Apolipoprotein CI (APOC1) (NM_001645) Human Untagged Clone

CAT#: SC119124

APOC1 (untagged)-Human apolipoprotein C-I (APOC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APOC1
• Synonyms: Apo-CI; apo-CIB; ApoC-I; apoC-IB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119124 sequence for NM_001645 edited (data generated by NextGen Sequencing)
  ATGAGGCTCTTCCTGTCGCTCCCGGTCCTGGTGGTGGTTCTGTCGATCGTCTTGGAAGGC
  CCAGCCCCAGCCCAGGGGACCCCAGACGTCTCCAGTGCCTTGGATAAGCTGAAGGAGTTT
  GGAAACACACTGGAGGACAAGGCTCGGGAACTCATCAGCCGCATCAAACAGAGTGAACTT
  TCTGCCAAGATGCGGGAGTGGTTTTCAGAGACATTTCAGAAAGTGAAGGAGAAACTCAAG
  ATTGACTCATGA
  
  Clone variation with respect to NM_001645.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001645 unedited
  GCACCAGCAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGCTCC
  CGGTCCTGGTGGTGGTTCTGTCGATCGTCTTGGAAGGCCCAGCCCCAGCCCAGGGGACCC
  CAGACGTCTCCAGTGCCTTGGATAAGCTGAAGGAGTTTGGAAACACACTGGAGGACAAGG
  CTCGGGAACTCATCAGCCGCATCAAACAGAGTGAACTTTCTGCCAAGATGCGGGAGTGGT
  TTTCAGAGACATTTCAGAAAGTGAAGGAGAAACTCAAGATTGACTCATGAGGACCTGAAG
  GGTGACATCCCAGGAGGGGCCTCTGAAATTTCCCACACCCCAGCGCCTGTGCTGAGGACT
  CCCTCCATGTGGCCCCAGGTGCCACCAATAAAAATCCTACAGAAAATTCAAAAAAAAAAA
  AAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGG
  TGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTG
  CCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTC
  TATATATATGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001645 unedited
  GCCACCCACANNNANAACCCCATCACCCACAANNTTTGACTTGNCCGCGCCCGCTTTCTA
  GNGATCGGTTTTTTTTTTTTTTTTTTNGAATTTCTGTATGATTTTTATTGGTGGCACCTG
  GGGCCACATGGAGGGAGTCCTCAGCACAGGCGCTGGGGTGTGGGAAATTTCAGAGGCCCC
  TCCTGGGATGTCACCCTTCAGGTCCTCATGAGTCAATCTTGAGTTTCTCCTTCACTTTCT
  GAAATGTCTCTGAAAACCACTCCCGCATCTTGGCAGAAAGTTCACTCTGTTTGATGCGGC
  TGATGAGTTCCCGAGCCTTGTCCTCCAGTGTGTTTCCAAACTCCTTCAGCTTATCCAAGG
  CACTGGAGACGTCTGGGGTCCCCTGGGCTGGGGCTGGGCCTTCCAAGACGATCGACAGAA
  CCACCACCAGGACCGGGAGCGACAGGAAGAGCCTCATGGCGAGGCCGGAGGGGCACTCTG
  AATCCTTGCTGGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGA
  CGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATT
  TGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAA
  AACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGC
  TATCCACGCCCATTGGTGTACTGCCAAAACCGCATCACCATGGGTATAGCGATGACTAAT
  ACGTAGATGTACTGCCAAGTANGAAAGTCCCGTAAGGCCATGTACTGGGCATAATGCCAG
  GCGGCCCATTTACCGCCATTGACGTCAATAGGGGGCCGAACTTGGCTATGATACACTTGA
  TGTACTGCCAAGTGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001645
• Insert Size: 450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001645.3, NP_001636.1
• RefSeq Size: 464 bp
• RefSeq ORF: 252 bp
• Locus ID: 341
• Cytogenetics: 19q13.32
• Domains: ApoC-I
• Protein Families: Secreted Protein
• Gene Summary: 'This gene encodes a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. The encoded protein plays a central role in high density lipoprotein (HDL) and very low density lipoprotein (VLDL) metabolism. This protein has also been shown to inhibit cholesteryl ester transfer protein in plasma. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Sep 2016]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. This transcript is expressed primarily in the liver. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.