GCNT2 (NM_001491) Human Untagged Clone

CAT#: SC119201

GCNT2 (untagged)-Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCNT2
• Synonyms: bA360O19.2; bA421M1.1; CCAT; CTRCT13; GCNT2C; GCNT5; IGNT; II; NACGT1; NAGCT1; ULG3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119201 sequence for NM_001491 edited (data generated by NextGen Sequencing)
  ATGCCTTTATCAATGCGTTACCTCTTCATAATTTCTGTCTCTAGTGTAATTATTTTTATC
  GTCTTCTCTGTGTTCAATTTTGGGGGAGATCCAAGCTTCCAAAGGCTAAATATCTCAGAC
  CCTTTGAGGCTGACTCAAGTTTGCACATCTTTTATCAATGGAAAAACACGTTTCCTGTGG
  AAAAACAAACTAATGATCCATGAGAAGTCTTCTTGCAAGGAATACTTGACCCAGAGCCAC
  TACATCACAGCCCCTTTATCTAAGGAAGAAGCTGACTTTCCCTTGGCATATATAATGGTC
  ATCCATCATCACTTTGACACCTTTGCAAGGCTCTTCAGGGCTATTTACATGCCCCAAAAT
  ATCTACTGTGTTCATGTGGATGAAAAAGCAACAACTGAATTTAAAGATGCGGTAGAGCAA
  CTATTAAGCTGCTTCCCAAACGCTTTTCTGGCTTCCAAGATGGAACCCGTTGTCTATGGA
  GGGATCTCCAGGCTCCAGGCTGACCTGAACTGCATCAGAGATCTTTCTGCCTTCGAGGTC
  TCATGGAAGTACGTTATCAACACCTGTGGGCAAGACTTCCCCCTGAAAACCAACAAGGAA
  ATAGTTCAGTATCTGAAAGGATTTAAAGGTAAAAATATCACCCCAGGGGTGCTGCCCCCA
  GCTCATGCAATTGGACGGACTAAATATGTCCACCAAGAGCACCTGGGCAAAGAGCTTTCC
  TATGTGATAAGAACAACAGCGTTGAAACCGCCTCCCCCCCATAATCTCACAATTTACTTT
  GGCTCTGCCTATGTGGCTCTATCAAGAGAGTTTGCCAACTTTGTTCTGCATGACCCACGG
  GCTGTTGATTTGCTCCAGTGGTCCAAGGACACTTTCAGTCCTGATGAGCATTTCTGGGTG
  ACACTCAATAGGATTCCAGGTGTTCCTGGCTCTATGCCAAATGCATCCTGGACTGGAAAC
  CTCAGAGCTATAAAGTGGAGTGACATGGAAGACAGACACGGAGGCTGCCACGGCCACTAT
  GTACATGGTATTTGTATCTATGGAAACGGAGACTTAAAGTGGCTGGTTAATTCACCAAGC
  CTGTTTGCTAACAAGTTTGAGCTTAATACCTACCCCCTTACTGTGGAATGCCTAGAACTG
  AGGCATCGCGAAAGAACCCTCAATCAGAGTGAAACTGCGATACAACCCAGCTGGTATTTT
  TGA
  
  Clone variation with respect to NM_001491.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001491 unedited
  NGTTCAAATATTTGTATACGACTCACTATAGGGCGGCCGCGACATTCGCACGAGTCGGGA
  TGAAACGGAATCGATTCCCAGCGTCTCCAACAGGCAGGAGTGAGTGGAGTATGTTGCAAA
  ATAAGAACTCAGAGAAACGAGTGAGTTTGNGAAAAAAGACTTACAGATTTTGACGGTCTC
  TTGACATTTCACCCTTCTTTGAGGCATGCCTTTATCAATGCGTTACCTCTTCATAATTTC
  TGTCTCTAGTGTAATTATTTTTATCGTCTTCTCTGTGTTCAATTTTGGGGGAGATCCAAG
  CTTCCAAAGGCTAAATATCTCAGACCCTTTGAGGCTGACTCAAGTTTGCACATCTTTTAT
  CAATGGAAAAACACGTTTCCTGTGGAAAAACAAACTAATGATCCATGAGAAGTCTTCTTG
  CAAGGAATACTTGACCCAGAGCCACTACATCACAGCCCCTTTATCTAAGGAAGAAGCTGA
  CTTTCCCTTGGCATATATAATGGTCATCCATCATCACTTTGACACCTTTGCAAGGCTCTT
  CAGGGCTATTTACATGCCCCAAAATATCTACTGTGTTCATGTGGATGAAAAAGCAACAAC
  TGAATTTAAAGATGCGGTAGAGCAACTATTAAGCTGCTTCCCAAACGCTTTTCTGGCTTC
  CAAGATGGAACCCGTTGTCTATGGNANGGATCTCCAGGCTCCAGGCTGACCTGAACTGCA
  TCAGAGATCTTTCTGCCTTCGAAGTCTCATGGAAAGTACGTTATCAACACCTGTGGGCAA
  GACTTNNCCCTGAAACCACAAGNAATAGTTCATATCTGAAAGATTTAAAGTAAAAATACC
  CCAGGGGTGCTGCCCACTCAGCAATGGACGACTAAAATGTNCACAAGACACCTGGCAAGA
  GCTTNCTAGTGATAGACAACACGTGAAAACGCTCCCCCATATCTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001491 unedited
  NNTTTGGTAAATCTAAGNAACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTTTTT
  TTTTGGAAAACAATATATTAATTTATTCCCAAAAAAAAAAACATGGAACCTCATCGAAAA
  CAAACATCAGATACAGTAGTTTCAGGGCATTCCCATGGGCTGTCAGAAACCCACTAGCCC
  AAACACTATGCTCTTCCAACATTAAAGTATTTAAGACACAGGAGAGATGACCAACAACAG
  ACATGTAAACATCAATTCCAGAAAGATTTCTTGTTCTACATGAGAAACATCCAGTGTAAA
  ACGGTTCCTCTTCTCAGGTGCTGTCATTTAAATAAGTTGAAAAGAGACAATTTTTAAACT
  CCACTGCTGACCCTGAGTGCATTCGCTATCCCCTCACCTATTTTGTTTTGGGACAAAGTC
  TCGCTCTGTCACCCAGGCTGGAGTGCAGTGGGGCACTCTCAGCTCACTGTAACCTCCACC
  TCCTGGGTTCAAGCGATTCTCATGCCTCAGCCTGCCAAATAGCTGGGATTACAGGCACAT
  GCCACAAAGCCCGGCTAATTTTTATATTTTTTAGTAAAGATGGGGTTTCACCATGTCGGC
  CAGCCTGGTCTGGAACTCCTGGCATCAAGTGATCTACCTGCCTTGGCCTCCCAAAGTGTT
  GGGATTACAGGTGTGAGCCCACCACGCCCGGCCCAAGCCAGAGGTCTTGTGAGGGGGACT
  AACCCATCCTGAGGGTCCTACCCTCGTGACCTCATCTAAACTTCCCTTACCAAAAGCCCC
  CTCTTCAAAACCAATCACATTGGAGGGGTTAAGGCCCCGACCCTTGGAATTTGGGGGGGA
  CCACAGCCCCTCTATAAGCCCAGGGGCGTTTGTGCTTTGGAGCCCCATCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001491
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001491.2, NP_001482.1
• RefSeq Size: 4691 bp
• RefSeq ORF: 1203 bp
• Locus ID: 2651
• Cytogenetics: 6p24.3-p24.2
• Domains: Branch
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• Gene Summary: 'This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) represents the longest transcript and encodes isoform B. Sequence Note: This RefSeq record represents the GCNT2*001.1.1 allele.