GCKR (NM_001486) Human Untagged Clone

CAT#: SC119244

GCKR (untagged)-Human glucokinase (hexokinase 4) regulator (GCKR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCKR
• Synonyms: FGQTL5; GKRP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119244 sequence for NM_001486 edited (data generated by NextGen Sequencing)
  ATGCCAGGCACAAAACGGTTTCAACATGTCATTGAGACCCCGGAGCCTGGCAAGTGGGAG
  TTGTCTGGGTACGAGGCAGCTGTGCCAATCACGGAGAAGTCAAACCCACTGACCCAGGAT
  CTAGACAAAGCAGATGCTGAGAACATTGTTCGACTGCTAGGGCAATGTGATGCTGAGATC
  TTCCAGGAGGAGGGGCAAGCCCTGTCCACATACCAGAGACTCTACAGCGGATCCATTCTG
  ACCACCATGGTACAGGTGGCTGGGAAAGTTCAGGAAGTGCTGAAGGAGCCAGATGGGGGG
  CTGGTTGTGCTGAGTGGAGGGGGCACCTCTGGCCGGATGGCATTCCTCATGTCGGTGTCC
  TTTAATCAGCTGATGAAAGGTCTGGGACAGAAACCTCTTTACACCTACCTCATTGCAGGT
  GGTGACAGGTCTGTGGTGGCCTCTAGGGAGGGGACAGAAGATAGTGCCTTGCACGGGATT
  GAGGAACTGAAGAAGGTGGCTGCCGGGAAGAAGAGAGTGATTGTCATTGGCATTTCTGTG
  GGACTCTCTGCTCCCTTTGTGGCAGGCCAGATGGACTGCTGCATGAACAACACAGCTGTC
  TTCTTGCCAGTCCTGGTTGGCTTCAATCCAGTGAGCATGGCCAGAAATGACCCCATTGAA
  GACTGGAGTTCAACATTCCGACAAGTAGCAGAGCGGATGCAGAAAATGCAGGAGAAACAG
  AAAGCTTTTGTGCTCAATCCTGCCATCGGGCCCGAGGGTCTCAGCGGCTCCTCCCGGATG
  AAAGGTGGAAGTGCCACCAAGATTCTGCTGGAAACCCTGTTATTAGCAGCCCATAAGACT
  GTGGACCAGGGCATTGCAGCATCTCAAAGATGCCTCCTGGAAATCTTGCGGACATTTGAG
  CGAGCTCATCAGGTGACCTACAGCCAAAGCCCCAAGATTGCCACCCTGATGAAGAGTGTC
  AGCACCAGTCTGGAGAAGAAAGGCCACGTGTACCTGGTTGGCTGGCAGACCCTGGGCATC
  ATTGCCATCATGGATGGAGTAGAGTGCATCCACACCTTTGGTGCTGATTTCCGAGATGTC
  CGTGGCTTTCTCATTGGTGATCACAGTGACATGTTTAACCAGAAGGCTGAGCTCACCAAC
  CAGGGTCCCCAGTTCACCTTCTCCCAGGAGGACTTCCTGACTTCCATCCTTCCCTCTCTC
  ACGGAAATCGATACTGTGGTCTTCATTTTCACCCTGGATGACAACCTCACGGAGGTGCAG
  ACTATAGTGGAGCAGGTGAAAGAGAAGACCAACCACATCCAGGCCCTGGCACACAGCACC
  GTGGGTCAGACCTTGCCGATCCCTCTGAAGAAGCTCTTTCCCTCCATCATCAGCATCACA
  TGGCCACTGCTTTTCTTTGAATATGAAGGGAACTTCATCCAGAAGTTCCAGCGTGAGCTA
  AGCACCAAATGGGTGCTGAATACAGTGAGTACAGGTGCTCATGTGCTTCTTGGTAAGATC
  CTACAAAACCACATGTTGGACCTTCGGATTAGCAACTCCAAGCTCTTCTGGCGGGCGCTG
  GCCATGCTGCAGCGGTTCTCTGGACAGTCCAAGGCTCGATGCATCGAGAGCCTCCTCCGA
  GCGATCCACTTTCCCCAGCCACTGTCAGATGATATTCGGGCTGCTCCCATCTCCTGCCAT
  GTCCAGGTTGCACATGAGAAGGAACAGGTGATACCCATCGCCTTGCTGAGCCTCCTATTC
  CGGTGCTCGATCACTGAGGCTCAGGCACACCTGGCTGCAGCTCCTTCTGTCTGTGAGGCT
  GTCAGGAGTGCTCTTGCTGGGCCAGGTCAGAAGCGCACTGCGGACCCCCTCGAGATCCTA
  GAGCCTGACGTTCAGTGA
  
  Clone variation with respect to NM_001486.3
  230 a=>g;1337 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001486 unedited
  GTTAGCATTTGTATACTATCACTATAGGCGGCACGCGATTCGGCACGATCTGAAGAGCAG
  GAGGAACAGTGTATCCACAGCGTGGGACCATGCCAGGCACAAAACGGTTTCAACATGTCA
  TTGAGACCCCGGAGCCTGGCAAGTGGGAGTTGTCTGGGTACGATGCAGCTGTGCCAATCA
  CGGAGAAGTCAAACCCACTGACCCATGATCTATACAAAGCAGATGCTGATAACATTGTTC
  GACTGCTAAGGCAATGTGATGCTGAGATCTTCCAGGAGGAGGGGCAAGCCCTGTCCACAT
  ACCATAGACTCTACAGCGGATCCATTCTGACCACCATGGTACAGGTGGCTGGGAAAGTTC
  ATGAAGTGCTGAAGGATCCAGATGGGGGGCTGGTTGTGCTGATTGGAGGGGGCACCTCTG
  GCCGGATGGCATTCCTCATGTCGGTGTCCTTTAATCAGCTGATGAAAGGTCTGGGACAGA
  AACCTCTTTACACCTACCTCATTGCAGGTGGTGACAGGTCTGTGGTGGCCTCTATGGAGG
  GGACAGAAGATAGTGCCTTGCACGGGATTGAAGAACTGAAGAATGTGGCTGCCGGGAAGA
  ATATAGTGATTGTCATTGGCATTTCTGTGGGACTCTCTGCTCCCTTTGTGGCAGGCCAGA
  TGGACTGCTGCATGAACAACACAGCTGTCTTCTTGCCAGTCCTGGTTGGCTTCAATCCAG
  TGAGCATGGCCAGAAATGACCCCATTGTAGACTGGAGTTCAACATTCCGACAAGTAGCAT
  AGCGGATGCAGAAAATGCAAGATAAACAGAAAGCTTTTGTGCTCAATCCTGCCATCAGGC
  CCCGATGGTCTCATCGGCTCCTNCCCGATGATATGTGGGAGTGCCCACAAGATTCTGCTG
  GAAAACCTGTTATTATCAGCCATTAGACTGTGCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001486 unedited
  GTTGACCATTGGCTGATGGCAACTTGCCAGGNCCAGTATAGGCCTGGGGNAGGGTCACAG
  GGCATGCCACCCGGGTATCTGTTCAGGCAAACAGCTATGACCGCGGCCGCAATCTAGAGT
  CGAGTTTTTTTTTTTTTTTTTTTTTTTTAACTTTCCAAAATAAGACATTTCATTTTATTT
  CTGAAATCAGAATAAGTCGGTGAGAGTAGAAACCACTAGGTCGAGAGCAAGAACTCTCCC
  CCAAAGTGGAGAGAATATTTCTCCCTACCCTGGGCTGCGGATGCCCTGGAAACGGGGCTT
  CTTCCTCCCACATGTTCTGCTGGCACAAGTCCCCTTGGGCGGGCTGGGCTGAAGTGGGCA
  GGGTTGGGCCCCTTTCACCCACCCAGAAACATGGGTTCACTGAACGTCAGGCTCTAGGAT
  CTCGAGGGGGTCCGCAGTGCGCTTCTGACCTGGCCCAGCAAGAGCACTCCTGACAGCCTC
  ACAGACAGAAGGAGCTGCAGCCAGGTGTGCCTGAGCCTCAGTGATCGAGCACCGGAATAG
  GAGGCTCAGCAAGGCGATGGGTATCACCTGTTCCTTCTCATGTGCAACCTGGACATGGCA
  GGAGATGGGAGCAGCCCGAATATCATCTGACAGTGGCTGGGGAAAGTGGATCGCTCGGAG
  GAGGCTCTCGATGCATCGAGCCTTGGACTGTCCAGAGAACCGCTGCAGCATGGCCAGCGC
  CCGCCAGAAGAGCTTGGAGTTGCTAATCCGAAGTCCAACATGTGGTTGTGTAGGATCTTA
  CCAAGAAGCACATGAGCACCTGTACTCACTGTATTCAGCACCCTTTTTGTGCTTAGCTCA
  CGCTGGAACTTCTGGATGAAGTTCCCTTCATATTCAAGAAAAACAGTGGCCATGTGATGC
  TGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001486
• Insert Size: 2250 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001486.2, NP_001477.1
• RefSeq Size: 2197 bp
• RefSeq ORF: 1878 bp
• Locus ID: 2646
• Cytogenetics: 2p23.3
• Domains: SIS
• Gene Summary: 'This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq, Jul 2008]'