CLCN4 (NM_001830) Human Untagged Clone

CAT#: SC118976

CLCN4 (untagged)-Human chloride channel 4 (CLCN4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLCN4
• Synonyms: ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118976 sequence for NM_001830 edited (data generated by NextGen Sequencing)
  ATGGTCAATGCGGGAGCGATGAGTGGCTCTGGAAACCTGATGGATTTCCTCGATGAGCCG
  TTCCCTGATGTGGGGACGTATGAGGACTTCCACACCATCGACTGGCTAAGGGAAAAGTCA
  CGGGACACCGACAGACACAGGAAGATCACCAGCAAGAGCAAGGAGTCCATATGGGAGTTC
  ATCAAGAGCCTGCTGGATGCCTGGTCGGGATGGGTGGTGATGCTGCTCATCGGCCTGCTG
  GCGGGCACCTTGGCTGGGGTCATCGATCTCGCCGTGGACTGGATGACGGACCTGAAGGAG
  GGGGTCTGCCTGTCTGCCTTCTGGTATAGCCATGAGCAGTGTTGCTGGACTTCTAACGAG
  ACCACTTTTGAGGACAGAGACAAGTGTCCCCTGTGGCAGAAATGGTCGGAGCTGCTGGTG
  AATCAGTCAGAGGGTGCCAGTGCTTACATTCTGAATTACTTAATGTACATCCTATGGGCG
  CTGCTGTTTGCATTTTTGGCTGTCTCCCTGGTGCGTGTATTTGCACCATATGCCTGTGGC
  TCTGGCATACCAGAGATAAAGACCATTTTGAGCGGCTTTATCATCAGGGGCTACTTGGGG
  AAGTGGACCCTGCTAATCAAGACAGTCACGCTGGTGCTGGTAGTGTCCTCCGGTCTGAGC
  CTTGGGAAGGAAGGGCCGCTAGTGCACGTGGCTTGTTGCTGTGGCAACTTCTTCAGCAGC
  CTTTTCTCCAAGTACAGCAAGAATGAGGGCAAGAGGCGGGAGGTGCTTTCAGCTGCAGCG
  GCTGCTGGAGTCTCTGTTGCCTTTGGTGCACCAATTGGAGGCGTGCTTTTCAGTCTAGAA
  GAGGTCAGTTACTACTTTCCCCTGAAGACCTTGTGGAGGTCATTTTTCGCAGCCCTGGTG
  GCGGCCTTTACGCTGAGATCCATCAATCCCTTTGGGAATAGCCGTCTCGTTCTCTTTTAT
  GTGGAATACCACACGCCCTGGTACATGGCTGAACTCTTCCCCTTCATCCTGCTTGGGGTC
  TTCGGGGGCTTGTGGGGAACCCTCTTCATCCGCTGCAACATCGCCTGGTGCAGGAGGCGC
  AAGACCACCAGGCTGGGGAAGTACCCGGTGCTGGAGGTCATTGTGGTGACTGCCATCACT
  GCCATCATTGCCTACCCCAATCCCTACACACGCCAGAGCACCAGCGAGCTCATTTCTGAG
  CTGTTCAATGACTGTGGAGCCCTTGAGTCTTCCCAGCTCTGTGACTACATCAATGACCCC
  AACATGACTCGGCCTGTGGATGACATTCCAGACCGGCCGGCTGGTGTCGGTGTTTACACG
  GCCATGTGGCAGCTGGCCCTGGCACTGATCTTCAAAATCGTCGTTACCATATTTACCTTT
  GGCATGAAGATCCCGTCGGGCCTCTTCATCCCCAGCATGGCTGTGGGCGCGATAGCGGGC
  AGGATGGTGGGAATTGGCGTGGAGCAGCTGGCCTACCATCACCATGACTGGATCATCTTC
  AGGAACTGGTGCAGACCCGGTGCAGACTGTGTCACGCCAGGGCTGTACGCAATGGTGGGA
  GCTGCGGCCTGCCTCGGTGGAGTTACCAGGATGACGGTGTCATTGGTGGTCATCATGTTT
  GAATTAACCGGGGGTCTGGAGTACATCGTGCCCCTGATGGCGGCGGCTGTGACCAGCAAG
  TGGGTAGCTGATGCATTTGGGAAAGAAGGCATCTACGAGGCCCACATCCACTTAAATGGG
  TACCCTTTCCTTGACGTGAAGGACGAGTTTACTCACCGCACACTGGCCACCGACGTCATG
  CGGCCCCGGCGGGGAGAGCCGCCACTGTCGGTGCTCACCCAGGACAGCATGACTGTCGAG
  GACGTGGAGACGCTCATCAAGGAGACCGACTACAACGGCTTCCCCGTGGTGGTCTCCAGA
  GACTCCGAGCGCCTCATTGGATTTGCCCAGAGGAGGGAACTGATTCTCGCAATAAAGAAC
  GCCAGACAGAGGCAGGAGGGCATTGTGAGCAATTCCATCATGTACTTCACGGAGGAACCC
  CCCGAGCTGCCGGCCAACAGCCCACATCCCCTGAAGCTGCGGCGCATCCTGAACCTCAGC
  CCGTTTACAGTGACAGACCACACTCCGATGGAAACGGTGGTGGATATCTTCCGGAAACTG
  GGGCTTCGGCAGTGCCTGGTGACGCGGAGCGGGAGACTTCTTGGCATCATCACAAAAAAG
  GATGTTCTGAGACATATGGCCCAGATGGCAAACCAGGACCCCGAATCCATCATGTTTAAT
  TAG
  
  Clone variation with respect to NM_001830.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001830 unedited
  TATAACCCCGCCCGTTGNAGCANTGGGCGGGTAGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTTCATTTAGGTGGACACTATAGAAATACAAGCTAACTTGTTCTTTTTGCAGCGG
  CCGCGAATTCGGCACGAGGGCCGCGGGCGCGAATGCGGCACTGCAGGTGTAATTAGCATG
  GTCAATGCGGGAGCGATGAGTGGCTCTGGAAACCTGATGGATTTCCTCGATGAGCCGTTC
  CCTGATGTGGGGACGTATGAGGACTTCCACACCATCGACTGGCTAAGGGAAAAGTCACGG
  GACACCGACAGACACAGGAAGATCACCAGCAAGAGCAAGGAGTCCATATGGGAGTTCATC
  AAGAGCCTGCTGGATGCCTGGTCGGGATGGGTGGTGATGCTGCTCATCGGCCTGCTGGCG
  GGCACCTTGGCTGGGGTCATCGATCTCGCCGTGGACTGGATGACGGACCTGAAGGAGGGG
  GTCTGCCTGTCTGCCTTCTGGTATAGCCATGAGCAGTGTTGCTGGACTTCTAACGAGACC
  ACTTTTGAGGACAGAGACAAGTGTCCCCTGTGGCAGAAATGGTCGGAGCTGCTGGTGAAT
  CAGTCAGAGGGTGCCAGTGCTTACATTCTGAATTACTTAATGTACATCCTATGGGCGCTG
  CTGTTTGCATTTTTGGCTGTCTCCCTGGTGCGTGTATTTGCACCATATGCCTGTGGCTCT
  GGCATACCAGAGATAAAGACCATTTTGAGCGGCTTTATCATCAGGGGCTACTTGGGGAAG
  TGGACCCCTGCTATCAAGACAGTCACGCTGGTGCTGGTAGTGTCCTCCCGTCTGAGCCTT
  GGAAGGAAGGCCCCTAGTGCACGTGGCTTGNTGCTGTGGCACTTCTTCAGCAGCCTTTTC
  TCCAGTACAGCAGAATGAGGGGCAGAGCCGGGAGTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001830 unedited
  GTGGGCCTACTATGTACCGTGGCCGCTTTCTATGATCGGTTTTTTTTTTTTTTTTTTGTT
  GCAGACTCTGTGCTTGTTTTATTTTTAAAAAAGAGTTCAGACATTTAATATTTAATAAGG
  TGTCAACGTTTACACAGAGTGTTACAATAATAAAAAAACCAACACGTTGCTATGTGGTCG
  TTCCAACACCAAATGTGAATATATCTCATCAAAGTCGCACGTAGGCTCATTCCCAGCACC
  TCTAGGATAGCAAATAAATAGTTATTACCGCGCCAGTGAAAATAGGGTCTGCTTAATGTT
  TCCCAAGTCAGATATACAGGCTCTCTCCTCATGTAAATCATTTCAAAGGCTAGAAGCTTA
  GTCTGATAGAACACTGCCCATACAAAATTAAAGCATAATATTACAATTATGTGGTAATTC
  AATAGTTCTCCTTGGGTTTCTCATCTTTTCCAGCTTAGCATCTAATAATGAATACTAAGT
  AGAATTTGCACATGTTGGTAATTTAAAGTAATCAGTTCTCACCTAAGTGAAACAAACTGC
  TCTAAAATTTTCCATTTCTGTGACTTATTAGGCTCAAAATCTATCAGTTTACACATCTCC
  TTTGTAAGGTCTTTGGGAAGGACTGAATAAAGAACTGAGATTACCTGGTTCAAAGTAACA
  GTAAGCACTGAATATTGAATGACTTCGACTTCTAGCACATGCTGCCTTTCCCTGAAAGCC
  ACATGAGCATCATGCAATCGCATGGGATGCACTATAAACGACTTAGAAGAAAACACATGT
  CACTTCCAGGTGCCCTTTATTGGTGGCTTTTGACAACACATTTTCTCAGTGAGAGCAGAA
  AAATGTGATGATATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001830
• Insert Size: 7000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001830.2, NP_001821.1
• RefSeq Size: 4562 bp
• RefSeq ORF: 2283 bp
• Locus ID: 1183
• Cytogenetics: Xp22.2
• Domains: CBS, voltage_CLC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: 'The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.