IL12RB2 (NM_001559) Human Untagged Clone

CAT#: SC119132

IL12RB2 (untagged)-Human interleukin 12 receptor, beta 2 (IL12RB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL12RB2
• Synonyms: RP11-102M16.1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001559 edited
  ATGGCACATACTTTTAGAGGATGCTCATTGGCATTTATGTTTATAATCACGTGGCTGTTG
  ATTAAAGCAAAAATAGATGCGTGCAAGAGAGGCGATGTGACTGTGAAGCCTTCCCATGTA
  ATTTTACTTGGATCCACTGTCAATATTACATGCTCTTTGAAGCCCAGACAAGGCTGCTTT
  CACTATTCCAGACGTAACAAGTTAATCCTGTACAAGTTTGACAGAAGAATCAATTTTCAC
  CATGGCCACTCCCTCAATTCTCAAGTCACAGGTCTTCCCCTTGGTACAACCTTGTTTGTC
  TGCAAACTGGCCTGTATCAATAGTGATGAAATTCAAATATGTGGAGCAGAGATCTTCGTT
  GGTGTTGCTCCAGAACAGCCTCAAAATTTATCCTGCATACAGAAGGGAGAACAGGGGACT
  GTGGCCTGCACCTGGGAAAGAGGACGAGACACCCACTTATACACTGAGTATACTCTACAG
  CTAAGTGGACCAAAAAATTTAACCTGGCAGAAGCAATGTAAAGACATTTATTGTGACTAT
  TTGGACTTTGGAATCAACCTCACCCCTGAATCACCTGAATCCAATTTCACAGCCAAGGTT
  ACTGCTGTCAATAGTCTTGGAAGCTCCTCTTCACTTCCATCCACATTCACATTCTTGGAC
  ATAGTGAGGCCTCTTCCTCCGTGGGACATTAGAATCAAATTTCAAAAGGCTTCCGTGAGC
  AGATGTACCCTTTATTGGAGAGATGAGGGACTGGTACTGCTTAATCGACTCAGATATCGG
  CCCAGTAACAGCAGGCTCTGGAATATGGTTAATGTTACAAAGGCCAAAGGAAGACATGAT
  TTGCTGGATCTGAAACCATTTACAGAATATGAATTTCAGATTTCCTCTAAGCTACATCTT
  TATAAGGGAAGTTGGAGTGATTGGAGTGAATCATTGAGAGCACAAACACCAGAAGAAGAG
  CCTACTGGGATGTTAGATGTCTGGTACATGAAACGGCACATTGACTACAGTAGACAACAG
  ATTTCTCTTTTCTGGAAGAATCTGAGTGTCTCAGAGGCAAGAGGAAAAATTCTCCACTAT
  CAGGTGACCTTGCAGGAGCTGACAGGAGGGAAAGCCATGACACAGAACATCACAGGACAC
  ACCTCCTGGACCACAGTCATTCCTAGAACCGGAAATTGGGCTGTGGCTGTGTCTGCAGCA
  AATTCAAAAGGCAGTTCTCTGCCCACTCGTATTAACATAATGAACCTGTGTGAGGCAGGG
  TTGCTGGCTCCTCGCCAGGTCTCTGCAAACTCAGAGGGCATGGACAACATTCTGGTGACT
  TGGCAGCCTCCCAGGAAAGATCCCTCTGCTGTTCAGGAGTACGTGGTGGAATGGAGAGAG
  CTCCATCCAGGGGGTGACACACAGGTCCCTCTAAACTGGCTACGGAGTCGACCCTACAAT
  GTGTCTGCTCTGATTTCAGAGAACATAAAATCCTACATCTGTTATGAAATCCGTGTGTAT
  GCACTCTCAGGGGATCAAGGAGGATGCAGCTCCATCCTGGGTAACTCTAAGCACAAAGCA
  CCACTGAGTGGCCCCCACATTAATGCCATCACAGAGGAAAAGGGGAGCATTTTAATTTCA
  TGGAACAGCATTCCAGTCCAGGAGCAAATGGGCTGCCTCCTCCATTATAGGATATACTGG
  AAGGAACGGGACTCCAACTCCCAGCCTCAGCTCTGTGAAATTCCCTACAGAGTCTCCCAA
  AATTCACATCCAATAAACAGCCTGCAGCCCCGAGTGACATATGTCCTGTGGATGACAGCT
  CTGACAGCTGCTGGTGAAAGTTCCCACGGAAATGAGAGGGAATTTTGTCTGCAAGGTAAA
  GCCAATTGGATGGCGTTTGTGGCACCAAGCATTTGCATTGCTATCATCATGGTGGGCATT
  TTCTCAACACATTACTTCCAGCAAAAGGTGTTTGTTCTCCTAGCAGCCCTCAGACCTCAG
  TGGTGTAGCAGAGAAATTCCAGATCCAGCAAATAGCACTTGCGCTAAGAAATATCCCATT
  GCAGAGGAGAAGACACAGCTGCCCTTGGACAGGCTCCTGATAGACTGGCCCACGCCTGAA
  GATCCTGAACCGCTGGTCATCAGTGAAGTCCTTCATCAAGTGACCCCAGTTTTCAGACAT
  CCCCCCTGCTCCAACTGGCCACAAAGGGAAAAAGGAATCCAAGGTCATCAGGCCTCTGAG
  AAAGACATGATGCACAGTGCCTCAAGCCCACCACCTCCAAGAGCTCTCCAAGCTGAGAGC
  AGACAACTGGTGGATCTGTACAAGGTGCTGGAGAGCAGGGGCTCCGACCCAAAGCCAGAA
  AACCCAGCCTGTCCCTGGACGGTGCTCCCAGCAGGTGACCTTCCCACCCATGATGGCTAC
  TTACCCTCCAACATAGATGACCTCCCCTCACATGAGGCACCTCTCGCTGACTCTCTGGAA
  GAACTGGAGCCTCAGCACATCTCCCTTTCTGTTTTCCCCTCAAGTTCTCTTCACCCACTC
  ACCTTCTCCTGTGGTGATAAGCTGACTCTGGATCAGTTAAAGATGAGGTGTGACTCCCTC
  ATGCTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001559 unedited
  GGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCGGAGAGC
  GCGACACGTGCGGCCCAGAACACCGGGGCCACCCGGTCCCCGCAGGCCCGGGACCGCGCC
  CGCTGGCAGGCGACACGTGGTCACGGTGATCCATTTGTAAAGTCGGGAATAAATGACCTC
  TGAAGTGTTGTCTGTATATTGATCTGCTACCAGTAAAACATATCTCTGAAGAATACGGAG
  TTCTATACCAGAGTTGATTGCTGATGGCACATACTTTTAGAGGATGCTCATTGGCATTTA
  TGTTTATAATCACGTGGCTGTTGATTAAAGCAAAAATAGATGCGTGCAAGAGAGGCGATG
  TGACTGTGAAGCCTTCCCATGTAATTTTACTTGGATCCACTGTCAATATTACATGCTCTT
  TGAAGCCCAGACAAGGCTGCTTTCACTATTCCAGACGTAACAAGTTAATCCTGTACAAGT
  TTGACAGAAGAATCAATTTTCACCATGGCCACTCCCTCAATTCTCAAGTCACAGGTCTTC
  CCCTTGGTACAACCTTGTTTGTCTGCAAACTCTGCCTGTATCAATAGTGATGAAATTCAA
  ATATGTGGAGCAGAGATCTTCGTTGGTGTTGCTCCAGAACAGCCTCAAAATTTATCCTGC
  ATACAGAAGGGAGAACAGGGGACTGTGGCCTGCACCTGGGAAAGAGGACGAGACACCCAC
  TTATACACTGAGTATACTCTACAGCTAAGTGGACCAAAAAATTTAACCTGGCAGAAGCAA
  TGTAAAGACATTTATTGTGACTATTTGGACTTTGGAATCAACCTCANCCCTGAATCACCT
  GAAATCCATTTCACAGCCCAAGTTACTGCTACTCATAGTCCTTGGAAGCTCCTCTTTACT
  TCCTTCACATTCACATTCTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001559 unedited
  TAGCTTTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTTTTT
  GAGACAGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCTCACTGCAAGCCCCACC
  TCCCAGGTTCATGTCATTCTCCTCCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCC
  GCCATCACGCCCAGTTAATTTTTTTTAAAAATATTTTTAGTAGAGATGGGGTTTCACCAT
  GTTAGCCAGAATGGTCTCGATCTCCTGACCTCATGATCCGCCTGCCTCAGCCTCCCAAAG
  TGCTGGGATTACAGGCATGAGCTACCACGCCCGGCCAGAAAAAGGTACATTCTTAGACTT
  CTGCAAATGAGACCAGTCATGACCACCTTGGGGGCCCTTTTACATTGGTGTTTACCAGGG
  TCTTTTATGTCTTCATCTATAGAACTAGGTTGATTTGTTTTGAAGGCTCTCAATAATCTA
  GGAGCCATCTAGAGCTGTGCACTCCAATACAGTAGCCACTAGCCATGTGTGGCGATTTAA
  ATTTACAATCAAAATTTAATTCCTCAGTCAAGCTAAACACATTCCAAGTGATTAACAGCC
  AATGTGCCTAGTGTCTAATGTACTGGACAGTGAGTGCAGAGGTAGAACATTTCCATCATC
  TCAGGAAGTTCTGTTGAACAGTACTGATCTGCTGTGTACAGCCTGCTGTGTACCAGGTGC
  TTTAGAAATATCATTGCATGAATGCCCTCTAGACTGTATGCCATTACTACTAAGAGCTGC
  GGCTTCTACCTCTCTCCTCAATTCCCTTTCTGCCCAGCTACCCAGAAAGTACCTAGGATT
  CAGCTGGAGTCTTTAGTCCCCCAGTGAAGAGTAATTTAAGCCCTGGGAAGCAAATGAGCA
  CTGAGGAAACCACTCCAGTCCTAAACTCCCCAAGCTGTTGCCGGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001559
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001559.2, NP_001550.1
• RefSeq Size: 4040 bp
• RefSeq ORF: 2589 bp
• Locus ID: 3595
• Cytogenetics: 1p31.3
• Domains: FN3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Gene Summary: 'The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012]'
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.