CXCR3 (NM_001504) Human Untagged Clone

CAT#: SC119210

CXCR3 (untagged)-Human chemokine (C-X-C motif) receptor 3 (CXCR3), transcript variant A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CXCR3
• Synonyms: CD182; CD183; CKR-L2; CMKAR3; GPR9; IP10-R; Mig-R; MigR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001504 edited
  GAATTCGGCACCAGCAGCACACCACCCAGCAGCCAGAGCACCAGCCCAGCCATGGTCCTT
  GAGGTGAGTGACCACCAAGTGCTAAATGACGCCGAGGTTGCCGCCCTCCTGGAGAACTTC
  AGCTCTTCCTATGACTATGGAGAAAACGAGAGTGACTCGTGCTGTACCTCCCCGCCCTGC
  CCACAGGACTTCAGCCTGAACTTCGACCGGGCCTTCCTGCCAGCCCTCTACAGCCTCCTC
  TTTCTGCTGGGGCTGCTGGGCAACGGCGCGGTGGCAGCCGTGCTGCTGAGCCGGCGGACA
  GCCCTGAGCAGCACCGACACCTTCCTGCTCCACCTAGCTGTAGCAGACACGCTGCTGGTG
  CTGACACTGCCGCTCTGGGCAGTGGACGCTGCCGTCCAGTGGGTCTTTGGCTCTGGCCTC
  TGCAAAGTGGCAGGTGCCCTCTTCAACATCAACTTCTACGCAGGAGCCCTCCTGCTGGCC
  TGCATCAGCTTTGACCGCTACCTGAACATAGTTCATGCCACCCAGCTCTACCGCCGGGGG
  CCCCCGGCCCGCGTGACCCTCACCTGCCTGGCTGTCTGGGGGCTCTGCCTGCTTTTCGCC
  CTCCCAGACTTCATCTTCCTGTCGGCCCACCACGACGAGCGCCTCAACGCCACCCACTGC
  CAATACAACTTCCCACAGGTGGGCCGCACGGCTCTGCGGGTGCTGCAGCTGGTGGCTGGC
  TTTCTGCTGCCCCTGCTGGTCATGGCCTACTGCTATGCCCACATCCTGGCCGTGCTGCTG
  GTTTCCAGGGGCCAGCGGCGCCTGCGGGCCATGCGGCTGGTGGTGGTGGTCGTGGTGGCC
  TTTGCCCTCTGCTGGACCCCCTATCACCTGGTGGTGCTGGTGGACATCCTCATGGACCTG
  GGCGCTTTGGCCCGCAACTGTGGCCGAGAAAGCAGGGTAGACGTGGCCAAGTCGGTCACC
  TCAGGCCTGGGCTACATGCACTGCTGCCTCAACCCGCTGCTCTATGCCTTTGTAGGGGTC
  AAGTTCCGGGAGCGGATGTGGATGCTGCTCTTGCGCCTGGGCTGCCCCAACCAGAGAGGG
  CTCCAGAGGCAGCCATCGTCTTCCCGCCGGGATTCATCCTGGTCTGAGACCTCAGAGGCC
  TCCTACTCGGGCTTGTGAGGCCGGAATCCGGGCTCCCCTTTCGCCCACAGTCTGACTTCC
  CCGCATTCCAGGCTCCTCCCTCCCTCTGCCGGCTCTGGCTCTCCCCAATATCCTCGCTCC
  CGGGACTCACTGGCAGCCCCAGCACCACCAGGTCTCCCGGGAAGCCACCCTCCCAGCTCT
  GAGGACTGCACCATTGCTGCTCCTTAGCTGCCAAGCCCCATCCTGCCGCCCGAGGTGGCT
  GCCTGGAGCCCCACTGCCCTTCTCATTTGGAAACTAAAACTTCATCTTCCCCAAGTGCGG
  GGAGTACAAGGCATGGCGTAGAGGGTGCTGCCCCATGAAGCCACAGCCCAGGCCTCCAGC
  TCAGCAGTGACTGTGGCCATGGTCCCCAAGACCTCTATATTTGCTCTTTTATTTTTATGT
  CTAAAATCCTGCTTAAAACTTTTCAATAAACAAGATCGTCAGGAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001504 unedited
  AGGGGCAACCACNNTCCCCCCCCCCCCGTTCAAATTTGTATACGACTCACTATAGGCGGC
  CGCGAATTCGCACCAGCAGCACACCACCCAGCAGCCAGAGCACCAGCCCAGCCATGGTCC
  TTGAGGTGAGTGACCACCAAGTGCTAAATGACGCCGAGGTTGCCGCCCTCCTGGAGAACT
  TCAGCTCTTCCTATGACTATGGAGAAAACGAGAGTGACTCGTGCTGTACCTCCCCGCCCT
  GCCCACAGGACTTCAGCCTGAACTTCGACCGGGCCTTCCTGCCAGCCCTCTACAGCCTCC
  TCTTTCTGCTGGGGCTGCTGGGCAACGGCGCGGTGGCAGCCGTGCTGCTGAGCCGGCGGA
  CAGCCCTGAGCAGCACCGACACCTTCCTGCTCCACCTAGCTGTAGCAGACACGCTGCTGG
  TGCTGACACTGCCGCTCTGGGCAGTGGACGCTGCCGTCCAGTGGGTCTTTGGCTCTGGCC
  TCTGCAAAGTGGCAGGTGCCCTCTTCAACATCAACTTCTACGCAGGAGCCCTCCTGCTGG
  CCTGCATCAGCTNTGACCGCTACCTGAACATAGTTCATGCCACCCAGCTCTACCGCCGGN
  GGCCCCCGGCCCGCGTGACCCTCACCTGCCTGGCTGTCTGGNGGCTCTGCCTGCTTTTCG
  CCCTCCCAGACTTCATCTTCCTGTNCGCCCACCACGACGAGCGCCTCAACGCCACCCACT
  GNCAATACAACTTNCCACAGGTGGGCCGCACGGCTCTGCGGNTGCTGCAACTGGTGCCTT
  GGCTTTCTGCTGCCCCTGCTGGTCATGGCCTACTGCTATGCCCACATCCTGGCCGTGCTG
  CNTGGNTTTCAGGGGCCCAGCGCGCCCTGCGGGCATGCGGCTTGTNGTGGNGGGCCCTGG
  TGGCCTTTGCCTTCTGCTGGNACCCCTTCCACCTGTGGNGCCTGGGGACACTNCTCATGG
  ACCTGGNCGCTTTTGGCCGGCACCGTGGGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001504 unedited
  TACNATGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TCTCCGGACAATCTTGTTTATTGAAAAAGTTTTAACCAGGATTTTAAACCTAAAAATAAA
  AAACCAAATTTAGAGGTCTTGGGGACCATGGCCACAGTCACTGCTGACCTGGAGGCCTGG
  GCTGGGGCTTCATGGGGAAACACCCTTTACCCCATGCCTTGTACTCCCCGCACTTGGGAA
  AAATAAATTTTTATTTTCCAAATGAAAAGGGCAGGGGGGCTCCAGGCAGCCACCTCGGGC
  GGCAGGATGGGGCTTGGCACCTAAGGACCACCAATGGGGCAGCCCTCAAAGCTGGGAGGG
  GGGCTTCCCGGAAAACCTGGGGGGGCTGGGGCTGCCAGTGATTCCCGGGAGCGAGGATTT
  TGGGGAAAGCCAAACCCGGCCAAAGGGAGGGAGGACCCTGGAATGCGGGGAATTCAAACT
  GGGGGCAAAAGGGGACCCCGGATTCCGGCCTCACAACCCCGATTAGGAGGCCTCTGAGGT
  CTCAAACCAGGATGAATCCCGGCGGGAAAACAATGGCTGCCTCTGGACCCCTCTTTGGTT
  GGGGCATCCCAGGCGCAAAATCATATCCAAATTCCGCTCCCGGAACTTGACCCCTACAAA
  GGCATAAACCACCGGGTTGAGGCCCCATTGCTTGTACCCCCAAGCCCGGAGGGGACCCCA
  TTTGGCCAGTTCTACCCTGGGTTTCTTGGCCAAAATTGGGGGGCGAAAACCCCCCAGGCC
  CATGGAGATGTTCCCCCCCCCCCCCCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001504
• Insert Size: 1750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001504.1, NP_001495.1
• RefSeq Size: 1670 bp
• RefSeq ORF: 1107 bp
• Locus ID: 2833
• Cytogenetics: Xq13.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction
• Gene Summary: 'This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1 (also known as CXCR3-A). This isoform functions as a receptor for chemokines CXCL9/Mig, CXCL10/IP-10, and CXCL11/I-TAC (PMID:12782716).