GMDS (NM_001500) Human Untagged Clone

CAT#: SC119207

GMDS (untagged)-Human GDP-mannose 4,6-dehydratase (GMDS)


  "NM_001500" in other vectors (5)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GMDS
Synonyms GMD; SDR3E1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119207 sequence for NM_001500 edited (data generated by NextGen Sequencing)
ATGGCACACGCACCGGCACGCTGCCCCAGCGCCCGGGGCTCCGGGGACGGCGAGATGGGC
AAGCCCAGGAACGTGGCGCTCATCACCGGTATCACAGGCCAGGATGGTTCCTACCTGGCT
GAGTTCCTGCTGGAGAAAGGCTATGAGGTCCATGGAATTGTACGGCGGTCCAGTTCATTT
AATACGGGTCGAATTGAGCATCTGTATAAGAATCCCCAGGCTCACATTGAAGGAAACATG
AAGTTGCACTATGGCGATCTCACTGACAGTACCTGCCTTGTGAAGATCATTAATGAAGTA
AAGCCCACAGAGATCTACAACCTTGGAGCCCAGAGCCACGTCAAAATTTCCTTTGACCTC
GCTGAGTACACTGCGGACGTTGACGGAGTTGGCACTCTACGACTTCTAGATGCAGTTAAG
ACTTGTGGCCTTATCAACTCTGTGAAGTTCTACCAAGCCTCAACAAGTGAACTTTATGGG
AAAGTGCAGGAAATACCCCAGAAGGAGACCACCCCTTTCTATCCCCGGTCACCCTATGGG
GCAGCAAAACTCTATGCCTATTGGATTGTGGTGAACTTCCGTGAGGCGTATAATCTCTTT
GCAGTGAACGGCATTCTCTTCAATCATGAGAGTCCCAGAAGAGGAGCTAATTTCGTTACT
CGAAAAATTAGCCGGTCAGTAGCTAAGATTTACCTTGGACAACTGGAATGTTTCAGTTTG
GGAAATCTGGATGCCAAACGAGATTGGGGCCATGCCAAGGACTATGTGGAGGCTATGTGG
TTGATGTTGCAGAATGATGAGCCGGAGGACTTCGTTATAGCTACTGGGGAGGTCCATAGT
GTCCGGGAATTTGTCGAGAAATCATTCTTGCACATTGGAAAAACCATTGTGTGGGAAGGA
AAGAATGAAAATGAAGTGGGCAGATGTAAAGAGACCGGCAAAGTTCACGTGACTGTGGAT
CTCAAGTACTACCGGCCAACTGAAGTGGACTTTCTGCAGGGCGACTGCACCAAAGCGAAA
CAGAAGCTGAACTGGAAGCCCCGGGTCGCTTTCGATGAGCTGGTGAGGGAGATGGTGCAC
GCCGACGTGGAGCTCATGAGGACAAACCCCAATGCCTGA

Clone variation with respect to NM_001500.2
>OriGene 5' read for NM_001500 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCGGCCCGCCCC
CGGCCCTCCCTGCACGGCCTCCCGTGCGCCCCTGTCAGACTGTGGCGGCCGGTCGCGCGG
TGCGCTCTCCCTCCCTGCCCGCAGCCTGGAGAGGCGCTTCGTGCTGCACACCCCCGCGTT
CCTGCCGGCACCGCGCCTGCCCTCTGCCGCGCTCCGCCCTGCCGCCGACCGCACGCCCGC
CGCGGGACATGGCACACGCACCGGCACGCTGCCCCAGCGCCCGGGGCTCCGGGGACGGCG
AGATGGGCAAGCCCAGGAACGTGGCGCTCATCACCGGTATCACAGGCCAGGATGGTTCCT
ACCTGGCTGAGTTCCTGCTGGAGAAAGGCTATGAGGTCCATGGAATTGTACGGCGGTCCA
GTTCATTTAATACGGGTCGAATTGAGCATCTGTATAAGAATCCCCAGGCTCACATTGAAG
GAAACATGAAGTTGCACTATGGCGATCTCACTGACAGTACCTGCCTTGTGAAGATCATTA
ATGAAGTAAAGCCCACAGAGATCTACAACCTTGGAGCCCAGAGCCACGTCAAAATTTCCT
TTGACCTCGCTGAGTACACTGCGGACGTTGACGGAGTTGGCACTCTACGACTTCTAGATG
CAGTTAAGACTTGTGGCCTTATCAACTCTGTGAAGTTCTACCAAGCCTCCACAAGTGAAC
TTTATGGGAAAGTGCGGGAATACCCCATAGGAGACCCCCCCTTTCTATTCCCGGTCACCC
TATGGGGCAGCAAACCTTTATGCCCTATTGGATTGTGGTGAACCTTCCGGGAGGCGGATA
ATTCNCTTTGCAGGGAAACGGCATTCTCTCTAATCATGAGAGCCCCAACAAGAGAACTAA
TTTCCGTTACCTCAAAAATTAACCCGGGCGGNAGCTAAGAATTCACCCTGGACACACTGG
AAGGGTTCAG
>OriGene 3' read for NM_001500 unedited
CTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCTAGTTCACAGA
AAGACCATTTTTAATATGAAAAGAGAAAACAATTTGAAGGCCCCACAATTTGTTGTGTAA
CAACATAATTTCAAGTAAAGTGAATGTGATTAAAACATCTTGATTTCACAAAGCCATTCA
GAGGAGGCTTCGACAAACGCAAAGCGACCCAAACCCTGGAGGGTCACATCCCGGCTGCTA
CAAACCTCGGCGGGGCGGCCCCGCTCTTGCGGCCGGGACAGCGCAGCGGCAGCAGGGGCC
GCAGGGGACCCGCAGATTGGCACGCCGCTCCCCATCCCCGCAGCGCGTCTGCACCGGAGA
CTCTGCGGGGATTGTAGCCGGAGGGCGGGCCGGGCTCCGAGGCGCTGCTCAGGCATTGGG
GTTTGTCCTCATGAGCTCCACGTCGGCGTGCACCATCTCCCTCACCAGCTCATCGAAAGC
GACCCGGGGCTTCCAGTTCAGCTTCTGTTTCGCTTTGGTGCAGTCGCCCTGCAGAAAGTC
CACTTCAGTTGGCCGGTAGTACTTGAGATCCACAGTCACGTGAACTTTGCCGGTCTCTTT
ACATCTGCCCACTTCATTTTCATTCTTTCCTTCCCACACAATGGTTTTTCCAATGTGCAA
GAATGATTTCTCGACAAATTCCCGGACACTATGGACCTNNCCAGTAGCTATAACGAAGTC
CTCCGGCTCATCATTCTGCAACATCAACCACATAGCCTCCACATAGTCCTTGGCATGGCC
CCAATCTCGTTTGGCATCCAGATTTCCAAACTGAAACATCCAGTTGTCCAAGGTAAATCT
AACTACTGACCGGCTAATTTTCGAGTACGAAATTAGCTCCTCTTCTGGGACTCTATGATT
GAAGAAATGCCGTTACTGCAAGAGATATACCCTCACGGAATCACCACCATCCATGGCATA
AAGTTTGCTGCCCATAG
Restriction Sites NotI-NotI     
ACCN NM_001500
Insert Size 1860 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001500.2, NP_001491.1
RefSeq Size 1698 bp
RefSeq ORF 1119 bp
Locus ID 2762
Cytogenetics 6p25.3
Protein Families Druggable Genome
Protein Pathways Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
Gene Summary 'GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020).[supplied by OMIM, Aug 2009]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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