Non Neuronal Enolase (ENO1) (NM_001428) Human Untagged Clone

CAT#: SC119247

ENO1 (untagged)-Human enolase 1, (alpha) (ENO1), transcript variant 1


  "NM_001428" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ENO1
Synonyms ENO1L1; HEL-S-17; MPB1; NNE; PPH
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001428, the custom clone sequence may differ by one or more nucleotides


ATGTCTATTCTCAAGATCCATGCCAGGGAGATCTTTGACTCTCGCGGGAATCCCACTGTTGAGGTTGATC
TCTTCACCTCAAAAGGTCTCTTCAGAGCTGCTGTGCCCAGTGGTGCTTCAACTGGTATCTATGAGGCCCT
AGAGCTCCGGGACAATGATAAGACTCGCTATATGGGGAAGGGTGTCTCAAAGGCTGTTGAGCACATCAAT
AAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACTGAACGTCACAGAACAAGAGAAGATTGACAAACTGA
TGATCGAGATGGATGGAACAGAAAATAAATCTAAGTTTGGTGCGAACGCCATTCTGGGGGTGTCCCTTGC
CGTCTGCAAAGCTGGTGCCGTTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCTGACTTGGCTGGCAAC
TCTGAAGTCATCCTGCCAGTCCCGGCGTTCAATGTCATCAATGGCGGTTCTCATGCTGGCAACAAGCTGG
CCATGCAGGAGTTCATGATCCTCCCAGTCGGTGCAGCAAACTTCAGGGAAGCCATGCGCATTGGAGCAGA
GGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAATATGGGAAAGATGCCACCAATGTGGGGGATGAA
GGCGGGTTTGCTCCCAACATCCTGGAGAATAAAGAAGGCCTGGAGCTGCTGAAGACTGCTATTGGGAAAG
CTGGCTACACTGATAAGGTGGTCATCGGCATGGACGTAGCGGCCTCCGAGTTCTTCAGGTCTGGGAAGTA
TGACCTGGACTTCAAGTCTCCCGATGACCCCAGCAGGTACATCTCGCCTGACCAGCTGGCTGACCTGTAC
AAGTCCTTCATCAAGGACTACCCAGTGGTGTCTATCGAAGATCCCTTTGACCAGGATGACTGGGGAGCTT
GGCAGAAGTTCACAGCCAGTGCAGGAATCCAGGTAGTGGGGGATGATCTCACAGTGACCAACCCAAAGAG
GATCGCCAAGGCCGTGAACGAGAAGTCCTGCAACTGCCTCCTGCTCAAAGTCAACCAGATTGGCTCCGTG
ACCGAGTCTCTTCAGGCGTGCAAGCTGGCCCAGGCCAATGGTTGGGGCGTCATGGTGTCTCATCGTTCGG
GGGAGACTGAAGATACCTTCATCGCTGACCTGGTTGTGGGGCTGTGCACTGGGCAGATCAAGACTGGTGC
CCCTTGCCGATCTGAGCGCTTGGCCAAGTACAACCAGCTCCTCAGAATTGAAGAGGAGCTGGGCAGCAAG
GCTAAGTTTGCCGGCAGGAACTTCAGAAACCCCTTGGCCAAGTAA


>OriGene 5' read for NM_001428 unedited
ATACGACTCACTTAGGGCGGCCGCGACTTCGGCACGAGGCGCGGAAAGTATCTGTGGGTA
CCCGGAGCCGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTCCGCT
TTCCTCTCCTAGGCGACGAGACCCAGTGGCTAGAAGTTCACCATGTCTCTTCTCAAGCTC
CATGCCAGGGAGCTCTTTGACTCTCGCGGGAATCCCACTGTTGAGGTTGATCTCTTCACC
TCAAAAGGTCTCTTCAGAGCTGCTGTGCCCAGTGGTGCTTCAACTGGTATCTATGAGGCC
CTAGAGCTCCGGGACAATGATAAGACTCGCTATATGGGGAAGGGTGTCTCAAAGGCTGTT
GAGCACATCAATAAAACTATTGCGCCTGCCCTGGTTAGCAAGAAACTGAACGTCACAGAA
CAAGAGAAGATTGACAAACTGATGATCGAGATGGATGGAACAGAAAATAAATCTAAGTTT
GGTGCGAACGCCATTCTGGGGNGTGTCCCTTGCCGTCTGCAAAGCTGGTGCCGTTGAGAA
GGGGGTCCCCCTGTACCGCCACATCGCTGACTTGGCTGGCAACTCTGAAGTCATCCTGCC
AGTCCCGGCGTTCAATGTCATCAATGGCGGTTCTCATGCTGGCAACAAGCTGGCCATGCA
GGAGTTCATGATCCTNCCAGTCGGTGCAGCAAACTTCAGGGAAGCCATGCGCATTGGAGC
AGAGGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAAATGGGAANAGAGCCACCAAT
GTGGGGGATGAAGGGCGGGTTTGCTCCCAACATNNCTGAGATAAAGAANGNCTGNNACTG
CTGAAGACTGCTATTGGGAAAACTGGCTAACTGAATAAGGTGGCAATCGCATGGACGTAC
CGCCTTCGATTTTTTTAGGTTGGGGAATTAGACCTTGATTTAAACTA
>OriGene 3' read for NM_001428 unedited
NNTTTTTACTCTGGCCCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTCTCAGGGGCCACTGAGGCTTTTTATTTTGAGCACA
AAACCACCGGGGATCTACCCTGGGGCCACCCCGGAAATGACACGAGGCTCACATGACTCT
AAACACTTGGGGGAAAGGGAGGCGAAAAAAACAATGACTTGGGCCAATTACACAACTGCA
AAGCTAGAGCTGCCAACAGGGCTCCAGGGAGCTTGGCTTCTGTAAAAGTTCTAAGGAAGC
GGTACAAACTCCACGGGGGGGGGGCGCTAACTAGCAGGGACCCCTGCAAGTGTTGGTCGG
GGGCCTCAAGCTGCCTGAGCTGACACGAGGGGAGGGGTCTGTGTACCCAACAGGTGACCG
AAGGGCTTGCCTGCCCACAGTTTACTTGGCCAAGGGGTTTCTGAAGTTCCTGCCGGCAAA
CTTACCCTTGCTGCCCAGCTCCTTTTCAATTCTGAGGAGCTGGTTGTACTTGGCCAAGCG
CTCAGATCGGCAAGGGGCACCAGTCTTGATCTGCCCAGTGCACAGCCCCACAACCAGGTC
AGCGATGAAGGTATCTTCAGTCTCCCCCGAACGATGAGACACCATGACGCCCCAACCATT
GGCCTGGGCCAGCTTGCACGCCTGAAGAGACTCGGCCACGAAGCCAATCTGTTTGACTTT
GACCAGGAAGCCATTGCAGGACTTTTCGTTCACGGCCTTGAAGATCCTTTTTGGTTTGGC
ACTGGGAGATCATCCCCCATACCTGAATTCCGCCCTGGCTGGAACCTTTGCCAAGCTCCC
AATATCCTGGAAAAGGAATCCTTAATAACCCCACTGGTAACCCTGGAAGAGGG
Restriction Sites NotI-NotI     
ACCN NM_001428
Insert Size 1770 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001428.2, NP_001419.1
RefSeq Size 1812 bp
RefSeq ORF 1305 bp
Locus ID 2023
Cytogenetics 1p36.23
Domains enolase
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
Gene Summary 'This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]'
Transcript Variant: This variant (1) encodes isoform (1), which is localized to the cytosol, and has alpha-enolase activity. It has been reported that the monomeric form of this isoform functions as a structural lens protein (tau-crystallin), and the dimeric form as an enolase (PMID:2462567).

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