VE Cadherin (CDH5) (NM_001795) Human Untagged Clone

CAT#: SC119021

CDH5 (untagged)-Human cadherin 5, type 2 (vascular endothelium) (CDH5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDH5
• Synonyms: 7B4; CD144
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001795, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGAGGCTCATGATGCTCCTCGCCACATCGGGCGCCTGCCTGGGCCTGCTGGCAGTGGCAGCAGTGG
  CAGCAGCAGGTGCTAACCCTGCCCAACGGGACACCCACAGCCTGCTGCCCACCCACCGGCGCCAAAAGAG
  AGATTGGATTTGGAACCAGATGCACATTGATGAAGAGAAAAACACCTCACTTCCCCATCATGTAGGCAAG
  ATCAAGTCAAGCGTGAGTCGCAAGAATGCCAAGTACCTGCTCAAAGGAGAATATGTGGGCAAGGTCTTCC
  GGGTCGATGCAGAGACAGGAGACGTGTTCGCCATTGAGAGGCTGGACCGGGAGAATATCTCAGAGTACCA
  CCTCACTGCTGTCATTGTGGACAAGGACACTGGCGAAAACCTGGAGACTCCTTCCAGCTTCACCATCAAA
  GTTCATGACGTGAACGACAACTGGCCTGTGTTCACGCATCGGTTGTTCAATGCGTCCGTGCCTGAGTCGT
  CGGCTGTGGGGACCTCAGTCATCTCTGTGACAGCAGTGGATGCAGACGACCCCACTGTGGGAGACCACGC
  CTCTGTCATGTACCAAATCCTGAAGGGGAAAGAGTATTTTGCCATCGATAATTCTGGACGTATTATCACA
  ATAACGAAAAGCTTGGACCGAGAGAAGCAGGCCAGGTATGAGATCGTGGTGGAAGCGCGAGATGCCCAGG
  GCCTCCGGGGGGACTCGGGCACGGCCACCGTGCTGGTCACTCTGCAAGACATCAATGACAACTTCCCCTT
  CTTCACCCAGACCAAGTACACATTTGTCGTGCCTGAAGACACCCGTGTGGGCACCTCTGTGGGCTCTCTG
  TTTGTTGAGGACCCAGATGAGCCCCAGAACCGGATGACCAAGTACAGCATCTTGCGGGGCGACTACCAGG
  ACGCTTTCACCATTGAGACAAACCCCGCCCACAACGAGGGCATCATCAAGCCCATGAAGCCTCTGGATTA
  TGAATACATCCAGCAATACAGCTTCATCGTCGAGGCCACAGACCCCACCATCGACCTCCGATACATGAGC
  CCTCCCGCGGGAAACAGAGCCCAGGTCATTATCAACATCACAGATGTGGACGAGCCCCCCATTTTCCAGC
  AGCCTTTCTACCACTTCCAGCTGAAGGAAAACCAGAAGAAGCCTCTGATTGGCACAGTGCTGGCCATGGA
  CCCTGATGCGGCTAGGCATAGCATTGGATACTCCATCCGCAGGACCAGTGACAAGGGCCAGTTCTTCCGA
  GTCACAAAAAAGGGGGACATTTACAATGAGAAAGAACTGGACAGAGAAGTCTACCCCTGGTATAACCTGA
  CTGTGGAGGCCAAAGAACTGGATTCCACTGGAACCCCCACAGGAAAAGAATCCATTGTGCAAGTCCACAT
  TGAAGTTTTGGATGAGAATGACAATGCCCCGGAGTTTGCCAAGCCCTACCAGCCCAAAGTGTGTGAGAAC
  GCTGTCCATGGCCAGCTGGTCCTGCAGATCTCCGCAATAGACAAGGACATAACACCACGAAACGTGAAGT
  TCAAATTCATCTTGAATACTGAGAACAACTTTACCCTCACGGATAATCACGATAACACGGCCAACATCAC
  AGTCAAGTATGGGCAGTTTGACCGGGAGCATACCAAGGTCCACTTCCTACCCGTGGTCATCTCAGACAAT
  GGGATGCCAAGTCGCACGGGCACCAGCACGCTGACCGTGGCCGTGTGCAAGTGCAACGAGCAGGGCGAGT
  TCACCTTCTGCGAGGATATGGCCGCCCAGGTGGGCGTGAGCATCCAGGCAGTGGTAGCCATCTTACTCTG
  CATCCTCACCATCACAGTGATCACCCTGCTCATCTTCCTGCGGCGGCGGCTCCGGAAGCAGGCCCGCGCG
  CACGGCAAGAGCGTGCCGGAGATCCACGAGCAGCTGGTCACCTACGACGAGGAGGGCGGCGGCGAGATGG
  ACACCACCAGCTACGATGTGTCGGTGCTCAACTCGGTGCGCCGCGGCGGGGCCAAGCCCCCGCGGCCCGC
  GCTGGACGCCCGGCCTTCCCTCTATGCGCAGGTGCAGAAGCCACCGAGGCACGCGCCTGGGGCACACGGA
  GGGCCCGGGGAGATGGCAGCCATGATCGAGGTGAAGAAGGACGAGGCGGACCACGACGGCGACGGCCCCC
  CCTACGACACGCTGCACATCTACGGCTACGAGGGCTCCGAGTCCATAGCCGAGTCCCTCAGCTCCCTGGG
  CACCGACTCATCCGACTCTGACGTGGATTACGACTTCCTTAACGACTGGGGACCCAGGTTTAAGATGCTG
  GCTGAGCTGTACGGCTCGGACCCCCGGGAGGAGCTGCTGTATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001795 unedited
  AGCCACTCGGGGGTTTNGTTAAACGNACTCTCTATAGGGNACGGCCGCGNCACTTCGGCA
  CGNAGNCAGTCCCTGGNACGGNACAGTGCAGTTCCAACGGNAACANAAACATCCCTCAGC
  CCACAGGCACGATCTGTTCCTCCTGGGAAGATGCAGAGGCTCATGATGCTCCTCGCCACA
  TCGGGCGCCTGCCTGGGCCTGCTGGCAGTGGCAGCAGTGGCAGCAGCAGGTGCTATTTTT
  GCCCAACGGGACACCCACAGCCTGCTGCCCACCCACCGGCGCCAAAAGAGAGATTGGATT
  TGGAACCAGATGCACATTGATGAAGAGAAAAACACCTCACTTCCCCATCATGTAGGCAAG
  ATCAAGTCAAGCGTGAGTCGCAAGAATGCCAAGTACCTGCTCAAAGGAGAATATGTGGGC
  AAGGTCTTCCGGGTCGATGCAGAGACAGGAGACGTGTTCGCCATTGAGAGGCTGGACCGG
  GAGAATATCTCAGAGTACCACCTCACTGCTGTCATTGTGGACAAGGACACTGGTGAAAAC
  CTGGAGACTCCTTCCAGCTTCACCATCAAAGTTCATGACGTGAACGACAACTGGCCTGTG
  TTCACGCATCGGTTGTTCAATGCGTCCGTGCCTGAGTCGTCGGCTGTGGGGACCTCAGTC
  ATCTCTGTGACAGCAGTGGATGCAGACGACCCCACTGTGGGAGACCACGCCTCTGTCATG
  TACCAAATCCTGAAGGGGAAAGAGTATTTTGCCATCGATAATTCTGGACGTATTATCACA
  ATAACGAATAGCTTGNACCGAGAGTATGCAGCCATGTATGATATCGTGGTGGAAGCGCGA
  GATGCCCATGGCCTCCGGNGGGACTCGGGCACGGCCACCGTGCTGGTCACTCTGCAAGAA
  TCAATGACATCTTCCCCTTCTGCACCAGACAAGTACACATTTGTCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001795 unedited
  NTTAGCTCTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCCCA
  GGCTAAAGATTTTTCTTTATTTAGGTATAAAAAAAACAGTACTTGTCATGCACCAGTTTG
  GCCAATATATAGACATTATCTGTAGAGCAATTACTACAATTATAGAAAATAGAAGGTTAC
  AGGAATTGTTTTTCCAACTAAAAAACCTGAATTAATATTGTTAACCTAAATAACAACCTG
  CTTATTATGCAAAAACCCTTATTCTATATGTCTGTGAGAGCTAGAAACTTGTAAGTATCA
  TTAGTAAAAAAATCATTATAAGAAGCTAGTTATTGGTTAAAACAAACAAGTCAGTGTTAT
  CTACAATCCCTTGCAGTGTGAGGGGCTTTGCCCAGAGTGGGAACCTCGGTCCATGACGAA
  GGGTGAGCTTGGTGTCTCCTTCCCTGCCCCTCTCTGTTGACTGATGCCACTTCTCCAAGG
  TGTGTTTGCAGTGAAACGTTCCAGTGGAAGATGCATGGGTGACCACAGAGGTTGGGCAGG
  GCAGGGCCTTGCCTTCTGCAAGGTGTGCCTGAGGGTCAGTTCCCTGAGATGTGACAACAG
  CGAGGTGTAAAGACACATGGCTCGGCTCCGTCATGCAGTCATGAGTGGGTTTGGACATTG
  TCCAGTTTTGCTAAAGGCCACATCTTGGGTTCCTCTAAACCAAGGGATAAGGAGTAGACA
  GAGAAAAGAAAGAGAGCCTGGATTGGCGACCANGTGAGGCAGAGAAGGGGAGACGTCTTC
  TGCTCCGGAACATCTGCCCCTTCTCAGGCAAACTGGNAGGGTGCTGANGAGTCTCAAAGC
  AGGTCTCAGCTCTTCCAGGCCTCTCCAAAGGGGGTGGTGGTGGAAGGTGGCCCTCACCCT
  GGGATCTGGCACTCCTGATCTTCACCTGCCCTCCAGAATGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001795
• Insert Size: 4200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001795.2, NP_001786.1
• RefSeq Size: 4134 bp
• RefSeq ORF: 4134 bp
• Locus ID: 1003
• Cytogenetics: 16q21
• Domains: Cadherin_C_term, CA
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
• Gene Summary: 'This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).