HIF-1 alpha (HIF1A) (NM_001530) Human Untagged Clone

CAT#: SC119189

HIF1A (untagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIF1A
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001530 edited
  GAATTCGGCACGAGGCCTCTTCGTCGCTTCGGCCAGTGTGTCGGGCTGGGCCCTGACAAG
  CCACCTGAGGAGAGGCTCGGAGCCGGGCCCGGACCCCGGCGATTGCCGCCCGCTTCTCTC
  TAGTCTCACGAGGGGTTTCCCGCCTCGCACCCCCACCTCTGGACTTGCCTTTCCTTCTCT
  TCTCCGCGTGTGGAGGGAGCCAGCGCTTAGGCCGGAGCGAGCCTGGGGGCCGCCCGCCGT
  GAAGACATCGCGGGGACCGATTCACCATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAA
  AGATAAGTTCTGAACGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTA
  AAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCACATAATGTGAGTT
  CGCATCTTGATAAGGCCTCTGTGATGAGGCTTACCATCAGCTATTTGCGTGTGAGGAAAC
  TTCTGGATGCTGGTGATTTGGATATTGAAGATGACATGAAAGCACAGATGAATTGCTTTT
  ATTTGAAAGCCTTGGATGGTTTTGTTATGGTTCTCACAGATGATGGTGACATGATTTACA
  TTTCTGATAATGTGAACAAATACATGGGATTAACTCAGTTTGAACTAACTGGACACAGTG
  TGTTTGATTTTACTCATCCATGTGACCATGAGGAAATGAGAGAAATGCTTACACACAGAA
  ATGGCCTTGTGAAAAAGGGTAAAGAACAAAACACACAGCGAAGCTTTTTTCTCAGAATGA
  AGTGTACCCTAACTAGCCGAGGAAGAACTATGAACATAAAGTCTGCAACATGGAAGGTAT
  TGCACTGCACAGGCCACATTCACGTATATGATACCAACAGTAACCAACCTCAGTGTGGGT
  ATAAGAAACCACCTATGACCTGCTTGGTGCTGATTTGTGAACCCATTCCTCACCCATCAA
  ATATTGAAATTCCTTTAGATAGCAAGACTTTCCTCAGTCGACACAGCCTGGATATGAAAT
  TTTCTTATTGTGATGAAAGAATTACCGAATTGATGGGATATGAGCCAGAAGAACTTTTAG
  GCCGCTCAATTTATGAATATTATCATGCTTTGGACTCTGATCATCTGACCAAAACTCATC
  ATGATATGTTTACTAAAGGACAAGTCACCACAGGACAGTACAGGATGCTTGCCAAAAGAG
  GTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAACACCAAGAATTCTCAAC
  CACAGTGCATTGTATGTGTGAATTACGTTGTGAGTGGTATTATTCAGCACGACTTGATTT
  TCTCCCTTCAACAAACAGAATGTGTCCTTAAACCGGTTGAATCTTCAGATATGAAAATGA
  CTCAGCTATTCACCAAAGTTGAATCAGAAGATACAAGTAGCCTCTTTGACAAACTTAAGA
  AGGAACCTGATGCTTTAACTTTGCTGGCCCCAGCCGCTGGAGACACAATCATATCTTTAG
  ATTTTGGCAGCAACGACACAGAAACTGATGACCAGCAACTTGAGGAAGTACCATTATATA
  ATGATGTAATGCTCCCCTCACCCAACGAAAAATTACAGAATATAAATTTGGCAATGTCTC
  CATTACCCACCGCTGAAACGCCAAAGCCACTTCGAAGTAGTGCTGACCCTGCACTCAATC
  AAGAAGTTGCATTAAAATTAGAACCAAATCCAGAGTCACTGGAACTTTCTTTTACCATGC
  CCCAGATTCAGGATCAGACACCTAGTCCTTCCGATGGAAGCACTAGACAAAGTTCACCTG
  AGCCTAATAGTCCCAGTGAATATTGTTTTTATGTGGATAGTGATATGGTCAATGAATTCA
  AGTTGGAATTGGTAGAAAAACTTTTTGCTGAAGACACAGAAGCAAAGAACCCATTTTCTA
  CTCAGGACACAGATTTAGACTTGGAGATGTTAGCTCCCTATATCCCAATGGATGATGACT
  TCCAGTTACGTTCCTTCGATCAGTTGTCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAA
  GCGCAAGTCCTCAAAGCACAGTTACAGTATTCCAGCAGACTCAAATACAAGAACCTACTG
  CTAATGCCACCACTACCACTGCCACCACTGATGAATTAAAAACAGTGACAAAAGACCGTA
  TGGAAGACATTAAAATATTGATTGCATCTCCATCTCCTACCCACATACATAAAGAAACTA
  CTAGTGCCACATCATCACCATATAGAGATACTCAAAGTCGGACAGCCTCACCAAACAGAG
  CAGGAAAAGGAGTCATAGAACAGACAGAAAAATCTCATCCAAGAAGCCCTAACGTGTTAT
  CTGTCGCTTTGAGTCAAAGAACTACAGTTCCTGAGGAAGAACTAAATCCAAAGATACTAG
  CTTTGCAGAATGCTCAGAGAAAGCGAAAAATGGAACATGATGGTTCACTTTTTCAAGCAG
  TAGGAATTGGAACATTATTACAGCAGCCAGACGATCATGCAGCTACTACATCACTTTCTT
  GGAAACGTGTAAAAGGATGCAAATCTAGTGAACAGAATGGAATGGAGCAAAAGACAATTA
  TTTTAATACCCTCTGATTTAGCATGTAGACTGCTGGGGCAATCAATGGATGAAAGTGGAT
  TACCACAGCTGACCAGTTATGATTGTGAAGTTAATGCTCCTATACAAGGCAGCAGAAACC
  TACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCTTTTTCTTAAT
  TTCATTCCTTTTTTTGGACACTGGTGGCTCACTACCTAAAGCAGTCTATTTATATTTTCT
  ACATCTAATTTTAGAAGCCTGGCTACAATACTGCACAAACTTGGTTAGTTCAATTTTTGA
  TCCCCTTTCTACTTAATTTACATTAATGCTCTTTTTTAGTATGTTCTTTAATGCTGGATC
  ACAGACAGCTCATTTTCTCAGTTTTTTGGTATTTAAACCATTGCATTGCAGTAGCATCAT
  TTXXXXXXXXXXXXCTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001530 unedited
  AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATCGGCACGAGGCCTCTTCGT
  CGCTTCGGCCAGTGTGTCGGGCTGGGCCCTGACAAGCCACCTGAGGAGAGGCTCGGAGCC
  GGGCCCGGACCCCGGCGATTGCCGCCCGCTTCTCTCTAGTCTCACGAGGGGTTTCCCGCC
  TCGCACCCCCACCTCTGGACTTGCCTTTCCTTCTCTTCTCCGCGTGTGGAGGGAGCCAGC
  GCTTAGGCCGGAGCGAGCCTGGGGGCCGCCCGCCGTGAAGACATCGCGGGGACCGATTCA
  CCATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTCTGAACGTCGAAAAG
  AAAAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGC
  TTGCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAGGCCTCTGTGA
  TGAGGCTTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGTGATTTGGATA
  TTGAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGCCTTGGATGGTTTTG
  TTATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTGAACAAATACA
  TGGGATTAACTCAGTTTGAACTAACTGGACACAGTGGTGTTTGATTTTACTCATCCATGT
  GACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAAAAGGGTANA
  GAACANAACACACAGCGAAGCTTTNTTCTCAGAATGAAGTGTACCCTAACTAGCCCGAGG
  AGAACTATGAACATAAAGTCTGCACCTTGGAAGGTATTGCACTGACAGGCCACATTCACG
  TATATGATACCACAGTACCACCTCCATGTGGGGTATAAGAACCACTAGACCGGCTGGGGC
  GCTGGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001530 unedited
  CGCGGCCCGCATTCTAANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTCCTGGTTTTAAA
  AAAGTTTATTTGATGGAACAAAACAATACAGTTAGTGTTAGATCCAACCACAAAGAGCAA
  AAGGAATGAAAAATTTGTACAATGTACATAGAAAAAAACAAGATATTTACTGTGACAACT
  ATATATTCCTAAAATAATGCTTCTAAATTTTTTCAATTATTGAAATCTACATGGGGAAAA
  GGATGTTAATAGCGACAAAGTGCATAAAATCAAACATTGTATTTTGAGCAAATTAACATA
  CTAGGCAATTTTGCTAAAAATGCATGATTTTTTTTTTCTTGTTTACAGCCTGCTCAAAAT
  ATCTTTATACCAACAGGGTAGGCACAACATTTAGGTTTAATATCAGTTACACAATATTAG
  CATAAACTTTCACAACTACATAGGGTATTGCCCTCTTTTGACCTGGCAAAGTGACTATAC
  AAACATTATATGATTCCTCTGAATTGACAATTTCATCCAAATAAATGCCACATACCTTGT
  ACATATATGCATATCTTCCTATATTATGCAAATGGCTTTACTCTTTAAAAATTAACCATG
  CATGATACAACAATCATTATCCTCCGATTAACACTGTGCTGCGGCAGCGTCAACACTCGT
  GCTGGCCCAAGAAAAACTTTCGAGCTAAAAACTGTTCTATCAACCCATAATATGTCTCAT
  GAGCCACTGTTGGTCTTCTCTAATAAAAACTCTTGTGCAATGGGGGCTACCACACCCTGT
  TGGCAAACGCCCTTATCTACGCACAAGGGGAAAAAAAGGGGGAAAAATTGCCAACGGCCT
  TTATTTTTATCAAAGTATTGGCCTATTAAAAAATATTTGGACTTTTCTAAAATATTCGAA
  AAGGATAACCTCCCTTCCAAAAAAATAAAAAGGGGCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001530
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001530.2, NP_001521.1
• RefSeq Size: 3958 bp
• RefSeq ORF: 2481 bp
• Locus ID: 3091
• Cytogenetics: 14q23.2
• Domains: PAS, HLH, PAC
• Protein Families: Transcription Factors
• Protein Pathways: mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma
• Gene Summary: 'This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.