ATP5PF (NM_001685) Human Untagged Clone

CAT#: SC119111

ATP5J (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (ATP5J), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP5PF
• Synonyms: ATP5; ATP5A; ATP5J; ATPM; CF6; F6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119111 sequence for NM_001685 edited (data generated by NextGen Sequencing)
  ATGATTCTTCAGAGGCTCTTCAGGTTCTCCTCTGTCATTCGGTCAGCCGTCTCAGTCCAT
  TTGCGGAGGAACATTGGTGTTACAGCAGTGGCATTTAATAAGGAACTTGATCCTATACAG
  AAACTCTTTGTGGACAAGATTAGAGAATACAAATCTAAGCGACAGACATCTGGAGGACCT
  GTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAGAGGGAGCTTTTTAAGCTCAAGCAA
  ATGTTTGGTAATGCAGACATGAATACATTTCCCACCTTCAAATTTGAAGATCCCAAATTT
  GAAGTCATCGAAAAACCCCAGGCCTGA
  
  Clone variation with respect to NM_001685.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001685 unedited
  ATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGCTCACCTAGACCCGT
  CCGGTCGCAGACTGTCTCCGAGACGCTTCCTGTCCGAATCAGCATGATTCTTCAGAGGCT
  CTTCAGGTTCTCCTCTGTCATTCGGTCAGCCGTCTCAGTCCATTTGCGGAGGAACATTGG
  TGTTACAGCAGTGGCATTTAATAAGGAACTTGATCCTATACAGAAACTCTTTGTGGACAA
  GATTAGAGAATACAAATCTAAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGA
  GTATCAGCAAGAGCTGGAGAGGGAGCTTTTTAAGCTCAAGCAAATGTTTGGTAATGCAGA
  CATGAATACATTTCCCACCTTCAAATTTGAAGATCCCAAATTTGAAGTCATCGAAAAACC
  CCAGGCCTGAAGAAATAAAGTAAAATTAATCTGGTAATTTGTCACGGATTAGTTGTACAA
  CTAGTTAGAAGTTTCAGAATAAACATGCATTTCATAACTGTCAAATGTTCTTTTAATTCT
  GAGTCCAAATAAATTATTTGGTGATGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCTCGACTTTAAATTG
  CGGCCGGGGCCATAGTTGTTTCCTGACCAAATCCCGGTTGGAATCCCTGTGACCCCTCCC
  CAGGGCCTTTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGCCCTAATAA
  AATAAAGTTGCATCATTTGGCCGGACTAGGGGTCCTCCTATATATTATGGNNGTGAGGGG
  GGTGGTATGGAACCAGGGGCCAAGTTGGGAAAAACACCCTGTAGGGCCTTGCGGGGCCTA
  TGGGGACCCAGCCTGNATTGCAGGGGGCACATCTTGGCTCACTGACATCTCCGCCTCCTG
  GTTTAAGCGATNTTCCTGCTAGCCTTCCAGANTGTGGGNATTCAGCCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001685 unedited
  NGGTTTTCCCAATATTTATTTGGTCTTCGNATTAAAGAACATTTGACAGTTATGAAATGC
  ATGTTTATTCTGAAACTTCTAACTAGTTGTACAACTAATCCGTGACAAATTACCAGATTA
  ATTTTACTTTATTTCTTCAGGCCTGGGGTTTTTCGATGACTTCAAATTTGGGATCTTCAA
  ATTTGAAGGTGGGAAATGTATTCATGTCTGCATTACCAAACATTTGCTTGAGCTTAAAAA
  GCTCCCTCTCCAGCTCTTGCTGATACTCTGAACTAGCATCAACAGGTCCTCCAGATGTCT
  GTCGCTTAGATTTGTATTCTCTAATCTTGTCCACAAAGAGTTTCTGTATAGGATCAAGTT
  CCTTATTAAATGCCACTGCTGTAACACCAATGTTCCTCCGCAAATGGACTGAGACGGCTG
  ACCGAATGACAGAGGAGAACCTGAAGAGCCTCTGAAGAATCATGCTGATTCGGACAGGAA
  GCGTCTCGGAGACAGTCTGCGACCGGACGGGTCTAGGTGAGCTCGTGCCGAATTCGCGGC
  CGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATA
  TAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCNGGGGTTATTACG
  ACATTTTTGGAAAGTTCCCGTTGATTTTTGGTGCCAAAACCAAACTCCCATTTGACGTCC
  AATGGGGGTGGGAGACCTTGAAAAATCCCCGGGGAGTCAAACCCGCTATTCCACGCCCAT
  TTGGTGTACCTGCCAAAACCGCATACACATTGGTAATAGCCATTGACTTATTCCCTAAAT
  TGCCCTGGCCATTAGGAAAGATCCCGTAAGGTCTTTAACCTGGCCTTTATGCGCAGGCGG
  GCCTTTACCGTCATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001685
• Insert Size: 610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001685.4, NP_001676.2
• RefSeq Size: 1167 bp
• RefSeq ORF: 327 bp
• Locus ID: 522
• Cytogenetics: 21q21.3
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes. [provided by RefSeq, Feb 2016]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4, 6 and 7 encode the same isoform (a).