Catalase (CAT) (NM_001752) Human Untagged Clone

CAT#: SC119081

CAT (untagged)-Human catalase (CAT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAT
• Synonyms: MGC138422; MGC138424
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119081 sequence for NM_001752 edited (data generated by NextGen Sequencing)
  ATGGCTGACAGCCGGGATCCCGCCAGCGACCAGATGCAGCACTGGAAGGAGCAGCGGGCC
  GCGCAGAAAGCTGATGTCCTGACCACTGGAGCTGGTAACCCAGTAGGAGACAAACTTAAT
  GTTATTACAGTAGGGCCCCGTGGGCCCCTTCTTGTTCAGGATGTGGTTTTCACTGATGAA
  ATGGCTCATTTTGACCGAGAGAGAATTCCTGAGAGAGTTGTGCATGCTAAAGGAGCAGGG
  GCCTTTGGCTACTTTGAGGTCACACATGACATTACCAAATACTCCAAGGCAAAGGTATTT
  GAGCATATTGGAAAGAAGACTCCCATCGCAGTTCGGTTCTCCACTGTTGCTGGAGAATCG
  GGTTCAGCTGACACAGTTCGGGACCCTCGTGGGTTTGCAGTGAAATTTTACACAGAAGAT
  GGTAACTGGGATCTCGTTGGAAATAACACCCCCATTTTCTTCATCAGGGATCCCATATTG
  TTTCCATCTTTTATCCACAGCCAAAAGAGAAATCCTCAGACACATCTGAAGGATCCGGAC
  ATGGTCTGGGACTTCTGGAGCCTACGTCCTGAGTCTCTGCATCAGGTTTCTTTCTTGTTC
  AGTGATCGGGGGATTCCAGATGGACATCGCCACATGAATGGATATGGATCACATACTTTC
  AAGCTGGTTAATGCAAATGGGGAGGCAGTTTATTGCAAATTCCATTATAAGACTGACCAG
  GGCATCAAAAACCTTTCTGTTGAAGATGCGGCGAGACTTTCCCAGGAAGATCCTGACTAT
  GGCATCCGGGATCTTTTTAACGCCATTGCCACAGGAAAGTACCCCTCCTGGACTTTTTAC
  ATCCAGGTCATGACATTTAATCAGGCAGAAACTTTTCCATTTAATCCATTCGATCTCACC
  AAGGTTTGGCCTCACAAGGACTACCCTCTCATCCCAGTTGGTAAACTGGTCTTAAACCGG
  AATCCAGTTAATTACTTTGCTGAGGTTGAACAGATAGCCTTCGACCCAAGCAACATGCCA
  CCTGGCATTGAGGCCAGTCCTGACAAAATGCTTCAGGGCCGCCTTTTTGCCTATCCTGAC
  ACTCACCGCCATCGCCTGGGACCCAATTATCTTCATATACCTGTGAACTGTCCCTACCGT
  GCTCGAGTGGCCAACTACCAGCGTGATGGCCCGATGTGCATGCAGGACAATCAGGGTGGT
  GCTCCAAATTACTACCCCAACAGCTTTGGTGCTCCGGAACAACAGCCTTCTGCCCTGGAG
  CACAGCATCCAATATTCTGGAGAAGTGCGGAGATTCAACACTGCCAATGATGATAACGTT
  ACTCAGGTGCGGGCATTCTATGTGAACGTGCTGAATGAGGAACAGAGGAAACGTCTGTGT
  GAGAACATTGCCGGCCACCTGAAGGATGCACAAATTTTCATCCAGAAGAAAGCGGTCAAG
  AACTTCACTGAGGTCCACCCTGACTACGGGAGCCACATCCAGGCTCTTCTGGACAAGTAC
  AATGCTGAGAAGCCTAAGAATGCGATTCACACCTTTGTGCAGTCCGGATCTCACTTGGCG
  GCAAGGGAGAAGGCAAATCTGTGA
  
  Clone variation with respect to NM_001752.3
  1167 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001752 unedited
  GGTTGNAATTTGTATACGACTCACTATAGGGCGGCCGCGCAATTCGCACGAGGACGAGCC
  GAGGCCTCCTGCAGTGTTCTGCACAGCAAACCGCACGCTATGGCTGACAGCCGGGATCCC
  GCCAGCGACCAGATGCAGCACTGGAAGGAGCAGCGGGCCGCGCAGAAAGCTGATGTCCTG
  ACCACTGGAGCTGGTAACCCAGTAGGAGACAAACTTAATGTTATTACAGTAGGGCCCCGT
  GGGCCCCTTCTTGTTCAGGATGTGGTTTTCACTGATGAAATGGCTCATTTTGACCGAGAG
  AGAATTCCTGAGAGAGTTGTGCATGCTAAAGGAGCAGGGGCCTTTGGCTACTTTGAGGTC
  ACACATGACATTACCAAATACTCCAAGGCAAAGGTATTTGAGCATATTGGAAAGAAGACT
  CCCATCGCAGTTCGGTTCTCCACTGTTGCTGGAGAATCGGGTTCAGCTGACACAGTTCGG
  GACCCTCGTGGGTTTGCAGTGAAATTTTACACAGAAGATGGTAACTGGGATCTCGTTGGA
  AATAACACCCCCATTTTCTTCATCAGGGATCCCATATTGTTTCCATCTTTTATCCACAGC
  CAAAAGAGAAATCCTCAGACACATCTGAAGGATCCGGACATGGTCTGGGACTTCTGGAGC
  CTACGTCCTGAGTCTCTGCATCACGTTTCTTTCTTGTTCAGTGATCCGGGGATTCCAGAT
  GGACATCGCCACATGAATGGATATGGATCACATACTCTCCAGCCTGTTTAATGCCAAATG
  GGGAGGCAGTTTATTGCAAATTCCATTATAAGACTGACCACGGCATCAAAAACCTTTCTG
  TTGAAGATCCGGCGAGACTTTCCCANGAAGAATCTGACTATGGCATCCGGGGATCTTTTT
  ACCGCCATTGCCCCAGNAAAGTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001752 unedited
  NNNTTTTTAGCTATGGACCCGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTACGGACAGATTATTTATTGGAAAAATGTAAGCAATTACA
  AATTGATCTGTTGGGAAATCAGGGCTGAATTAACATTAATGTAACTCCAGGGAAAAAAAT
  GAATTTGATCAATTATTATCATTAATAAACATTAAGCCATGACGGGGCTCAAGTTATCTT
  CTGATGACACTGCTGTATTAAATGTACTTTTCCTATGAAAACCTTAAAAAAATAAAAATT
  TCTGAGGAAAACATCTTTATCTTTTCTTTTTATATATTTATAGCCATATTTTAATATAAT
  AGGCCATGAGGGGATATGTAAACATACATAATTTCAACAAATTTTGTCAAATAAAATCAT
  ATTTCTAGCTAGAAACTTGCCTTCTAATCATTTTAAATGAATAATCCAATCATCCGTCAA
  AACAAATTTTTTTTTTAAATGACTGTTAAATCCTAACCTTCATTTTCCCCTGGGGAAATA
  GCATTAAAATTGTTACACAATCTGCTATAGAAAGTGGGATTCTCTATTTTGAAGCCACTA
  GCTTGCATTTGCACATCTAGCACAGGAGAATCTTCATCCAGTGATGAGCGGGTTACACCG
  GATGAACGCTAAGCTTCGCTGCACAGGTGCAGGGCCCCGGCCTCACAGATTTGCCTTCTC
  CCTTGCCGCCAAGTGAGATCCGGACTGCACAAAGGTGTGAATCGCATTCTAAGGCTTTTC
  AGCATTGCACTTGCCCAAAAAGCCTGAATGTGCCTCCCGTATCCAGGTGGACCTCAGTGA
  AGTCTTGACCGTTTTCTCTGGAAGAAAATTTGGCATCCTTCAGGTGCCCGCAATGTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001752
• Insert Size: 2150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001752.2, NP_001743.1
• RefSeq Size: 2305 bp
• RefSeq ORF: 1584 bp
• Locus ID: 847
• Cytogenetics: 11p13
• Domains: catalase
• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Metabolic pathways, Methane metabolism, Tryptophan metabolism
• Gene Summary: 'This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]'