INPP5F (OCRL) (NM_001587) Human Untagged Clone

CAT#: SC119145

OCRL (untagged)-Human oculocerebrorenal syndrome of Lowe (OCRL), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OCRL
• Synonyms: INPP5F; LOCR; NPHL2; OCRL-1; OCRL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001587 edited
  ATGGAGCCGCCGCTCCCGGTCGGAGCCCAGCCGCTTGCCACTGTCGAGGGTATGGAGATG
  AAGGGTCCTCTCCGGGAGCCCTGCGCCCTGACCCTAGCCCAGAGGAACGGGCAATATGAG
  TTAATAATCCAGTTGCATGAGAAGGAACAGCATGTTCAAGATATCATTCCTATAAATAGC
  CACTTCAGATGTGTTCAAGAAGCAGAAGAAACTCTTTTGATTGACATAGCTTCTAACAGT
  GGCTGCAAAATTCGGGTTCAGGGGGACTGGATCAGAGAGCGCCGCTTTGAAATCCCTGAT
  GAGGAACACTGTTTGAAGTTCCTCTCAGCTGTCCTTGCTGCTCAGAAAGCTCAGTCACAG
  CTTCTTGTTCCAGAGCAAAAGGACTCATCTAGCTGGTACCAGAAATTAGACACTAAGGAC
  AAACCTTCTGTTTTTTCAGGGCTTCTTGGATTTGAAGACAATTTTTCTTCTATGAATTTG
  GACAAGAAAATAAATTCACAAAATCAGCCTACTGGGATTCATCGGGAACCCCCACCTCCA
  CCCTTTTCAGTGAATAAAATGCTTCCACGTGAAAAAGAAGCTTCTAACAAGGAGCAGCCC
  AAAGTGACCAACACCATGCGGAAGCTCTTTGTACCAAATACCCAATCTGGGCAGCGGGAG
  GGTCTCATCAAACATATCCTGGCAAAGCGAGAGAAAGAATATGTCAACATTCAGACTTTC
  AGATTTTTTGTTGGAACTTGGAATGTGAATGGCCAGTCTCCAGATAGCGGGTTAGAACCT
  TGGCTGAACTGTGATCCCAATCCTCCTGATATCTACTGCATTGGATTCCAAGAACTGGAC
  TTGAGCACAGAAGCCTTCTTCTACTTTGAATCTGTGAAGGAACAAGAATGGTCCATGGCT
  GTAGAGAGAGGTTTGCATTCCAAAGCCAAGTATAAGAAAGTTCAACTGGTGCGCCTTGTT
  GGGATGATGCTTCTTATATTTGCCAGAAAGGATCAGTGTCGATACATTCGTGATATTGCT
  ACAGAAACAGTTGGAACTGGAATCATGGGGAAAATGGGAAACAAAGGTGGGGTAGCTGTG
  AGATTTGTATTTCACAACACCACCTTTTGCATTGTCAATTCCCATCTGGCTGCACACGTG
  GAGGACTTTGAGAGAAGGAATCAAGATTATAAGGACATTTGTGCGAGAATGAGTTTTGTG
  GTCCCAAATCAGACCCTCCCGCAGTTGAACATCATGAAACATGAGGTTGTCATTTGGTTG
  GGAGATTTGAATTATAGACTTTGCATGCCTGATGCCAATGAGGTGAAAAGTCTTATTAAT
  AAGAAAGACCTTCAGAGACTCTTGAAATTCGACCAGCTAAATATTCAGCGCACACAGAAA
  AAAGCTTTTGTTGACTTCAATGAAGGGGAAATCAAGTTCATCCCCACTTATAAGTATGAC
  TCTAAAACAGACCGGTGGGATTCCAGTGGGAAATGCCGGGTTCCAGCCTGGTGTGACCGA
  ATTCTTTGGAGAGGAACAAATGTTAATCAGCTTAATTATCGGAGTCACATGGAACTGAAA
  ACCAGCGACCACAAGCCTGTTAGCGCCCTCTTCCATATTGGGGTGAAGGTTGTGGATGAA
  CGAAGGTACCGGAAAGTCTTTGAAGATAGTGTACGCATCATGGACAGAATGGAAAATGAC
  TTCCTTCCTTCCTTAGAACTCAGCAGGAGGGAGTTTGTGTTTGAAAATGTGAAGTTTCGG
  CAACTACAAAAGGAGAAGTTCCAGATCAGCAACAATGGACAGGTTCCCTGCCATTTTTCT
  TTCATCCCTAAACTTAATGACAGCCAGTACTGCAAGCCATGGCTTCGGGCTGAACCTTTT
  GAGGGCTACTTGGAGCCAAATGAGACAGTGGACATTTCTCTTGATGTGTATGTCAGCAAA
  GACTCTGTAACCATCCTGAACTCGGGAGAAGATAAGATTGAAGATATTCTCGTCCTTCAC
  CTGGATCGAGGCAAAGATTACTTCTTGACTATCAGTGGAAATTACCTCCCAAGTTGTTTT
  GGCACATCCTTAGAGGCTCTGTGCCGTATGAAAAGACCAATCCGAGAAGTTCCTGTTACC
  AAACTCATAGACTTGGAGAAATCCCTTCTGCAAATGGTTCCTTTGGATGAAGGTGCCAGT
  GAGAGACCCCTTCAGGTTCCCAAGGAGATCTGGCTTCTAGTAGATCACCTATTCAAATAC
  GCCTGTCACCAGGAGGACCTGTTCCAGACCCCTGGAATGCAGGAAGAGCTCCAGCAGATC
  ATTGATTGTCTGGATACCAGCATTCCTGAGACAATCCCTGGCAGCAACCACTCTGTGGCT
  GAAGCACTGCTCATTTTCTTGGAAGCCCTGCCAGAGCCAGTCATCTGTTACGAGCTGTAT
  CAGCGATGTCTTGACTCTGCTTATGATCCCCGGATCTGCCGACAGGTGATCTCCCAGCTT
  CCGAGATGCCATAGAAATGTTTTCCGTTACTTGATGGCATTCCTTCGAGAACTCTTAAAA
  TTCTCTGAATACAATAGCGTCAATGCCAACATGATCGCTACTCTCTTCACTAGTCTTCTC
  CTGAGGCCTCCACCCAACCTTATGGCAAGACAGACTCCAAGTGACCGCCAGCGTGCTATT
  CAGTTCCTTCTGGGCTTTCTGCTTGGGAGCGAAGAAGACTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001587 unedited
  CATTATGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCGCAGCCGAGGT
  GGGTGGGTGTGGGGACGCGGGAGCCAGTGTCGTCGGATCGGCCCGCAGTCCGCTGTCCTG
  CTGAGCCCGGAGGCCGCCTGGATGGAGCCGCCGCTCCCGGTCGGAGCCCAGCCGCTTGCC
  ACTGTCGAGGGTATGGAGATGAAGGGTCCTCTCCGGGAGCCCTGCGCCCTGACCCTAGCC
  CAGAGGAACGGGCAATATGAGTTAATAATCCAGTTGCATGAGAAGGAACAGCATGTTCAA
  GATATCATTCCTATAAATAGCCACTTCAGATGTGTTCAAGAAGCAGAAGAAACTCTTTTG
  ATTGACATAGCTTCTAACAGTGGCTGCAAAATTCGGGTTCAGGGGGACTGGATCAGAGAG
  CGCCGCTTTGAAATCCCTGATGAGGAACACTGTTTGAAGTTCCTCTCAGCTGTCCTTGCT
  GCTCAGAAAGCTCAGTCACAGCTTCTTGTTCCAGAGCAAAAGGACTCATCTAGCTGGTAC
  CAGAAATTAGACACTAAGGACAAACCTTCTGTTTTTTCAGGGCTTCTTGGATTTGAAGAC
  AATTTTTCTTCTATGAATTTGGACAAGAAAATAAATTCACAAAATCAGCCTACTGGGATT
  CATCGGGAACCCCCACCTCCACCCTTTTCAGTGAATAAAATGCTTCCACGTGAAAAAGAA
  GCTTCTAACAAGGAGCAGCCCAAAGTGACCAACACCATGCGGAAGCTCTTTGTACCAAAT
  ACCCAATCTGGGCAGCGGGAGGGTCTCATCAAACATATCCTGGCANAGCGAGAGAAAGAA
  TATGTCAACATTCAGAACTTCAGATTTTNTGTTGGAACTTGGNATGTGAATGCCCAGTCT
  CCAGATAGCGGGNTAGAACCTTGCNCTGACTGTGATCCCCATCCTNCTGATATCTACTGC
  ATNGGATTCCAGAACTGGNACTGAGCACAAAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001587 unedited
  TTCNTTGNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTAACAC
  AACATATATTTATTCAGAAAAAATTTGCAATAAATTATAATAAAAAAGAGACACGAAATA
  TAGAATCCCACTAGCAGCTTTGCTTAGTGATCAAGACGTTTTTGGGAAATATAACAAACA
  ATTACAGAATAAGAAAACAGTGAAAGGGAGACTAATGCAATGTCACCACATGGATTTGAA
  CCATTTCTTCCCTAAGACAGCAGGTGCAGAGTCTTTCCCTACCAGCTGAATCCGAAATCC
  TTACTTCAGAGCAGCTACAAATTAGTACCTTAAAGGACTTGGCAATTACAGGTTGGGGTG
  CTGGAATAGCCATGGTAGGGAAGCAGATTTCTTCTGTCTGGGTCTACATCTTGTCTCCTC
  TACCTAGAGATTGTCTAAAACTCTCAACTCTGTATATGTTCCAGGATGAGCTGAAAAAAG
  GTCGAGGCAGTACACCAACTTAGTGTTTTGATCTGATGTACAAAGAGATAGGATACTGCA
  TACGTGCAGCACAGAGCCTCCACTAACCACACCCTTGATCACCCAAAGATCCCAGGTCAG
  CGATATGCTAGAAAACAAGCCCAGGAAAGGTATTTTCCTGTGGAATGACCCTACCCAGTG
  CCTATCATCTGCCTCTTTTTGAGATCACTTTTTGGCCCTGCGGAGATGAACAACTGCTCT
  TGCCTAAGTCTTCCATTAACTAAGTCCAACACTACCCCCCGACTTATATACCTGCAAATC
  TGGGCCCACTCCTGGACTTTAAGCTTTACTTCTAAGAGCACGGGTGATCCCTCTGCCACA
  TACCCCCTTGCCCCCCCCTCCCGATCACATTGACTTCCCTGGTGCCCCTCCCCTCCTCAA
  TGCCCACACGGTCCCCCGATTACTTACGTCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001587
• Insert Size: 5120 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001587.3, NP_001578.2
• RefSeq Size: 5141 bp
• RefSeq ORF: 2682 bp
• Locus ID: 4952
• Cytogenetics: Xq26.1
• Domains: RhoGAP, IPPc, Exo_endo_phos
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: 'This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (b) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b) that has a shorter C-terminus, compared to isoform 1.