Cannabinoid Receptor II (CNR2) (NM_001841) Human Untagged Clone

CAT#: SC118984

CNR2 (untagged)-Human cannabinoid receptor 2 (macrophage) (CNR2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CNR2
• Synonyms: CB-2; CB2; CX5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118984 sequence for NM_001841 edited (data generated by NextGen Sequencing)
  ATGGAGGAATGCTGGGTGACAGAGATAGCCAATGGCTCCAAGGATGGCTTGGATTCCAAC
  CCTATGAAGGATTACATGATCCTGAGTGGTCCCCAGAAGACAGCTGTTGCTGTGTTGTGC
  ACTCTTCTGGGCCTGCTAAGTGCCCTGGAGAACGTGGCTGTGCTCTATCTGATCCTGTCC
  TCCCACCGGCTCCGCCGGAAGCCCTCATACCTGTTCATTGGCAGCTTGGCTGGGGCTGAC
  TTCCTGGCCAGTGTGGTCTTTGCATGCAGCTTTGTGAATTTCCATGTTTTCCATGGTGTG
  GATTCCAAGGCTGTCTTCCTGCTGAAGATTGGCAGCGTGACTATGACCTTCACAGCCTCT
  GTGGGTAGCCTCCTGCTGACCGCCATTGACCGATACCTCTGCCTGCGCTATCCACCTTCC
  TACAAAGCTCTGCTCACCCGTGGAAGGGCACTGGTGACCCTGGGTATCATGTGGGTCCTC
  TCAGCACTAGTCTCCTACCTGCCCCTCATGGGATGGACTTGCTGTCCCAGGCCCTGCTCT
  GAGCTTTTCCCACTGATCCCCAATGACTACCTGCTGAGCTGGCTCCTGTTCATCGCCTTC
  CTCTTTTCCGGAATCATCTACACCTATGGGCATGTTCTCTGGAAGGCCCATCAGCATGTA
  GCCAGCTTGTCTGGCCACCAGGACAGGCAGGTGCCAGGAATGGCCCGAATGAGGCTGGAT
  GTGAGGTTGGCCAAGACCCTAGGGCTAGTGCTGGCTGTGCTCCTCATCTGTTGGTTCCCA
  GTGCTGGCCCTCATGGCCCACAGCCTGGCCACTACGCTCAGTGACCAGGTCAAGAAGGCC
  TTTGCCTTCTGCTCCATGCTGTGCCTCATCAACTCCATGGTCAACCCTGTCATCTATGCT
  CTACGGAGTGGAGAGATCCGCTCCTCTGCCCATCACTGCCTGGCTCACTGGAAGAAGTGT
  GTGAGGGGCCTTGGGTCAGAGGCAAAAGAAGAAGCCCCAAGATCCTCAGTCACGGAGACA
  GAGGCTGATGGGAAAATCACTCCGTGGCCAGATTCCAGAGATCTAGACCTCTCTGATTGC
  TGA
  
  Clone variation with respect to NM_001841.2
  188 a=>g;189 a=>g;465 c=>t;660 g=>a;751 t=>c;846 t=>c;999 g=>a;1014 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001841 unedited
  GGGTTGGGGGGNGNNNNNGGGTTNNNNNNCCGGTTCAAAATTGTATACGACTCATATAGG
  CGGCCGCGAATTCACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATA
  GCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATTCGGCACCAG
  GCTGGCTTGGGGTGGCACTCAACAGGTGCTCTGAGTGGCACCCACGGCCAGGTCCTGGGA
  GAGGACAGAAAACAACTGGGACTCCTCAGCCCCCGGCAGCTCCCAGTGCCCAGCCACCCA
  CAACACAACCCAAAGCCTTCTAGACAAGCTCAGTGGAATCTGAAGGGCCCACCCCATGGA
  GGAATGCTGGGTGACAGAGATAGCCAATGGCTCCAAGGATGGCTTGGATTCCAACCCTAT
  GAAGGATTACATGATCCTGAGTGGTCCCCAGAAGACAGCTGTTGCTGTGTTGTGCACTCT
  TCTGGGCCTGCTAAGTGCCCTGGAGAACGTGGCTGTGCTCTATCTGATCCTGTCCTCCCA
  CCGGCTCCGCCGGAAGCCCTCATACCTGTTCATTGGCAGCTTGGCTGGGGCTGACTTCCT
  GGCCAGTGTGGTCTTTGCATGCAGCTTTGTGAATTTCCATGTTTTCCATGGTGTGGATTC
  CAAGGCTGTCTTCCTGCTGAAGATTGGCAGCGTGACTATGACCTTCACAGCCTCTGTGGG
  TAGCCTCCTGCTGACCGCCATTGACCGATACCTCTGCCTGCGCTATCCACCTTCCTACAN
  AGCTCTGCTCACCCGTGGAAGGGCACTGGTGACCCTGNGTATCATGTGGGTCCTCTCAGC
  ACTAGTCTNCTACCTGCCCCTCATGGGATGGACTTGCTGTCCCAGCCCTGCTCTGAGCTT
  TTCCACTGATCCCCATGACTACCTGCTGAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001841 unedited
  GGGGGTAGGTTCATCCCGAATATTTACAAAAGGGCCCTCTGTTTNCTATTTTTNCCTTTG
  NGAGTCCCAATAATTGCATTAGNATTCCTCTGCAATCTTCCTGCCACCAACAATTTCTCT
  TGTTGGCTGTTTTAGGCTCCTCTGGGAGGAACCCAGCCTCCCTCATATGAAAAATGAGTA
  CCCTGGGATCTATTTTATTTTATTTTTTTTGAGACAGAGTCTTGCTCTGTCACCCAGGCT
  GGAGTGCAGTGGTGCAATCTCGGCTCACTGCAACCTCTGCCTCCAGGGTTCAAGCAATTC
  TCCTGTCTCAGCCTCCCGAGTAGCTGGGACTACAGATGCCTGCCACCATGCCCTGCCAAT
  TTTTGTATTTTTAGTAGAGACAGGGTTTCAACCATATTGGTCAGGCTGGTCTCAAACTCC
  CGACCCCAGGTGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCC
  ACCATGCCTGGCCCCTGAGATCTTTAAAATCATGGAGCTGTATTCTAGCTGGGGTCAATA
  GACTGAAATGTCAGCCCCATACCTGGCATATAGGAAGGACATTTCCCCTCCTCCCACTTG
  CTCTGCTTATTATATGTAATAGGGGCGTGGAGGAGGCAAGGGAGCTGAATTGGGGGCAGA
  TAGGGGAACAGTTTGGAGATTTATACAATCTTTGTGAATATCTTCCTTTTTTTCTTCGAG
  TTCGTTAGACTAGGACATTGCCTGCTTCCAGCAGCACCACCAGGACTGCTGCTTGGAAGA
  CTTCACAGGGTCAAGAAAATTCTCCAAAGATTTTTGTGTCTCACGCGATCCCCACCTCCT
  CTGTTATTTCCAGGGACCTGAGGTTACAGGGGGGGATTATCAACATATCACAAAAGGGTC
  CTTCGCCAACCACAAGATGCCCCAAACTGAAGGTTGCTCTTTTATCTTGAAGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001841
• Insert Size: 3100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001841.1, NP_001832.1
• RefSeq Size: 1776 bp
• RefSeq ORF: 1083 bp
• Locus ID: 1269
• Cytogenetics: 1p36.11
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]'