GMDS (NM_001500) Human Untagged Clone

CAT#: SC119207

GMDS (untagged)-Human GDP-mannose 4,6-dehydratase (GMDS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GMDS
• Synonyms: GMD; SDR3E1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119207 sequence for NM_001500 edited (data generated by NextGen Sequencing)
  ATGGCACACGCACCGGCACGCTGCCCCAGCGCCCGGGGCTCCGGGGACGGCGAGATGGGC
  AAGCCCAGGAACGTGGCGCTCATCACCGGTATCACAGGCCAGGATGGTTCCTACCTGGCT
  GAGTTCCTGCTGGAGAAAGGCTATGAGGTCCATGGAATTGTACGGCGGTCCAGTTCATTT
  AATACGGGTCGAATTGAGCATCTGTATAAGAATCCCCAGGCTCACATTGAAGGAAACATG
  AAGTTGCACTATGGCGATCTCACTGACAGTACCTGCCTTGTGAAGATCATTAATGAAGTA
  AAGCCCACAGAGATCTACAACCTTGGAGCCCAGAGCCACGTCAAAATTTCCTTTGACCTC
  GCTGAGTACACTGCGGACGTTGACGGAGTTGGCACTCTACGACTTCTAGATGCAGTTAAG
  ACTTGTGGCCTTATCAACTCTGTGAAGTTCTACCAAGCCTCAACAAGTGAACTTTATGGG
  AAAGTGCAGGAAATACCCCAGAAGGAGACCACCCCTTTCTATCCCCGGTCACCCTATGGG
  GCAGCAAAACTCTATGCCTATTGGATTGTGGTGAACTTCCGTGAGGCGTATAATCTCTTT
  GCAGTGAACGGCATTCTCTTCAATCATGAGAGTCCCAGAAGAGGAGCTAATTTCGTTACT
  CGAAAAATTAGCCGGTCAGTAGCTAAGATTTACCTTGGACAACTGGAATGTTTCAGTTTG
  GGAAATCTGGATGCCAAACGAGATTGGGGCCATGCCAAGGACTATGTGGAGGCTATGTGG
  TTGATGTTGCAGAATGATGAGCCGGAGGACTTCGTTATAGCTACTGGGGAGGTCCATAGT
  GTCCGGGAATTTGTCGAGAAATCATTCTTGCACATTGGAAAAACCATTGTGTGGGAAGGA
  AAGAATGAAAATGAAGTGGGCAGATGTAAAGAGACCGGCAAAGTTCACGTGACTGTGGAT
  CTCAAGTACTACCGGCCAACTGAAGTGGACTTTCTGCAGGGCGACTGCACCAAAGCGAAA
  CAGAAGCTGAACTGGAAGCCCCGGGTCGCTTTCGATGAGCTGGTGAGGGAGATGGTGCAC
  GCCGACGTGGAGCTCATGAGGACAAACCCCAATGCCTGA
  
  Clone variation with respect to NM_001500.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001500 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCGGCCCGCCCC
  CGGCCCTCCCTGCACGGCCTCCCGTGCGCCCCTGTCAGACTGTGGCGGCCGGTCGCGCGG
  TGCGCTCTCCCTCCCTGCCCGCAGCCTGGAGAGGCGCTTCGTGCTGCACACCCCCGCGTT
  CCTGCCGGCACCGCGCCTGCCCTCTGCCGCGCTCCGCCCTGCCGCCGACCGCACGCCCGC
  CGCGGGACATGGCACACGCACCGGCACGCTGCCCCAGCGCCCGGGGCTCCGGGGACGGCG
  AGATGGGCAAGCCCAGGAACGTGGCGCTCATCACCGGTATCACAGGCCAGGATGGTTCCT
  ACCTGGCTGAGTTCCTGCTGGAGAAAGGCTATGAGGTCCATGGAATTGTACGGCGGTCCA
  GTTCATTTAATACGGGTCGAATTGAGCATCTGTATAAGAATCCCCAGGCTCACATTGAAG
  GAAACATGAAGTTGCACTATGGCGATCTCACTGACAGTACCTGCCTTGTGAAGATCATTA
  ATGAAGTAAAGCCCACAGAGATCTACAACCTTGGAGCCCAGAGCCACGTCAAAATTTCCT
  TTGACCTCGCTGAGTACACTGCGGACGTTGACGGAGTTGGCACTCTACGACTTCTAGATG
  CAGTTAAGACTTGTGGCCTTATCAACTCTGTGAAGTTCTACCAAGCCTCCACAAGTGAAC
  TTTATGGGAAAGTGCGGGAATACCCCATAGGAGACCCCCCCTTTCTATTCCCGGTCACCC
  TATGGGGCAGCAAACCTTTATGCCCTATTGGATTGTGGTGAACCTTCCGGGAGGCGGATA
  ATTCNCTTTGCAGGGAAACGGCATTCTCTCTAATCATGAGAGCCCCAACAAGAGAACTAA
  TTTCCGTTACCTCAAAAATTAACCCGGGCGGNAGCTAAGAATTCACCCTGGACACACTGG
  AAGGGTTCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001500 unedited
  CTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCTAGTTCACAGA
  AAGACCATTTTTAATATGAAAAGAGAAAACAATTTGAAGGCCCCACAATTTGTTGTGTAA
  CAACATAATTTCAAGTAAAGTGAATGTGATTAAAACATCTTGATTTCACAAAGCCATTCA
  GAGGAGGCTTCGACAAACGCAAAGCGACCCAAACCCTGGAGGGTCACATCCCGGCTGCTA
  CAAACCTCGGCGGGGCGGCCCCGCTCTTGCGGCCGGGACAGCGCAGCGGCAGCAGGGGCC
  GCAGGGGACCCGCAGATTGGCACGCCGCTCCCCATCCCCGCAGCGCGTCTGCACCGGAGA
  CTCTGCGGGGATTGTAGCCGGAGGGCGGGCCGGGCTCCGAGGCGCTGCTCAGGCATTGGG
  GTTTGTCCTCATGAGCTCCACGTCGGCGTGCACCATCTCCCTCACCAGCTCATCGAAAGC
  GACCCGGGGCTTCCAGTTCAGCTTCTGTTTCGCTTTGGTGCAGTCGCCCTGCAGAAAGTC
  CACTTCAGTTGGCCGGTAGTACTTGAGATCCACAGTCACGTGAACTTTGCCGGTCTCTTT
  ACATCTGCCCACTTCATTTTCATTCTTTCCTTCCCACACAATGGTTTTTCCAATGTGCAA
  GAATGATTTCTCGACAAATTCCCGGACACTATGGACCTNNCCAGTAGCTATAACGAAGTC
  CTCCGGCTCATCATTCTGCAACATCAACCACATAGCCTCCACATAGTCCTTGGCATGGCC
  CCAATCTCGTTTGGCATCCAGATTTCCAAACTGAAACATCCAGTTGTCCAAGGTAAATCT
  AACTACTGACCGGCTAATTTTCGAGTACGAAATTAGCTCCTCTTCTGGGACTCTATGATT
  GAAGAAATGCCGTTACTGCAAGAGATATACCCTCACGGAATCACCACCATCCATGGCATA
  AAGTTTGCTGCCCATAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001500
• Insert Size: 1860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001500.2, NP_001491.1
• RefSeq Size: 1698 bp
• RefSeq ORF: 1119 bp
• Locus ID: 2762
• Cytogenetics: 6p25.3
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: 'GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020).[supplied by OMIM, Aug 2009]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).