Acidic Calponin (CNN3) (NM_001839) Human Untagged Clone

CAT#: SC118982

CNN3 (untagged)-Human calponin 3, acidic (CNN3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CNN3
• Synonyms: calponin, acidic; calponin 3; calponin 3, acidic; dJ639P13.2.2 (acidic calponin 3)
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118982 sequence for NM_001839 edited (data generated by NextGen Sequencing)
  ATGACCCACTTCAACAAGGGCCCTTCCTATGGGCTCTCGGCCGAAGTCAAGAACAAGATT
  GCTTCCAAGTATGATCATCAGGCAGAAGAAGATCTTCGCAATTGGATAGAAGAGGTGACA
  GGCATGAGCATTGGCCCCAACTTCCAGCTGGGCTTAAAGGATGGCATCATCCTCTGCGAA
  CTTATAAACAAGCTACAGCCAGGCTCAGTGAAGAAGGTCAACGAGTCCTCACTGAACTGG
  CCTCAGTTGGAGAATATTGGCAACTTTATTAAAGCTATTCAGGCTTATGGTATGAAGCCA
  CATGACATATTCGAAGCAAATGATCTTTTTGAGAATGGAAACATGACCCAGGTTCAGACT
  ACTCTGGTGGCTCTAGCAGGTCTGGCTAAAACAAAAGGATTCCATACAACCATTGACATT
  GGAGTTAAGTATGCAGAAAAACAAACAAGACGTTTTGATGAAGGAAAATTAAAAGCTGGC
  CAAAGTGTAATTGGTCTGCAGATGGGAACCAACAAATGTGCCAGCCAGGCAGGTATGACA
  GCTTACGGGACTAGGAGGCATCTTTATGATCCCAAAATGCAAACTGACAAACCTTTTGAC
  CAGACCACAATTAGTCTGCAGATGGGCACTAATAAAGGAGCCAGCCAGGCAGGGATGTTA
  GCACCAGGTACCAGAAGAGACATCTATGATCAGAAGCTAACATTACAGCCGGTGGACAAC
  TCGACAATTTCCCTACAGATGGGTACCAACAAAGTTGCTTCCCAGAAAGGAATGAGTGTG
  TATGGGCTTGGGCGGCAAGTATATGATCCCAAATACTGTGCTGCTCCTACAGAACCTGTC
  ATTCACAACGGAAGCCAAGGAACAGGAACAAATGGTTCGGAAATCAGTGATAGTGATTAT
  CAGGCAGAATACCCTGATGAGTATCATGGCGAGTACCAGGATGACTACCCCAGAGATTAC
  CAATATAGCGACCAAGGCATTGATTATTAG
  
  Clone variation with respect to NM_001839.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001839 unedited
  CGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCAGCGGAGCTCGAGGCTCTGTAGCA
  CCCAGTTGGACCCAGTCGCCCAGGCACCGCCCGCGCCCCGCGGCCGTCTCCTGCGCCTCC
  CAGAGGAGGGAGTTCGAGCATCCCCCGCGGCGGCCACTCCTCGGGGCCAGGAGCGGGGAA
  GCGAAGTGCGAGAGACCCCGGACCCCAGCGCTGTCTCTTCCCGCCGCCCGAACCACCATG
  ACCCACTTCAACAAGGGCCCTTCCTATGGGCTCTCGGCCGAAGTCAAGAACAAGATTGCT
  TCCAAGTATGATCATCAGGCAGAAGAAGATCTTCGCAATTGGATAGAAGAGGTGACAGGC
  ATGAGCATTGGCCCCAACTTCCAGCTGGGCTTAAAGGATGGCATCATCCTCTGCGAACTT
  ATAAACAAGCTACAGCCAGGCTCAGTGAAGAAGGTCAACGAGTCCTCACTGAACTGGCCT
  CAGTTGGAGAATATTGGCAACTTTATTAAAGCTATTCAGGCTTATGGTATGAAGCCACAT
  GACATATTCGAAGCAAATGATCTTTTTGAGAATGGAAACATGACCCAGGTTCAGACTACT
  CTGGTGGCTCTAGCAGGTCTGGCTAAAACAAAAGGATTCCTACAACCATTGACATTGGAG
  TTAAGTATGCAGAANAACAAACAAGACGTTNTGATGAAGGAAAATTAAAAGCTGGCCCAA
  AGTGTAATTGGTCTGCAGATGGGAACCAACANATGTGCCAGCCANNGCAGTATGACAGCT
  TACGGGACTAGGAGGCATCTTTATGATCCCANAATGCAACTGACAACCTTTTGACCAGAC
  ACCATTAGTCTGCAGATGGGCACTANTAAAGGAGCCCAGCAGCAGGGATGGNTAGCACCC
  AGTACCAGAGAGACATTCATGATCAGAAGCTACATTACAGCCGGTGGACACTCGACATTT
  CCTACGAATGGTCAAACAAGATGCTTCAAAGGGATGAATGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001839 unedited
  CCCCCCCCCCCTCCCACCCACCATCCCTTACTTGCCCGCGGCCGCATTCTANGATCGGTT
  TTTTTTTTTTTTTTTTCCACTGATCATGACTTTATTTAAAAATTAGCAAACAATACTGTA
  GAAACATTGATATGTAAATTTCTAAAATGCTGCATCTTAAATTTAGTTGGCAAAGACCAC
  ATTTAGCAATAAGCATGAGTTTAGTCTTCCATGTAGAAACCAGATACACTAAACTGCAAA
  AAAAAAAAAAAAAAAGTTTCCTATTGTTTGAAAATACCAGTTTAATATAAGATTGCAAAA
  ATGCATAGATTTTTGCATAAAAGAACTGGCTGTACAAGAGTACTCCCCTTTCACAGTATT
  CCTTTTTACTTCATATGCGAGTTATTGATTATGCTGCAGGATTTAACTATTACAGCACTA
  AAAGGCAACTATTGAGGGAAGAGGCAGAAAAAGGAAACAGGAATGTACGTAAGGCAATTT
  TTCTTAAAAGTACAATAAGCTCAATAGTGTTTTAGGAACACAAGATAAAAATTACTCACG
  GCTAGCTTGGTTCTCACTGAATAAAAACAAAGGACTAAATACTGAGCTCCTTCTGTGTGG
  ATCTAATAATCCATGCCCTTGCTCCGCTATATTGGTAACTCTCTGGCGCAGTCATCCTGG
  CACTCCCCATGATACTCACCAGGTATTCTGCCTGAAATCACTTCACTGATTTCCGACCAC
  TTGTTCTGTTCCTTGGCTTCCGTTGGAAAGACAGGTTCTGTAGGAGCCACACAGTTTTGG
  GAATATTTACTTGGCCGCCAAGCCCATTCCCCTCCATTCTTTTTGGGAAACACATTTGTT
  GTTACCCCTTCTGTAGGAAAATCGTCCAATTTCCCCCCCGGCTGGAAAGGTAACCTTCCG
  ACAATAAAAGCTCCTTTCTGGGCCCTGGGCGCTAACACCCTCGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001839
• Insert Size: 1790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001839.2, NP_001830.1
• RefSeq Size: 2045 bp
• RefSeq ORF: 990 bp
• Locus ID: 1266
• Cytogenetics: 1p21.3
• Domains: calponin, CH
• Gene Summary: 'This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.