ARF1 (NM_001658) Human Untagged Clone

CAT#: SC119130

ARF1 (untagged)-Human ADP-ribosylation factor 1 (ARF1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARF1
• Synonyms: PVNH8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119130 sequence for NM_001658 edited (data generated by NextGen Sequencing)
  ATGGGGAACATCTTCGCCAACCTCTTCAAGGGCCTTTTTGGCAAAAAAGAAATGCGCATC
  CTCATGGTGGGCCTGGATGCTGCAGGGAAGACCACGATCCTCTACAAGCTTAAGCTGGGT
  GAGATCGTGACCACCATTCCCACCATAGGCTTCAACGTGGAAACCGTGGAGTACAAGAAC
  ATCAGCTTCACTGTGTGGGACGTGGGTGGCCAGGACAAGATCCGGCCCCTGTGGCGCCAC
  TACTTCCAGAACACACAAGGCCTGATCTTCGTGGTGGACAGCAATGACAGAGAGCGTGTG
  AACGAGGCCCGTGAGGAGCTCATGAGGATGCTGGCCGAGGACGAGCTCCGGGATGCTGTC
  CTCCTGGTGTTCGCCAACAAGCAGGACCTCCCCAACGCCATGAATGCGGCCGAGATCACA
  GACAAGCTGGGGCTGCACTCACTACGCCACAGGAACTGGTACATTCAGGCCACCTGCGCC
  ACCAGCGGCGACGGGCTCTATGAAGGACTGGACTGGCTGTCCAATCAGCTCCGGAACCAG
  AAGTGA
  
  Clone variation with respect to NM_001658.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001658 unedited
  ATTTGTATACGACNACTATAGGCGGCCGCGAATTCGGCACGAGGCAACGCCTGGCTNCGG
  ACAGCAGCCTCTGAGGTGTCCCTGGCCAGTGTCCTTCCACCTGTCCACAAGCATGGGGAA
  CATCTTCGCCAACCTCTTCAAGGGCCTTTTTGGCAAAAAAGAAATGCGCATCCTCATGGT
  GGGCCTGGATGCTGCAGGGAAGACCACGATCCTCTACAAGCTTAAGCTGGGTGAGATCGT
  GACCACCATTCCCACCATAGGCTTCAACGTGGAAACCGTGGAGTACAAGAACATCAGCTT
  CACTGTGTGGGACGTGGGTGGCCAGGACAAGATCCGGCCCCTGTGGCGCCACTACTTCCA
  GAACACACAAGGCCTGATCTTCGTGGTGGACAGCAATGACAGAGAGCGTGTGAACGAGGC
  CCGTGAGGAGCTCATGAGGATGCTGGCCGAGGACGAGCTCCGGGATGCTGTCCTCCNTGG
  TGTTCGCCAACAAGCAGGACCTCCCCAACGCCATGAATGCGGCCGAGATCACAGACAAGC
  TGGGGCTGCACTCACTACGCCACAGAACTGGTACATTCANGCCACCTGCGCCACCACGGC
  GACGGGCTTATGAGGACTGGACTGGGCTGTCCAATCAGCTCCGGACCCAGAAGTGACGGC
  GACCCCNCTCCTTNTCACTCTNNNTGCCTCTGNTTACTCTCATGTGGCAACGTCNGNNTC
  GGGGGGGGGAGTGCAAAGCTGCTCCGGGGGTTTTGGCAACCGGGGCATTGACGGGCTGTT
  AATTGGGCAGACCAACCTGGCCAGCTTTNAATTATGAAAAAAAAATTTTTGTTCAGAAAG
  GGAAGNTTTGGGACCCCTAGCATATATCAACTTTTTTTTTTGAAAGAAATACCCCGGTTG
  CTTGCGGAGGAGGGGGGGGCCCCCGGCCCTGCCCAAAAAATTTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001658 unedited
  TATGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  AATAGTTAAGAGATTTTATTCTAATAGCTATAATTACAGTGCTTGTTTGTCGAAATGAAA
  ACTGAAAACAAGTATACAAAACAGTTGATTACTAATCGTGTATTGAAAGCAGTAAGAGGT
  TCCACGACACCAAATAGACCAGTTCTGAGGTTTCCCCAAGATAAATTTAACAGCTCCAGC
  TTCAGTGTTTATCAAAATACAAAAGAAAAAAGTAGAGGTTGTCTTTTTCGATGGCAAATC
  GGACCCTTGCAGGCTGAGGGAGAGAAAGCTACATCACACACAGAGGTGGGGTGCTCCCGA
  GGGGCTGTGGGTCTAGGTGGACCGCCTGCCCGGCTTGCACGCGCTCCTGCTGGTGAGGCC
  CCAGACATCCTGCCAAAGTGTCTGAGCGAGCACGAGTGTTGGGGACGACGGACCCACTCT
  GGCCACACGGCGACCGAGGGACAGATGGGGCCCTGCGTCCCATAGGCTGCCTGAAGGTGG
  GTAGGGCGGCCTGCGGCATAGTGGGGTGGCTGTGGGCTCCCAGCCTGGCCCCTGGGAACC
  GTGGGAGCACAGGGACAAGCACATGGCTATGGAATGCAGGGTGACCCAAGGACAAGCGAG
  TTGCGGGGATCTCTACTGTGACCATGCAAAATTGATCGCAGTCTGCTGCGCCACCACCAC
  CTCATGTTNCCGAGGGGAACAGCTGGGCTGGCGACTGGCATCCCAGCCCGTACTGCAAAT
  TATGCTAGGCCGGGGTCTCCCTTCTGTGTGTTCAGGGTTCTCGACTTGGATTCTTAACTT
  TTTAAAAAGCCCCTGAAGTTGGGGTTAAAACCAACCCCCAAATGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001658
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001658.3, NP_001649.1
• RefSeq Size: 1901 bp
• RefSeq ORF: 546 bp
• Locus ID: 375
• Cytogenetics: 1q42.13
• Domains: RAB, SAR, ARF, arf
• Protein Pathways: Vibrio cholerae infection
• Gene Summary: 'ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (4) has an alternate and shorter 5' UTR, as compared to variant 1.