CD34 (NM_001773) Human Untagged Clone

CAT#: SC119045

CD34 (untagged)-Human CD34 molecule (CD34), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD34
• Synonyms: CD34 antigen; CD34 molecule; hematopoietic progenitor cell antigen CD34; OTTHUMP00000034733; OTTHUMP00000034734
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001773, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGGTCCGCAGGGGCGCGCGCGCAGGGCCCAGGATGCCGCGGGGCTGGACCGCGCTTTGCTTGCTGA
  GTTTGCTGCCTTCTGGGTTCATGAGTCTTGACAACAACGGTACTGCTACCCCAGAGTTACCTACCCAGGG
  AACATTTTCAAATGTTTCTACAAATGTATCCTACCAAGAAACTACAACACCTAGTACCCTTGGAAGTACC
  AGCCTGCACCCTGTGTCTCAACATGGCAATGAGGCCACAACAAACATCACAGAAACGACAGTCAAATTCA
  CATCTACCTCTGTGATAACCTCAGTTTATGGAAACACAAACTCTTCTGTCCAGTCACAGACCTCTGTAAT
  CAGCACAGTGTTCACCACCCCAGCCAACGTTTCAACTCCAGAGACAACCTTGAAGCCTAGCCTGTCACCT
  GGAAATGTTTCAGACCTTTCAACCACTAGCACTAGCCTTGCAACATCTCCCACTAAACCCTATACATCAT
  CTTCTCCTATCCTAAGTGACATCAAGGCAGAAATCAAATGTTCAGGCATCAGAGAAGTGAAATTGACTCA
  GGGCATCTGCCTGGAGCAAAATAAGACCTCCAGCTGTGCGGAGTTTAAGAAGGACAGGGGAGAGGGCCTG
  GCCCGAGTGCTGTGTGGGGAGGAGCAGGCTGATGCTGATGCTGGGGCCCAGGTATGCTCCCTGCTCCTTG
  CCCAGTCTGAGGTGAGGCCTCAGTGTCTACTGCTGGTCTTGGCCAACAGAACAGAAATTTCCAGCAAACT
  CCAACTTATGAAAAAGCACCAATCTGACCTGAAAAAGCTGGGGATCCTAGATTTCACTGAGCAAGATGTT
  GCAAGCCACCAGAGCTATTCCCAAAAGACCCTGATTGCACTGGTCACCTCGGGAGCCCTGCTGGCTGTCT
  TGGGCATCACTGGCTATTTCCTGATGAATCGCCGCAGCTGGAGCCCCACAGGAGAAAGGCTGGAGCTGGA
  ACCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001773 unedited
  GTTCAAAATTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGTAGGAGCCGC
  GCGGGAAGGATGCTGGTCCGCAGGGCGCGCGCGCAGGGCCCAGGATGCCGCGGGGCTGGA
  CCGCGCTTTGCTTGCTGAGTTTGCTGCCTTCTGGGTTCATGAGTCTTGACAACAACGGTA
  CTGCTACCCCAGAGTTACCTACCCAGGGAACATTTTCAAATGTTTCTACAAATGTATCCT
  ACCAAGAAACTACAACACCTAGTACCCTTGGAAGTACCAGCCTGCACCCTGTGTCTCAAC
  ATGGCAATGAGGCCACAACAAACATCACAGAAACGACAGTCAAATTCACATCTACCTCTG
  TGATAACCTCAGTTTATGGAAACACAAACTCTTCTGTCCAGTCACAGACCTCTGTAATCA
  GCACAGTGTTCACCACCCCAGCCAACGTTTCAACTCCAGAGACAACCTTGAAGCCTAGCC
  TGTCACCTGGAAATGTTTCAGACCTTTCAACCACTAGCACTAGCCTTGCAACATCTCCCA
  CTAAACCCTATACATCATCTTCTCCTATCCTAAGTGACATCAAGGCAGAAATCAAATGTT
  CAGGCATCAGAGAAGTGAAATTGACTCAGGGCATCTGCCTGGAGCAAAATAAGACCTCCA
  GCTGTGCGGAGTTTAAAAAGGACAGGGGAGAGGGCCTGGCCCGAGTGCTGTGTGGGGAGG
  AGCANGCTGATGCTGNATGCTGGGCCCAGGTATGCTCCCTGCTCCTTGCCCAGTCTGAGG
  TGAGGCCTCAGTGTCTACTGCTGGTCTTGGCCAACAGAACAGAAATTTCCAGCAAACTCC
  AACTTATGANAAAGCACCAATCTGACCTGAANAAAGCTGGGGATCCCCTAGATTNCACTG
  AGCAGAATGNNTGCAGCCACCAGAGCTATTCCCAAAGACCCCTGATGCACTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001773 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTTCAGTATCACTTTAA
  TTGCAGTATTTAAACACATCACTTTGTATTCAGAAAAAAATCTACCCAATACTCTCTTCT
  CTGGAAATTTCTATTTCCAACCGTCATTGAAACCAGGATCCCTGCTCAACCCCTCTGGAA
  AGAAATCAACAGCAAACAAGGACCTGGGTCACCCACAGAAGAGGCAGCTGGTGATAAGGG
  TTAGGAGCTGATCTGGGCTATGACCATATGGGGGTGCAGAGCAAGGAAGGGGCTCAGGGT
  GAGGGAGGCAGAGACAGAAAAGCATCTGTTGGGGGACTGAGGCAACAGCTCAACCCAGGA
  GTCGGCAGGAGGGAGGAGGAAGCCATGGAGATCAGAGGATAAGGCTAGGATCCAGCCTCA
  GAGGAAGAGGATTTTTCGGAAGAGTACAGGTGAGAGGCTCAGCTCCAAGACCTGGTCCCA
  TGGTCCTGCCTACTTTGATCCAGAGGGAGAAAAAGGAGCCCAAAAAGAGTTTCTGTGTGG
  TACCAGAGAGACTACAACTGAGCTGTCTGTACTAAAACTTGCGAGGCCTGTTCTAGGCTC
  CAGCCAGCAAACTGCGCCCGACTTCTGCCAATGGTAGAGGGGGCACATGGAGCTTAGTGG
  AACTTAAAGAACAACGGAGGCACTGGCGACCCCCCCCCAGTGTTTTCGAGATAGATGACG
  GAAGCGCCTCAAGCCCCCCCTCTTTCTGGCTTTTCATTACACGAGAGCCGTGCAAAAGGC
  CCTTTCGCTTACTCCAAGTTGCGAACGACATCCCGGCACCAACGGCAAAACCAAAGCTTC
  TGGAGAAAATGTACCTAAAAACCCACTCTCCCCGGGGGCTCTGATGCGCAAAAAGGACGC
  CCATTCCTCTTTCCTTTCCCCTCCGCCCTTTTGTATTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001773
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001773.1, NP_001764.1
• RefSeq Size: 2657 bp
• RefSeq ORF: 987 bp
• Locus ID: 947
• Cytogenetics: 1q32.2
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Hematopoietic cell lineage
• Gene Summary: 'The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus compared to isoform a.