Hyaluronan synthase 1 (HAS1) (NM_001523) Human Untagged Clone

CAT#: SC119184

HAS1 (untagged)-Human hyaluronan synthase 1 (HAS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HAS1
• Synonyms: HAS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001523, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGACAGCAGGACGCGCCCAAGCCCACTCCTGCAGCCTGCCGCTGCTCCGGCCTGGCCCGGAGGGTGC
  TGACCATCGCCTTCGCCCTGCTCATCCTGGGCCTCATGACCTGGGCCTACGCCGCCGGGGTGCCGCTGGC
  CTCCGATCGCTACGGCCTCCTGGCCTTCGGCCTCTACGGGGCCTTCCTTTCAGCGCACCTGGTGGCGCAG
  AGCCTCTTCGCGTACCTGGAGCACCGGCGGGTGGCGGCGGCGGCGCGGGGGCCGCTGGATGCAGCCACCG
  CGCGCAGTGTGGCGCTGACCATCTCCGCCTACCAGGAGGACCCCGCGTACCTGCGCCAGTGCCTGGCGTC
  CGCCCGCGCCCTGCTGTACCCGCGCGCGCGGCTGCGCGTCCTCATGGTGGTGGATGGCAACCGCGCCGAG
  GACCTCTACATGGTCGACATGTTCCGCGAGGTCTTCGCTGACGAGGACCCCGCCACGTACGTGTGGGACG
  GCAACTACCACCAGCCCTGGGAACCCGCGGCGGCGGGCGCGGTGGGCGCCGGAGCCTATCGGGAGGTGGA
  GGCGGAGGATCCTGGGCGGCTGGCAGTGGAGGCGCTGGTGAGGACTCGCAGGTGCGTGTGCGTGGCGCAG
  CGCTGGGGCGGCAAGCGCGAGGTCATGTACACAGCCTTCAAGGCGCTCGGAGATTCGGTGGACTACGTGC
  AGGTCTGTGACTCGGACACAAGGTTGGACCCCATGGCACTGCTGGAGCTCGTGCGGGTACTGGACGAGGA
  CCCCCGGGTAGGGGCTGTTGGTGGGGACGTGCGGATCCTTAACCCTCTGGACTCCTGGGTCAGCTTCCTA
  AGCAGCCTGCGATACTGGGTAGCCTTCAATGTGGAGCGGGCTTGTCAGAGCTACTTCCACTGTGTATCCT
  GCATCAGCGGTCCTCTAGGCCTATATAGGAATAACCTCTTGCAGCAGTTTCTTGAGGCCTGGTACAACCA
  GAAGTTCCTGGGTACCCACTGTACTTTTGGGGATGACCGGCACCTCACCAACCGCATGCTCAGCATGGGT
  TATGCTACCAAGTACACCTCCAGGTCCCGCTGCTACTCAGAGACGCCCTCGTCCTTCCTGCGGTGGCTGA
  GCCAGCAGACACGCTGGTCCAAGTCGTACTTCCGTGAGTGGCTGTACAACGCGCTCTGGTGGCACCGGCA
  CCATGCGTGGATGACCTACGAGGCGGTGGTCTCCGGCCTGTTCCCCTTCTTCGTGGCGGCCACTGTGCTG
  CGTCTGTTCTACGCGGGCCGCCCTTGGGCGCTGCTGTGGGTGCTGCTGTGCGTGCAGGGCGTGGCACTGG
  CCAAGGCGGCCTTCGCGGCCTGGCTGCGGGGCTGCCTGCGCATGGTGCTTCTGTCGCTCTACGCGCCCCT
  CTACATGTGTGGCCTCCTGCCTGCCAAGTTCCTGGCGCTAGTCACCATGAACCAGAGTGGCTGGGGCACC
  TCGGGCCGGCGGAAGCTGGCCGCTAACTACGTCCCTCTGCTGCCCCTGGCGCTCTGGGCGCTGCTGCTGC
  TTGGGGGCCTGGTCCGCAGCGTAGCACACGAGGCCAGGGCCGACTGGAGCGGCCCTTCCCGCGCAGCCGA
  GGCCTACCACTTGGCCGCGGGGGCCGGCGCCTACGTGGGCTACTGGGTGGCCATGTTGACGCTGTACTGG
  GTGGGCGTGCGGAGGCTTTGCCGGCGGCGGACCGGGGGCTACCGCGTCCAGGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001523 unedited
  NGGTTCACAATTTGTAAACGACTCATATAGGCGGCCGCGNATTCCCGGGATAGCAAGCCG
  GAGAGAAGAGAGAGCCCGGCCAGACCCACTGCGATGAGACAGGACGCGCCCAAGCCCACT
  CCTGCAGCCCGCCGCTGCTCCGGCCTGGCCCGGAGGGTGCTGACCATCGCCTTCGCCCTG
  CTCATCCTGGGCCTCATGACCTGGGCCTACGCCGCCGGGGTGCCGCTGGCCTCCGATCGC
  TACGGCCTCCTGGCCTTCGGCCTCTACGGGGCCTTCCTTTCAGCGCACCTGGTGGCGCAG
  AGCCTCTTCGCGTACCTGGAGCACCGGCGGGTGGCGGCGGCGGCGCGGGGGCCGCTGGAT
  GCAGCCACCGCGCGCAGTGTGGCGCTGACCATCTCCGCCTACCAGGAGGACCCCGCGTAC
  CTGCGCCAGTGCCTGGCGTCCGCCCGCGCCCTGCTGTACCCGCGCGCGCGGCTGCGCGTC
  CTCATGGTGGTGGATGGCAACCGCGCCGAGGACCTCTACATGGTCGACATGTTCCGCGAG
  GTCTTCGCTGACGAGGACCCCGCCACGTACGTGTGGGACGGCAACTACCACCAGCCCTGG
  GAACCCGCGGCGGCGGGCGCGGTGGGCGCCGGAGCCTATCGGGAGGTGGACGCGGAGGAT
  CCTGGGCGGCTGGCAGTGGAGGCGCTGGTGAGGACTCGTANGTGCGTGTGCGTGGCGCAN
  CGCTGGGGCGGCAAGCGCGAGGTCATGTACACAGCCTTCAAGGCGCTCGGAGATTCGATG
  GACTACGTGCAGGTCTGTGACTCGGACACAAGGTTGGACCCCATGGCACTGCTGNAGCTC
  GTGCGGGTACTGGNACGAGACCCCCGGNTAGGNNGCTGTTGTGGGGACGTGCGGATCCCT
  TACCCTCTGNACTCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001523 unedited
  TTTTTTTTTTTTTTTTTGAAAATAAATAAAATTCTTTATTACATCCTGATCACACAGTAG
  AAATGGAGATTAAATAAGAACGAGGAGAAAGCAGGACCCTTTCCCTCCACTCCTCAGATC
  CCACACCCTGACCAATAAATACCCTCCCTGGAGACACCNAGNNNNNAAANNNAAGNNNNN
  NNNTTTNNNN
  
• Restriction Sites: Please inquire
• ACCN: NM_001523
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001523.1, NP_001514.1
• RefSeq Size: 2088 bp
• RefSeq ORF: 1737 bp
• Locus ID: 3036
• Cytogenetics: 19q13.41
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase (DG42) from Xenopus laevis, and a recently described murine hyaluronan synthase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).