Non Neuronal Enolase (ENO1) (NM_001428) Human Untagged Clone

CAT#: SC119247

ENO1 (untagged)-Human enolase 1, (alpha) (ENO1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENO1
• Synonyms: ENO1L1; HEL-S-17; MPB1; NNE; PPH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001428, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTATTCTCAAGATCCATGCCAGGGAGATCTTTGACTCTCGCGGGAATCCCACTGTTGAGGTTGATC
  TCTTCACCTCAAAAGGTCTCTTCAGAGCTGCTGTGCCCAGTGGTGCTTCAACTGGTATCTATGAGGCCCT
  AGAGCTCCGGGACAATGATAAGACTCGCTATATGGGGAAGGGTGTCTCAAAGGCTGTTGAGCACATCAAT
  AAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACTGAACGTCACAGAACAAGAGAAGATTGACAAACTGA
  TGATCGAGATGGATGGAACAGAAAATAAATCTAAGTTTGGTGCGAACGCCATTCTGGGGGTGTCCCTTGC
  CGTCTGCAAAGCTGGTGCCGTTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCTGACTTGGCTGGCAAC
  TCTGAAGTCATCCTGCCAGTCCCGGCGTTCAATGTCATCAATGGCGGTTCTCATGCTGGCAACAAGCTGG
  CCATGCAGGAGTTCATGATCCTCCCAGTCGGTGCAGCAAACTTCAGGGAAGCCATGCGCATTGGAGCAGA
  GGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAATATGGGAAAGATGCCACCAATGTGGGGGATGAA
  GGCGGGTTTGCTCCCAACATCCTGGAGAATAAAGAAGGCCTGGAGCTGCTGAAGACTGCTATTGGGAAAG
  CTGGCTACACTGATAAGGTGGTCATCGGCATGGACGTAGCGGCCTCCGAGTTCTTCAGGTCTGGGAAGTA
  TGACCTGGACTTCAAGTCTCCCGATGACCCCAGCAGGTACATCTCGCCTGACCAGCTGGCTGACCTGTAC
  AAGTCCTTCATCAAGGACTACCCAGTGGTGTCTATCGAAGATCCCTTTGACCAGGATGACTGGGGAGCTT
  GGCAGAAGTTCACAGCCAGTGCAGGAATCCAGGTAGTGGGGGATGATCTCACAGTGACCAACCCAAAGAG
  GATCGCCAAGGCCGTGAACGAGAAGTCCTGCAACTGCCTCCTGCTCAAAGTCAACCAGATTGGCTCCGTG
  ACCGAGTCTCTTCAGGCGTGCAAGCTGGCCCAGGCCAATGGTTGGGGCGTCATGGTGTCTCATCGTTCGG
  GGGAGACTGAAGATACCTTCATCGCTGACCTGGTTGTGGGGCTGTGCACTGGGCAGATCAAGACTGGTGC
  CCCTTGCCGATCTGAGCGCTTGGCCAAGTACAACCAGCTCCTCAGAATTGAAGAGGAGCTGGGCAGCAAG
  GCTAAGTTTGCCGGCAGGAACTTCAGAAACCCCTTGGCCAAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001428 unedited
  ATACGACTCACTTAGGGCGGCCGCGACTTCGGCACGAGGCGCGGAAAGTATCTGTGGGTA
  CCCGGAGCCGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTCCGCT
  TTCCTCTCCTAGGCGACGAGACCCAGTGGCTAGAAGTTCACCATGTCTCTTCTCAAGCTC
  CATGCCAGGGAGCTCTTTGACTCTCGCGGGAATCCCACTGTTGAGGTTGATCTCTTCACC
  TCAAAAGGTCTCTTCAGAGCTGCTGTGCCCAGTGGTGCTTCAACTGGTATCTATGAGGCC
  CTAGAGCTCCGGGACAATGATAAGACTCGCTATATGGGGAAGGGTGTCTCAAAGGCTGTT
  GAGCACATCAATAAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACTGAACGTCACAGAA
  CAAGAGAAGATTGACAAACTGATGATCGAGATGGATGGAACAGAAAATAAATCTAAGTTT
  GGTGCGAACGCCATTCTGGGGNGTGTCCCTTGCCGTCTGCAAAGCTGGTGCCGTTGAGAA
  GGGGGTCCCCCTGTACCGCCACATCGCTGACTTGGCTGGCAACTCTGAAGTCATCCTGCC
  AGTCCCGGCGTTCAATGTCATCAATGGCGGTTCTCATGCTGGCAACAAGCTGGCCATGCA
  GGAGTTCATGATCCTNCCAGTCGGTGCAGCAAACTTCAGGGAAGCCATGCGCATTGGAGC
  AGAGGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAAATGGGAANAGAGCCACCAAT
  GTGGGGGATGAAGGGCGGGTTTGCTCCCAACATNNCTGAGATAAAGAANGNCTGNNACTG
  CTGAAGACTGCTATTGGGAAAACTGGCTAACTGAATAAGGTGGCAATCGCATGGACGTAC
  CGCCTTCGATTTTTTTAGGTTGGGGAATTAGACCTTGATTTAAACTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001428 unedited
  NNTTTTTACTCTGGCCCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTCTCAGGGGCCACTGAGGCTTTTTATTTTGAGCACA
  AAACCACCGGGGATCTACCCTGGGGCCACCCCGGAAATGACACGAGGCTCACATGACTCT
  AAACACTTGGGGGAAAGGGAGGCGAAAAAAACAATGACTTGGGCCAATTACACAACTGCA
  AAGCTAGAGCTGCCAACAGGGCTCCAGGGAGCTTGGCTTCTGTAAAAGTTCTAAGGAAGC
  GGTACAAACTCCACGGGGGGGGGGCGCTAACTAGCAGGGACCCCTGCAAGTGTTGGTCGG
  GGGCCTCAAGCTGCCTGAGCTGACACGAGGGGAGGGGTCTGTGTACCCAACAGGTGACCG
  AAGGGCTTGCCTGCCCACAGTTTACTTGGCCAAGGGGTTTCTGAAGTTCCTGCCGGCAAA
  CTTACCCTTGCTGCCCAGCTCCTTTTCAATTCTGAGGAGCTGGTTGTACTTGGCCAAGCG
  CTCAGATCGGCAAGGGGCACCAGTCTTGATCTGCCCAGTGCACAGCCCCACAACCAGGTC
  AGCGATGAAGGTATCTTCAGTCTCCCCCGAACGATGAGACACCATGACGCCCCAACCATT
  GGCCTGGGCCAGCTTGCACGCCTGAAGAGACTCGGCCACGAAGCCAATCTGTTTGACTTT
  GACCAGGAAGCCATTGCAGGACTTTTCGTTCACGGCCTTGAAGATCCTTTTTGGTTTGGC
  ACTGGGAGATCATCCCCCATACCTGAATTCCGCCCTGGCTGGAACCTTTGCCAAGCTCCC
  AATATCCTGGAAAAGGAATCCTTAATAACCCCACTGGTAACCCTGGAAGAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001428
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001428.2, NP_001419.1
• RefSeq Size: 1812 bp
• RefSeq ORF: 1305 bp
• Locus ID: 2023
• Cytogenetics: 1p36.23
• Domains: enolase
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
• Gene Summary: 'This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (1) encodes isoform (1), which is localized to the cytosol, and has alpha-enolase activity. It has been reported that the monomeric form of this isoform functions as a structural lens protein (tau-crystallin), and the dimeric form as an enolase (PMID:2462567).