ATP2B2 (NM_001683) Human Untagged Clone

CAT#: SC119107

ATP2B2 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP2B2
• Synonyms: PMCA2; PMCA2a; PMCA2i
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119107 sequence for NM_001683 edited (data generated by NextGen Sequencing)
  ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCAT
  GGGGGCGAGTTCGGGTGCACAATGGAGGAGCTCCGCTCCCTCATGGAGCTGCGGGGCACT
  GAGGCTGTGGTCAAGATCAAGGAGACTTATGGGGACACCGAAGCCATCTGCCGGCGCCTC
  AAAACCTCACCTGTTGAAGGTTTGCCGGGCACCGCTCCAGACCTGGAAAAGAGAAAGCAA
  ATTTTTGGGCAAAACTTTATACCTCCAAAGAAGCCAAAAACCTTCCTGCAGCTCGTGTGG
  GAGGCGCTGCAGGACGTGACGCTCATCATCCTGGAGATTGCCGCCATCATCTCCCTGGGG
  CTGTCCTTCTACCACCCGCCCGGCGAGGGCAACGAAGGATGTGCGACGGCCCAGGGTGGG
  GCAGAGGATGAAGGAGAGGCAGAGGCAGGTTGGATCGAGGGGGCCGCCATTCTCCTCTCA
  GTTATCTGTGTGGTCCTGGTCACGGCCTTCAATGACTGGAGCAAAGAGAAACAGTTCCGG
  GGCCTGCAGAGCCGCATCGAGCAGGAACAGAAATTTACCGTGGTCCGGGCTGGCCAGGTG
  GTGCAGATCCCTGTGGCTGAGATCGTGGTTGGGGACATAGCCCAGGTCAAATATGGTGAC
  CTCCTCCCTGCCGACGGCCTCTTCATCCAGGGCAATGACCTCAAGATTGATGAAAGCTCC
  CTAACTGGAGAGTCTGACCAGGTGCGCAAGTCCGTGGACAAGGACCCCATGCTGCTGTCA
  GGAACCCACGTGATGGAGGGCTCAGGACGGATGTTGGTGACTGCTGTGGGTGTGAACTCT
  CAGACTGGCATCATCTTTACCCTCCTGGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGAC
  AAAAAAGCCAAACAACAGGACGGGGCAGCCGCCATGGAGATGCAGCCCCTCAAGAGTGCC
  GAGGGCGGCGACGCTGACGACAGGAAGAAGGCCAGCATGCACAAGAAGGAGAAGTCCGTG
  CTGCAGGGCAAGCTCACCAAGCTGGCTGTGCAGATCGGGAAGGCGGGCTTGGTGATGTCA
  GCCATCACGGTGATCATCCTGGTGCTCTACTTCACTGTGGACACCTTCGTGGTCAACAAG
  AAGCCGTGGCTGCCTGAGTGCACGCCCGTCTACGTGCAGTACTTTGTCAAGTTCTTCATC
  ATTGGCGTGACGGTGCTGGTGGTCGCCGTGCCCGAGGGGCTCCCTCTGGCCGTCACCATC
  TCGTTGGCCTATTCGGTGAAGAAAATGATGAAGGACAACAATCTGGTACGCCACCTGGAT
  GCCTGTGAGACCATGGGCAATGCCACAGCCATCTGCTCAGACAAGACAGGCACGCTGACC
  ACCAATCGCATGACAGTGGTACAGGCCTATGTCGGCGACGTCCACTATAAAGAGATCCCC
  GACCCCAGCTCCATCAACACCAAGACCATGGAGCTGCTGATCAATGCCATCGCCATCAAC
  AGCGCCTACACCACCAAGATTCTGCCCCCAGAGAAGGAGGGCGCCCTGCCTCGGCAGGTG
  GGCAACAAGACGGAGTGCGGCCTGCTGGGCTTCGTGCTGGACCTGAAGCAGGACTACGAG
  CCCGTGCGCAGCCAGATGCCAGAGGAGAAGTTGTACAAAGTGTACACCTTCAACTCCGTG
  CGCAAGTCCATGAGCACTGTCATCAAGCTGCCCGACGAGAGCTTCCGCATGTACAGCAAG
  GGGGCTTCTGAGATCGTGCTCAAGAAGTGCTGCAAAATCCTCAATGGGGCGGGAGAGCCT
  CGTGTCTTCCGGCCCCGCGACCGGGACGAGATGGTAAAGAAGGTGATTGAGCCCATGGCT
  TGCGATGGGCTCCGCACTATCTGCGTGGCCTACCGCGACTTCCCCAGCAGCCCGGAGCCG
  GACTGGGACAATGAGAATGACATCCTCAACGAACTCACCTGCATCTGCGTGGTGGGCATC
  GAGGACCCGGTGCGGCCAGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCGGGCAGGCATC
  ACGGTCCGCATGGTCACTGGCGACAATATCAACACGGCTCGGGCCATCGCCATCAAGTGT
  GGCATCATCCATCCTGGGGAGGACTTTCTGTGCCTCGAGGGCAAGGAGTTCAACAGGAGG
  ATCCGCAACGAGAAGGGGGAGATTGAGCAGGAGCGAATTGACAAGATCTGGCCAAAGCTG
  CGGGTGCTGGCTCGCTCCTCCCCAACGGACAAGCATACCCTGGTTAAAGGCATCATCGAC
  AGCACACACACTGAGCAGCGGCAGGTGGTGGCCGTGACGGGGGACGGGACCAACGACGGG
  CCTGCACTCAAGAAGGCCGACGTGGGCTTCGCCATGGGCATCGCAGGCACTGACGTGGCC
  AAGGAGGCCTCAGACATCATCCTGACAGACGACAATTTCAGCAGCATCGTCAAGGCAGTG
  ATGTGGGGCCGCAACGTCTATGACAGCATCTCCAAATTCTTGCAGTTCCAGCTCACCGTC
  AACGTGGTGGCCGTGATTGTGGCCTTCACAGGCGCCTGCATCACGCAGGACTCCCCTCTG
  AAGGCCGTGCAGATGCTCTGGGTGAACCTCATCATGGACACGTTTGCCTCGCTGGCACTG
  GCCACTGAGCCGCCCACGGAGACCCTGCTGCTGAGGAAGCCGTACGGCCGCAACAAGCCG
  CTCATCTCCAGGACCATGATGAAGAACATCCTGGGCCATGCTGTCTACCAGCTTGCCCTC
  ATCTTCACCCTGCTCTTTGTTGGCGAGAAGATGTTCCAGATCGACAGCGGGAGGAACGCG
  CCCCTGCATTCGCCACCCTCAGAACATTACACCATCATCTTCAACACCTTCGTCATGATG
  CAGCTCTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAATGTCTTTGACGGC
  ATCTTCCGGAACCCCATCTTCTGCACCATCGTGCTGGGCACCTTTGCCATCCAGATAGTG
  ATCGTGCAGTTTGGAGGGAAGCCATTCAGCTGCTCTCCACTGCAGCTGGACCAGTGGATG
  TGGTGCATATTCATTGGGTTAGGAGAGCTCGTTTGGGGCCAGGTCATCGCCACCATCCCG
  ACCAGCAGACTCAAGTTCCTCAAGGAGGCAGGCAGGCTCACACAGAAGGAGGAGATCCCG
  GAGGAGGAGCTCAACGAGGACGTGGAGGAGATCGACCACGCGGAGCGGGAGCTGCGGCGG
  GGCCAGATCCTGTGGTTCCGAGGCCTGAATCGGATCCAGACACAGATCCGCGTCGTGAAG
  GCGTTCCGTAGCTCTCTCTATGAAGGTTTAGAAAAGCCTGAATCTCGAACCTCCATCCAT
  AACTTCATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATT
  GATGACACCGACCTGGAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCATCC
  CTCAACAAGAACAACAGCGCCATCGACAGTGGGATCAACCTGACGACCGACACAAGCAAA
  TCAGCTACCTCTTCAAGTCCAGGGAGCCCCATCCACAGCCTGGAGACGTCGCTTTAG
  
  Clone variation with respect to NM_001683.3
  603 c=>g;1302 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001683 unedited
  TTCTTCCCCCGCCCGNNGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGCGGGCACGCTGGCTGGGCTCAGCCGCGGCACCAGCTGCCGCACC
  GCGATTACGGGGCTGCCCGCCGGGAAACCGTGCAGGATTGGTGGTGATTTTCATAGCCAA
  GAGCCACCACCCCTGACCATGTAGATGCCAGTTCCAGGGAGCAGCATGGGCCCCACTGAA
  TGGAGACTCCTGGGTCTACAGCCCTGAGCCCCTCCGGCCCCTGGACCTCGTCCCACACCG
  GAGGACACCTCTTGGAGCTCACCACCACTGTCACCAGCCCGCCTCGGCCACCCCCACCCC
  CCGGGACCCGGAGTCGGCCGCCTGGTGCCACAGCTGACCAGTGAGGGTGTGCTGAGGACA
  GCCACAAGCAGCCATCACCCGGCAGCCTCTTGTCCAGCGCTGACCCTTGGGCCCAGCCCG
  AGCAAGGACCGCAGCAAACATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCA
  AAGAAATGAGTCGAGCCATGGGGGCGAGTTCGGGTGCACAATGGAGGAGCTCCGCTCCCT
  CATGGAGCTGCGGNGCACTGAGGCTGTGGTCAAGATCAAGGAGACTTATGGGGACACCGA
  AGCCATCTGCCGGCGCCTCAAAACCTCACCTGTTGAAGGTTTGCCGGGCACCGCTCCAGA
  CCTGGGAAAGAGAAAGCANATTTTTGGGCACAACTTTATACCTCCCAAGAAGACANAAAC
  CTTCCTGCAGCTCGTGTGGGAAGCGCTGCNAGACGTGACGCTCATCATCCTGGAGAATGC
  CGCCATCATCTCCCTGGGGCTGTCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001683 unedited
  TATCGCGGCCGCAATTANAGTCTAGTTTTTATTTTTTTTTTTTAAAGAACAGANTGGTGA
  GTTCTTTATTTACAATTATTGTCTAGATAAAAAAATTCATAAAAACAGATGAATGGTCAT
  AAATTATTTACCCCCTAATGGAGTGAACTGCCAAACGTACCCCATGCTGCTAGATATAGG
  ACGCAGAGTGCAAGCTTTATGTACTACAGTGAGTTTTGACAAACCAACCATTTCGTGTGT
  GTGTGTGTGTATGTGTGCAAGTGTGAGAGTGAGGGGGTGCATGTGTGCAAGCATCCTTCA
  TTCCTGACAAGAGAAGACCAACGCTAATGTTGGCATTCTTCCATCGCTCCCTGTTCCCTA
  AGCCAGGCTCCACTGAGAACCCCTCCTGGCTCCGAATGAACATGGAGCATGGTGTGCAAA
  CACAGCTATCCTGATGTCATGGAACGAGGTCACGGACACATGACTGATCAAAGAAGTGGG
  GAGATGGAAAACTGTGAGAAAGGAAACTCCAAAAAACACCATGTTCCAAGGCTGCCCCAT
  GTTTTACACGTGCAGATCTTTCCTTCCTTGTAGGAGAGCAGTGGGCTGGCTTTGGTGGCG
  TTTGTACTGGAATCCAATAAGCACATCTGANGGCTGGTCACCCCTTTGGTTATGCAGTTC
  CTCTCCTGGATACATTCAGAGATACTTCTTCACGACATTCANGTGATGCGCTCTTTGAGG
  TCCTCCTTNCCAGAGGNNATTTGCCAGTGGGATGGGCTGACTTTGAACCCCCAACCTCAA
  GTTTTCACCGNCACCAGATGTANGCCCTCCCAGTGACTATGGCTCTTTTNCCGAGTGCTC
  TCATCTTGTTGTGGAANAATTCTCTGGCCCTGAAAAGGAAGGGGCTTGGCTGACCANNCA
  TCGTGAGGAGTTCANCATGAGAATGTATTTTGCAGAAAGCGGAAATGTTTGGTTTCCTCG
  AAACATGGCATATCGATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001683
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001683.2, NP_001674.2
• RefSeq Size: 6686 bp
• RefSeq ORF: 3597 bp
• Locus ID: 491
• Cytogenetics: 3p25.3
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks three in-frame exons, compared to variant 1. The encoded isoform (2), also known as ZB, is shorter, compared to isoform 1. Variants 2 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.