GTF2H3 (NM_001516) Human Untagged Clone

CAT#: SC119181

GTF2H3 (untagged)-Human general transcription factor IIH, polypeptide 3, 34kDa (GTF2H3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF2H3
• Synonyms: BTF2; P34; TFB4; TFIIH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119181 sequence for NM_001516 edited (data generated by NextGen Sequencing)
  ATGGTTTCAGACGAAGATGAATTGAATCTTCTGGTTATTGTAGTTGATGCCAACCCAATT
  TGGTGGGGAAAGCAAGCATTAAAGGAATCTCAGTTCACTTTATCCAAATGCATAGATGCC
  GTGATGGTGCTGGGAAATTCGCATTTATTCATGAATCGTTCCAACAAACTTGCTGTGATA
  GCAAGTCACATTCAAGAAAGCCGATTCTTATATCCTGGAAAGAATGGCAGACTTGGAGAC
  TTCTTCGGAGACCCTGGCAACCCTCCTGAATTTAATCCCTCTGGGAGTAAAGATGGAAAA
  TACGAACTTTTAACCTCAGCAAATGAAGTTATTGTTGAAGAGATTAAAGATCTAATGACC
  AAAAGTGACATAAAGGGTCAACATACAGAAACTTTGCTGGCAGGATCCCTGGCCAAAGCC
  CTTTGCTACATTCATAGAATGAACAAGGAAGTTAAAGACAATCAGGAAATGAAATCAAGG
  ATATTGGTGATTAAGGCTGCAGAAGACAGTGCGTTGCAGTATATGAACTTCATGAATGTC
  ATCTTTGCAGCACAGAAACAGAATATTTTGATTGATGCCTGTGTTTTAGACTCCGACTCA
  GGGCTCCTCCAACAGGCTTGTGACATCACGGGAGGACTGTACCTGAAGGTGCCTCAGATG
  CCTTCTCTTCTGCAGTATTTGCTGTGGGTGTTTCTTCCCGATCAAGATCAGAGATCTCAG
  TTAATCCTCCCACCCCCGGTTCATGTTGACTACAGGGCTGCTTGCTTCTGTCATCGAAAT
  CTCATTGAAATTGGTTATGTCTGTTCTGTGTGTTTGTCAATATTCTGCAATTTCAGCCCC
  ATTTGTACTACGTGCGAGACAGCCTTTAAAATTTCTCTGCCTCCAGTGCTGAAAGCCAAG
  AAAAAGAAACTGAAAGTGTCTGCCTGA
  
  Clone variation with respect to NM_001516.3
  738 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001516 unedited
  NGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGGACAGC
  CATGGTTTCAGACGAAGATGAATTGAATCTTCTGGTTATTGTAGTTGATGCCAACCCAAT
  TTGGTGGGGAAAGCAAGCATTAAAGGAATCTCAGTTCACTTTATCCAAATGCATAGATGC
  CGTGATGGTGCTGGGAAATTCGCATTTATTCATGAATCGTTCCAACAAACTTGCTGTGAT
  AGCAAGTCACATTCAAGAAAGCCGATTCTTATATCCTGGAAAGAATGGCAGACTTGGAGA
  CTTCTTCGGAGACCCTGGCAACCCTCCTGAATTTAATCCCTCTGGGAGTAAAGATGGAAA
  ATACGAACTTTTAACCTCAGCAAATGAAGTTATTGTTGAAGAGATTAAAGATCTAATGAC
  CAAAAGTGACATAAAGGGTCAACATACAGAAACTTTGCTGGCAGGATCCCTGGCCAAAGC
  CCTTTGCTACATTCATAGAATGAACAAGGAAGTTAAAGACAATCAGGAAATGAAATCAAG
  GATATTGGTGATTAAGGCTGCAGAAGACAGTGCGTTGCAGTATATGAACTTCATGAATGT
  CATCTTTGCAGCACAGAAACAGAATATTTTGATTGATGCCTGTGTTTTAGACTCCGACTC
  AGGGCTCCTCCAACAGGCTTGTGACATCACGGGAGGACTGTACCTGAAGGTGCCTCAGAT
  GCCTTCTCTTCTGCAGTATTTGCTGTGGGTGTTTCTTCCCGATCAAGATCAGAGATCTCA
  GTTAATCCTCCCACCCCCGNTTCATGTTGACTACAGGGCTGCTTGCTTCTGTCATCGAAA
  ACTCATTGAAATGGCTATGTCTGGTCTGTGTGGTTGGCAATATTCTGCAATTCCAGCCCC
  ATTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001516
• Insert Size: 4290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001516.3, NP_001507.2
• RefSeq Size: 1505 bp
• RefSeq ORF: 927 bp
• Locus ID: 2967
• Cytogenetics: 12q24.31
• Domains: Tfb4
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors, Nucleotide excision repair
• Gene Summary: 'This gene encodes a member of the TFB4 family. The encoded protein is a subunit of the core-TFIIH basal transcription factor and localizes to the nucleus. The encoded protein is involved in RNA transcription by RNA polymerase II and nucleotide excision repair and associates with the Cdk-activating kinase complex. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 14. [provided by RefSeq, Dec 2012]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).