R Cadherin (CDH4) (NM_001794) Human Untagged Clone

CAT#: SC119019

CDH4 (untagged)-Human cadherin 4, type 1, R-cadherin (retinal) (CDH4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDH4
• Synonyms: CAD4; R-CAD; RCAD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001794 edited
  AGCGGCGGCGGTGGTCTCGGCGGCGGCGGCGGCGGCGGCGGCAGGGAGCGGGCTCCCGGT
  GCCGGGCACCGGGCGGGCGGCGGGGAAGATGACCGCGGGCGCCGGCGTGCTCCTTCTGCT
  GCTCTCGCTCTCCGGCGCGCTCCGGGCCCATAATGAGGATCTTACAACTAGAGAGACCTG
  CAAGGCTGGGTTCTCTGAAGATGATTACACGGCATTAATCTCCCAAAATATTCTAGAAGG
  GGAAAAGCTACTTCAAGTCAAGTTCAGCAGCTGTGTGGGGACCAAGGGGACACAATATGA
  GACCAACAGCATGGACTTCAAAGTTGGGGCAGATGGGACAGTCTTCGCCACCCGGGAGCT
  GCAGGTCCCCTCCGAGCAGGTGGCGTTCACGGTGACTGCATGGGACAGCCAGACAGCAGA
  GAAATGGGACGCCGTGGTGCGGTTGCTGGTGGCCCAGACCTCGTCCCCGCACTCTGGACA
  CAAGCCGCAGAAAGGAAAGAAGGTCGTGGCTCTGGACCCCTCTCCGCCTCCGAAGGACAC
  CCTGCTGCCGTGGCCCCAGCACCAGAACGCCAACGGGCTGAGGCGGCGCAAACGGGACTG
  GGTCATCCCGCCCATCAACGTGCCCGAGAACTCGCGCGGGCCCTTCCCGCAGCAGCTCGT
  GAGGATCCGGTCCGACAAAGACAATGACATCCCCATCCGGTACAGCATCACGGGAGTGGG
  CGCCGACCAGCCCCCCATGGAGGTCTTCAGCATTGACTCCATGTCCGGCCGGATGTACGT
  CACAAGGCCCATGGACCGGGAGGAGCACGCCTCTTACCACCTCCGAGCCCACGCTGTGGA
  CATGAATGGCAACAAGGTGGAGAACCCCATCGACCTGTACATCTACGTCATCGACATGAA
  TGACAACCGCCCTGAGTTCATCAACCAGGTCTACAACGGCTCCGTGGACGAGGGCTCCAA
  GCCAGGCACCTACGTGATGACCGTCACGGCCAACGATGCTGACGACAGCACCACGGCCAA
  CGGGATGGTGCGGTACCGGATCGTGACCCAGACCCCACAGAGCCCGTCCCAGAATATGTT
  CACCATCAACAGCGAGACTGGAGATATCGTCACAGTGGCGGCTGGCCTGGACCGAGAGAA
  AGTTCAGCAGTACACAGTCATCGTTCAGGCCACAGATATGGAAGGAAATCTCAACTATGG
  CCTCTCAAACACAGCCACAGCCATCATCACGGTGACAGATGTGAATGACAACCCGCCAGA
  ATTTACCGCCAGCACGTTTGCAGGGGAGGTCCCCGAAAACCGCGTGGAGACCGTGGTCGC
  AAACCTCACGGTGATGGACCGAGATCAGCCCCACTCTCCAAACTGGAATGCCGTTTACCG
  CATCATCAGTGGGGATCCATCCGGGCACTTCAGCGTCCGCACAGATCCCGTAACCAACGA
  GGGCATGGTCACCGTGGTGAAGGCAGTCGACTACGAGCTCAACAGAGCTTTCATGCTGAC
  AGTGATGGTGTCCAACCAGGCGCCCCTGGCCAGCGGAATCCAGATGTCCTTCCAGTCCAC
  GGCAGGGGTGACCATCTCCATCATGGACATCAACGAGGCTCCCTACTTCCCCTCAAACCA
  CAAGCTGATCCGCCTGGAGGAGGGCGTGCCCCCCGGCACCGTGCTGACCACGTTTTCAGC
  TGTGGACCCTGACCGGTTCATGCAGCAGGCTGTGAGATACTCAAAGCTGTCAGACCCAGC
  GAGCTGGCTGCACATCAATGCCACCAACGGCCAGATCACCACGGCGGCAGTGCTGGACCG
  TGAGTCCCTCTACACCAAAAACAACGTCTACGAGGCCACCTTCCTGGCAGCTGACAATGG
  GATACCCCCGGCCAGCGGCACCGGGACCCTCCAGATCTATCTCATTGACATCAACGACAA
  CGCCCCTGAGCTGCTGCCCAAGGAGGCGCAGATCTGCGAGAAGCCCAACCTGAACGCCAT
  CAACATCACGGCGGCCGACGCTGACGTCGACCCCAACATCGGCCCCTACGTCTTCGAGCT
  GCCCTTTGTCCCGGCGGCCGTGCGGAAGAACTGGACCATCACCCGCCTGAACGGTGACTA
  TGCCCAACTCAGCTTGCGCATCCTGTACCTGGAGGCCGGGATGTATGACGTCCCCATCAT
  CGTCACAGACTCTGGAAACCCTCCCCTGTCCAACACGTCCATCATCAAAGTCAAGGTGTG
  CCCATGTGATGACAACGGGGACTGCACCACCATTGGCGCAGTGGCAGCGGCTGGTCTGGG
  CACCGGTGCCATCGTGGCCATCCTCATCTGCATCCTCATCCTGCTGACCATGGTCCTGCT
  GTTTGTCATGTGGATGAAGCGGCGAGAGAAGGAGCGCCACACGAAGCAGCTGCTCATTGA
  CCCCGAGGACGACGTCCGCGACAACATCCTCAAGTATGACGAGGAAGGCGGTGGCGAGGA
  GGACCAGGACTACGACCTCAGCCAGCTGCAGCAGCCGGAAGCCATGGGGCACGTGCCAAG
  CAAAGCCCCTGGCGTGCGTCGCGTGGATGAGCGGCCGGTGGGCGCTGAGCCCCAGTACCC
  GATCAGGCCCATGGTGCCGCACCCAGGCGACATCGGTGACTTCATCAATGAGGGACTCCG
  CGCTGCTGACAACGACCCCACGGCACCCCCCTATGACTCCCTGCTGGTCTTCGACTACGA
  GGGGAGCGGCTCCACCGCAGGCTCCGTCAGCTCCCTGAACTCATCCAGTTCCGGGGACCA
  AGACTACGATTACCTCAACGACTGGGGGCCCAGATTCAAGAAGCTGGCGGACATGTATGG
  AGGTGGTGAAGAGGATTGACTGACCTCGCATCTTCGGACCGAAGTGAGAGCCGTGCTCGG
  ACGCCGGAGGAGCAGGACTGAGCAGAGGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001794
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001794.2, NP_001785.2
• RefSeq Size: 3063 bp
• RefSeq ORF: 2751 bp
• Locus ID: 1002
• Cytogenetics: 20q13.33
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.