alpha smooth muscle Actin (ACTA2) (NM_001613) Human Untagged Clone

CAT#: SC119152

ACTA2 (untagged)-Human actin, alpha 2, smooth muscle, aorta (ACTA2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACTA2
• Synonyms: ACTSA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119152 sequence for NM_001613 edited (data generated by NextGen Sequencing)
  ATGTGTGAAGAAGAGGACAGCACTGCCTTGGTGTGTGACAATGGCTCTGGGCTCTGTAAG
  GCCGGCTTTGCTGGGGACGATGCTCCCAGGGCTGTTTTCCCATCCATTGTGGGACGTCCC
  AGACATCAGGGGGTGATGGTGGGAATGGGACAAAAAGACAGCTACGTGGGTGACGAAGCA
  CAGAGCAAAAGAGGAATCCTGACCCTGAAGTACCCGATAGAACATGGCATCATCACCAAC
  TGGGACGACATGGAAAAGATCTGGCACCACTCTTTCTACAATGAGCTTCGTGTTGCCCCT
  GAAGAGCATCCCACCCTGCTCACGGAGGCACCCCTGAACCCCAAGGCCAACCGGGAGAAA
  ATGACTCAAATTATGTTTGAGACTTTCAATGTCCCAGCCATGTATGTGGCTATCCAGGCG
  GTGCTGTCTCTCTATGCCTCTGGACGCACAACTGGCATCGTGCTGGACTCTGGAGATGGT
  GTCACCCACAATGTCCCCATCTATGAGGGCTATGCCTTGCCCCATGCCATCATGCGTCTG
  GATCTGGCTGGCCGAGATCTCACTGACTACCTCATGAAGATCCTGACTGAGCGTGGCTAT
  TCCTTCGTTACTACTGCTGAGCGTGAGATTGTCCGGGACATCAAGGAGAAACTGTGTTAT
  GTAGCTCTGGACTTTGAAAATGAGATGGCCACTGCCGCATCCTCATCCTCCCTTGAGAAG
  AGTTACGAGTTGCCTGATGGGCAAGTGATCACCATCGGAAATGAACGTTTCCGCTGCCCA
  GAGACCCTGTTCCAGCCATCCTTCATCGGGATGGAGTCTGCTGGCATCCATGAAACCACC
  TACAACAGCATCATGAAGTGTGATATTGACATCAGGAAGGACCTCTATGCTAACAATGTC
  CTATCAGGGGGCACCACTATGTACCCTGGCATTGCCGACCGAATGCAGAAGGAGATCACG
  GCCCTAGCACCCAGCACCATGAAGATCAAGATCATTGCCCCTCCGGAGCGCAAATACTCT
  GTCTGGATCGGTGGCTCCATCCTGGCCTCTCTGTCCACCTTCCAGCAGATGTGGATCAGC
  AAACAGGAATACGATGAAGCCGGGCCTTCCATTGTCCACCGCAAATGCTTCTAA
  
  Clone variation with respect to NM_001613.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001613 unedited
  NGGTCGAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGAGCAGCCC
  AGCCAAGCACTGTCAGGAATCCTGTGAAGCAGCTCCAGCTATGTGTGAAGAAGAGGACAG
  CACTGCCTTGGTGTGTGACAATGGCTCTGGGCTCTGTAAGGCCGGCTTTGCTGGGGACGA
  TGCTCCCAGGGCTGTTTTCCCATCCATTGTGGGACGTCCCAGACATCAGGGGGTGATGGT
  GGGAATGGGACAAAAAGACAGCTACGTGGGTGACGAAGCACAGAGCAAAAGAGGAATCCT
  GACCCTGAAGTACCCGATAGAACATGGCATCATCACCAACTGGGACGACATGGAAAAGAT
  CTGGCACCACTCTTTCTACAATGAGCTTCGTGTTGCCCCTGAAGAGCATCCCACCCTGCT
  CACGGAGGCACCCCTGAACCCCAAGGCCAACCGGGAGAAAATGACTCAAATTATGTTTGA
  GACTTTCAATGTCCCAGCCATGTATGTGGCTATCCAGGCGGTGCTGTCTCTCTATGCCTC
  TGGACGCACAACTGGCATCGTGCTGGACTCTGGAGATGGTGTCACCCACAATGTCCCCAT
  CTATGAGGGCTATGCCTTGCCCCATGCCATCATGCGTCTGGATCTGGCTGGCCGAGATCT
  CACTGACTACCTCATGAAGATCCTGACTGAGCGTGGCTATTCCTTCGTTACTACTGCTGA
  GCGTGAGATTGTCCGGGACATCAAGGAGAAACTGTGNTATGTAGCTCTGGACTNTGAAAA
  TGAGATGGCCACTGCCGCATCCTCATCCTCCCTTGAGAAGAGTTACGAGTTGCCTGATGG
  GCAAGTGATCACCATCGAANATGAACGTTTTCGCTGCCCANANACCCCTGTCCAGCCATC
  CTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001613 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCAGTAGCCTATTTCA
  GATTTATTAAAAAACACATAGGTAACGAGTCAGAGCTTTGGCTAGGAATGATTTGGAAAA
  GAACTGAAGGCATAATTCCACAGGACATTCACAGTTGTGTGCTAGAGACAGAGAGGAGCA
  GGAAAGTGTTTTAGAAGCATTTGCGGTGGACAATGGAAGGCCCGGCTTCATCGTATTCCT
  GTTTGCTGATCCACATCTGCTGGAAGGTGGACAGAGAGGCCAGGATGGAGCCACCGATCC
  AGACAGAGTATTTGCGCTCCGGAGGGGCAATGATCTTGATCTTCATGGTGCTGGGTGCTA
  GGGCCGTGATCTCCTTCTGCATTCGGTCGGCAATGCCAGGGTACATAGTGGTGCCCCCTG
  ATAGGACATTGTTAGCATAGAGGTCCTTCCTGATGTCAATATCACACTTCATGATGCTGT
  TGTAGGTGGTTTCATGGATGCCAGCAGACTCCATCCCGATGAAGGATGGCTGGAACAGGG
  TCTCTGGGCAGCGGAAACGTTCATTTCCGATGGTGATCACTTGCCCATCAGGCAACTCGT
  AACTCTTCTCAAGGGAGGATGAGGATGCGGCAGTGGCCATCTCATTTTCAAAGTCCAGAG
  CTACATAACACAGTTTCTCCTTGATGTCCCGGACAATCTCACGCTCAGCAGTAGTTACGA
  AGGAATAGCCACGCTCAGTCAGGATCTTCATGANGGTAGTCAGTGAGATCTCGGCCAGCC
  AGATCCAGACGCCTGATGGCATGGGGCAAAGGCTTACCCTCATAGATGGGGACATTGGGG
  GGACACCTTTCCAAAGTCACACCGAGCCCGTTGGGCGCTCAAAGCATAAAGAGACACACC
  CGCTGGAAGCCCCCTACCTGGCTGGGACTTGGAAAGTTCAAACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001613
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001613.1, NP_001604.1
• RefSeq Size: 1330 bp
• RefSeq ORF: 1134 bp
• Locus ID: 59
• Cytogenetics: 10q23.31
• Domains: ACTIN
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: 'This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-3 encode the same protein.