ECE1 (NM_001397) Human Untagged Clone

CAT#: SC119260

ECE1 (untagged)-Human endothelin converting enzyme 1 (ECE1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ECE1
• Synonyms: ECE
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001397, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGGGCGTGTGGCCGCCCCCGGTGTCCGCCCTGCTGTCGGCGCTGGGGATGTCGACGTACAAGCGGG
  CCACGCTGGACGAGGAGGACCTGGTGGACTCGCTCTCCGAGGGCGACGCATACCCCAACGGCCTGCAGGT
  GAACTTCCACAGCCCCCGGAGTGGCCAGAGGTGCTGGGCTGCACGGACCCAGGTGGAGAAGCGGCTGGTG
  GTGTTGGTGGTACTTCTGGCGGCAGGACTGGTGGCCTGCTTGGCAGCACTGGGCATCCAGTACCAGACAA
  GATCCCCCTCTGTGTGCCTGAGCGAAGCTTGTGTCTCAGTGACCAGCTCCATCTTGAGCTCCATGGACCC
  CACAGTGGACCCCTGCCATGACTTCTTCAGCTACGCCTGTGGGGGCTGGATCAAGGCCAACCCAGTCCCT
  GATGGCCACTCACGCTGGGGGACCTTCAGCAACCTCTGGGAACACAACCAAGCAATCATCAAGCACCTCC
  TCGAAAACTCCACGGCCAGCGTGAGCGAGGCAGAGAGAAAGGCGCAAGTATACTACCGTGCGTGCATGAA
  CGAGACCAGGATCGAGGAGCTCAGGGCCAAACCTCTAATGGAGTTGATTGAGAGGCTCGGGGGCTGGAAC
  ATCACAGGTCCCTGGGCCAAGGACAACTTCCAGGACACCCTGCAGGTGGTCACCGCCCACTACCGCACCT
  CACCCTTCTTCTCTGTCTATGTCAGTGCCGATTCCAAGAACTCCAACAGCAACGTGATCCAGGTGGACCA
  GTCTGGCCTGGGCTTGCCCTCGAGAGACTATTACCTGAACAAAACTGAAAACGAGAAGGTGCTGACCGGA
  TATCTGAACTACATGGTCCAGCTGGGGAAGCTGCTGGGCGGCGGGGACGAGGAGGCCATCCGGCCCCAGA
  TGCAGCAGATCTTGGACTTTGAGACGGCACTGGCCAACATCACCATCCCACAGGAGAAGCGCCGTGATGA
  GGAGCTCATCTACCACAAAGTGACGGCAGCCGAGCTGCAGACCTTGGCACCCGCCATCAACTGGTTGCCT
  TTTCTCAACACCATCTTCTACCCCGTGGAGATCAATGAATCCGAGCCTATTGTGGTCTATGACAAGGAAT
  ACCTTGAGCAGATCTCCACTCTCATCAACACCACCGACAGATGCCTGCTCAACAACTACATGATCTGGAA
  CCTGGTGCGGAAAACAAGCTCCTTCCTTGACCAGCGCTTTCAGGACGCCGATGAGAAGTTCATGGAAGTC
  ATGTACGGGACCAAGAAGACCTGTCTTCCTCGCTGGAAGTTTTGCGTGAGTGACACAGAAAACAACCTGG
  GCTTTGCGTTGGGCCCCATGTTTGTCAAAGCAACCTTCGCCGAGGACAGCAAGAGCATAGCCACCGAGAT
  CATCCTGGAGATTAAGAAGGCATTTGAGGAAAGCCTGAGCACCCTGAAGTGGATGGATGAGGAAACCCGA
  AAATCAGCCAAGGAAAAGGCCGATGCCATCTACAACATGATAGGATACCCCAACTTCATCATGGATCCCA
  AGGAGCTGGACAAAGTGTTTAATGACTACACTGCAGTTCCAGACCTCTACTTTGAAAATGCCATGCGGTT
  TTTCAACTTCTCATGGAGGGTCACTGCCGATCAGCTCAGGAAAGCCCCCAACAGAGATCAGTGGAGCATG
  ACCCCGCCCATGGTGAACGCCTACTACTCGCCCACCAAGAATGAGATTGTGTTTCCGGCCGGGATCCTGC
  AGGCACCATTCTACACACGCTCCTCACCCAAGGCCTTAAACTTTGGTGGCATAGGTGTCGTCGTGGGCCA
  TGAGCTGACTCATGCTTTTGATGATCAAGGACGGGAGTATGACAAGGACGGGAACCTCCGGCCATGGTGG
  AAGAACTCATCCGTGGAGGCCTTCAAGCGTCAGACCGAGTGCATGGTAGAGCAGTACAGCAACTACAGCG
  TGAACGGGGAGCCGGTGAACGGGCGGCACACCCTGGGGGAGAACATCGCCGACAACGGGGGTCTCAAGGC
  GGCCTATCGGGCTTACCAGAACTGGGTGAAGAAGAACGGGGCTGAGCACTCGCTCCCCACCCTGGGCCTC
  ACCAATAACCAGCTCTTCTTCCTGGGCTTTGCACAGGTCTGGTGCTCCGTCCGCACACCTGAGAGCTCCC
  ACGAAGGCCTCATCACCGATCCCCACAGCCCCTCTCGCTTCCGGGTCATCGGCTCCCTCTCCAATTCCAA
  GGAGTTCTCAGAACACTTCCGCTGCCCACCTGGCTCACCCATGAACCCGCCTCACAAGTGCGAAGTCTGG
  TAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001397 unedited
  CCATTCCCCCGCCCGTTGAAGCAATGGGCGGTAGGCGTGTCCGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGGCGTCTGGGGCACAGCATGCGGGGCGTGTGGCCGCCCCCGGTG
  TCCGCCCTGCTGTCGGCGCTGGGGATGTCGACGTACAAGCGGGCCACGCTGGACGAGGAG
  GACCTGGTGGACTCGCTCTCCGAGGGCGACGCATACCCCAACGGCCTGCAGGTGAACTTC
  CACAGCCCCCGGAGTGGCCAGAGGTGCTGGGCTGCACGGACCCAGGTGGAGAAGCGGCTG
  GTGGTGTTGGTGGTACTTCTGGCGGCAGGACTGGTGGCCTGCTTGGCAGCACTGGGCATC
  CAGTACCAGACAAGATCCCCCTCTGTGTGCCTGAGCGAAGCTTGTGTCTCAGTGACCAGC
  TCCATCTTGAGCTCCATGGACCCCACAGTGGACCCCTGCCATGACTTCTTCAGCTACGCC
  TGTGGGGGCTGGATCAAGGCCAACCCAGTCCCTGATGGCCACTCACGCTGGGGGACCTTC
  AGCAACCTCTGGGAACACAACCAAGCAATCATCAAGCACCTCCTCGAAAACTCCACGGCC
  AGCGTGAGCGAGGCAGAGAGAAAGGCGCANGTATACTACCGTGCGTGCATGAACGAGACC
  AGGATCGAGGAGCTCAGGGCCAAACCTCTAATGGAGTTGATTGAGAGGCTCGGNGGCTGG
  ACATCACAGGTCCCTGNGCCAAGTACAACTTCCAGGACCCCTGCAGGTGGTCACCGNCCA
  CTACCGCACCTCACCCTTNTTCTCTGTCTATGTCAGTGCCGATTCCAAGACTTCACAGCT
  ACGTGATCCCAGTGGACCAGTCTGGCCTGGGCTTGCCCTCGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001397 unedited
  NGGAAATTGAGTTCCGTGTATTCCATATGTTTAAAAAATATTTCCCCAAAATATATCTTG
  AAAGCATTTGACACAGTGGTATTTGTGGCGTATCTGGCGCTTGTCAGTGTGGGGCCCAGG
  CCTGATGAGCCACCAGCCCAGCACCCGAATGCCTGCTGAAGGTGGCGCACACGTGTGCCT
  GGGATGTCCGGCTCTTCACAGGGGATCAGACTGCGGGTCACGTTGAGAGCCAACACCATG
  GGCTCGGTTCCGGCTGAAAACCCGCCAGTGTGAGGAAGCAGGGCCCCGGGTGGCCAAGCG
  GGCTGAGCAATGCCCTGGAGGCTGGATGGGGGTCTCGTCCTCAGCCCCTTCCCCTCCTCC
  GTCTTGGCTCTCTCCGCTTCGTCCTTACCAGACTTCGCACTTGTGAGGCGGGTTCATGGG
  TGAGCCAGGTGGGCAGCGGAAGTGTTCTGAGAACTCCTTGGAATTGGAGAGGGAGCCGAT
  GACCCGGAAGCGAGAGGGGCTGTGGGGATCGGTGATGAGGCCTTCGTGGGAGCTCTCAGG
  TGTGCGGACGGAGCACCAGACCTGTGCAAAGCCCAGGAAGAAGAGCTGGTTATTGGTGAG
  GCCCAGGGTGGGGAGCGAGTGCTCAGCCCCGTTCTTCTTCACCCAGTTCTGGTAAGCCCG
  ATAGGCCGCCTTGAGACCCCCGTTGTCGGCGATGTTCTCCCCCAGGGTGTGCCGCCCGTT
  CACCGGCTCCCCGTTCACGCTGTAGTTGCTGTACTGCTCTACCATGCACTCGGTCTGACG
  CTTGAANGCCTCCACGGATGAGTTTCTCCACCATGGCCGGGAGTTCCCGTCCNTGTCATA
  CTCCCGTCCTTGATCATCAAAGCATGAGTCAGCTCATGGCCCACGACGACACCTATGCCA
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001397
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001397.1, NP_001388.1
• RefSeq Size: 2409 bp
• RefSeq ORF: 2313 bp
• Locus ID: 1889
• Cytogenetics: 1p36.12
• Domains: Peptidase_M13
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: 'The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1 also known as isoform b).