAGCGGGCCGT
GCCGGTGCCCGGACGAGCCGGCGCCGCGCTGCCCGGCGGGCGTGAGCCTCGTGCTGGACG
GCTGCGGCTGCTGCCGCGTCTGCGCCAAGCAGCTGGGCGAGCTGTGCACCGAGCGCGACC
CCTGCGACCCGCACAAGGGCCTCTTCTGTGACTTCGGCTCCCCGGCCAACCGCAAGATCG
GCGTGTGCACCGCCAAAGATGGTGCTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCG
GAGAGTCCTTCCAGAGCAGCTGCAAGTACCAGTGCACGTGCCTGGACGGNGCGGTGGGCT
GCATGCCCCTGTGCAGCATGGACGTTCGTCTGCCCAGCCCTGACTGCCCCTTCCCGAGGA
GGGNTCAGCTGCCCNGGAAATGCTGCNNAGAGTGGGTGTGTGACAAGCCCAGGACCAAAC
CGTGGNNTGGCCTGCCCCTCGCGCTTACCGACTGGNAGACACGTTTGGNCCCANACCCCA
CTATGATTANAGCCAACTGCCTGGTCCAGACACAGAGTGGNAGCGCCTGTTTCAAGACTG
TTGGATGGGCATCTTCCACCCGGGTTACCAATGACAACGCTCCTGCAGGCTANAGAAGCA
NAGCCGCCTGTGCATGGTCAGGCCTTGCGAANCTGACCTGGAAGAGAACATTTAGAGGCC
AAAAA
>OriGene 3' read for NM_001901 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTAAAAAAAAAAATACCCTTTTTTTTCAACTAAAAAGGGGCAAACATGTAACT
TTTGGTCACACTTTCAACAAATAAACTGTCCGAAAAACAGTCTTTTCAAAGTAAAAACAA
ATGCTTCCAGGGGAAAAAACTGATCAGCTTTTTAAAGTCACTCAGTTTTTAATAAAGGCC
ATTTGTTCATTAAAAGGGAGGCTACCACATTTCCTACCTAAAAATCACCCTGCCAAGGAC
ACTGATGCCTCCCCTTTGCAAACAATTTGTTTTGACGGACTGTCTTTTTATTTGAGCAAA
ATTTCCATATAAGTATCCATTTCATGCTTTGAACGATCAAACAAGCTTTACATTCTACCT
ATGGGGTTCAAAAATTGAGGCTAACATTGAAATATCAAAGCCTTAAAAACAAAAATAAAA
AGGCCCAAACAACTTTAAATTAACTTAAATAACTGCCCCTATATTTTTTTTTCCCCACAC
CCCCACCCCCCCAATTTTTACAATATAAATTTTAAAAAATCTGGCTTTGTACAGGCAAAT
TCACTTGCCACAAGCTGTCCAGTTTATTCGACGGATTTCGATTCCTGACAGGGTCTTTCG
AACAAAATGTCAGAACTGAATCTGTTGTTTTGACTTAGAGAACACTTGACTAATCCCTTG
ATGGCTGGAAATGCCACTCTAACTGTACTGGGGCTACAGCCGGTAAGGAACCCCCTAATT
TTCTTTTAATTACCCTTAAAAGCCGCTTTTACCCGATAAAAGCTTTCGGAGAACTACCTT
TTTGGGGGCCCCCCCCCTGTTTTAGGCCCCCCTTATATACTTTGGGGCTGGGACAAGGGA
ATCCCTGGAAGGTTTAACTTTTCCAA
Restriction Sites NotI-NotI     
ACCN NM_001901
Insert Size 2500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001901.1, NP_001892.1
RefSeq Size 2312 bp
RefSeq ORF 1050 bp
Locus ID 1490
Cytogenetics 6q23.2
Domains IB, tsp_1, VWC, CT, Cys_knot
Protein Families Druggable Genome, Secreted Protein
Gene Summary 'The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]'

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